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Job DescriptionP25718
Confidence100.00%DateThu Jan 5 11:42:17 GMT 2012
Rank18Aligned Residues394
% Identity23%Templatec2aaaA_
PDB info PDB header:glycosidaseChain: A: PDB Molecule:alpha-amylase; PDBTitle: calcium binding in alpha-amylases: an x-ray diffraction2 study at 2.1 angstroms resolution of two enzymes from3 aspergillus
Resolution2.10 Å

  Insertion relative to template
  Deletion relative to template
  Catalytic residue from the CSA
 
Detailed help on interpreting your alignment


   182.......190.........200.........210.........220.........230.........240.........250.........260.
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Query Sequence  APFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMAEIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGG
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Template Sequence  SAASWRTQSIYFLLTDRFGRTDNSTTATCNTGNEI. . . . . . YCGGSWQGIIDHLDYIEGMGFTAIWISPITEQLPQDTAD
Template Known Secondary structure 
TT





TT

S




GGG
S......




TTTT








BTT
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   2.......10.........20.........30...... ...40.........50.........60.........70.....
 
   262.......270.........280.........290.........300.........310.........320.........330.........340.
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Query Sequence  GTKGDFPHYAYHGYYTQDWTNLDANMGNEADLRTLVDSAHQRGIRILFDVVMNHTGYATLADMQEYQFGALYLSGDEVKK
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Template Sequence  GE. . . . . . . AYHGYWQQKIYDVNSNFGTADNLKSLSDALHARGMYLMVDVVPDHMGYAGNGNDVDYSVF. . . . . . . . . . .
Template Known Secondary structure  B
.......STTS
S
TTT

TTT


SB

BSS
GGG

GGGS...........
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   76. ..80.........90.........100.........110.........120.........130.......
 
   342.......350.........360.........370.........380.........390.........400.........410.........420.
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Query Sequence  SLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNPGFDDLTMSLAFLPDIKTESTTASGLPVFY
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Template Sequence  . . . . . . . . . . . . . . . . . . . . . . . . . . . DPFDSSSYFHPYCLITDWDNLTMVEDCWEGDTIVSLPDLDTT. . . . . . . . . . .
Template Known Secondary structure  ...........................BS

SGGGB



B


TT
SB
SSS
BB
TT...........
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   138.140.........150.........160.........170.........
 
   422.......430.........440.........450.........460.........470.........480.........490.........500.
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Query Sequence  KNKMDTHAKAIDGYTPRDYLTHWLSQWVRDYGIDGFRVDTAKHVELPAWQQLKTEASAALREWKKANPDKALDDKPFWMT
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Template Sequence  . . . . . . . . . . . . ETAVRTIWYDWVADLVSNYSVDGLRIDSVLEVQPDFFPGYNKAS. . . . . . . . . . . . . . . . . . . GVYCV
Template Known Secondary structure  ............ST

S
STTS
GGG...................TS
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   180.........190.........200.........210.........220... .....
 
   502.......510.........520.........530....... ..540.........550.........560......... 570
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Query Sequence  GEAWGHGVMQSDYYRHGFDAMINFDYQEQAAKAVDC. . . . . LAQMDTTWQQMAEKLQGFNVLSYLSSHDTRLF. . . . . . R
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Template Sequence  GEIDNGNPASDCPYQKVLDGVLNYPIYWQLLYAFESSSGSISNLYNMIKSVASDCSDPTLLGNFIENHDNPRFAKYTSDY
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S
GGGGGTSSB
SSTTS

S
GGGS
S

TTS

GGGT


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   229230.........240.........250.........260.........270.........280.........290.........300........
 
   571........580.........590.........600.........610.........620.........630.........640.......
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Query Sequence  EGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSAASVAHWQKISQFRARHPAIGAGK. . .
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Template Sequence  SQAKNVLSYIFLSDGIPIVYAGEEQHYAGGKVPYN. . . . . REATWLSG. YDTSAELYTWIATTNAIRKLAIAADSAYITY
Template Known Secondary structure  SSSTTTTTT



TTTTT.....


GGGGT.T
TT

TTTTTS
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   309310.........320.........330.........340... ......350. ........360.........370.........380..
 
   648.650.........660.........670....
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Query Sequence  . . QTTLLLKQGYGFVREHGDDKVLVVWAG
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Template Sequence  ANDAFYTDSNTIAMAKGTSGSQVITVLSN
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TTSSTTT

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   383......390.........400.........410.
 
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Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
 
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Lawrence Kelley, Benjamin Jefferys 
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