Secondary structure and disorder prediction

		1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	F	H	R	L	L	I	A	T	V	V	G	I	L	A	A	F	A	V	A	G	F	R	H	A	M	L	L	L	E	W	L	F	L	N	N	D	S	G	S	L	V	N	A	A	T	N	L	S	P	W	R	R	L	L	T	P	A	L	G
Secondary structure
SS confidence
Disorder		?																																						?	?	?		?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		G	L	A	A	G	L	L	L	M	G	W	Q	K	F	T	Q	Q	R	P	H	A	P	T	D	Y	M	E	A	L	Q	T	D	G	Q	F	D	Y	A	A	S	L	V	K	S	L	A	S	L	L	V	V	T	S	G	S	A	I	G	R	E
Secondary structure
SS confidence
Disorder														?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		G	A	M	I	L	L	A	A	L	A	A	S	C	F	A	Q	R	F	T	P	R	Q	E	W	K	L	W	I	A	C	G	A	A	A	G	M	A	A	A	Y	R	A	P	L	A	G	S	L	F	I	A	E	V	L	F	G	T	M	M	L
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		A	S	L	G	P	V	I	I	S	A	V	V	A	L	L	V	S	N	L	I	N	H	S	D	A	L	L	Y	N	V	Q	L	S	V	T	V	Q	A	R	D	Y	A	L	I	I	S	T	G	V	L	A	G	L	C	G	P	L	L	L	T
Secondary structure
SS confidence
Disorder																																	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300
Sequence		L	M	N	A	C	H	R	G	F	V	S	L	K	L	A	P	P	W	Q	L	A	L	G	G	L	I	V	G	L	L	S	L	F	T	P	A	V	W	G	N	G	Y	S	T	V	Q	S	F	L	T	A	P	P	L	L	M	I	I	A	G
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	310	.	.	.	.	.	.	.	.	.	320	.	.	.	.	.	.	.	.	.	330	.	.	.	.	.	.	.	.	.	340	.	.	.	.	.	.	.	.	.	350	.	.	.	.	.	.	.	.	.	360
Sequence		I	F	L	C	K	L	C	A	V	L	A	S	S	G	S	G	A	P	G	G	V	F	T	P	T	L	F	I	G	L	A	I	G	M	L	Y	G	R	S	L	G	L	W	F	P	D	G	E	E	I	T	L	L	L	G	L	T	G	M	A
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	370	.	.	.	.	.	.	.	.	.	380	.	.	.	.	.	.	.	.	.	390	.	.	.	.	.	.	.	.	.	400	.	.	.	.	.	.	.	.	.	410	.	.	.	.	.	.	.	.
Sequence		T	L	L	A	A	T	T	H	A	P	I	M	S	T	L	M	I	C	E	M	T	G	E	Y	Q	L	L	P	G	L	L	I	A	C	V	I	A	S	V	I	S	R	T	L	H	R	D	S	I	Y	R	Q	H	T	A	Q	H	S
Secondary structure
SS confidence
Disorder																																																								?	?	?	?
Disorder confidence

Confidence Key
High(9)											Low (0)

?	Disordered
	Alpha helix
	Beta strand

Domain analysis

Hover over an aligned region to see model and summary info

Please note, only up to the top 20 hits are modelled to reduce computer load

Rank Aligned region

PDB 2ht2 chain B Region: 1 - 416 Aligned: 411 Modelled: 416 Confidence: 100.0% Identity: 24% PDB header:membrane protein Chain: B: PDB Molecule:h(+)/cl(-) exchange transporter clca; PDBTitle: structure of the escherichia coli clc chloride channel2 y445h mutant and fab complex Phyre2 PDB 1ots chain A Region: 1 - 417 Aligned: 412 Modelled: 417 Confidence: 100.0% Identity: 24% Fold: Clc chloride channel Superfamily: Clc chloride channel Family: Clc chloride channel Phyre2 PDB 1kpl chain A Region: 1 - 417 Aligned: 412 Modelled: 417 Confidence: 100.0% Identity: 24% Fold: Clc chloride channel Superfamily: Clc chloride channel Family: Clc chloride channel Phyre2 PDB 3nd0 chain A Region: 3 - 416 Aligned: 409 Modelled: 414 Confidence: 100.0% Identity: 24% PDB header:transport protein Chain: A: PDB Molecule:sll0855 protein; PDBTitle: x-ray crystal structure of a slow cyanobacterial cl-/h+ antiporter Phyre2 PDB 3org chain B Region: 3 - 417 Aligned: 384 Modelled: 400 Confidence: 100.0% Identity: 20% PDB header:transport protein Chain: B: PDB Molecule:cmclc; PDBTitle: crystal structure of a eukaryotic clc transporter Phyre2 PDB 2k47 chain A Region: 116 - 145 Aligned: 30 Modelled: 30 Confidence: 13.8% Identity: 17% PDB header:replication Chain: A: PDB Molecule:phosphoprotein; PDBTitle: solution structure of the c-terminal n-rna binding domain2 of the vesicular stomatitis virus phosphoprotein Phyre2 PDB 2knc chain A Region: 222 - 256 Aligned: 35 Modelled: 35 Confidence: 10.6% Identity: 23% PDB header:cell adhesion Chain: A: PDB Molecule:integrin alpha-iib; PDBTitle: platelet integrin alfaiib-beta3 transmembrane-cytoplasmic2 heterocomplex Phyre2 1 c2ht2B_ 2 d1otsa_ 3 d1kpla_ 4 c3nd0A_ 5 c3orgB_ 6 c2k47A_ 7 c2kncA_

Detailed template information

# Template Alignment Coverage 3D Model Confidence % i.d. Template Information

1 c2ht2B_ 100.0 24 PDB header:membrane protein Chain: B: PDB Molecule:h(+)/cl(-) exchange transporter clca; PDBTitle: structure of the escherichia coli clc chloride channel2 y445h mutant and fab complex 2 d1otsa_ 100.0 24 Fold:Clc chloride channel Superfamily:Clc chloride channel Family:Clc chloride channel 3 d1kpla_ 100.0 24 Fold:Clc chloride channel Superfamily:Clc chloride channel Family:Clc chloride channel 4 c3nd0A_ 100.0 24 PDB header:transport protein Chain: A: PDB Molecule:sll0855 protein; PDBTitle: x-ray crystal structure of a slow cyanobacterial cl-/h+ antiporter 5 c3orgB_ 100.0 20 PDB header:transport protein Chain: B: PDB Molecule:cmclc; PDBTitle: crystal structure of a eukaryotic clc transporter 6 c2k47A_ 13.8 17 PDB header:replication Chain: A: PDB Molecule:phosphoprotein; PDBTitle: solution structure of the c-terminal n-rna binding domain2 of the vesicular stomatitis virus phosphoprotein 7 c2kncA_ 10.6 23 PDB header:cell adhesion Chain: A: PDB Molecule:integrin alpha-iib; PDBTitle: platelet integrin alfaiib-beta3 transmembrane-cytoplasmic2 heterocomplex

Binding site prediction

Due to computational demand, binding site predictions are not run for batch jobs

If you want to predict binding sites, please manually submit your model of choice to 3DLigandSite

Transmembrane helix prediction

Transmembrane helices have been predicted in your sequence to adopt the topology shown below

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Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
If you use the binding site predictions from 3DLigandSite, please also cite:
3DLigandSite: predicting ligand-binding sites using similar structures.
Wass MN, Kelley LA and Sternberg MJ Nucleic Acids Research 38, W469-73 (2010) [PubMed]
© Structural Bioinformatics Group Imperial College London Lawrence Kelley, Benjamin Jefferys Disclaimer Terms and Conditions	Component software Template detection: HHpred 1.51 Secondary structure prediction: Psi-pred 2.5 Disorder prediction: Disopred 2.4 Transmembrane prediction: Memsat_SVM Multi-template modelling and ab initio: Poing 1.0