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Job DescriptionP25718
Confidence100.00%DateThu Jan 5 11:42:17 GMT 2012
Rank38Aligned Residues410
% Identity21%Templatec3a47A_
PDB info PDB header:hydrolaseChain: A: PDB Molecule:oligo-1,6-glucosidase; PDBTitle: crystal structure of isomaltase from saccharomyces cerevisiae
Resolution1.59 Å

  Insertion relative to template
  Deletion relative to template
  Catalytic residue from the CSA
 
Detailed help on interpreting your alignment


   179180.........190.........200.........210.........220.........230.........240.........250........
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Query Sequence  DTSAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMAEIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGW
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Template Sequence  PETEPKWWKEATFYQIYPASFKDSNDDGW. . . . . . . . . . . . . . . . . . GDMKGIASKLEYIKELGADAIWISPFYDSPQ. .
Template Known Secondary structure  GGGS




GGGS

SSSSSS..................

TT
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   8.10.........20.........30...... ...40.........50.........60.......
 
   259260.........270.........280.........290.........300.........310.........320.........330........
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Query Sequence  VGGGTKGDFPHYAYHGYYTQDWTNLDANMGNEADLRTLVDSAHQRGIRILFDVVMNHTGYATLADMQEYQFGALYLSGDE
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Template Sequence  . . . . . . . . . . . . DDMGYDIANYEKVWPTYGTNEDCFALIEKTHKLGMKFITDLVINHCSSEHEWFKESRSSKTNPKRDWF
Template Known Secondary structure  ............TTTTSS
S
TTT

TT


SB

TTSTT
SSSTTGGGB
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   68.70.........80.........90.........100.........110.........120.........130.....
 
   339340.........350.........360.........370.........380.........390.........400.........410........
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Query Sequence  VKKSLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNPGFDDLTMSLAFLPDIKTESTTASGLP
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Template Sequence  FWRPPKGYDAEGKPIPPNNWKSYFGGSAWTFD. . . . . . . . . . . . . . . . . . . EKTQEFYLRLFCSTQPDLNWE. . . . . . . .
Template Known Secondary structure 


S
TTS

SS
BB
TTSSBS...................TTTT
SSSTT

BB
TT........
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   136...140.........150.........160....... ..170.........180........
 
   419420.........430.........440.........450.........460...... ..
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Query Sequence  VFYKNKMDTHAKAIDGYTPRDYLTHWLSQWVRDYGIDGFRVDTAKHVE. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . LP
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Template Sequence  . . . . . . . . . . . . . . . NEDCRKAIYESAVGYWLDHGVDGFRIDVGSLYSKVVGLPDAPVVDKNSTWQSSDPYTLNGPRIHE
Template Known Secondary structure  ...............
TTT

TTGGGS


TT







TT
SSB

SS
TT
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   189190.........200.........210.........220.........230.........240.........250...
 
   469470.........480.........490.........500.........510..... ....520.........530..... ..
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Query Sequence  AWQQLKTEASAALREWKKANPDKALDDKPFWMTGEAWGHGVMQSDYY. . . . RHGFDAMINFDYQEQAAKAV. . . . . . . DC
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Template Sequence  FHQEMNQFIRN. . . . . . . . . . . RVKDGREIMTVGEMQHASDETKRLYTSASRHELSELFNFSHTDVGTSPLFRYNLVPFE
Template Known Secondary structure  ...........
SS







GGG
S
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   254.....260.... .....270.........280.........290.........300.........310.........320..
 
   538.540.........550.... .....560.........570.. .......580.........590.........600...
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Query Sequence  LAQMDTTWQQMAEKLQG. . FNVLSYLSSHDTRLFREG. . . . . . . . . . . . GDKAAELLLLAPGAVQIFYGDESSRPFGPTG
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Template Conservation                             


                            
  
 
 
  
 
 
       
Template Sequence  LKDWKIALAELFRYINGTDCWSTIYLENHDQPRSITRFGDDSPKNRVISGKLLSVLLSALTGTLYVYQGQELGQINFKNW
Template Known Secondary structure  TTTGGGTTSS



S

TTS

S

STTTT
SSTTGGGT




S
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   323......330.........340.........350.........360.........370.........380.........390.........400..
 
   604 .....610.........620
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Query Sequence  S. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . DPLQGTRSDMNWQDVS. . . . . . . . . . . . . . . . . . . . . . . . . . .
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Template Conservation                                             
 
  
                               
Template Sequence  PVEKYEDVEIRNNYNAIKEEHGENSEEMKKFLEAIALISRDHARTPMQWSREEPNAGFSGPSAKPWFYLNDSFREGINVE
Template Known Secondary structure 
GGG



SSS
GGGGTS






TTTTTSS
TT


SS


GGGGGT
S
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   403......410.........420.........430.........440.........450.........460.........470.........480..
 
   621........630.........640.........650. ........660.........670...
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Query Sequence  . . . GKSAASVAHWQKISQFRARHPAIGAGKQTTL. . . . LLKQGYGFVREHGDDKVLVVWA
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Template Sequence  DEIKDPNSVLNFWKEALKFRKAHKDITVYGYDFEFIDLDNKKLFSFTKKYNNKTLFAALN
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TTSTTS
STT
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   483......490.........500.........510.........520.........530.........540..
 
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No model constructed - rank, confidence too low




Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
 
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Lawrence Kelley, Benjamin Jefferys 
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