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Job DescriptionP25718
Confidence100.00%DateThu Jan 5 11:42:17 GMT 2012
Rank9Aligned Residues497
% Identity23%Templatec2d0gA_
PDB info PDB header:hydrolaseChain: A: PDB Molecule:alpha-amylase i; PDBTitle: crystal structure of thermoactinomyces vulgaris r-47 alpha-2 amylase 1 (tvai) mutant d356n/e396q complexed with p5, a3 pullulan model oligosaccharide
Resolution2.60 Å

  Insertion relative to template
  Deletion relative to template
  Catalytic residue from the CSA
 
Detailed help on interpreting your alignment


   71........80.........90.........100.........110.........120.........130.........140.........150
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Query Sequence  SLQPCSNTPPQWRLFRDGEYTLQIDTRSGTPTLMISIQNAAEPVASLVRECPKWDGLPLTVDVSATFPEGAAVRDYYSQQ
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Template Sequence  APEPTSTQSVTLKLRT. . . . . . . FKGDITSANIKYWDTADNAFHWVPMVWDSNDPTGTF. . DYWKGTIPASPSIKYYRFQ
Template Known Secondary structure 
SS

TT

.......TT

STTTT

TTSS..


SS
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   23......30........ .40.........50.........60.........70.... .....80.........90...
 
   151........160.........170......... 180.........190.........200.........210..
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Query Sequence  IAIVKNGQIMLQPAATSNGLLLLERAETD. TSAPFDWHNATVYFVLTDRFENGDPSNDQSYGR. . . . . . . . . . . . . . . . .
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Template Sequence  INDGTSTAWYNGNGPSSTEPNADDFYIIPNFKTPDWLKNGVMYQIFPDRFYNGDSSNDVQTGSYTYNGTPTEKKAWGSSV
Template Known Secondary structure  TTTTSS

SS


TT






GGGT

S
GGG


TTS
BSSS
B


TTS

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   94.....100.........110.........120.........130.........140.........150.........160.........170...
 
   213......220.........230.........240 .........250.........260.........270.........280.........290
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Query Sequence  . HKDGMAEIGTFHGGDLRGLTNKLDYLQQ. LGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYYTQDWTNLDANMGNE
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Template Sequence  YADPGYDNSLVFFGGDLAGIDQKLGYIKKTLGANILYLNPIFKAP. . . . . . . . . . . . . . . TNHKYDTQDYMAVDPAFGDN
Template Known Secondary structure  S

TTS
GGG



TTTT


S


S...............SSS

S
S
TTT

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   174.....180.........190.........200.........210........ .220.........230........
 
   291........300.... .....310.........320.........330.........340.........350.........360......
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Query Sequence  ADLRTLVDSAHQRG. . . . IRILFDVVMNHTGYATLADMQEYQFGALYLSGDEVKKSLGERWSDWKPAAGQTWHSFNDYIN
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Template Sequence  STLQTLINDIHSTANGPKGYLILDGVFNHTGDSHPWFDKYNNFSSQGAYESQSSPWYNYYTFY. . . . . . . . . . . . . . . . .
Template Known Secondary structure 
SSSSS



SB

TTSTTT
TT

SSS

TTT
TT
TTGGGB
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   239240.........250.........260.........270.........280.........290.........300.
 
   367..370.........380.........390.........400.........410.........420.........430.........440......
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Query Sequence  FSDKTGWDKWWGKNWIRTDIGDYDNPGFDDLTMSLAFLPDIKTESTTASGLPVFYKNKMDTHAKAIDGYTPRDYLTHWLS
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Template Sequence  . TWPDSYASFLG. . . . . . . . . . . . . . . . . . . . . . FNSLPKLNYGNS. . . . . . . . . . . . . . . . . . . . . GSAVRGVIYNNSN
Template Known Secondary structure  .TTT
TT......................BB


ST.....................T
TTSTT
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   302.......310.. .......320.... .....330.......
 
   447..450.........460....... ..470.........480.........490.........500.........510
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Query Sequence  . . . . . QWVRDYGIDGFRVDTAKHVEL. . . . . . . . . . . PAWQQLKTEASAALREWKKANPDKALDDKPFWMTGEAWGHGVM
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Template Sequence  SVAKTYLNPPYSVDGWRLNAAQYVDANGNNGSDVTNHQIWSEFRNAVKGV. . . . . . . . . . . . . . NSNAAIIGQYWGNANP
Template Known Secondary structure  SSTTT


TTGGG

STT


SS..............
TT


SS

GG
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   338.340.........350.........360.........370.........380....... ..390.........400...
 
   511........520.........530... ......540.........550.........560.........570.
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Query Sequence  QSDYYRHGFDAMINFDYQEQAAK. . . . . . . . . . . . . . . AVDCLAQMDTTWQQMAEKLQGFNVLSYLSSHDTRLFRE. . . .
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Template Sequence  WTAQ. GNQWDAATNFDGFTQPVSEWITGKDYQNNSASISTTQFDSWLRGTRANYPTNVQQSMMNFLSNHDITRFATRSGG
Template Known Secondary structure  GTTT.SSS
SB
TTTTSB
TTS
B



TTS
TT
S

TTS

TTT
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   404... ..410.........420.........430.........440.........450.........460.........470.........480..
 
   572.......580.........590.........600.........610.........620.........630.........640........
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Query Sequence  . . . GGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSAASVAHWQKISQFRARHPAIGAGKQ
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Template Sequence  DLWKTYLALIFQMTYVGTPTIYYGDEYGMQGGADPDN. . . . . RRSFDWSQ. ATPSNSAVALTQKLITIRNQYPALRTGSF
Template Known Secondary structure 
TTSSSTTTTTT



STTTT.....S



TTT.SSTTS
S
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   483......490.........500.........510......... 520....... ..530.........540.........550......
 
   649650..... ....660.........670...
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Query Sequence  TTLLLKQ. . . GYGFVREHGDDKVLVVWA
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Template Sequence  MTLITDDTNKIYSYGRFDNVNRIAVVLN
Template Known Secondary structure  TTTT
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   557..560.........570.........580....
 
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Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
 
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Lawrence Kelley, Benjamin Jefferys 
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