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Job DescriptionP25718
Confidence100.00%DateThu Jan 5 11:42:17 GMT 2012
Rank34Aligned Residues538
% Identity17%Templatec2wanA_
PDB info PDB header:hydrolaseChain: A: PDB Molecule:pullulanase; PDBTitle: pullulanase from bacillus acidopullulyticus
Resolution1.65 Å

  Insertion relative to template
  Deletion relative to template
  Catalytic residue from the CSA
 
Detailed help on interpreting your alignment


   15....20.........30.........40.........50........ .60.........70.........80.........90...
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Query Sequence  AVAASWTSPGFPAFSEQGTGTFVSHAQLPKGTRPLTLNFDQQCW. QPADAIKLNQMLSLQPCSNTPPQWRLFRDGEYTLQ
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Template Sequence  SVNHPNQAFPTSSAGVQTNLVQLTLASAPDVTHNLDVAADGYKAHNILPRNVLNLPRYDYSG. . . . . . . . . . . . NDLGNV
Template Known Secondary structure  TTTSTT






TT
SS


TTS
TT

GGGGSGGG



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   254.....260.........270.........280.........290.........300.........310..... ....320.
 
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Query Sequence  IDTRSGTPTLMISIQNAAEPVASLVRECPKWDGLPLTVD. . . . . . . VSATFPEGAAVRDYYSQQIA. . . . . . . IVKNGQI
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Template Sequence  YSK. . . . DATSFRVWAPTASNVQLLLYNSEKGSITKQLEMQKSDNGTWKLQVSGNLENWYYLYQVTVNGTTQTAVDPYAR
Template Known Secondary structure 
S....S
TT
SSSSSS

S

GGGS

TT

SS


TT
S
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   322.. .....330.........340.........350.........360.........370.........380.........390.......
 
   160.........170...... ...180.........190.........200.........210.........220.........230...
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Query Sequence  MLQPAATSNGLLLLERA. . . . . . ETDTSAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMAEIGTFHGGDLRGLTN
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Template Sequence  AISVNATRGMIVDLKATDPAGWQGDHEQTPANPVDEVIYEAHVRDFSIDANSGMKNKGKYL. . . . AFTEHGTKGPDHVKT
Template Known Secondary structure  SBSGGGSS


GGGGS
TTGGG





SSGGG



STTS

SSTTSGG....GGG

S
B
GGG
B
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   398.400.........410.........420.........430.........440.........450........ .460.........470...
 
   234.....240.........250.........260.........270.........280......... 290.........300......
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Query Sequence  KLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYYTQDWTNLDANMGN. . . . . . . EADLRTLVDSAHQRGIR
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Template Sequence  GIDSLKELGITTVQLQPVEEFNSIDETQ. . . . . . PDTYNWGYDPRNYNVPEGAYATTPEGTARITELKQLIQSLHQQRIG
Template Known Secondary structure  T

S

SSS
TTS......TTS




S
GGGSS
SSTTTT
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   474.....480.........490.........500. ........510.........520.........530.........540.......
 
   307..310.........320.........330.........340.........350.........360.........370.........380......
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Query Sequence  ILFDVVMNHTGYATLADMQEYQFGALYLSGDEVKKSLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDI
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Template Sequence  VNMDVVYNHTFDVMVSDFDKIVPQYYYRTDSN. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Template Known Secondary structure 
TT

S
SSSSSTTTTB
B
TT................................................
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   548.550.........560.........570.........
 
   387..390.........400.........410.........420.........430.........440.........450.........460......
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Query Sequence  GDYDNPGFDDLTMSLAFLPDIKTESTTASGLPVFYKNKMDTHAKAIDGYTPRDYLTHWLSQWVRDYGIDGFRVDTAKHVE
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Template Sequence  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . GNYTNGSGGNEFATEHPMAQKFVLDSVNYWVNEYHVDGFRFDLMALLG
Template Known Secondary structure  ................................S
B

TT



B
TTS


TTGGGG
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   580.........590.........600.........610.........620.......
 
   467..470.........480.........490.........500.........510..... ....520.........530...
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Query Sequence  LPAWQQLKTEASAALREWKKANPDKALDDKPFWMTGEAWGHGVMQSDYY. . . . . . . . RHGFDAMINFDYQEQAAK. . . . .
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Template Sequence  KDTMAKISNELHAI. . . . . . . . . . . . . . NPGIVLYGEPWTGGTSGLSSDQLVTKGQQKGLGIGVFNDNIRNGLDGNVFDK
Template Known Secondary structure  ..............
TT

SS
SS

SS
TTTS

TTTTTTTT



TT
T
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   629630.........640.. .......650.........660.........670.........680.........690....
 
   534.....540.........550.... .....560.........570.. .......580.......
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Query Sequence  . . . . . . . . AVDCLAQMDTTWQQMAEKLQG. . FNVLSYLSSHDTRLFREG. . . . . . . . . . . . . . . . GDKAAELLLLAPGAV
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Template Sequence  TAQGFATGDPNQVDVIKNGVIGSIQDFTSAPSETINYVTSHDNMTLWDKILASNPSDTEADRIKMDELAHAVVFTSQGVP
Template Known Secondary structure  T


TTTT
SS
BTTTTTT
SSGGG
S

SSS


TTS
SBS
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   695....700.........710.........720.........730.........740.........750.........760.........770....
 
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Query Sequence  QIFYGDESSRPFGPTGSDPLQGT. RSDMNWQDVSGKSAASVAHWQKISQFRARHPAIGAGK. . . . . . . QTTLLLKQ. GYG
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Template Sequence  FMQGGEEMLRTKGGNDNSYNAGDSVNQFDWSR. KAQFKDVFDYFSSMIHLRNQHPAFRMTTADQIKQNLTFLESPTNTVA
Template Known Secondary structure  TTGGGT


TT
S

TT

S

T.T
GGGG

S



TT
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   775....780.........790.........800...... ...810.........820.........830.........840.........850...
 
   659660... ......670...
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Query Sequence  FVREH. . . . . GDDKVLVVWA
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Template Sequence  FELKNYANHDTWKNIIVMYN
Template Known Secondary structure 
SSGGG
SSS
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   854.....860.........870...
 
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No model constructed - rank, confidence too low




Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
 
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Lawrence Kelley, Benjamin Jefferys 
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