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Job DescriptionP0A8T7
Confidence100.00%DateWed Jan 25 15:20:19 GMT 2012
Rank4Aligned Residues1159
% Identity52%Templated1smyd_
SCOP infobeta and beta-prime subunits of DNA dependent RNA-polymerase beta and beta-prime subunits of DNA dependent RNA-polymerase RNA-polymerase beta-prime
Resolution2.70

  Insertion relative to template
  Deletion relative to template
  Catalytic residue from the CSA
 
Detailed help on interpreting your alignment


   15....20.........30.........40.........50.........60.........70.........80.........90....
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Query Sequence  EEFDAIKIALASPDMIRSWSFGEVKKPETINYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQ
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Template Sequence  KEVRKVRIALASPEKIRSWSYGEVEKPETINYRTLKPERDGLFDERIFGPIKDYECACGKYKRQRFEGKVCERCGVEVTK
Template Known Secondary structure 










S


B
TTSSSB
SSSSS


SSSSS
SS
S

SSSSSS


SSS



SS
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   3......10.........20.........30.........40.........50.........60.........70.........80..
 
   95....100.........110.........120.........130.........140.........150.........160.........170....
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Query Sequence  TKVRRERMGHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQILTEEQYLDALEEFGD
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Template Sequence  SIVRRYRMGHIELATPAAHIWFVKDVPSKIGTLLDLSATELEQVLYFSKYIVLDPKGAILNGVPVEKRQLLTDEEYRELR
Template Known Secondary structure  T

GGGTSSSS
TTS
TTTS



S


S
SSSS

TT
B

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   83......90.........100.........110.........120.........130.........140.........150.........160..
 
   175
Predicted Secondary structure  ...............................................................................
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Query Sequence  E. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Query Conservation   ...............................................................................
Alig confidence 
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Template Conservation                                                                                  
Template Sequence  YGKQETYPLPPGVDALVKDGEEVVKGQELAPGVVSRLDGVALYRFPRRVRVEYVKKERAGLRLPLAAWVEKEAYKPGEIL
Template Known Secondary structure  T
S




STTTTGGGTBS



TT

SSS


S
S




SS


S

TTTTTTTGGGTSSS
TT
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   163......170.........180.........190.........200.........210.........220.........230.........240..
 
  
Predicted Secondary structure  ................................................................................
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Query Sequence  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Query Conservation  ................................................................................
Template Conservation   
                                                                              
Template Sequence  AELPEPYLFGDKIVAAIDPEEEVIAEAEGVVHLHEPASILVVKARVYPFEDDVEVSTGDRVAPGDVLADGGKVKSDVYGR
Template Known Secondary structure  TTSTTTT






SS


SS

BTTB
S







SSS



TTSS

S

SSS


S

SS



SS

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   243......250.........260.........270.........280.........290.........300.........310.........320..
 
   176...180.........190.........200.........210.........220.........230........
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Query Sequence  . . . . . . . . . . . . . . . . . FDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLTKRIKLLEAFVQSGNKPEWMI
Query Conservation  .................                                         
         
      
   
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Template Conservation                            
  

    
          
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Template Sequence  VEVDLVRNVVRVVESYDIDARMGAEAIQQLLKELDLEALEKELLEEMK. HPSRARRAKARKRLEVVRAFLDSGNRPEWMI
Template Known Secondary structure  TTTT






SS

.SS
TTS

GGGG
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   435....440.........450.........460.........470.........480.. .......490.........500.........510...
 
   239240.........250.........260.........270.........280.........290.........300.........310..... ..
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.

Query Sequence  LTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRNNRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRA. IT
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Template Conservation 
  




   

            


  
  

  
  
                   

  
    

         
Template Sequence  LEAVPVLPPDLRPMVQVDGGRFATSDLNDLYRRLINRNNRLKKLLAQGAPEIIIRNEKRMLQEAVDALLDNGRRGAPVTN
Template Known Secondary structure 

STTS

STT

TT



TTSS


B
S
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   514.....520.........530.........540.........550.........560.........570.........580.........590...
 
   318.320.........330.........340.........350.........360.........370.........380.... .....390
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.......
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Query Sequence  GSNKRPLKSLADMIKGKQGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGK. . . . . . . LELRGL
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Template Conservation         
 
 
 



 



 

 






 







 
   




   
  

 
  
  
           
Template Sequence  PGSDRPLRSLTDILSGKQGRFRQNLLGKRVDYSGRSVIVVGPQLKLHQCGLPKRMALELFKPFLLKKMEEKGIAPNVKAA
Template Known Secondary structure  TT


B


TTTTTT



SS
TT

TTB
BTSTT
SS
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   594.....600.........610.........620.........630.........640.........650.........660.........670...
 
   391........400.........410.........420.........430.........440.........450.........460.........470
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Query Sequence  ATTIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLHPLVCAAYNADFDGDQMAVHV
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Template Conservation     
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Template Sequence  RRMLERQRDIKDE. . . . VWDALEEVIHGKVVLLNRAPTLHRLGIQAFQPVLVEGQSIQLHPLVCEAFNADFDGDQMAVHV
Template Known Secondary structure  SGGG

T....SS


SS

SGGG

BSSSS
SGGGTTTT

SSS

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   674.....680...... ...690.........700.........710.........720.........730.........740.........
 
   471........480.........490.........500.........510.... .....520.........530.........540.........
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Query Sequence  PLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVLGLYYMT. RDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVK
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Template Conservation 


  




  

       

  
 

  
 

 
 
   

    
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Template Sequence  PLSSFAQAEARIQMLSAHNLLSPASGEPLAKPSRDIILGLYYITQVRKEKKGAGLEFATPEE. ALAAHERGEVALN. . . A
Template Known Secondary structure 

SSBSTT

B
SSSSSBSS


SS







S


TTS
S.TTS...S
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.








...
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   750.........760.........770.........780.........790.........800.........810. ........820.... .
 
   550.........560.........570 .........580 .........590.....
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Query Sequence  VRITEYEKDANGELVAKTSLK. . . . . . . . . . . . . . . . . . . . . . . . . . . DTTVGRAILW. . . . . . . MIVPKGLPYSIVNQA
Query Conservation             
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Template Conservation 
 
 
   





     
                                
                           
Template Sequence  PIKVAGRETSVGRLKYVFANPDEALLAVAHGIVDLQDVVTVRYMGKRLETSPGRILFARIVAEAVEDEKVAWELIQLDVP
Template Known Secondary structure 
SS


SS


SSTTSS
TTS

TT


SS
S

TTS
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   826...830.........840.........850.........860.........870.........880.........890.........900.....
 
   596...600.........610.........620.........630.........640.........650.........660.........670.....
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Query Sequence  LGKKAISKMLNTCYRILGLKPTVIFADQIMYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTA
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Template Sequence  QEKNSLKDLVYQAFLRLGMEKTARLLDALKYYGFTFSTTSGITIGIDDAVIPEEKKQYLEEADRKLLQIEQAYEMGFLTD
Template Known Secondary structure 

T
T



GGGS


TT
S
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   906...910.........920.........930.........940.........950.........960.........970.........980.....
 
   676...680.........690.........700.........710.........720.........730.........740.........750.....
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Query Sequence  GERYNKVIDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQLAGMRGLMAKPDGSII
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Template Conservation        
   
              
 
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Template Sequence  RERYDQILQLWTETTEKVTQAVFKNFEEN. . . . . . . . . . . . YPFNPLYVMAQSGARGNPQQIRQLCGLRGLMQKPSGETF
Template Known Secondary structure  T............
TT
SSSS

TT




B
SSS
B
Template Predicted Secondary structure 



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   986...990.........1000.........1010.... .....1020.........1030.........1040.........1050...
 
   756...760.........770.........780.........790.........800.........810.........820.........830.....
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Query Sequence  ETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTRRLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPL
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Template Sequence  EVPVRSSFREGLTVLEYFISSHGARKGGADTALRTADSGYLTRKLVDVTHEIVVREA. DCGTTNYISVPLFQPDEVTRSL
Template Known Secondary structure  SS




SSS


S
STTTT
B


S.

S







TTSSS

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   1054.....1060.........1070.........1080.........1090.........1100.........1110 .........1120.........1130..
 
   836...840.........850.........860. ........870.........880.........890.........900......
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Query Sequence  RDRVLGRVTAEDVLKPGTADILVPRN. . . . . . . . . TLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARG
Query Conservation 
     
 
   
        
   
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Template Conservation                
                            
         
 


 
       
    
   
   
Template Sequence  RLRKRADIEAGLYGRVLAREVEVLGVRLEEGRYLSMDDVHLLIKAAEAGEIQEVPVRSPLTCQTRYGVCQKCYGYDLSMA
Template Known Secondary structure 

TT
BBSS

SS

TT
B

SS
S



GGG
SSTTB


S
SSSS
Template Predicted Secondary structure 




















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   1133......1140.........1150.........1160.........1170.........1180.........1190.........1200.........1210..
 
   907..910.........920.........930.........940 .........950.........960.........970.........980.....
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Query Sequence  HIINKGEAIGVIAAQSIGEPGTQLTMRTFHIGGA. ASRAAAESSIQVKNKGSIKLSNVKSVVNSSGKLVITSRNTELKLI
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Template Sequence  RPVSIGEAVGIVAAQSIGEPGTQLTMRTFHTGGVAGAADI. . . . . . . . . . . TQGLPRVIELFEARRPKAKAVIS. . . . . .
Template Known Secondary structure  S


SS

TTS

S
TT
S
SSTT


...........

TT


TTB

B
......
Template Predicted Secondary structure 















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......
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   1213......1220.........1230.........1240.........1250.. .......1260.........1270.....
 
   986...990.........1000.........1010.........1020.........1030.........1040.........1050.........1060.....
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Query Sequence  DEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITEVSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSA
Query Conservation                  
        
               
                                      
Template Conservation  ................................................................................
Template Sequence  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Template Known Secondary structure  ................................................................................
Template Predicted Secondary structure  ................................................................................
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   1066...1070.........1080.........1090.........1100.........1110.........1120.........1130.........1140.....
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Query Sequence  ERTAGGKDLRPALKIVDAQGNDVLIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLF
Query Conservation                                                                      
  
   
    
Template Conservation  ................................................................................
Template Sequence  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Template Known Secondary structure  ................................................................................
Template Predicted Secondary structure  ................................................................................
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   1146...1150.........1160.........1170.........1180.........1190.........1200.........1210.........1220.....
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Query Sequence  EARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERVERGDVISDGPEAPHDILRLRG
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Template Conservation  ............     
          ...             
     
 
     
               

   
Template Sequence  . . . . . . . . . . . . EIDGVVRIEETEEKLS. . . VFVESEGFSKEYKLPKEARLLVKDGDYVEAGQPLTRGAIDPHQLLEAKG
Template Known Secondary structure  ............SS



SS


...
SS



TT





TT

TT
B
SSSB


Template Predicted Secondary structure  ............






...





















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   1276...1280.........1290. ........1300.........1310.........1320.........1330.........1340
 
   1226...1230.........1240.........1250.........1260.........1270.........1280.........1290.........1300.....
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Query Sequence  VHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLRKATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRD
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Template Conservation         

 
                
  
          


       





  
  
        
        
Template Sequence  PEAVERYLVEEIQKVYRAQGVKLHDKHIEIVVRQMMKYVEVTDPGDSRLLEGQVLEKWDVEALNERLIAEGKTPVAWKPL
Template Known Secondary structure  TTT



T
SS
SSS
SS

TS
S





Template Predicted Secondary structure 


























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   1341........1350.........1360.........1370.........1380.........1390.........1400.........1410.........1420
 
   1306...1310.........1320.........1330.........1340.........1350.........1360....... ..1370.........1380...
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Query Sequence  LLGITKASLATESFISAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQ. . DRMRRRAAGEAPAAPQ
Query Conservation 





 

   


  



 
   
  

  
  
 
 


  

 

    


 
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Template Sequence  LMGVTKSALSTKSWLSAASFQNTTHVLTEAAIAGKKDELIGLKENVILGRLIPAGTGSDFVRFTQVVDQKTLKAIEEARK
Template Known Secondary structure 


T
S
TTSTTT


STT


SSSTT
TTTSS




Template Predicted Secondary structure 































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   1421........1430.........1440.........1450.........1460.........1470.........1480.........1490.........1500
 
   1384...
Predicted Secondary structure 



Query SS confidence 



Query Sequence  VTAE
Query Conservation      
Alig confidence 



Template Conservation      
Template Sequence  EAVE
Template Known Secondary structure  TTS
Template Predicted Secondary structure 



Template SS confidence 



   1501...
 
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Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
 
© Structural Bioinformatics Group, Imperial College, London
Lawrence Kelley, Benjamin Jefferys 
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