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Job DescriptionP15067
Confidence100.00%DateThu Jan 5 11:34:29 GMT 2012
Rank9Aligned Residues586
% Identity25%Templatec2ya1A_
PDB info PDB header:hydrolaseChain: A: PDB Molecule:putative alkaline amylopullulanase; PDBTitle: product complex of a multi-modular glycogen-degrading2 pneumococcal virulence factor spua
Resolution2.25 Å

  Insertion relative to template
  Deletion relative to template
  Catalytic residue from the CSA
 
Detailed help on interpreting your alignment


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Query Sequence  PLGAHYDGQG. . VNFTLFSAHAERVELCVFDANGQE. . . HRYDLPGHSGDIWHGYLPDARP. . . . . . . GLRYGYRVHGPW
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Template Sequence  KLGADLKEEGKQVDLTLWSPSADKVSVVVYDKNDPDKVVGTVALEKGERGTWKQTLDSTNKLGITDFTGYYYQYQIE. . .
Template Known Secondary structure 

GGGT
TT
S
SS
TTS

GGG
TT
SS

S

TT
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   450.........460.........470.........480.........490.........500.........510.........520......
 
   7980.........90.........100.........110.........120.........130.........140....... ..150....
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Query Sequence  QPAEGHRFNPAKLLIDPCARQIDGEFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTP. . . . WGSTIIY
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Template Sequence  . . . . . . RQGKTVLALDPYAKSLAAWNSDDSKI. . . . . . . . . DDAHKVAKAAFVDPAKLGPQDLTYGKIHNFKTREDAVIY
Template Known Secondary structure  ......TT

TT
SSB


BGGGTTS.........
GGGSS




GGGSS
TT





TT
SSGGG

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   527..530.........540.........550.. .......560.........570.........580.........590.
 
   155....160......... 170.........180.........190.........200.........210... ......220...
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Query Sequence  EAHVKGLTYLHPEIP. . VEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRM. . . . . . . . . GLSNYWGYNP
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Template Sequence  EAHVRDFTSDPAIAKDLTKPFGTFEAF. . IEKLDYLKDLGVTHIQLLPVLSYYFVNELKNHERLSDYASSNSNYNWGYDP
Template Known Secondary structure 


GGGTTT
SS
TTS..TTT
SS

B
BSS
BGGGTTS


S

SSS
S


S
SB
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   592.......600.........610........ .620.........630.........640.........650.........660.........
 
   224.....230......... 240.........250.........260.........270.........280.........290.........300
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Query Sequence  VAMFALHPAYACSPET. . . ALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPLFSLRGIDNRSYYWIREDGDYHNWT
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Template Sequence  QNYFSLTGMYSSDPKNPEKRIAEFKNLINEIHKRGMGAILDVVYNHTAKVDL. . . . . . FEDLEPNYYHFMDADGTPRTSF
Template Known Secondary structure  S
SSSB
STT
S
TT
TTTTT

TT

S
......TTSTTTSB
B
TT

B
T
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   670.........680.........690.........700.........710.........720. ........730.........740...
 
   301........310.........320.........330.........340.........350.........360.........370.....
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Query Sequence  GCGNTLNLSHPAVVDYASACLRYWVETCHVDGFRFDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDI. . . . .
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Template Sequence  GG. GRLGTTHHMTKRLLIDSIKYLVDTYKVDGFRFDMMGDHDAASIEEAYKAARA. . . . . . LNPNLIMLGEGWRTYAGDE
Template Known Secondary structure  T.
B
TTS


TTGGGSB......
TT



S



ST
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   744. ....750.........760.........770.........780.........790....... ..800.........810......
 
   376...380.........390.........400.. .......410.........420.........430.........440..
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Query Sequence  . . . . APGGYQVGNFPPLFAEWNDHFRDAARR. . . . . . . . . FWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAH
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Template Sequence  NMPTKAADQDWMKHTDTVAVFSDDIRNNLKSGYPNEGQPAFITGGKRDVNTIFKNLIAQPTNFEADS. . PGDVIQYIAAH
Template Known Secondary structure  T



BSSGGGGGG
SSS
S

TTSTT


TTTT

TT

SSS

SS..GGG
S

S
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   817..820.........830.........840.........850.........860.........870.........880... ......890....
 
   443......450.........460.........470.........480.........490.........500.........510.........520..
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Query Sequence  DGFTLRDCVCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGSLDLVERRRDSIHALLTTLLLSQGTPMLLAGDEHGHSQ
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Template Sequence  DNLTLFDIIAQSIKKDPSKAENYAEI. . . . . . . . . . . . . . . . . . . . . . . . HRRLRLGNLMVLTAQGTPFIHSGQEYGRTK
Template Known Secondary structure  SSS
T

TTS........................TSSSTTTTTT


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   895....900.........910.........920 .........930.........940.........950
 
   523... ...530.........540... ......550.........560
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Query Sequence  HGNN. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . NAYCQDNQLTWLDWSQA. . . . . . . . SSGLTAFTAALIHLRKR
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Template Sequence  QFRNPAYRTPVAEDKVPNKSHLLRDKDGNPFDYPYFIHDSYDSSDAVNKFDWTKATDGKAYPENVKSRDYMKGLIALRQS
Template Known Secondary structure  B


GGGSS


TTT

TT
TTS
S

TT





TTT
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   951........960.........970.........980.........990.........1000.........1010.........1020.........1030
 
   561........570.........580.........590.........600.........610.........620.... .....630.....
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Query Sequence  IPALVENRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA. . . . . GEWHAIPPFAG
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Template Sequence  TDAFRLKS. LQDIKDRVHLITVPGQNGVEKEDVVIGYQITAPNGDIYAVFVNADEKAREFNLGTAFAHLRNAEVLADENQ
Template Known Secondary structure 
GGGS
S
.SS
TTSTT

SS
TTS

SSS

TTTTGGGGG

SS
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   1031....... .1040.........1050.........1060.........1070.........1080.........1090.........1100.........
 
   636...640. ........650......
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Query Sequence  EDNPVI. . . . . . . . . TAVWQGPAHGLCVFQ
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Template Sequence  AGPVGIANPKGLEWTEKGLKLNALTATVLR
Template Known Secondary structure  B
SS

SS
SS
SS
TT
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   1110.........1120.........1130.........
 
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Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
 
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Lawrence Kelley, Benjamin Jefferys 
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