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Job DescriptionP25718
Confidence100.00%DateThu Jan 5 11:42:17 GMT 2012
Rank79Aligned Residues496
% Identity13%Templatec3bc9A_
PDB info PDB header:hydrolaseChain: A: PDB Molecule:alpha amylase, catalytic region; PDBTitle: alpha-amylase b in complex with acarbose
Resolution1.35 Å

  Insertion relative to template
  Deletion relative to template
  Catalytic residue from the CSA
 
Detailed help on interpreting your alignment


   90.........100.........110.........120.........130.........140.........150... ......160.......
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Query Sequence  YTLQIDTRSGTPTLMISIQNAAEPVASLVRECPKWDGLPLTVDVSATFPEGAAVRDYYSQQIAI. . VKNGQIMLQPAATS
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Template Sequence  FLIESEPSTGASVSKNLTEIILIFSNDINKVSQ. . . . . . . . LALTDLITDSDIQGIDYNIEGNKVIINNFSLEPTCNYRL
Template Known Secondary structure 
S
TT
TT

SS
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TT
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TT
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   17..20.........30.........40......... 50.........60.........70.........80........
 
   168.170.........180.........190.........200.........210.........220.........230.........240.......
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Query Sequence  NGLLLLERAETDTSAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMAEIGTFHGGDLRGLTNKLDYLQQLGVNALW
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Template Conservation                                   
                                    
  

 
 

Template Sequence  SYEVIDIYDNHLQGYIEFLVNQSNYPQIPDQEVNHTILQAFYWEMNTGEYATEHPEEANLWNLLAERAPELAEAGFTAVW
Template Known Secondary structure  TT



SS




GGG






TTTTS
GGGGGT

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   8990.........100.........110.........120.........130.........140.........150.........160........
 
   248.250.........260.........270.........280. ........290.........300.........310........
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Query Sequence  ISAPFEQIHGWVGGGTKGDFPHYAYHGYYTQDWT. . . . . . . . . NLDANMGNEADLRTLVDSAHQRGIRILFDVVMNHTGY
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Template Sequence  LPPANKGMAGI. . . . . . . . . . . . HDVGYGTYDLWDLGEFDQKGTVRTKYGTKGELENAIDALHNNDIKVYFDAVLNHRM.
Template Known Secondary structure 



STTGG............G

S
STT
SS
S
BTTBSSBTTB
TT


S
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   169170......... 180.........190.........200.........210.........220.........230.....
 
   319320.........330.........340.........350.........360.........370.........380.........390........
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Query Sequence  ATLADMQEYQFGALYLSGDEVKKSLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNPGFDDLT
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Template Sequence  . . . . GADYAETVLLDENSRDKPGQYIKAWTGFNFPGRNGEYSNFTWNGQCFDGTDWDDYSKESGKYLFDEKSWDWTYNWD
Template Known Secondary structure  ....S
SB
TT
SSSTT
B

TTTTTSS



B
GGGTTTTBTT





SSTT
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   236...240.........250.........260.........270.........280.........290.........300.........310.
 
   399400.........410.........420.........430.........440.........450.........460.........470........
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Query Sequence  MSLAFLPDIKTESTTASGLPVFYKNKMDTHAKAIDGYTPRDYLTHWLSQWVRDYGIDGFRVDTAKHVELPAWQQLKTEAS
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Template Sequence  EDYLMGADVDYE. . . . . . . . . . . . . . . . . . . . . . . NEAVQNDVIDWGQWIINNIDFDGFRLDAVKHIDYRFIDKWMSAVQ
Template Known Secondary structure 
S

SS
TT.......................
TT


TTGGGS
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   312.......320... ......330.........340.........350.........360........
 
   479480.........490.........500.........510..... ....520.........530.........540.... .....550...
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Query Sequence  AALREWKKANPDKALDDKPFWMTGEAWGHGVMQSDYY. . . . RHGFDAMINFDYQEQAAKAVDCLAQMDTT. WQQMAEKLQ
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Template Sequence  NS. . . . . . . . . . . . . SNRDVFFVGEAWVEDVDDLKGFLDTVGNPDLRVFDFPLRSFFVDMLNGAYMADLRNAGLVNSPGY
Template Known Secondary structure  T.............
SS






S

TT
TT


GGGGGS
SGGGSTTT
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   369370 .........380.........390.........400.........410.........420.........430.....
 
   554.....560......... 570.........580.... .....590.........600.........610.........620....
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Query Sequence  GFNVLSYLSSHDTRLF. . . . . . . . REGGDKAAELLLLAP. GAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
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Template Sequence  ENRAVTFVDNHDTDRDEGSYTVSIYSRKYQAYAYILTRAEGVPTVYWKDYYIWEMK. . . . . . . . . . . . . . . . . . . . . . . .
Template Known Secondary structure  GGG
S

TTTS
SSS
SS



SS
SSSTS

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   436...440.........450.........460.........470.........480.........490.
 
   625....630.........640.........650. ........660....... ..670......
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Query Sequence  ASVAHWQKISQFRARHPAIGAGKQTTL. . LLKQGYGFVREHGDDK. . . . VLVVWAGQQ
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Template Sequence  . . . EGLDKLLTARRY. . . YAYGPGYEVDNNDADIYSYVRSGFPDVAGDGLVLMISDGT
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S

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   492.......500... ......510.........520.........530.........540...
 
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No model constructed - rank, confidence too low




Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
 
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Lawrence Kelley, Benjamin Jefferys 
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