Return to main results Retrieve Phyre Job Id

Job DescriptionP0A8T7
Confidence100.00%DateWed Jan 25 15:20:19 GMT 2012
Rank3Aligned Residues1215
% Identity24%Templated1twfa_
SCOP infobeta and beta-prime subunits of DNA dependent RNA-polymerase beta and beta-prime subunits of DNA dependent RNA-polymerase RNA-polymerase beta-prime
Resolution2.30

  Insertion relative to template
  Deletion relative to template
  Catalytic residue from the CSA
 
Detailed help on interpreting your alignment


   910.........20.........30.........40.........50.........60.........70.........80........
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Query Sequence  KAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETINYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKC
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Template Sequence  YSSAPLRTVKEVQFGLFSPEEVRAISVAKIRFPETMDETQTRAKIGGLNDPRL. . . . . . . . . . . . . . . GSIDRNLKCQTC
Template Known Secondary structure 


S











T






S

S
TTS

S

SSSS
SSS...............


TT


SSS
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   6...10.........20.........30.........40.........50........ .60.........70
 
   8990.........100.........110.........120.........130.... .....140.........150.........160
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Query Sequence  GVEVTQTKVRRERMGHIELASPTAHIWFLKSLPSRIGLLLDMPLRD. . . . . . . . IERVLYFESYVVIEGGMTNLERQQIL
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Template Sequence  QEGMNECP. . . GHFGHIDLAKPVFHVGFIAKIKKVCECVCMHCGKLLLDEHNELMRQALAIKDSKKRFAAIWTLCKTKMV
Template Known Secondary structure 


TTS

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GGGB
TTT

BS

TTST

STT

B
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   71....... .80.........90.........100.........110.........120.........130.........140.......
 
   161........170.........180.........190.........200 .........210.........220........
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Query Sequence  TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQ. . . . . . . . . . . . LREELNETNSETKRKKLTKRIKLLEAFV
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Template Sequence  CETDV. . PSEDDPTQLVSRGGCGNTQPTIRKDGLKLVGSWKKDRATGDADEPELRVLSTEEILNIFKHISVKDFTSLGFN
Template Known Secondary structure 

S..

SS
TTS


S


B




SS

SSSS
TTS
S

TTS
TT
B
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   148.150.. .......160.........170.........180.........190.........200.........210.........220.....
 
   229230.........240.........250.........260.........270.........280.........290.........300........
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Query Sequence  QSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRNNRLKRLLDLAAPDIIVRNEKRMLQEAVDALLD
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Template Sequence  EVFSRPEWMILTCLPVPPPPVRPSISFNESQRGEDDLTFKLADILKANISLETLEHNGAPHHAIEEAESLLQFHVATYMD
Template Known Secondary structure  TTTB
GGGG



TTTS




SSS




SS



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   226...230.........240.........250.........260.........270.........280.........290.........300.....
 
   309310..... ....320.........330.........340.........350.........360.........370.........380.......
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Query Sequence  NGRRGRA. ITGSNKRPLKSLADMIKGKQGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLEL
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Template Sequence  NDIAGQPQALQKSGRPVKSIRARLKGKEGRIRGNLMGKRVDFSARTVISGDPNLELDQVGVPKSIAKTLTYPEVVTPYNI
Template Known Secondary structure 

SS




SS
TT




STTS
TTTTTTSSGGGTT
S

S
TTS
TTTT


TTT
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   306...310.........320.........330.........340.........350.........360.........370.........380.....
 
   388.390.........400....... ..410.........420.........430.........440....
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Query Sequence  RGLATTIKAAKKMVEREEAV. . . . . . . . . . . . . . . . . . . . . . . VWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEG
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Template Sequence  DRLTQLVRNGPNEHPGAKYVIRDSGDRIDLRYSKRAGDIQLQYGWKVERHIMDNDPVLFNRQPSLHKMSMMAHRVKVIPY
Template Known Secondary structure  TTTSSS

TT

TTS
TTTT




TT


TT

SS

SGGGSS
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   386...390.........400.........410.........420.........430.........440.........450.........460.....
 
   445....450.........460.........470.........480.........490.........500.........510.... .....520...
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Query Sequence  KAIQLHPLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVLGLYYMT. RDCVNAKGE
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Template Sequence  STFRLNLSVTSPYNADFDGDEMNLHVPQSEETRAELSQLCAVPLQIVSPQSNKPCMGIVQDTLCGIRKLTLRDTFIELDQ
Template Known Secondary structure  SS
GGGT

SSS



STBGGGGSTTTTS



STT
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   466...470.........480.........490.........500.........510.........520.........530.........540.....
 
   524.....530.........540.........550.........560.........570. ........580.........590.....
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Query Sequence  GMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAKTSLKD. . . . . . . . TTVGRAILWMIVPKGLPYSIVNQA
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Template Sequence  VLNMLY. . . . . . WVPDWDGVIP. . . TPAIIKPKPLWSGKQILSVAIPNGIHLQRFDEGTTLLSPKDNGMLIIDGQIIFGV
Template Known Secondary structure  ......STT

S


...

SB
SSS
S
TT



TT

TT
TT

STTS
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   546...550. ........560. ........570.........580.........590.........600.........610......
 
   596...600.. .......610.........620.........630.........640........ .650.........660.......
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Query Sequence  LGKKAIS. . . . KMLNTCYRILGLKPTVIFADQIMYTGFAYAARSGASVGIDDMVIPE. . . . KKHEIISEAEAEVAEIQEQ
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Template Sequence  VEKKTVGSSNGGLIHVVTREKGPQVCAKLFGNIQKVVNFWLLHNGFSTGIGDTIADGPTMREITETIAEAKKKVLDVTKE
Template Known Secondary structure 

SS
TTS
TT




GGGG


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   617..620.........630.........640.........650.........660.........670.........680.........690......
 
   668.670.........680.........690.........700.........710.........720.........730.........740.......
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Query Sequence  FQSGLVTAGERYNKVIDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQLAGMRGLM
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Template Sequence  AQANLLTAKHGMTLR. . . . . . . . ESFEDNVVRFLNEARDKAGRLAEVNLKDLNNVKQMVMAGSKGSFINIAQMSACVGQQ
Template Known Secondary structure  T




TT

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TT
TSS

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   697..700.........710. ........720.........730.........740.........750.........760........
 
   748.750........ .760.........770.........780.........790.........800.......
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Query Sequence  AKPDGSIIETP. . . . . . . . . . . . . . . . . . . . ITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTRRLVDVAQDL
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Template Sequence  SVEGKRIAFGFVDRTLPHFSKDDYSPESKGFVENSYLRGLTPQEFFFHAMGGREGLIDTAVKTAETGYIQRRLVKALEDI
Template Known Secondary structure 
BTTBS



BTTBSSTTS
TT

STTTTT
S
TTT


TT
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   769770.........780.........790.........800.........810.........820.........830.........840........
 
   808.810.........820.........830....
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Query Sequence  VVTEDDCGTHEGIMMTPVIEGGDVKEP. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
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Template Sequence  MVHYDNTTRNSLGNVIQFIYGEDGMDAAHIEKQSLDTIGGSDAAFEKRYRVDLLNTDHTLDPSLLESGSEILGDLKLQVL
Template Known Secondary structure 
TTS

TT

SSGGGTTB
GGG
GGGSS



TT
TTTS

TTTBTTTT
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   849850.........860.........870.........880.........890.........900.........910.........920........
 
   835....840.........850.........860..
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Query Sequence  . . . . LRDRVLGRVTAEDVLKPGTADILVPRNT. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
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Template Conservation                                     
                                            
Template Sequence  LDEEYKQLVKDRKFLREVFVDGEANWPLPVNIRRIIQNAQQTFHIDHTKPSDLTIKDIVLGVKDLQENLLVLRGKNEIIQ
Template Known Secondary structure  TTT


S
TT

TTS
B


TT

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S
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   929930.........940.........950.........960.........970.........980.........990.........1000........
 
   863......870.........880.........890.........900.........910.........920.........930.......
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Query Sequence  . . . . . LLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEAIGVIAAQSIGEPGTQLTMRTFHI
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Template Sequence  NAQRDAVTLFCCLLRSRLATRRVLQEYRLTKQAFDWVLSNIEAQ. . FLRSVVHPGEMVGVLAAQSIGEPATQMTLKKVT.
Template Known Secondary structure  TTS
TS


..B

TT

TT




S
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   10091010.........1020.........1030.........1040.........1050.. .......1060.........1070.........1080.....
 
   938.940.........950.........960.........970.........980.........990.........1000.........1010.......
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Query Sequence  GGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGKLVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETV
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Template Sequence  . . . . . . . . . . . . . . . . . . . . SGVPRLKEILNVAKNMKTPSLTVYLE. . PGHAADQEQAKLIRSAIEHTTLKSVTIASEIY
Template Known Secondary structure  ....................
TTT
SS
SS
B
..SSSSS
S
GGGT
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   1096...1100.........1110.........1120. ........1130.........1140.........1150...
 
   1018.1020.........1030.........1040.........1050.........1060.........1070.........1080.........1090.......
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Query Sequence  ANWDPHTMPVITEVSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDVLIPGTDMPA
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Template Sequence  YDPDPRSTVIPEDEEIIQLHFSL. . . . . . . . . . QQSPWLLRLELDRAAMNDKDLTMGQVGERIKQTFKNDLFVIWSEDND
Template Known Secondary structure 


SSS
SSGGGTSS

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S
TT

GGG

TTS
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   1154.....1160.........1170...... ...1180.........1190.........1200.........1210.........1220...
 
   1098.1100.........1110.........1120.........1130.........1140.........1150.........1160.........1170.......
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Query Sequence  QYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFEARRPKEPAILAEISGIVSFGKETKGKRRLVI
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Template Sequence  EKLI. . . . . . . . IRCRVVAEEDHMLKKIENTMLENITLRGVENIERVVMMK. . . . . . . . . . . . . . . . . . . . . . . YDRKVP
Template Known Secondary structure  SS
........


TT
S
TT


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   1234... ..1240.........1250.........1260.........1270...... ...1280..
 
   1178.1180.........1190.........1200.........1210.........1220.........1230.........1240.........1250.......
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Query Sequence  TPVDGSDPYEEMIPKWRQLNVFEGERVERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIV
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Template Sequence  SPTGEYVKEPEWVLETDGVNLSEVMTVPGIDPTRIYTNSFIDIMEVLGIEAGRAALYKEVYNVIASDGSYVNYRHMALLV
Template Known Secondary structure 
TTSSS

TTSTTB
TTT
B
S

TTT



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   1293......1300.........1310.........1320.........1330.........1340.........1350.........1360.........1370..
 
   1258.1260.........1270.........1280.........1290.........1300.........1310.... .....1320.........1330.....
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Query Sequence  RQMLRKATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASL. . ATESFISAASFQETTRVLTEA
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Template Sequence  DVMTTQGGL. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . TSVTRHGFNRSNTGALMRCSFEETVEILFEA
Template Known Secondary structure  TTTTSS
........................................

SSS
SSS
SS

TTSS

SS
S
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   1373......1380. ........1390.........1400.........1410..
 
   1336...1340.........1350.........1360......
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Query Sequence  AVAGKRDELRGLKENVIVGRLIPAGTGYAYH
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Template Sequence  GASAELDDCRGVSENVILGQMAPIGTGAFDV
Template Known Secondary structure  T



STT


SSGGGSS

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   1413......1420.........1430.........1440...
 
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Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
 
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Lawrence Kelley, Benjamin Jefferys 
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