Job Description P32171 Confidence 100.00% Date Thu Jan 5 11:49:51 GMT 2012 Rank 14 Aligned Residues 463 % Identity 17% Template c3ezwD_ PDB info PDB header:transferase Chain: D: PDB Molecule:glycerol kinase; PDBTitle: crystal structure of a hyperactive escherichia coli glycerol kinase2 mutant gly230 --> asp obtained using microfluidic crystallization3 devices Resolution 2.00 Å

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		2	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60	.	.	.	.	.	.	.	.	.					70	.	.	.	.	.	.	.
Predicted Secondary structure																																																																						.	.	.	.
Query SS confidence																																																																						.	.	.	.
Query Sequence		T	F	R	N	C	V	A	V	D	L	G	A	S	S	G	R	V	M	L	A	R	Y	E	R	E	C	R	S	L	T	L	R	E	I	H	R	F	N	N	G	L	H	S	Q	N	G	Y	V	T	W	D	V	D	S	L	E	S	A	I	R	L	G	L	N	K	V	C	E	.	.	.	.	E	G	I	R	I	D	S	I
Query Conservation																																																																						.	.	.	.
Alig confidence																																					.	.	.	.																														.	.	.	.
Template Conservation																																					.	.	.	.
Template Sequence		E	K	K	Y	I	V	A	L	D	Q	G	T	T	S	S	R	A	V	V	M	D	H	D	A	N	I	I	S	V	S	Q	R	E	F	E	.	.	.	.	Q	I	Y	P	K	P	G	W	V	E	H	D	P	M	E	I	W	A	T	Q	S	S	T	L	V	E	V	L	A	K	A	D	I	S	S	D	Q	I	A	A	I
Template Known Secondary structure													S	S									T	T													.	.	.	.		B			S	T	T																								T	T			G	G	G
Template Predicted Secondary structure																																					.	.	.	.
Template SS confidence
		2	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.					.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60	.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.
		78	.	80	.	.	.	.	.	.	.	.	.	90	.		.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120	.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.
Predicted Secondary structure																.
Query SS confidence																.
Query Sequence		G	I	D	T	W	G	V	D	F	V	L	L	D	Q	.	Q	G	Q	R	V	G	L	P	V	A	Y	R	D	S	R	T	N	G	L	M	A	Q	A	Q	Q	Q	L	G	K	R	D	I	Y	Q	R	S	G	I	Q	F	L	P	F	N	T	L	Y	Q	L	R	A	L	T	E	Q	Q	P	E	L	I	P	H	I	A	H
Query Conservation																.
Alig confidence																.
Template Conservation
Template Sequence		G	I	T	N	Q	R	E	T	T	I	V	W	E	K	E	T	G	K	P	I	Y	N	A	I	V	W	Q	C	R	R	T	A	E	I	C	E	H	L	K	R	D	G	L	E	D	Y	I	R	S	N	T	G	L	V	I	D	P	Y	F	S	G	T	K	V	K	W	I	L	D	H	V	E	G	S	R	E	R	A	R	R
Template Known Secondary structure							S	S		B					T	T	T			B	S	S					T	T			T	T										T	T										S			S	S	T	T	S												S	T	T							T
Template Predicted Secondary structure
Template SS confidence
		78	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120	.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.
						157	.	.	160	.	.	.	.	.	.	.	.	.	170			.	.	.	.	.	.	.	.	.	180	.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230
Predicted Secondary structure		.	.	.	.															.	.
Query SS confidence		.	.	.	.															.	.
Query Sequence		.	.	.	.	A	L	L	M	P	D	Y	F	S	Y	R	L	T	G	.	.	K	M	N	W	E	Y	T	N	A	T	T	T	Q	L	V	N	I	N	S	D	D	W	D	E	S	L	L	A	W	S	G	A	N	K	A	W	F	G	R	P	T	H	P	G	N	V	I	G	H	W	I	C	P	Q	G	N	E	I	P	V
Query Conservation		.	.	.	.															.	.
Alig confidence		.	.	.	.															.	.																																																				.	.	.	.	.
Template Conservation																																																																									.	.	.	.	.
Template Sequence		G	E	L	L	F	G	T	V	D	T	W	L	I	W	K	M	T	Q	G	R	V	H	V	T	D	Y	T	N	A	S	R	T	M	L	F	N	I	H	T	L	D	W	D	D	K	M	L	E	V	L	D	I	P	R	E	M	L	P	E	V	R	R	S	S	E	V	Y	G	Q	T	N	.	.	.	.	.	R	I	P	I
Template Known Secondary structure		T	S															T	T	T	S										G	G	G	S	S			T	T	T	T										T	T			G	G	G							S									.	.	.	.	.
Template Predicted Secondary structure																																																																									.	.	.	.	.
Template SS confidence
		158	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180	.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.						.	230	.	.
		231	.	.	.	.	.	.	.	.	240	.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.		.	.	.	.	290	.	.	.	.	.	.	.	.	.	300	.	.	.	.	.	.	.	.	.
Predicted Secondary structure																																																									.
Query SS confidence																																																									.
Query Sequence		V	A	V	A	S	H	D	T	A	S	A	V	I	A	S	P	L	N	G	S	R	A	A	Y	L	S	S	G	T	W	S	L	M	G	F	E	S	Q	T	P	F	T	N	D	T	A	L	A	A	N	I	T	N	E	G	.	G	A	E	G	R	Y	R	V	L	K	N	I	M	G	L	W	L	L	Q	R	V	L	Q	E
Query Conservation																																																									.
Alig confidence				.																																																					.
Template Conservation				.
Template Sequence		S	G	.	I	A	G	D	Q	Q	A	A	L	F	G	Q	L	C	V	K	E	G	M	A	K	N	T	Y	G	T	G	C	F	M	L	M	N	T	G	E	K	A	V	K	S	E	N	G	L	L	T	T	I	A	C	G	P	T	G	E	V	N	Y	A	L	E	G	A	V	F	M	A	G	A	S	I	Q	W	L	R	D
Template Known Secondary structure				.												T	T			S	T	T							S	S	S								T	T	S					S	S	S									T	T	S											S		S
Template Predicted Secondary structure				.
Template SS confidence
		240	.		.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300	.	.	.	.	.	.	.	.	.	310	.	.	.	.	.	.	.	.
		310	.	.	.	.	.	.	.	.	.	320	.	.	.	.	.	.	.	.	.	330	.	.	.	.	.	.	.	.	.	340	.	.	.	.	.	.	.	.	.	350	.	.	.	.	.	.	.	.	.	360	.	.	.	.	.	.	.	.	.	370	.	.	.	.	.	.	.	.	.	380	.	.	.	.	.	.	.	.	.
Predicted Secondary structure
Query SS confidence
Query Sequence		Q	Q	I	N	D	L	P	A	L	I	S	A	T	Q	A	L	P	A	C	R	F	I	I	N	P	N	D	D	R	F	I	N	P	E	T	M	C	S	E	I	Q	A	A	C	R	E	T	A	Q	P	I	P	E	S	D	A	E	L	A	R	C	I	F	D	S	L	A	L	L	Y	A	D	V	L	H	E	L	A	Q	L
Query Conservation
Alig confidence																	.											.	.	.
Template Conservation																	.											.	.	.
Template Sequence		E	M	K	L	I	D	S	E	Y	F	A	T	K	V	Q	.	N	T	N	G	V	Y	V	V	P	A	.	.	.	F	T	G	L	G	A	P	Y	W	D	P	Y	A	R	G	A	I	F	G	L	T	R	G	V	N	A	N	H	I	I	R	A	T	L	E	S	I	A	Y	Q	T	R	D	V	L	E	A	M	Q	A	D
Template Known Secondary structure		T	S											T	S	S	.	S		T	T							.	.	.	T	T		B		T	T	T		B	T	T										T	T
Template Predicted Secondary structure																	.											.	.	.
Template SS confidence
		319	320	.	.	.	.	.	.	.	.	.	330	.	.	.		.	.	.	.	.	.	340	.	.	.				.	.	.	.	.	.	350	.	.	.	.	.	.	.	.	.	360	.	.	.	.	.	.	.	.	.	370	.	.	.	.	.	.	.	.	.	380	.	.	.	.	.	.	.	.	.	390	.	.	.	.
		390	.	.	.	.	.	.	.	.	.	400	.	.	.	.	.	.	.	.	.	410	.	.	.	.	.	.	.	.	.	420	.	.	.	.	.	.	.	.	.	430	.	.	.	.	.	.	.	.	.	440	.	.	.	.	.	.	.	.	.	450	.	.	.	.	.	.	.	.	.	460	.	.	.	.	.	.	.	.	.
Predicted Secondary structure
Query SS confidence
Query Sequence		R	G	E	D	F	S	Q	L	H	I	V	G	G	G	C	Q	N	T	L	L	N	Q	L	C	A	D	A	C	G	I	R	V	I	A	G	P	V	E	A	S	T	L	G	N	I	G	I	Q	L	M	T	L	D	E	L	N	N	V	D	D	F	R	Q	V	V	S	T	T	A	N	L	T	T	F	T	P	N	P	D	S
Query Conservation
Alig confidence																																				.	.																												.	.	.	.	.	.
Template Conservation																																				.	.																												.	.	.	.	.	.
Template Sequence		S	G	I	R	L	H	A	L	R	V	D	G	G	A	V	A	N	N	F	L	M	Q	F	Q	S	D	I	L	G	T	R	V	E	R	.	.	P	E	V	T	A	L	G	A	A	Y	L	A	G	L	A	V	G	F	W	Q	N	L	D	E	L	Q	E	.	.	.	.	.	.	I	E	R	E	F	R	P	G	I	E	T
Template Known Secondary structure							S						S	G	G	G	G													T	S					.	.																	T	S	S	S						T		.	.	.	.	.	.									S
Template Predicted Secondary structure																																				.	.																												.	.	.	.	.	.
Template SS confidence
		399	400	.	.	.	.	.	.	.	.	.	410	.	.	.	.	.	.	.	.	.	420	.	.	.	.	.	.	.	.	.	430	.	.			.	.	.	.	.	.	.	440	.	.	.	.	.	.	.	.	.	450	.	.	.	.	.	.	.	.	.							460	.	.	.	.	.	.	.	.	.	470
		470	.	.	.	.	.	.	.	.	.	480	.	.	.	.	.	.	.	.
Predicted Secondary structure
Query SS confidence
Query Sequence		E	I	A	H	Y	V	A	Q	I	H	S	T	R	Q	T	K	E	L	C
Query Conservation
Alig confidence				.	.
Template Conservation				.	.
Template Sequence		T	E	.	.	R	N	Y	R	Y	A	G	W	K	K	A	V	K	R	A
Template Known Secondary structure				.	.
Template Predicted Secondary structure				.	.
Template SS confidence
		477	.			.	480	.	.	.	.	.	.	.	.	.	490	.	.	.