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Job DescriptionP00582
Confidence100.00%DateThu Jan 5 10:56:46 GMT 2012
Rank1Aligned Residues814
% Identity41%Templatec1cmwA_
PDB info PDB header:transferaseChain: A: PDB Molecule:protein (dna polymerase i); PDBTitle: crystal structure of taq dna-polymerase shows a new2 orientation for the structure-specific nuclease domain
Resolution2.60 Å

  Insertion relative to template
  Deletion relative to template
  Catalytic residue from the CSA
 
Detailed help on interpreting your alignment


   5....10.........20.........30.........40.........50.........60.........70.........80....
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Query Sequence  PQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLNMLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPM
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Template Sequence  PKGRVLLVDGHHLAYRTFHALKGLTTSRGEPVQAVYGFAKSLLKALKE. DGDAVIVVFDAKAPSFRHEAYGGYKAGRAPT
Template Known Secondary structure 

TTTSSTT
B
TTS
B
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S


SSS
TTGGGGSTTTS




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   10.........20.........30.........40.........50....... ..60.........70.........80........
 
   85....90.........100.........110.........120.........130.........140.........150.........160....
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Query Sequence  PDDLRAQIEPLHAMVKAMGLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTILGPEE
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Template Conservation 

 
  
   
 
 
   

      
 









      
  
 
 
 



 


   
 
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Template Sequence  PEDFPRQLALIKELVDLLGLARLEVPGYEADDVLASLAKKAEKEGYEVRILTADKDLYQLLSDRIHVLHP. EGYLITPAW
Template Known Secondary structure 
SSTT


TTS
TT
SSGGGGGS

SS

S.SSS
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   8990.........100.........110.........120.........130.........140.........150........ .160.......
 
   165....170.........180.........190.........200.........210.........220.........230.........240....
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Query Sequence  VVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEPEKIAGLSFRGAKTMAAKLEQNKEVAY
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Template Sequence  LWEKYGLRPDQWADYRALTGDESDNLPGVKGIGEKTARKLLEEWGSLEALLKNLDRLKP. . . . . . . AIREKILAHMDDLK
Template Known Secondary structure  SS


GGGTT

SSS






STT
TTTTTGGGGGTT
SSSSS




T.......TTTTSTTTTT
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   168.170.........180.........190.........200.........210.........220...... ...230.........240
 
   245....250.........260.........270.........280.........290.........300.........310.........320....
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Query Sequence  LSYQLATIKTDVELELTCEQLEVQQPAAEELLGLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVADEAPEVTATV
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Template Sequence  LSWDLAKVRTDLPLEVDFAKRR. . EPDRERLRAFLERLEFGSLLHEFGLLESPALEEAPWPPPE. . . . . . . . . . . . . . . .
Template Known Secondary structure  TGGGGG


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TT
GGGTT
S








SSS
T................
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   241........250.........260.. .......270.........280.........290.........300..
 
   325....330.........340.........350.........360.........370.........380.........390.........400....
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Query Sequence  ISYDNYVTILDEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDAPDQISRERALEL
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Template Conservation  ..........................                          .       ....................
Template Sequence  . . . . . . . . . . . . . . . . . . . . . . . . . . GAFVGFVLSRKEPMWADLLALAAARG. GRVHRAP. . . . . . . . . . . . . . . . . . . .
Template Known Secondary structure  ..........................T


SSS
TTT


T.T

S....................
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   304.....310.........320......... 330......
 
   405....410.........420........ .430.........440.........450.........460.........470.........480...
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Query Sequence  LKPLLEDEKALKVGQNLKYDRGIL. ANYGIELRGIAFDTMLESYILNSVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQ
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Template Sequence  . EPYKALRDLKEARGLLAKDLSVLALREGLGLPPG. DDPMLLAYLLDPS. . NTTPEGVARRYGGEWT. . . . . . . . . . . . .
Template Known Secondary structure  .

SSB
STTTT



B
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TT..

STS



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   337..340.........350.........360.........370 .........380... ......390.........
 
   484.....490.........500.........510.........520.........530.........540.........550.........560...
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Query Sequence  LTFNQIALEEAGRYAAEDADVTLQLHLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRL
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Template Sequence  . . . . . . . . . . . . EEAGERAALSERLFANLWGRLEGEERLLWLYREVERPLSAVLAHMEATGVRLDVAYLRALSLEV. . . .
Template Known Secondary structure  ............S
TT
T
B
B
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   400.........410.........420.........430.........440.........450.........460...
 
   564.....570.........580.........590.........600.. .......610.........620.........630.........640..
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Query Sequence  AELEKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKT. PGGAPSTSEEVLEELALDYPLPKVILEYRGLAKLKSTYTD
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Template Sequence  . ALEAEVFRLAGHPFNLNSRDQLERVLFDELGLPAIGKTEKTGKRSTSAAVLEALREAHPIVEKILQYRELTKLKSTYID
Template Known Secondary structure  .

TTSS


TT
S






SSS
SSTTTTTTTTTTT
STS
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   464.....470.........480.........490.........500.........510.........520.........530.........540..
 
   643......650.........660.........670.........680.........690.........700.........710.........720..
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Query Sequence  KLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVRNEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKG
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Template Sequence  PLPDLIHPRTGRLHTRFNQTATATGRLSSSDPNLQNIPVRTPLGQRIRRAFIAEEGWLLVALDYSQIELRVLAHLSGDEN
Template Known Secondary structure  STTTSB
TTT
SB

SSS
SSS

SS
GGGS
TTSTBB

TTTTT
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   548.550.........560.........570.........580.........590.........600.........610.........620.......
 
   723......730.........740.........750.........760.........770.........780.........790.........800..
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Query Sequence  LLTAFAEGKDIHRATAAEVFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGVLEYM
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Template Sequence  LIRVFQEGRDIHTETASWMFGVPREAVDPLMRRAAKTINFGVLYGMSAHRLSQELAIPYEEAQAFIERYFQSFPKVRAWI
Template Known Secondary structure  T

T

GGG

TTS


SS


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   628.630.........640.........650.........660.........670.........680.........690.........700.......
 
   803......810.........820.........830.........840.........850.........860.........870.........880..
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Query Sequence  ERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADIIKRAMIAVDAWLQAEQPRVRMIMQVHD
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Template Sequence  EKTLEEGRRRGYVETLFGRRRYVPDLEARVKSVREAAERMAFNMPVQGTAADLMKLAMVKLFPRLEE. . MGARMLLQVHD
Template Known Secondary structure  TSB


SS



B
GGGG
SSTT..TT
TT
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   708.710.........720.........730.........740.........750.........760.........770.... .....780.....
 
   883......890.........900.........910.........920........
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Query Sequence  ELVFEVHKDDVDAVAKQIHQLMENCTRLDVPLLVEVGSGENWDQAH
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Template Sequence  ELVLEAPKERAEAVARLAKEVMEGVYPLAVPLEVEVGIGEDWLSAK
Template Known Secondary structure  TTTTSS

SS




SB
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   786...790.........800.........810.........820.........830.
 
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Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
 
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Lawrence Kelley, Benjamin Jefferys 
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