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Job DescriptionP08204
Confidence100.00%DateThu Jan 5 11:00:58 GMT 2012
Rank14Aligned Residues468
% Identity19%Templatec3ezwD_
PDB info PDB header:transferaseChain: D: PDB Molecule:glycerol kinase; PDBTitle: crystal structure of a hyperactive escherichia coli glycerol kinase2 mutant gly230 --> asp obtained using microfluidic crystallization3 devices
Resolution2.00 Å

  Insertion relative to template
  Deletion relative to template
  Catalytic residue from the CSA
 
Detailed help on interpreting your alignment


   2.......10.........20.........30.........40.........50.........60.........70.........80.
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Query Sequence  AIAIGLDFGSDSVRALAVDCATGEEIATSVEWYPRWQKGQFCDAPNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVV
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Template Sequence  KYIVALDQGTTSSRAVVMD. HDANIISVSQREFE. . . . . . QIYPKPGWVEHDPMEIWATQSSTLVEVLAKADISS. DQIA
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   4.....10.........20.. .......30...... ...40.........50.........60.........70. ....
 
   82.......90....... ..100.........110.........120.........130.........140.........150.........160
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Query Sequence  GIGVDSTGSTPAPIDA. DGNVLALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
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Template Sequence  AIGITNQRETTIVWEKETGKPI. . . . . . . . . . . YNAIVWQCRRTAEICEHLKRDG. . . LEDYIRSNTGLVIDPYFSGTKV
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   76...80.........90....... ..100.........110......... 120.........130.........140.
 
   161........170...... ...180.........190 .........200.........210.........220.........230....
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Query Sequence  LHVTRQDSAVAQSAAS. . . . WIELCDWVPALLSG. . TTRPQDIRRGRCSAGHKSLWHESWGGLPPASFFDELDPILNRHL
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Template Sequence  KWILDHVEGSRERARRGELLFGTVDTWLIWKMTQGRVHVTDYTNASR. . . . . . TMLFNIHTLDWDDKMLEVL. . . . . . DI
Template Known Secondary structure  STTTTS
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   142.......150.........160.........170.........180........ .190.........200....... ..
 
   235....240.........250.........260.........270.........280.... .....290.........300.........310...
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Query Sequence  PSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCHMGAVGAGA. QPNALVKVIGTSTCDILIADKQSVGERAV
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Template Sequence  PREMLPEVRRSSEVYGQTN. . . . . . . . . . . RIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNTGEKAVKSENG
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GGG


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   210.........220........ .230.........240.........250.........260.........270........
 
   314.....320.........330.........340.........350.........360.........370.........380.........390...
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Query Sequence  KGICGQVDGSVVPGFIGLEAGQSAFGDIYAWFGRVLGWPLEQLAAQHPELKTQINASQKQLLPALTEAWAKNPSLDHLPV
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Template Sequence  LLT. TIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLIDS. . . . . . . . . . . . . . . . . . . . . . . . . EYFATKVQNTNGVY
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   286.. .290.........300.........310.........320..... ....330.........
 
   394.....400.........410.........420.........430.........440....... ..450.........460.........470..
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Query Sequence  VLDWFNGRRTPNANQRLKGVITDLNLATDAPLLFGGLIAATAFGARAIMECFTD. QGIAVNNVMALGGIARKNQVIMQAC
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Template Sequence  VVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDVLEAMQADSGIRLHALRVDGGA. VANNFLMQFQ
Template Known Secondary structure 

TT
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   344.....350.........360.........370.........380.........390.........400.........410.. .......420..
 
   473......480.........490.........500.........510.........520.........530.........540.........550
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Query Sequence  CDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIPSAQQKMASAVEKTLQPCSEQAQRFEQLYRRYQQWAMSAEQHY
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Template Sequence  SDILGTRVER. . . PEVTALGAAYLAGLAVGFWQNLDELQ. . . . . EIEREFRPGIETTERNYR. YAGWKKAVKRAMAWE
Template Known Secondary structure  TS...

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   423......430.. .......440.........450........ .460.........470...... ...480.........490.
 
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Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
 
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Lawrence Kelley, Benjamin Jefferys 
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