Secondary structure and disorder prediction

		1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	D	W	Y	L	K	V	L	K	N	Y	V	G	F	R	G	R	A	R	R	K	E	Y	W	M	F	I	L	V	N	I	I	F	T	F	V	L	G	L	L	D	K	M	L	G	W	Q	R	A	G	G	E	G	I	L	T	T	I	Y	G
Secondary structure
SS confidence
Disorder		?																																													?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		I	L	V	F	L	P	W	W	A	V	Q	F	R	R	L	H	D	T	D	R	S	A	W	W	A	L	L	F	L	I	P	F	I	G	W	L	I	I	I	V	F	N	C	Q	A	G	T	P	G	E	N	R	F	G	P	D	P	K	L	E
Secondary structure
SS confidence
Disorder																																																		?	?	?	?	?		?	?	?	?	?	?
Disorder confidence
		.
Sequence		P
Secondary structure
SS confidence
Disorder		?
Disorder confidence

Confidence Key
High(9)											Low (0)

?	Disordered
	Alpha helix
	Beta strand

Domain analysis

Hover over an aligned region to see model and summary info

Please note, only up to the top 20 hits are modelled to reduce computer load

Rank Aligned region

PDB 1rh5 chain B Region: 1 - 55 Aligned: 55 Modelled: 55 Confidence: 12.1% Identity: 15% Fold: Single transmembrane helix Superfamily: Preprotein translocase SecE subunit Family: Preprotein translocase SecE subunit Phyre2 PDB 2dyo chain B Region: 70 - 79 Aligned: 10 Modelled: 10 Confidence: 11.2% Identity: 40% PDB header:protein turnover/protein turnover Chain: B: PDB Molecule:autophagy protein 16; PDBTitle: the crystal structure of saccharomyces cerevisiae atg5-2 atg16(1-57) complex Phyre2 PDB 2l2t chain A Region: 51 - 74 Aligned: 24 Modelled: 24 Confidence: 10.7% Identity: 21% PDB header:membrane protein Chain: A: PDB Molecule:receptor tyrosine-protein kinase erbb-4; PDBTitle: solution nmr structure of the erbb4 dimeric membrane domain Phyre2 PDB 2f22 chain A domain 1 Region: 2 - 20 Aligned: 19 Modelled: 19 Confidence: 9.6% Identity: 37% Fold: DinB/YfiT-like putative metalloenzymes Superfamily: DinB/YfiT-like putative metalloenzymes Family: DinB-like Phyre2 PDB 1bzg chain A Region: 75 - 81 Aligned: 7 Modelled: 7 Confidence: 8.9% Identity: 57% PDB header:hormone Chain: A: PDB Molecule:parathyroid hormone-related protein; PDBTitle: the solution structure of human parathyroid hormone-related2 protein (1-34) in near-physiological solution, nmr, 303 structures Phyre2 PDB 1zcz chain A Region: 112 - 117 Aligned: 6 Modelled: 6 Confidence: 8.8% Identity: 50% PDB header:transferase/hydrolase Chain: A: PDB Molecule:bifunctional purine biosynthesis protein purh; PDBTitle: crystal structure of phosphoribosylaminoimidazolecarboxamide2 formyltransferase / imp cyclohydrolase (tm1249) from thermotoga3 maritima at 1.88 a resolution Phyre2 PDB 3skd chain A Region: 75 - 80 Aligned: 6 Modelled: 6 Confidence: 8.6% Identity: 50% PDB header:hydrolase Chain: A: PDB Molecule:putative uncharacterized protein tthb187; PDBTitle: crystal structure of the thermus thermophilus cas3 hd domain in the2 presence of ni2+ Phyre2 PDB 2lkj chain A Region: 114 - 121 Aligned: 8 Modelled: 8 Confidence: 7.6% Identity: 50% PDB header:cell adhesion Chain: A: PDB Molecule:integrin alpha-m; PDBTitle: structures and interaction analyses of the integrin alpha-m beta-22 cytoplasmic tails Phyre2 PDB 1lir chain A Region: 73 - 80 Aligned: 8 Modelled: 8 Confidence: 6.8% Identity: 63% Fold: Knottins (small inhibitors, toxins, lectins) Superfamily: Scorpion toxin-like Family: Short-chain scorpion toxins Phyre2 PDB 2ogi chain A Region: 75 - 80 Aligned: 6 Modelled: 6 Confidence: 6.7% Identity: 50% PDB header:hydrolase Chain: A: PDB Molecule:hypothetical protein sag1661; PDBTitle: crystal structure of a putative metal dependent phosphohydrolase2 (sag1661) from streptococcus agalactiae serogroup v at 1.85 a3 resolution Phyre2 PDB 3zrk chain Y Region: 67 - 76 Aligned: 10 Modelled: 10 Confidence: 6.4% Identity: 40% PDB header:transferase/peptide Chain: Y: PDB Molecule:proto-oncogene frat1; PDBTitle: identification of 2-(4-pyridyl)thienopyridinones as gsk-3beta2 inhibitors Phyre2 PDB 2o08 chain B Region: 75 - 80 Aligned: 6 Modelled: 6 Confidence: 6.3% Identity: 33% PDB header:hydrolase Chain: B: PDB Molecule:bh1327 protein; PDBTitle: crystal structure of a putative hd superfamily hydrolase (bh1327) from2 bacillus halodurans at 1.90 a resolution Phyre2 PDB 2rf9 chain D Region: 108 - 119 Aligned: 12 Modelled: 12 Confidence: 6.0% Identity: 50% PDB header:transferase Chain: D: PDB Molecule:erbb receptor feedback inhibitor 1; PDBTitle: crystal structure of the complex between the egfr kinase2 domain and a mig6 peptide Phyre2 PDB 3ccg chain A Region: 75 - 80 Aligned: 6 Modelled: 6 Confidence: 5.8% Identity: 33% PDB header:hydrolase Chain: A: PDB Molecule:hd superfamily hydrolase; PDBTitle: crystal structure of predicted hd superfamily hydrolase involved in2 nad metabolism (np_347894.1) from clostridium acetobutylicum at 1.503 a resolution Phyre2 PDB 1gng chain X Region: 67 - 76 Aligned: 10 Modelled: 10 Confidence: 5.7% Identity: 40% PDB header:transferase Chain: X: PDB Molecule:frattide; PDBTitle: glycogen synthase kinase-3 beta (gsk3) complex with frattide2 peptide Phyre2 PDB 1thz chain A Region: 112 - 121 Aligned: 10 Modelled: 10 Confidence: 5.6% Identity: 40% PDB header:transferase, hydrolase Chain: A: PDB Molecule:bifunctional purine biosynthesis protein purh; PDBTitle: crystal structure of avian aicar transformylase in complex2 with a novel inhibitor identified by virtual ligand3 screening Phyre2 1 d1rh5b_ 2 c2dyoB_ 3 c2l2tA_ 4 d2f22a1 5 c1bzgA_ 6 c1zczA_ 7 c3skdA_ 8 c2lkjA_ 9 d1lira_ 10 c2ogiA_ 11 c3zrkY_ 12 c2o08B_ 13 c2rf9D_ 14 c3ccgA_ 15 c1gngX_ 16 c1thzA_

Detailed template information

# Template Alignment Coverage 3D Model Confidence % i.d. Template Information

1 d1rh5b_ 12.1 15 Fold:Single transmembrane helix Superfamily:Preprotein translocase SecE subunit Family:Preprotein translocase SecE subunit 2 c2dyoB_ 11.2 40 PDB header:protein turnover/protein turnover Chain: B: PDB Molecule:autophagy protein 16; PDBTitle: the crystal structure of saccharomyces cerevisiae atg5-2 atg16(1-57) complex 3 c2l2tA_ 10.7 21 PDB header:membrane protein Chain: A: PDB Molecule:receptor tyrosine-protein kinase erbb-4; PDBTitle: solution nmr structure of the erbb4 dimeric membrane domain 4 d2f22a1 9.6 37 Fold:DinB/YfiT-like putative metalloenzymes Superfamily:DinB/YfiT-like putative metalloenzymes Family:DinB-like 5 c1bzgA_ 8.9 57 PDB header:hormone Chain: A: PDB Molecule:parathyroid hormone-related protein; PDBTitle: the solution structure of human parathyroid hormone-related2 protein (1-34) in near-physiological solution, nmr, 303 structures 6 c1zczA_ 8.8 50 PDB header:transferase/hydrolase Chain: A: PDB Molecule:bifunctional purine biosynthesis protein purh; PDBTitle: crystal structure of phosphoribosylaminoimidazolecarboxamide2 formyltransferase / imp cyclohydrolase (tm1249) from thermotoga3 maritima at 1.88 a resolution 7 c3skdA_ 8.6 50 PDB header:hydrolase Chain: A: PDB Molecule:putative uncharacterized protein tthb187; PDBTitle: crystal structure of the thermus thermophilus cas3 hd domain in the2 presence of ni2+ 8 c2lkjA_ 7.6 50 PDB header:cell adhesion Chain: A: PDB Molecule:integrin alpha-m; PDBTitle: structures and interaction analyses of the integrin alpha-m beta-22 cytoplasmic tails 9 d1lira_ 6.8 63 Fold:Knottins (small inhibitors, toxins, lectins) Superfamily:Scorpion toxin-like Family:Short-chain scorpion toxins 10 c2ogiA_ 6.7 50 PDB header:hydrolase Chain: A: PDB Molecule:hypothetical protein sag1661; PDBTitle: crystal structure of a putative metal dependent phosphohydrolase2 (sag1661) from streptococcus agalactiae serogroup v at 1.85 a3 resolution 11 c3zrkY_ 6.4 40 PDB header:transferase/peptide Chain: Y: PDB Molecule:proto-oncogene frat1; PDBTitle: identification of 2-(4-pyridyl)thienopyridinones as gsk-3beta2 inhibitors 12 c2o08B_ 6.3 33 PDB header:hydrolase Chain: B: PDB Molecule:bh1327 protein; PDBTitle: crystal structure of a putative hd superfamily hydrolase (bh1327) from2 bacillus halodurans at 1.90 a resolution 13 c2rf9D_ 6.0 50 PDB header:transferase Chain: D: PDB Molecule:erbb receptor feedback inhibitor 1; PDBTitle: crystal structure of the complex between the egfr kinase2 domain and a mig6 peptide 14 c3ccgA_ 5.8 33 PDB header:hydrolase Chain: A: PDB Molecule:hd superfamily hydrolase; PDBTitle: crystal structure of predicted hd superfamily hydrolase involved in2 nad metabolism (np_347894.1) from clostridium acetobutylicum at 1.503 a resolution 15 c1gngX_ 5.7 40 PDB header:transferase Chain: X: PDB Molecule:frattide; PDBTitle: glycogen synthase kinase-3 beta (gsk3) complex with frattide2 peptide 16 c1thzA_ 5.6 40 PDB header:transferase, hydrolase Chain: A: PDB Molecule:bifunctional purine biosynthesis protein purh; PDBTitle: crystal structure of avian aicar transformylase in complex2 with a novel inhibitor identified by virtual ligand3 screening

Binding site prediction

Due to computational demand, binding site predictions are not run for batch jobs

If you want to predict binding sites, please manually submit your model of choice to 3DLigandSite

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Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
If you use the binding site predictions from 3DLigandSite, please also cite:
3DLigandSite: predicting ligand-binding sites using similar structures.
Wass MN, Kelley LA and Sternberg MJ Nucleic Acids Research 38, W469-73 (2010) [PubMed]
© Structural Bioinformatics Group Imperial College London Lawrence Kelley, Benjamin Jefferys Disclaimer Terms and Conditions	Component software Template detection: HHpred 1.51 Secondary structure prediction: Psi-pred 2.5 Disorder prediction: Disopred 2.4 Transmembrane prediction: Memsat_SVM Multi-template modelling and ab initio: Poing 1.0