Job Description P39383 Confidence 99.50% Date Thu Jan 5 12:00:15 GMT 2012 Rank 40 Aligned Residues 238 % Identity 13% Template c3ezwD_ PDB info PDB header:transferase Chain: D: PDB Molecule:glycerol kinase; PDBTitle: crystal structure of a hyperactive escherichia coli glycerol kinase2 mutant gly230 --> asp obtained using microfluidic crystallization3 devices Resolution 2.00 Å

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		2	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20		.	.	.	.	.	.	.	.	.	30	.	.	.	.																		.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60	.	.
Predicted Secondary structure																					.															.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.																													.
Query SS confidence																					.															.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.																													.
Query Sequence		A	Y	S	I	G	I	D	S	G	S	T	A	T	K	G	I	L	L	A	.	D	G	V	I	T	R	R	F	L	V	P	T	P	F	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	R	P	A	T	A	I	T	E	A	W	E	T	L	R	E	G	L	E	T	T	P	F	L	T	L	T	G	Y	.
Query Conservation																					.															.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.																													.
Alig confidence																					.															.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.																													.
Template Conservation
Template Sequence		K	Y	I	V	A	L	D	Q	G	T	T	S	S	R	A	V	V	M	D	H	D	A	N	I	I	S	V	S	Q	R	E	F	E	Q	I	Y	P	K	P	G	W	V	E	H	D	P	M	E	I	W	A	T	Q	S	S	T	L	V	E	V	L	A	K	A	D	I	S	S	D	Q	I	A	A	I	G	I	T	N	Q	R
Template Known Secondary structure											S	S									T	T														B			S	T	T																								T	T			G	G	G										S
Template Predicted Secondary structure
Template SS confidence
		4	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60	.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.
																																							63	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.
Predicted Secondary structure		.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.																																.	.	.	.	.	.	.	.	.	.	.	.
Query SS confidence		.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.																																.	.	.	.	.	.	.	.	.	.	.	.
Query Sequence		.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	G	R	Q	L	V	D	F	A	D	K	Q	V	T	E	I	S	C	H	G	L	G	A	R	F	L	A	P	A	T	R	A	.	.	.	.	.	.	.	.	.	.	.	.
Query Conservation		.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.																																.	.	.	.	.	.	.	.	.	.	.	.
Alig confidence		.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.																																.	.	.	.	.	.	.	.	.	.	.	.
Template Conservation
Template Sequence		E	T	T	I	V	W	E	K	E	T	G	K	P	I	Y	N	A	I	V	W	Q	C	R	R	T	A	E	I	C	E	H	L	K	R	D	G	L	E	D	Y	I	R	S	N	T	G	L	V	I	D	P	Y	F	S	G	T	K	V	K	W	I	L	D	H	V	E	G	S	R	E	R	A	R	R	G	E	L	L	F	G
Template Known Secondary structure		S		B					T	T	T			B	S	S					T	T			T	T										T	T										S			S	S	T	T	S												S	T	T							T	T	S
Template Predicted Secondary structure
Template SS confidence
		84	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120	.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.
Predicted Secondary structure		.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.
Query SS confidence		.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.
Query Sequence		.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.
Query Conservation		.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.
Template Conservation
Template Sequence		T	V	D	T	W	L	I	W	K	M	T	Q	G	R	V	H	V	T	D	Y	T	N	A	S	R	T	M	L	F	N	I	H	T	L	D	W	D	D	K	M	L	E	V	L	D	I	P	R	E	M	L	P	E	V	R	R	S	S	E	V	Y	G	Q	T	N	R	I	P	I	S	G	I	A	G	D	Q	Q	A	A	L
Template Known Secondary structure												T	T	T	S										G	G	G	S	S			T	T	T	T										T	T			G	G	G							S
Template Predicted Secondary structure
Template SS confidence
		164	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180	.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240	.	.	.
												94	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.																	.	.	.	.	120	.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.
Predicted Secondary structure		.	.	.	.	.	.	.	.	.	.																							.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.
Query SS confidence		.	.	.	.	.	.	.	.	.	.																							.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.
Query Sequence		.	.	.	.	.	.	.	.	.	.	V	I	D	I	G	G	Q	D	S	K	V	I	Q	L	D	D	D	G	N	L	C	D	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	F	L	M	N	D	K	C	A	A	G	T	G	R	F	L	E	V	I	S	R	T	L	G	T	S	V	E	Q	L	D	S	I
Query Conservation		.	.	.	.	.	.	.	.	.	.																							.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.
Alig confidence		.	.	.	.	.	.	.	.	.	.																							.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.
Template Conservation
Template Sequence		F	G	Q	L	C	V	K	E	G	M	A	K	N	T	Y	G	T	G	C	F	M	L	M	N	T	G	E	K	A	V	K	S	E	N	G	L	L	T	T	I	A	C	G	P	T	G	E	V	N	Y	A	L	E	G	A	V	F	M	A	G	A	S	I	Q	W	L	R	D	E	M	K	L	I	D	S	E	Y	F	A	T
Template Known Secondary structure				T	T			S	T	T							S	S	S								T	T	S					S	S	S									T	T	S											S		S										T	S
Template Predicted Secondary structure
Template SS confidence
		251	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300	.	.	.	.	.	.	.	.	.	310	.	.	.	.	.	.	.	.	.	320	.	.	.	.	.	.	.	.	.	330
		148	.	150	.	.															.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180	.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200						.	.	.	.	.	.	.	.
Predicted Secondary structure							.	.	.	.	.	.	.	.	.	.	.	.	.	.																																																	.	.	.	.	.
Query SS confidence							.	.	.	.	.	.	.	.	.	.	.	.	.	.																																																	.	.	.	.	.
Query Sequence		T	E	N	V	T	.	.	.	.	.	.	.	.	.	.	.	.	.	.	P	H	A	I	T	S	M	C	T	V	F	A	E	S	E	A	I	S	L	R	S	A	G	V	A	P	E	A	I	L	A	G	V	I	N	A	M	A	R	R	S	A	N	F	I	A	R	L	.	.	.	.	.	S	C	E	A	P	I	L	F
Query Conservation							.	.	.	.	.	.	.	.	.	.	.	.	.	.																																																	.	.	.	.	.
Alig confidence							.	.	.	.	.	.	.	.	.	.	.	.	.	.														.	.	.	.	.	.																														.	.	.	.	.
Template Conservation																																		.	.	.	.	.	.
Template Sequence		K	V	Q	N	T	N	G	V	Y	V	V	P	A	F	T	G	L	G	A	P	Y	W	D	P	Y	A	R	G	A	I	F	G	.	.	.	.	.	.	L	T	R	G	V	N	A	N	H	I	I	R	A	T	L	E	S	I	A	Y	Q	T	R	D	V	L	E	A	M	Q	A	D	S	G	I	R	L	H	A	L	R	V
Template Known Secondary structure		T	S	S	S		T	T							T	T		B		T	T	T		B	T	T								.	.	.	.	.	.			T	T																																		S
Template Predicted Secondary structure																																		.	.	.	.	.	.
Template SS confidence
		335	.	.	.	.	340	.	.	.	.	.	.	.	.	.	350	.	.	.	.	.	.	.	.	.	360	.	.	.	.	.	.							.	.	.	370	.	.	.	.	.	.	.	.	.	380	.	.	.	.	.	.	.	.	.	390	.	.	.	.	.	.	.	.	.	400	.	.	.	.	.	.	.	.
		209	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240	.	.	.	.	.	.	.	.	.
Predicted Secondary structure
Query SS confidence
Query Sequence		T	G	G	V	S	H	C	Q	K	F	A	R	M	L	E	S	H	L	R	M	P	V	N	T	H	P	D	A	Q	F	A	G	A	I	G	A	A	V	I	G	Q
Query Conservation
Alig confidence																												.	.	.	.
Template Conservation																												.	.	.	.
Template Sequence		D	G	G	A	V	A	N	N	F	L	M	Q	F	Q	S	D	I	L	G	T	R	V	E	R	P	E	.	.	.	.	V	T	A	L	G	A	A	Y	L	A	G
Template Known Secondary structure			S	G	G	G	G													T	S							.	.	.	.
Template Predicted Secondary structure																												.	.	.	.
Template SS confidence
		409	410	.	.	.	.	.	.	.	.	.	420	.	.	.	.	.	.	.	.	.	430	.	.	.	.					.	.	.	.	.	440	.	.	.	.	.

No model constructed - rank, confidence too low

---

Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
© Structural Bioinformatics Group, Imperial College, London Lawrence Kelley, Benjamin Jefferys   Disclaimer Terms and Conditions