Secondary structure and disorder prediction

		1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	Q	P	G	K	R	F	L	V	W	L	A	G	L	S	V	L	G	F	L	A	T	D	M	Y	L	P	A	F	A	A	I	Q	A	D	L	Q	T	P	A	S	A	V	S	A	S	L	S	L	F	L	A	G	F	A	A	A	Q	L	L
Secondary structure
SS confidence
Disorder		?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		W	G	P	L	S	D	R	Y	G	R	K	P	V	L	L	I	G	L	T	I	F	A	L	G	S	L	G	M	L	W	V	E	N	A	A	T	L	L	V	L	R	F	V	Q	A	V	G	V	C	A	A	A	V	I	W	Q	A	L	V	T
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		D	Y	Y	P	S	Q	K	V	N	R	I	F	A	A	I	M	P	L	V	G	L	S	P	A	L	A	P	L	L	G	S	W	L	L	V	H	F	S	W	Q	A	I	F	A	T	L	F	A	I	T	V	V	L	I	L	P	I	F	W	L
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		K	P	T	T	K	A	R	N	N	S	Q	D	G	L	T	F	T	D	L	L	R	S	K	T	Y	R	G	N	V	L	I	Y	A	A	C	S	A	S	F	F	A	W	L	T	G	S	P	F	I	L	S	E	M	G	Y	S	P	A	V	I
Secondary structure
SS confidence
Disorder						?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300
Sequence		G	L	S	Y	V	P	Q	T	I	A	F	L	I	G	G	Y	G	C	R	A	A	L	Q	K	W	Q	G	K	Q	L	L	P	W	L	L	V	L	F	A	V	S	V	I	A	T	W	A	A	G	F	I	S	H	V	S	L	V	E	I	L
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	310	.	.	.	.	.	.	.	.	.	320	.	.	.	.	.	.	.	.	.	330	.	.	.	.	.	.	.	.	.	340	.	.	.	.	.	.	.	.	.	350	.	.	.	.	.	.	.	.	.	360
Sequence		I	P	F	C	V	M	A	I	A	N	G	A	I	Y	P	I	V	V	A	Q	A	L	R	P	F	P	H	A	T	G	R	A	A	A	L	Q	N	T	L	Q	L	G	L	C	F	L	A	S	L	V	V	S	W	L	I	S	I	S	T	P
Secondary structure
SS confidence
Disorder																									?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	370	.	.	.	.	.	.	.	.	.	380	.	.	.	.	.	.	.	.	.	390	.	.	.	.	.	.	.	.	.	400	.	.	.
Sequence		L	L	T	T	T	S	V	M	L	S	T	V	V	L	V	A	L	G	Y	M	M	Q	R	C	E	E	V	G	C	Q	N	H	G	N	A	E	V	A	H	S	E	S	H
Secondary structure
SS confidence
Disorder																									?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?
Disorder confidence

Confidence Key
High(9)											Low (0)

?	Disordered
	Alpha helix
	Beta strand

Domain analysis

Hover over an aligned region to see model and summary info

Please note, only up to the top 20 hits are modelled to reduce computer load

Rank Aligned region

PDB 2gfp chain A Region: 8 - 374 Aligned: 365 Modelled: 367 Confidence: 100.0% Identity: 21% PDB header:membrane protein Chain: A: PDB Molecule:multidrug resistance protein d; PDBTitle: structure of the multidrug transporter emrd from2 escherichia coli Phyre2 PDB 1pw4 chain A Region: 5 - 395 Aligned: 390 Modelled: 391 Confidence: 100.0% Identity: 13% Fold: MFS general substrate transporter Superfamily: MFS general substrate transporter Family: Glycerol-3-phosphate transporter Phyre2 PDB 1pv7 chain A Region: 4 - 397 Aligned: 394 Modelled: 394 Confidence: 100.0% Identity: 10% Fold: MFS general substrate transporter Superfamily: MFS general substrate transporter Family: LacY-like proton/sugar symporter Phyre2 PDB 3o7p chain A Region: 5 - 384 Aligned: 378 Modelled: 380 Confidence: 100.0% Identity: 12% PDB header:transport protein Chain: A: PDB Molecule:l-fucose-proton symporter; PDBTitle: crystal structure of the e.coli fucose:proton symporter, fucp (n162a) Phyre2 PDB 2xut chain C Region: 6 - 381 Aligned: 374 Modelled: 376 Confidence: 100.0% Identity: 13% PDB header:transport protein Chain: C: PDB Molecule:proton/peptide symporter family protein; PDBTitle: crystal structure of a proton dependent oligopeptide (pot)2 family transporter. Phyre2 PDB 2g9p chain A Region: 60 - 73 Aligned: 14 Modelled: 14 Confidence: 22.1% Identity: 43% PDB header:antimicrobial protein Chain: A: PDB Molecule:antimicrobial peptide latarcin 2a; PDBTitle: nmr structure of a novel antimicrobial peptide, latarcin 2a,2 from spider (lachesana tarabaevi) venom Phyre2 PDB 2knc chain A Region: 361 - 401 Aligned: 41 Modelled: 41 Confidence: 16.7% Identity: 12% PDB header:cell adhesion Chain: A: PDB Molecule:integrin alpha-iib; PDBTitle: platelet integrin alfaiib-beta3 transmembrane-cytoplasmic2 heterocomplex Phyre2 PDB 3qnq chain D Region: 348 - 400 Aligned: 53 Modelled: 53 Confidence: 16.5% Identity: 6% PDB header:membrane protein, transport protein Chain: D: PDB Molecule:pts system, cellobiose-specific iic component; PDBTitle: crystal structure of the transporter chbc, the iic component from the2 n,n'-diacetylchitobiose-specific phosphotransferase system Phyre2 PDB 2xq2 chain A Region: 263 - 403 Aligned: 136 Modelled: 136 Confidence: 7.5% Identity: 7% PDB header:transport protein Chain: A: PDB Molecule:sodium/glucose cotransporter; PDBTitle: structure of the k294a mutant of vsglt Phyre2 PDB 3pro chain C domain 1 Region: 28 - 69 Aligned: 42 Modelled: 42 Confidence: 7.3% Identity: 14% Fold: Alpha-lytic protease prodomain-like Superfamily: Alpha-lytic protease prodomain Family: Alpha-lytic protease prodomain Phyre2 PDB 1lzi chain A Region: 399 - 403 Aligned: 5 Modelled: 5 Confidence: 5.9% Identity: 80% Fold: Nucleotide-diphospho-sugar transferases Superfamily: Nucleotide-diphospho-sugar transferases Family: alpha-1,3-galactosyltransferase-like Phyre2 1 c2gfpA_ 2 d1pw4a_ 3 d1pv7a_ 4 c3o7pA_ 5 c2xutC_ 6 c2g9pA_ 7 c2kncA_ 8 c3qnqD_ 9 c2xq2A_ 10 d3proc1 11 d1lzia_

Detailed template information

# Template Alignment Coverage 3D Model Confidence % i.d. Template Information

1 c2gfpA_ 100.0 21 PDB header:membrane protein Chain: A: PDB Molecule:multidrug resistance protein d; PDBTitle: structure of the multidrug transporter emrd from2 escherichia coli 2 d1pw4a_ 100.0 13 Fold:MFS general substrate transporter Superfamily:MFS general substrate transporter Family:Glycerol-3-phosphate transporter 3 d1pv7a_ 100.0 10 Fold:MFS general substrate transporter Superfamily:MFS general substrate transporter Family:LacY-like proton/sugar symporter 4 c3o7pA_ 100.0 12 PDB header:transport protein Chain: A: PDB Molecule:l-fucose-proton symporter; PDBTitle: crystal structure of the e.coli fucose:proton symporter, fucp (n162a) 5 c2xutC_ 100.0 13 PDB header:transport protein Chain: C: PDB Molecule:proton/peptide symporter family protein; PDBTitle: crystal structure of a proton dependent oligopeptide (pot)2 family transporter. 6 c2g9pA_ 22.1 43 PDB header:antimicrobial protein Chain: A: PDB Molecule:antimicrobial peptide latarcin 2a; PDBTitle: nmr structure of a novel antimicrobial peptide, latarcin 2a,2 from spider (lachesana tarabaevi) venom 7 c2kncA_ 16.7 12 PDB header:cell adhesion Chain: A: PDB Molecule:integrin alpha-iib; PDBTitle: platelet integrin alfaiib-beta3 transmembrane-cytoplasmic2 heterocomplex 8 c3qnqD_ 16.5 6 PDB header:membrane protein, transport protein Chain: D: PDB Molecule:pts system, cellobiose-specific iic component; PDBTitle: crystal structure of the transporter chbc, the iic component from the2 n,n'-diacetylchitobiose-specific phosphotransferase system 9 c2xq2A_ 7.5 7 PDB header:transport protein Chain: A: PDB Molecule:sodium/glucose cotransporter; PDBTitle: structure of the k294a mutant of vsglt 10 d3proc1 7.3 14 Fold:Alpha-lytic protease prodomain-like Superfamily:Alpha-lytic protease prodomain Family:Alpha-lytic protease prodomain 11 d1lzia_ 5.9 80 Fold:Nucleotide-diphospho-sugar transferases Superfamily:Nucleotide-diphospho-sugar transferases Family:alpha-1,3-galactosyltransferase-like

Binding site prediction

Due to computational demand, binding site predictions are not run for batch jobs

If you want to predict binding sites, please manually submit your model of choice to 3DLigandSite

Transmembrane helix prediction

Transmembrane helices have been predicted in your sequence to adopt the topology shown below

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Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
If you use the binding site predictions from 3DLigandSite, please also cite:
3DLigandSite: predicting ligand-binding sites using similar structures.
Wass MN, Kelley LA and Sternberg MJ Nucleic Acids Research 38, W469-73 (2010) [PubMed]
© Structural Bioinformatics Group Imperial College London Lawrence Kelley, Benjamin Jefferys Disclaimer Terms and Conditions	Component software Template detection: HHpred 1.51 Secondary structure prediction: Psi-pred 2.5 Disorder prediction: Disopred 2.4 Transmembrane prediction: Memsat_SVM Multi-template modelling and ab initio: Poing 1.0