Secondary structure and disorder prediction

		1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	N	K	Q	S	W	L	L	N	L	S	L	L	K	T	H	P	A	F	R	A	V	F	L	A	R	F	I	S	I	V	S	L	G	L	L	G	V	A	V	P	V	Q	I	Q	M	M	T	H	S	T	W	Q	V	G	L	S	V	T	L
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		T	G	G	A	M	F	V	G	L	M	V	G	G	V	L	A	D	R	Y	E	R	K	K	V	I	L	L	A	R	G	T	C	G	I	G	F	I	G	L	C	L	N	A	L	L	P	E	P	S	L	L	A	I	Y	L	L	G	L	W	D
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		G	F	F	A	S	L	G	V	T	A	L	L	A	A	T	P	A	L	V	G	R	E	N	L	M	Q	A	G	A	I	T	M	L	T	V	R	L	G	S	V	I	S	P	M	I	G	G	L	L	L	A	T	G	G	V	A	W	N	Y	G
Secondary structure
SS confidence
Disorder																		?			?
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		L	A	A	A	G	T	F	I	T	L	L	P	L	L	S	L	P	A	L	P	P	P	P	Q	P	R	E	H	P	L	K	S	L	L	A	G	F	R	F	L	L	A	S	P	L	V	G	G	I	A	L	L	G	G	L	L	T	M	A	S
Secondary structure
SS confidence
Disorder																		?	?	?	?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300
Sequence		A	V	R	V	L	Y	P	A	L	A	D	N	W	Q	M	S	A	A	Q	I	G	F	L	Y	A	A	I	P	L	G	A	A	I	G	A	L	T	S	G	K	L	A	H	S	A	R	P	G	L	L	M	L	L	S	T	L	G	S	F	L
Secondary structure
SS confidence
Disorder																																									?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	310	.	.	.	.	.	.	.	.	.	320	.	.	.	.	.	.	.	.	.	330	.	.	.	.	.	.	.	.	.	340	.	.	.	.	.	.	.	.	.	350	.	.	.	.	.	.	.	.	.	360
Sequence		A	I	G	L	F	G	L	M	P	M	W	I	L	G	V	V	C	L	A	L	F	G	W	L	S	A	V	S	S	L	L	Q	Y	T	M	L	Q	T	Q	T	P	E	A	M	L	G	R	I	N	G	L	W	T	A	Q	N	V	T	G	D
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	370	.	.	.	.	.	.	.	.	.	380	.	.	.	.	.	.	.	.	.	390	.	.	.	.	.	.	.	.	.	400	.	.	.	.	.	.	.	.	.	410	.	.	.	.	.	.
Sequence		A	I	G	A	A	L	L	G	G	L	G	A	M	M	T	P	V	A	S	A	S	A	S	G	F	G	L	L	I	I	G	V	L	L	L	L	V	L	V	E	L	R	H	F	R	Q	T	P	P	Q	V	T	A	S	D	S
Secondary structure
SS confidence
Disorder																																												?		?	?	?	?	?	?	?	?	?	?	?	?
Disorder confidence

Confidence Key
High(9)											Low (0)

?	Disordered
	Alpha helix
	Beta strand

Domain analysis

Hover over an aligned region to see model and summary info

Please note, only up to the top 20 hits are modelled to reduce computer load

Rank Aligned region

PDB 1pv7 chain A Region: 15 - 413 Aligned: 399 Modelled: 399 Confidence: 100.0% Identity: 10% Fold: MFS general substrate transporter Superfamily: MFS general substrate transporter Family: LacY-like proton/sugar symporter Phyre2 PDB 1pw4 chain A Region: 3 - 410 Aligned: 406 Modelled: 408 Confidence: 100.0% Identity: 12% Fold: MFS general substrate transporter Superfamily: MFS general substrate transporter Family: Glycerol-3-phosphate transporter Phyre2 PDB 2gfp chain A Region: 21 - 390 Aligned: 364 Modelled: 370 Confidence: 100.0% Identity: 14% PDB header:membrane protein Chain: A: PDB Molecule:multidrug resistance protein d; PDBTitle: structure of the multidrug transporter emrd from2 escherichia coli Phyre2 PDB 3o7p chain A Region: 17 - 401 Aligned: 381 Modelled: 385 Confidence: 100.0% Identity: 10% PDB header:transport protein Chain: A: PDB Molecule:l-fucose-proton symporter; PDBTitle: crystal structure of the e.coli fucose:proton symporter, fucp (n162a) Phyre2 PDB 2xut chain C Region: 22 - 397 Aligned: 372 Modelled: 376 Confidence: 99.9% Identity: 12% PDB header:transport protein Chain: C: PDB Molecule:proton/peptide symporter family protein; PDBTitle: crystal structure of a proton dependent oligopeptide (pot)2 family transporter. Phyre2 PDB 2jo1 chain A Region: 371 - 415 Aligned: 45 Modelled: 45 Confidence: 16.0% Identity: 16% PDB header:hydrolase regulator Chain: A: PDB Molecule:phospholemman; PDBTitle: structure of the na,k-atpase regulatory protein fxyd1 in2 micelles Phyre2 PDB 2g9p chain A Region: 71 - 84 Aligned: 14 Modelled: 14 Confidence: 13.7% Identity: 29% PDB header:antimicrobial protein Chain: A: PDB Molecule:antimicrobial peptide latarcin 2a; PDBTitle: nmr structure of a novel antimicrobial peptide, latarcin 2a,2 from spider (lachesana tarabaevi) venom Phyre2 PDB 3hd6 chain A Region: 25 - 219 Aligned: 195 Modelled: 195 Confidence: 8.0% Identity: 10% PDB header:membrane protein, transport protein Chain: A: PDB Molecule:ammonium transporter rh type c; PDBTitle: crystal structure of the human rhesus glycoprotein rhcg Phyre2 PDB 3hur chain A Region: 1 - 16 Aligned: 16 Modelled: 16 Confidence: 7.2% Identity: 13% PDB header:isomerase Chain: A: PDB Molecule:alanine racemase; PDBTitle: crystal structure of alanine racemase from oenococcus oeni Phyre2 PDB 2knc chain A Region: 376 - 416 Aligned: 41 Modelled: 41 Confidence: 6.7% Identity: 15% PDB header:cell adhesion Chain: A: PDB Molecule:integrin alpha-iib; PDBTitle: platelet integrin alfaiib-beta3 transmembrane-cytoplasmic2 heterocomplex Phyre2 PDB 3b9y chain A Region: 34 - 223 Aligned: 190 Modelled: 190 Confidence: 6.5% Identity: 9% PDB header:transport protein Chain: A: PDB Molecule:ammonium transporter family rh-like protein; PDBTitle: crystal structure of the nitrosomonas europaea rh protein Phyre2 PDB 2jp3 chain A Region: 371 - 415 Aligned: 45 Modelled: 45 Confidence: 6.4% Identity: 18% PDB header:transcription Chain: A: PDB Molecule:fxyd domain-containing ion transport regulator 4; PDBTitle: solution structure of the human fxyd4 (chif) protein in sds2 micelles Phyre2 PDB 1rh1 chain A domain 2 Region: 9 - 81 Aligned: 69 Modelled: 73 Confidence: 6.2% Identity: 17% Fold: Toxins' membrane translocation domains Superfamily: Colicin Family: Colicin Phyre2 PDB 1xl6 chain B Region: 1 - 102 Aligned: 102 Modelled: 102 Confidence: 5.4% Identity: 8% PDB header:metal transport Chain: B: PDB Molecule:inward rectifier potassium channel; PDBTitle: intermediate gating structure 2 of the inwardly rectifying k+ channel2 kirbac3.1 Phyre2 1 d1pv7a_ 2 d1pw4a_ 3 c2gfpA_ 4 c3o7pA_ 5 c2xutC_ 6 c2jo1A_ 7 c2g9pA_ 8 c3hd6A_ 9 c3hurA_ 10 c2kncA_ 11 c3b9yA_ 12 c2jp3A_ 13 d1rh1a2 14 c1xl6B_

Detailed template information

# Template Alignment Coverage 3D Model Confidence % i.d. Template Information

1 d1pv7a_ 100.0 10 Fold:MFS general substrate transporter Superfamily:MFS general substrate transporter Family:LacY-like proton/sugar symporter 2 d1pw4a_ 100.0 12 Fold:MFS general substrate transporter Superfamily:MFS general substrate transporter Family:Glycerol-3-phosphate transporter 3 c2gfpA_ 100.0 14 PDB header:membrane protein Chain: A: PDB Molecule:multidrug resistance protein d; PDBTitle: structure of the multidrug transporter emrd from2 escherichia coli 4 c3o7pA_ 100.0 10 PDB header:transport protein Chain: A: PDB Molecule:l-fucose-proton symporter; PDBTitle: crystal structure of the e.coli fucose:proton symporter, fucp (n162a) 5 c2xutC_ 99.9 12 PDB header:transport protein Chain: C: PDB Molecule:proton/peptide symporter family protein; PDBTitle: crystal structure of a proton dependent oligopeptide (pot)2 family transporter. 6 c2jo1A_ 16.0 16 PDB header:hydrolase regulator Chain: A: PDB Molecule:phospholemman; PDBTitle: structure of the na,k-atpase regulatory protein fxyd1 in2 micelles 7 c2g9pA_ 13.7 29 PDB header:antimicrobial protein Chain: A: PDB Molecule:antimicrobial peptide latarcin 2a; PDBTitle: nmr structure of a novel antimicrobial peptide, latarcin 2a,2 from spider (lachesana tarabaevi) venom 8 c3hd6A_ 8.0 10 PDB header:membrane protein, transport protein Chain: A: PDB Molecule:ammonium transporter rh type c; PDBTitle: crystal structure of the human rhesus glycoprotein rhcg 9 c3hurA_ 7.2 13 PDB header:isomerase Chain: A: PDB Molecule:alanine racemase; PDBTitle: crystal structure of alanine racemase from oenococcus oeni 10 c2kncA_ 6.7 15 PDB header:cell adhesion Chain: A: PDB Molecule:integrin alpha-iib; PDBTitle: platelet integrin alfaiib-beta3 transmembrane-cytoplasmic2 heterocomplex 11 c3b9yA_ 6.5 9 PDB header:transport protein Chain: A: PDB Molecule:ammonium transporter family rh-like protein; PDBTitle: crystal structure of the nitrosomonas europaea rh protein 12 c2jp3A_ 6.4 18 PDB header:transcription Chain: A: PDB Molecule:fxyd domain-containing ion transport regulator 4; PDBTitle: solution structure of the human fxyd4 (chif) protein in sds2 micelles 13 d1rh1a2 6.2 17 Fold:Toxins' membrane translocation domains Superfamily:Colicin Family:Colicin 14 c1xl6B_ 5.4 8 PDB header:metal transport Chain: B: PDB Molecule:inward rectifier potassium channel; PDBTitle: intermediate gating structure 2 of the inwardly rectifying k+ channel2 kirbac3.1

Binding site prediction

Due to computational demand, binding site predictions are not run for batch jobs

If you want to predict binding sites, please manually submit your model of choice to 3DLigandSite

Transmembrane helix prediction

Transmembrane helices have been predicted in your sequence to adopt the topology shown below

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Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
If you use the binding site predictions from 3DLigandSite, please also cite:
3DLigandSite: predicting ligand-binding sites using similar structures.
Wass MN, Kelley LA and Sternberg MJ Nucleic Acids Research 38, W469-73 (2010) [PubMed]
© Structural Bioinformatics Group Imperial College London Lawrence Kelley, Benjamin Jefferys Disclaimer Terms and Conditions	Component software Template detection: HHpred 1.51 Secondary structure prediction: Psi-pred 2.5 Disorder prediction: Disopred 2.4 Transmembrane prediction: Memsat_SVM Multi-template modelling and ab initio: Poing 1.0