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Job DescriptionP25718
Confidence100.00%DateThu Jan 5 11:42:17 GMT 2012
Rank32Aligned Residues500
% Identity21%Templatec1bf2A_
PDB info PDB header:hydrolaseChain: A: PDB Molecule:isoamylase; PDBTitle: structure of pseudomonas isoamylase
Resolution2.00 Å

  Insertion relative to template
  Deletion relative to template
  Catalytic residue from the CSA
 
Detailed help on interpreting your alignment


   8990.........100.........110.........120.........130. ........ 140.........150..
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Query Sequence  EYTLQIDTRSGTPTLMISIQNAAEPVASLVRECPKWDGLPLTV. . . . . . . . DVSATFPE. . . . . . . GAAVRDYYSQQIA.
Query Conservation                                             ........        .......             .
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Template Conservation 



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Template Sequence  SLGASYDAQQA. . NITFRVYSSQATRIVLYLYSAGYGVQESATYTLSPAGSGVWAVTVPVSSIKAAGITGAVYYGYRAWG
Template Known Secondary structure 


TTSS..
SS
SSSSSS


S

STTTT

S

B
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   6...10...... ...20.........30.........40.........50.........60.........70.........80...
 
   153......160.... .....170...
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Query Sequence  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . IVKNGQIMLQPA. . . . . . . . . . . . . . . . . . . . . . . . . . ATSNGLLLL
Query Conservation  .................................            ..........................         
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Template Conservation                                     



                                         
Template Sequence  PNWPYASNWGKGSQAGFVSDVDANGDRFNPNKLLLDPYAQEVSQDPLNPSNQNGNVFASGASYRTTDSGIYAPKGVVLVP
Template Known Secondary structure  TTB


TT

TT

TT


SS
TT




TTS
B

TT
S
BS

SS
SS


GGGGB
STTTTT


TTTS





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   84.....90.........100.........110.........120.........130.........140.........150.........160...
 
   174.....180.........190.........200.........210.........220.........230.........240.........250...
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Query Sequence  ERAETDTSAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMAEIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFE
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Template Sequence  STQSTGTKPTRAQKDDVIYEVHVRGFTEQDTSIPA. . . . . . . . . . . . . . QYRGTYYGAGLKASYLASLGVTAVEFLPVQE
Template Known Secondary structure 











GGG


TT
TTS
G..............GGTTSTT

S

B
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   164.....170.........180.........190........ .200.........210.........220.........
 
   254.....260.........270.........280......... 290.........300.........310.........320......
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Query Sequence  QIHGWVGGGTKGDFPHYAYHGYYTQDWTNLDANMGN. . . . . . . EADLRTLVDSAHQRGIRILFDVVMNHTGYATLADMQE
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Template Sequence  TQNDANDVVPNSD. ANQNYWGYMTENYFSPDRRYAYNKAAGGPTAEFQAMVQAFHNAGIKVYMDVVYNHTAEGGTWTS. .
Template Known Secondary structure  BS
TTTTSSTT

.TT


S


BS
SS
B
GGG
S

STTTT


SS
TT
SBSSS..
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   230.........240.. .......250.........260.........270.........280.........290.........300......
 
   327..330.........340.........350.........360.........370.........380.........390.........400......
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Query Sequence  YQFGALYLSGDEVKKSLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNPGFDDLTMSLAFLPD
Query Conservation                                                     
 
                       


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Template Conservation  ....................................                   
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Template Sequence  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . SDPTTATIYSWRGLDNATYYELT. . . . . . SGNQYFYDNTGIGAN
Template Known Secondary structure  ....................................S
SS
BB
SSB
B
......TTSSSB

SSSSS

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   307..310.........320......... 330.........340....
 
   407..410.........420.........430.........440.........450.........460.........470.........480
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Query Sequence  IKTESTTASGLPVFYKNKMDTHAKAIDGYTPRDYLTHWLSQWVRDYGIDGFRVDTAKHVELPAWQQLKTEASAA. . . . . .
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Template Conservation 

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Template Sequence  FNTY. . . . . . . . . . . . . . . . . . . . . . . NTVAQNLIVDSLAYWANTMGVDGFRFDLASVLGNSCLNGAYTASAPNCPNGGY
Template Known Secondary structure  B
TT.......................STS


TTGGGGGB

SSSS

TTSTT
TT
S
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   345... .350.........360.........370.........380.........390.........400.
 
   481........490.........500.........510...... ...520.........530.........540........
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Query Sequence  . . . . . . . . . . . LREWKKANPDKALDDKPFWMTGEAWGHGVMQSDYYR. HGFDAMINFDYQEQAAKAVDCLAQMDTTWQQM
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Template Conservation                                



                     
    
                   
Template Sequence  NFDAADSNVAINRILREFTVRPAAGGSGLDLFAEPWAIGGNSYQLGGFPQGWSEWNGLFRDSLRQAQNELGSMTIYVTQD
Template Known Secondary structure 
B
TT
TTSS
B

TT

SSB



SSTT


TT
S
TT


BTTB


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   402.......410.........420.........430.........440.........450.........460.........470.........480.
 
   549550.... .....560......... 570..
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Query Sequence  AEKLQG. . . . . . . . . . . . FNVLSYLSSHDTRLF. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . REG
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Template Conservation     
                 



 


   
 
                                             
Template Sequence  ANDFSGSSNLFQSSGRSPWNSINFIDVHDGMTLKDVYSCNGANNSQAWPYGPSDGGTSTNYSWDQGMSAGTGAAVDQRRA
Template Known Secondary structure  TT
GGGT

GGG
S

SSS

TT
SS





TTS






S





TTTTTTS

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   482.......490.........500.........510.........520.........530.........540.........550.........560.
 
   573......580.........590......... 600.........610.........620.........630.........640.........650.
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Query Sequence  GDKAAELLLLAPGAVQIFYGDESSRPF. GPTGSDPLQGTRSDMNWQDVSGKSAASVAHWQKISQFRARHPAIGAGKQTTL
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Template Conservation     
  
 

 

 
 

 


 
    
               
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Template Sequence  ARTGMAFEMLSAGTPLMQGGDEYLRTLQCNNNAYNLDSSANWLTYSW. TTDQSNFYTFAQRLIAFRKAHPALRPSSWYSG
Template Known Secondary structure  SSSTTGGGT


TT
S

TT

STTTS




.

GGGS
SS


T
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   562.......570.........580.........590.........600........ .610.........620.........630.........640
 
   652 .......660... ......670...
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Query Sequence  L. . . . . . . . . . . . . . . . . . . . LKQGYGFVREH. . . . GDDKVLVVWA
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Template Conservation                          


  
           


 
Template Sequence  SQLTWYQPSGAVADSNYWNNTSNYAIAYAINGPSLGDSNSIYVAYN
Template Known Secondary structure  TT
TTSSB

T
TT


GGGGT
SS
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   641........650.........660.........670.........680......
 
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No model constructed - rank, confidence too low




Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
 
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Lawrence Kelley, Benjamin Jefferys 
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