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Job DescriptionP25718
Confidence100.00%DateThu Jan 5 11:42:17 GMT 2012
Rank28Aligned Residues479
% Identity19%Templatec2e8yA_
PDB info PDB header:hydrolaseChain: A: PDB Molecule:amyx protein; PDBTitle: crystal structure of pullulanase type i from bacillus subtilis str.2 168
Resolution2.11 Å

  Insertion relative to template
  Deletion relative to template
  Catalytic residue from the CSA
 
Detailed help on interpreting your alignment


   87..90.........100.........110.........120.........130. ........140.........150.........160.
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Query Sequence  DGEYTLQIDTRSGTPTLMISIQNAAEPVASLVRECPKWDGLPLTV. . . . . DVSATFPEGAAVRDYYSQQIAIVKNGQIML
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Template Sequence  DGELGAVYTA. . . . DHTVFKVWAPAATSAAVKLSHPNKSGRTFQMTRLEKGVYAVTVTGDLHGYEYLFCICN. NSEWMET
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S


S....S
TT
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TTS



GGGS

TT
.TT
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   103......110.. .......120.........130.........140.........150.........160.........170 .......
 
   162.......170.........180...... ...190.........200.........210.........220.........230.
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Query Sequence  QPAATSNGLLLLERAETDTSAPFDW. . . . . . . . . . HNATVYFVLTDRFENGDPSNDQSYGRHKDGMAEIGTFHGGDLRGL
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Template Sequence  VDQYAKAVTVNGEKGVVLRPDQMKWTAPLKPFSHPVDAVIYETHLRDFSIHENSGMINKGKYLALTETDT. . . . QTANGS
Template Known Secondary structure 

TT
SSB
GGG
S















S
GGG


STTS

SSTTSGGGGG
TT
....B
TTS
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   178.180.........190.........200.........210.........220.........230.........240....... ..250...
 
   232.......240.........250.........260.........270.........280......... 290.........300...
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Query Sequence  TNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYYTQDWTNLDANMGN. . . . . . . . EADLRTLVDSAHQR
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Template Sequence  SSGLAYVKELGVTHVELLPVNDFAGVDEEKPLDA. . . . . YNWGYNPLHFFAPEGSYASNPHDPQTRKTELKQMINTLHQH
Template Known Secondary structure  B
T
SS

SSS
TTSGGG
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S
STTSS
SSST
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   254.....260.........270.........280....... ..290.........300.........310.........320........
 
   304.....310.........320.........330.........340.........350.........360.........370.........380...
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Query Sequence  GIRILFDVVMNHTGYATLADMQEYQFGALYLSGDEVKKSLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIR
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Template Sequence  GLRVILDVVFNHVYKRENSPFEKTVPGYFFRHDECG. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Template Known Secondary structure  T

TT

SSGGGSSTTTSB
B
TTS............................................
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   329330.........340.........350.........360....
 
   384.....390.........400.........410.........420.........430.........440.........450.........460...
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Query Sequence  TDIGDYDNPGFDDLTMSLAFLPDIKTESTTASGLPVFYKNKMDTHAKAIDGYTPRDYLTHWLSQWVRDYGIDGFRVDTAK
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Template Sequence  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . KPSNGTGVGNDIASERRMARKFIADCVVYWLEEYNVDGFRFDLLG
Template Known Secondary structure  ...................................SB

TTSSS

B
TTS


TTGG
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   365....370.........380.........390.........400.........
 
   464.....470.........480.........490.........500.........510.........520 .........530...
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Query Sequence  HVELPAWQQLKTEASAALREWKKANPDKALDDKPFWMTGEAWGHGVMQSDYYRHGFD. . . . . . . . AMINFDYQEQAAK. .
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Template Sequence  ILDIDTVLYMKEKATKA. . . . . . . . . . . . . . KPGILLFGEGWDLATPLPHEQKAALANAPRMPGIGFFNDMFRDAVKGNT
Template Known Secondary structure  GSB..............STT







SS
GGGB

GGGGGG
TT



S
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   410.........420...... ...430.........440.........450.........460.........470.....
 
   534.....540.........550. ........560.........570. ........
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Query Sequence  . . . . . . . . . . . AVDCLAQMDTTWQQMAEK. . . . . . . LQGFNVLSYLSSHDTRLFRE. . . . . . . . . . . . . . . . GGDKAAEL
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Template Sequence  FHLKATGFALGNGESAQAVMHGIAGSSGWKALAPIVPEPSQSINYVESHDNHTFWDKMSFALPQENDSRKRSRQRLAVAI
Template Known Secondary structure  SSTT


GGGT
GGGBTTS
BTTB

SSSSGGG
S

SSSS

TT

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   476...480.........490.........500.........510.........520.........530.........540.........550.....
 
   580.........590.........600......... 610.........620.........630.........640......... 650
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Query Sequence  LLLAPGAVQIFYGDESSRPFGPTGSDPLQG. TRSDMNWQDVSGKSAASVAHWQKISQFRARHPAIGAGKQT. . . . . . . . T
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Template Sequence  ILLAQGVPFIHSGQEFFRTKQGVENSYQSSDSINQLDWDR. RETFKEDVHYIRRLISLRKAHPAFRLRSAADIQRHLECL
Template Known Secondary structure  TSBSTTGGGT


TT
S

TT

S

.T
GGGG

S
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   556...560.........570.........580.........590..... ....600.........610.........620.........630....
 
   651........660..... ....670...
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Query Sequence  LLLKQGYGFVREHGD. . . . . DKVLVVWA
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Template Sequence  TLKEHLIAYRLYDLDEVDEWKDIIVIHH
Template Known Secondary structure 
SS


TTT
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   635....640.........650.........660..
 
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No model constructed - rank, confidence too low




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Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
 
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Lawrence Kelley, Benjamin Jefferys 
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