Secondary structure and disorder prediction

		1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	G	I	N	E	I	I	M	Y	I	M	M	F	F	M	L	I	A	A	V	D	R	I	L	S	Q	F	G	G	S	A	R	F	L	G	K	F	G	K	S	I	E	G	S	G	G	Q	F	E	E	G	F	M	A	M	G	A	L	G	L
Secondary structure
SS confidence
Disorder		?	?																										?	?	?	?								?		?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		A	M	V	G	M	T	A	L	A	P	V	L	A	H	V	L	G	P	V	I	I	P	V	Y	E	M	L	G	A	N	P	S	M	F	A	G	T	L	L	A	C	D	M	G	G	F	F	L	A	K	E	L	A	G	G	D	V	A	A	W
Secondary structure
SS confidence
Disorder																												?		?	?														?									?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		L	Y	S	G	L	I	L	G	S	M	M	G	P	T	I	V	F	S	I	P	V	A	L	G	I	I	E	P	S	D	R	R	Y	L	A	L	G	V	L	A	G	I	V	T	I	P	I	G	C	I	A	G	G	L	V	A	M	Y	S	G
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		V	Q	I	N	G	Q	P	V	E	F	T	F	A	L	I	L	M	N	M	I	P	V	I	I	V	A	I	L	V	A	L	G	L	K	F	I	P	E	K	M	I	N	G	F	Q	I	F	A	K	F	L	V	A	L	I	T	L	G	L	A
Secondary structure
SS confidence
Disorder		?		?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300
Sequence		A	A	V	V	K	F	L	L	G	W	E	L	I	P	G	L	D	P	I	F	M	A	P	G	D	K	P	G	E	V	M	R	A	I	E	V	I	G	S	I	S	C	V	L	L	G	A	Y	P	M	V	L	L	L	T	R	W	F	E	K
Secondary structure
SS confidence
Disorder																									?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	310	.	.	.	.	.	.	.	.	.	320	.	.	.	.	.	.	.	.	.	330	.	.	.	.	.	.	.	.	.	340	.	.	.	.	.	.	.	.	.	350	.	.	.	.	.	.	.	.	.	360
Sequence		P	L	M	S	V	G	K	V	L	N	M	N	N	I	A	A	A	G	M	V	A	T	L	A	N	N	I	P	M	F	G	M	M	K	Q	M	D	T	R	G	K	V	I	N	C	A	F	A	V	S	A	A	F	A	L	G	D	H	L	G
Secondary structure
SS confidence
Disorder												?
Disorder confidence
		.	.	.	.	.	.	.	.	.	370	.	.	.	.	.	.	.	.	.	380	.	.	.	.	.	.	.	.	.	390	.	.	.	.	.	.	.	.	.	400	.	.	.	.	.	.	.	.
Sequence		F	A	A	A	N	M	N	A	M	I	F	P	M	I	V	G	K	L	I	G	G	V	T	A	I	G	V	A	M	M	L	V	P	K	E	D	A	T	A	T	K	T	E	A	E	A	Q	S
Secondary structure
SS confidence
Disorder																																										?	?	?		?	?	?	?
Disorder confidence

Confidence Key
High(9)											Low (0)

?	Disordered
	Alpha helix
	Beta strand

Domain analysis

Hover over an aligned region to see model and summary info

Please note, only up to the top 20 hits are modelled to reduce computer load

Rank Aligned region

PDB 2hly chain A domain 1 Region: 344 - 358 Aligned: 15 Modelled: 15 Confidence: 35.4% Identity: 33% Fold: Cysteine proteinases Superfamily: Cysteine proteinases Family: Atu2299-like Phyre2 PDB 3qnq chain D Region: 344 - 408 Aligned: 65 Modelled: 65 Confidence: 35.0% Identity: 14% PDB header:membrane protein, transport protein Chain: D: PDB Molecule:pts system, cellobiose-specific iic component; PDBTitle: crystal structure of the transporter chbc, the iic component from the2 n,n'-diacetylchitobiose-specific phosphotransferase system Phyre2 PDB 3pjz chain A Region: 255 - 407 Aligned: 150 Modelled: 153 Confidence: 34.3% Identity: 14% PDB header:transport protein Chain: A: PDB Molecule:potassium uptake protein trkh; PDBTitle: crystal structure of the potassium transporter trkh from vibrio2 parahaemolyticus Phyre2 PDB 1j4n chain A Region: 337 - 405 Aligned: 60 Modelled: 69 Confidence: 21.8% Identity: 22% Fold: Aquaporin-like Superfamily: Aquaporin-like Family: Aquaporin-like Phyre2 PDB 3ajv chain A Region: 312 - 343 Aligned: 32 Modelled: 32 Confidence: 17.3% Identity: 16% PDB header:hydrolase Chain: A: PDB Molecule:putative uncharacterized protein; PDBTitle: splicing endonuclease from aeropyrum pernix Phyre2 PDB 2ntx chain B Region: 334 - 354 Aligned: 21 Modelled: 21 Confidence: 16.3% Identity: 29% PDB header:signaling protein Chain: B: PDB Molecule:emb cab41934.1; Phyre2 PDB 3ai9 chain X Region: 352 - 363 Aligned: 12 Modelled: 12 Confidence: 16.0% Identity: 42% PDB header:transferase Chain: X: PDB Molecule:upf0217 protein mj1640; PDBTitle: crystal structure of duf358 protein reveals a putative spout-class2 rrna methyltransferase Phyre2 PDB 2ka2 chain A Region: 196 - 213 Aligned: 18 Modelled: 18 Confidence: 15.2% Identity: 33% PDB header:membrane protein Chain: A: PDB Molecule:bcl2/adenovirus e1b 19 kda protein-interacting PDBTitle: solution nmr structure of bnip3 transmembrane peptide dimer2 in detergent micelles with his173-ser172 intermonomer3 hydrogen bond restraints Phyre2 PDB 2ka2 chain B Region: 196 - 213 Aligned: 18 Modelled: 18 Confidence: 15.2% Identity: 33% PDB header:membrane protein Chain: B: PDB Molecule:bcl2/adenovirus e1b 19 kda protein-interacting PDBTitle: solution nmr structure of bnip3 transmembrane peptide dimer2 in detergent micelles with his173-ser172 intermonomer3 hydrogen bond restraints Phyre2 PDB 2ka1 chain B Region: 196 - 213 Aligned: 18 Modelled: 18 Confidence: 15.2% Identity: 33% PDB header:membrane protein Chain: B: PDB Molecule:bcl2/adenovirus e1b 19 kda protein-interacting PDBTitle: solution nmr structure of bnip3 transmembrane peptide dimer2 in detergent micelles Phyre2 PDB 2ka1 chain A Region: 196 - 213 Aligned: 18 Modelled: 18 Confidence: 15.2% Identity: 33% PDB header:membrane protein Chain: A: PDB Molecule:bcl2/adenovirus e1b 19 kda protein-interacting PDBTitle: solution nmr structure of bnip3 transmembrane peptide dimer2 in detergent micelles Phyre2 PDB 2qwv chain A domain 1 Region: 352 - 363 Aligned: 12 Modelled: 12 Confidence: 15.0% Identity: 25% Fold: alpha/beta knot Superfamily: alpha/beta knot Family: AF1056-like Phyre2 PDB 2qmm chain A domain 1 Region: 342 - 363 Aligned: 22 Modelled: 22 Confidence: 13.6% Identity: 27% Fold: alpha/beta knot Superfamily: alpha/beta knot Family: AF1056-like Phyre2 PDB 1acc chain A Region: 25 - 43 Aligned: 19 Modelled: 19 Confidence: 13.1% Identity: 16% Fold: Anthrax protective antigen Superfamily: Anthrax protective antigen Family: Anthrax protective antigen Phyre2 PDB 1ig8 chain A domain 1 Region: 96 - 105 Aligned: 10 Modelled: 10 Confidence: 11.7% Identity: 60% Fold: Ribonuclease H-like motif Superfamily: Actin-like ATPase domain Family: Hexokinase Phyre2 PDB 2j5d chain A Region: 195 - 213 Aligned: 19 Modelled: 19 Confidence: 11.2% Identity: 32% PDB header:membrane protein Chain: A: PDB Molecule:bcl2/adenovirus e1b 19 kda protein-interacting PDBTitle: nmr structure of bnip3 transmembrane domain in lipid2 bicelles Phyre2 PDB 1fx8 chain A Region: 348 - 408 Aligned: 55 Modelled: 61 Confidence: 11.1% Identity: 20% Fold: Aquaporin-like Superfamily: Aquaporin-like Family: Aquaporin-like Phyre2 PDB 1lda chain A Region: 348 - 408 Aligned: 55 Modelled: 61 Confidence: 11.1% Identity: 20% PDB header:transport protein Chain: A: PDB Molecule:glycerol uptake facilitator protein; PDBTitle: crystal structure of the e. coli glycerol facilitator (glpf) without2 substrate glycerol Phyre2 PDB 3zy6 chain A Region: 101 - 136 Aligned: 28 Modelled: 36 Confidence: 11.1% Identity: 29% PDB header:transferase Chain: A: PDB Molecule:putative gdp-fucose protein o-fucosyltransferase 1; PDBTitle: crystal structure of pofut1 in complex with gdp-fucose2 (crystal-form-ii) Phyre2 PDB 1cza chain N domain 1 Region: 96 - 105 Aligned: 10 Modelled: 10 Confidence: 10.8% Identity: 50% Fold: Ribonuclease H-like motif Superfamily: Actin-like ATPase domain Family: Hexokinase Phyre2 1 d2hlya1 2 c3qnqD_ 3 c3pjzA_ 4 d1j4na_ 5 c3ajvA_ 6 c2ntxB_ 7 c3ai9X_ 8 c2ka2A_ 9 c2ka2B_ 10 c2ka1B_ 11 c2ka1A_ 12 d2qwva1 13 d2qmma1 14 d1acca_ 15 d1ig8a1 16 c2j5dA_ 17 d1fx8a_ 18 c1ldaA_ 19 c3zy6A_ 20 d1czan1 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54

Detailed template information

# Template Alignment Coverage 3D Model Confidence % i.d. Template Information

1 d2hlya1 35.4 33 Fold:Cysteine proteinases Superfamily:Cysteine proteinases Family:Atu2299-like 2 c3qnqD_ 35.0 14 PDB header:membrane protein, transport protein Chain: D: PDB Molecule:pts system, cellobiose-specific iic component; PDBTitle: crystal structure of the transporter chbc, the iic component from the2 n,n'-diacetylchitobiose-specific phosphotransferase system 3 c3pjzA_ 34.3 14 PDB header:transport protein Chain: A: PDB Molecule:potassium uptake protein trkh; PDBTitle: crystal structure of the potassium transporter trkh from vibrio2 parahaemolyticus 4 d1j4na_ 21.8 22 Fold:Aquaporin-like Superfamily:Aquaporin-like Family:Aquaporin-like 5 c3ajvA_ 17.3 16 PDB header:hydrolase Chain: A: PDB Molecule:putative uncharacterized protein; PDBTitle: splicing endonuclease from aeropyrum pernix 6 c2ntxB_ 16.3 29 PDB header:signaling protein Chain: B: PDB Molecule:emb cab41934.1; 7 c3ai9X_ 16.0 42 PDB header:transferase Chain: X: PDB Molecule:upf0217 protein mj1640; PDBTitle: crystal structure of duf358 protein reveals a putative spout-class2 rrna methyltransferase 8 c2ka2A_ 15.2 33 PDB header:membrane protein Chain: A: PDB Molecule:bcl2/adenovirus e1b 19 kda protein-interacting PDBTitle: solution nmr structure of bnip3 transmembrane peptide dimer2 in detergent micelles with his173-ser172 intermonomer3 hydrogen bond restraints 9 c2ka2B_ 15.2 33 PDB header:membrane protein Chain: B: PDB Molecule:bcl2/adenovirus e1b 19 kda protein-interacting PDBTitle: solution nmr structure of bnip3 transmembrane peptide dimer2 in detergent micelles with his173-ser172 intermonomer3 hydrogen bond restraints 10 c2ka1B_ 15.2 33 PDB header:membrane protein Chain: B: PDB Molecule:bcl2/adenovirus e1b 19 kda protein-interacting PDBTitle: solution nmr structure of bnip3 transmembrane peptide dimer2 in detergent micelles 11 c2ka1A_ 15.2 33 PDB header:membrane protein Chain: A: PDB Molecule:bcl2/adenovirus e1b 19 kda protein-interacting PDBTitle: solution nmr structure of bnip3 transmembrane peptide dimer2 in detergent micelles 12 d2qwva1 15.0 25 Fold:alpha/beta knot Superfamily:alpha/beta knot Family:AF1056-like 13 d2qmma1 13.6 27 Fold:alpha/beta knot Superfamily:alpha/beta knot Family:AF1056-like 14 d1acca_ 13.1 16 Fold:Anthrax protective antigen Superfamily:Anthrax protective antigen Family:Anthrax protective antigen 15 d1ig8a1 11.7 60 Fold:Ribonuclease H-like motif Superfamily:Actin-like ATPase domain Family:Hexokinase 16 c2j5dA_ 11.2 32 PDB header:membrane protein Chain: A: PDB Molecule:bcl2/adenovirus e1b 19 kda protein-interacting PDBTitle: nmr structure of bnip3 transmembrane domain in lipid2 bicelles 17 d1fx8a_ 11.1 20 Fold:Aquaporin-like Superfamily:Aquaporin-like Family:Aquaporin-like 18 c1ldaA_ 11.1 20 PDB header:transport protein Chain: A: PDB Molecule:glycerol uptake facilitator protein; PDBTitle: crystal structure of the e. coli glycerol facilitator (glpf) without2 substrate glycerol 19 c3zy6A_ 11.1 29 PDB header:transferase Chain: A: PDB Molecule:putative gdp-fucose protein o-fucosyltransferase 1; PDBTitle: crystal structure of pofut1 in complex with gdp-fucose2 (crystal-form-ii) 20 d1czan1 10.8 50 Fold:Ribonuclease H-like motif Superfamily:Actin-like ATPase domain Family:Hexokinase 21 c2b2hA_ not modelled 10.2 26 PDB header:transport protein Chain: A: PDB Molecule:ammonium transporter; PDBTitle: ammonium transporter amt-1 from a. fulgidus (as) 22 c3op9A_ not modelled 10.0 14 PDB header:transcription regulator Chain: A: PDB Molecule:pli0006 protein; PDBTitle: crystal structure of transcriptional regulator from listeria innocua 23 d1czan3 not modelled 9.9 60 Fold:Ribonuclease H-like motif Superfamily:Actin-like ATPase domain Family:Hexokinase 24 d1bdga1 not modelled 9.3 60 Fold:Ribonuclease H-like motif Superfamily:Actin-like ATPase domain Family:Hexokinase 25 d1bg3a3 not modelled 9.2 60 Fold:Ribonuclease H-like motif Superfamily:Actin-like ATPase domain Family:Hexokinase 26 d1bg3a1 not modelled 9.1 50 Fold:Ribonuclease H-like motif Superfamily:Actin-like ATPase domain Family:Hexokinase 27 c2k3uA_ not modelled 8.8 37 PDB header:immune system Chain: A: PDB Molecule:chemotaxis inhibitory protein; PDBTitle: structure of the tyrosine-sulfated c5a receptor n-terminus2 in complex with the immune evasion protein chips. 28 c3llqB_ not modelled 8.8 19 PDB header:membrane protein Chain: B: PDB Molecule:aquaporin z 2; PDBTitle: aquaporin structure from plant pathogen agrobacterium tumerfaciens 29 d1v4sa1 not modelled 8.4 50 Fold:Ribonuclease H-like motif Superfamily:Actin-like ATPase domain Family:Hexokinase 30 c2lmfA_ not modelled 7.2 43 PDB header:antimicrobial protein Chain: A: PDB Molecule:antibacterial protein ll-37; PDBTitle: solution structure of human ll-23 bound to membrane-mimetic micelles 31 c3hm8D_ not modelled 7.0 60 PDB header:transferase Chain: D: PDB Molecule:hexokinase-3; PDBTitle: crystal structure of the c-terminal hexokinase domain of human hk3 32 c2w2eA_ not modelled 6.9 26 PDB header:membrane protein Chain: A: PDB Molecule:aquaporin; PDBTitle: 1.15 angstrom crystal structure of p.pastoris aquaporin,2 aqy1, in a closed conformation at ph 3.5 33 d1rc2a_ not modelled 6.9 13 Fold:Aquaporin-like Superfamily:Aquaporin-like Family:Aquaporin-like 34 c3p04B_ not modelled 6.6 14 PDB header:structural genomics, unknown function Chain: B: PDB Molecule:uncharacterized bcr; PDBTitle: crystal structure of the bcr protein from corynebacterium glutamicum.2 northeast structural genomics consortium target cgr8 35 c2magA_ not modelled 6.4 35 PDB header:antibiotic Chain: A: PDB Molecule:magainin 2; PDBTitle: nmr structure of magainin 2 in dpc micelles, 10 structures 36 c3c9jD_ not modelled 6.2 21 PDB header:membrane protein Chain: D: PDB Molecule:proton channel protein m2, transmembrane segment; PDBTitle: the crystal structure of transmembrane domain of m2 protein and2 amantadine complex 37 c3c9jA_ not modelled 6.2 21 PDB header:membrane protein Chain: A: PDB Molecule:proton channel protein m2, transmembrane segment; PDBTitle: the crystal structure of transmembrane domain of m2 protein and2 amantadine complex 38 c3c9jB_ not modelled 6.2 21 PDB header:membrane protein Chain: B: PDB Molecule:proton channel protein m2, transmembrane segment; PDBTitle: the crystal structure of transmembrane domain of m2 protein and2 amantadine complex 39 c3c9jC_ not modelled 6.2 21 PDB header:membrane protein Chain: C: PDB Molecule:proton channel protein m2, transmembrane segment; PDBTitle: the crystal structure of transmembrane domain of m2 protein and2 amantadine complex 40 c3ct5A_ not modelled 6.0 14 PDB header:hydrolase Chain: A: PDB Molecule:morphogenesis protein 1; PDBTitle: crystal and cryoem structural studies of a cell wall degrading enzyme2 in the bacteriophage phi29 tail 41 d1u9ya2 not modelled 6.0 38 Fold:PRTase-like Superfamily:PRTase-like Family:Phosphoribosylpyrophosphate synthetase-like 42 d1u7ga_ not modelled 5.9 16 Fold:Ammonium transporter Superfamily:Ammonium transporter Family:Ammonium transporter 43 d1wi9a_ not modelled 5.7 63 Fold:DNA/RNA-binding 3-helical bundle Superfamily:"Winged helix" DNA-binding domain Family:PCI domain (PINT motif) 44 c1ymgA_ not modelled 5.7 26 PDB header:membrane protein Chain: A: PDB Molecule:lens fiber major intrinsic protein; PDBTitle: the channel architecture of aquaporin o at 2.2 angstrom resolution 45 d1ymga1 not modelled 5.7 26 Fold:Aquaporin-like Superfamily:Aquaporin-like Family:Aquaporin-like 46 d1l8wd_ not modelled 5.6 35 Fold:Variable surface antigen VlsE Superfamily:Variable surface antigen VlsE Family:Variable surface antigen VlsE 47 c3c1iA_ not modelled 5.5 20 PDB header:transport protein Chain: A: PDB Molecule:ammonia channel; PDBTitle: substrate binding, deprotonation and selectivity at the2 periplasmic entrance of the e. coli ammonia channel amtb 48 c1iyjC_ not modelled 5.5 55 PDB header:gene regulation/antitumor protein Chain: C: PDB Molecule:deleted in split hand/split foot protein 1; PDBTitle: structure of a brca2-dss1 complex 49 c1bdgA_ not modelled 5.5 60 PDB header:hexokinase Chain: A: PDB Molecule:hexokinase; PDBTitle: hexokinase from schistosoma mansoni complexed with glucose 50 c1v4sA_ not modelled 5.5 50 PDB header:transferase Chain: A: PDB Molecule:glucokinase isoform 2; PDBTitle: crystal structure of human glucokinase 51 c1ig8A_ not modelled 5.4 60 PDB header:transferase Chain: A: PDB Molecule:hexokinase pii; PDBTitle: crystal structure of yeast hexokinase pii with the correct2 amino acid sequence 52 c2oarA_ not modelled 5.3 26 PDB header:membrane protein Chain: A: PDB Molecule:large-conductance mechanosensitive channel; PDBTitle: mechanosensitive channel of large conductance (mscl) 53 c3hzqA_ not modelled 5.3 26 PDB header:membrane protein Chain: A: PDB Molecule:large-conductance mechanosensitive channel; PDBTitle: structure of a tetrameric mscl in an expanded intermediate2 state 54 c3pvpA_ not modelled 5.1 10 PDB header:dna binding protein/dna Chain: A: PDB Molecule:chromosomal replication initiator protein dnaa; PDBTitle: structure of mycobacterium tuberculosis dnaa-dbd in complex with box22 dna

Binding site prediction

Due to computational demand, binding site predictions are not run for batch jobs

If you want to predict binding sites, please manually submit your model of choice to 3DLigandSite

---

Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
If you use the binding site predictions from 3DLigandSite, please also cite:
3DLigandSite: predicting ligand-binding sites using similar structures.
Wass MN, Kelley LA and Sternberg MJ Nucleic Acids Research 38, W469-73 (2010) [PubMed]
© Structural Bioinformatics Group Imperial College London Lawrence Kelley, Benjamin Jefferys Disclaimer Terms and Conditions	Component software Template detection: HHpred 1.51 Secondary structure prediction: Psi-pred 2.5 Disorder prediction: Disopred 2.4 Transmembrane prediction: Memsat_SVM Multi-template modelling and ab initio: Poing 1.0