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Job DescriptionP15067
Confidence100.00%DateThu Jan 5 11:34:29 GMT 2012
Rank29Aligned Residues424
% Identity20%Templatec2wcsA_
PDB info PDB header:hydrolaseChain: A: PDB Molecule:alpha amylase, catalytic region; PDBTitle: crystal structure of debranching enzyme from nostoc2 punctiforme (npde)
Resolution2.80 Å

  Insertion relative to template
  Deletion relative to template
  Catalytic residue from the CSA
 
Detailed help on interpreting your alignment


   142...... .150.........160.........170 .........180.........190.........200..
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Query Sequence  APPRTPW. GSTIIYEAHVKGLTYLHPEIPV. . . . . . . . . . . . . . . . . . EIRGTYKALGHPVMINYLKQLGITALELLPVA
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Template Sequence  QIQTPDWVKHAVFYQIFPDRFARSKQPRKRLLQEARWEDWDSMPTLQGYKGGDLWGI. . MEDLDYIQNLGINAIYFTPIF
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TTGGG


GGGT


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S
TT

TTTGGG
T

S

..TT

S

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   2.......10.........20.........30.........40.........50........ .60.........70.........
 
   203......210.........220.........230.........240.........250.........260.........270.........280..
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Query Sequence  QFASEPRLQRMGLSNYWGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPLFSLRGI
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Template Sequence  QSAS. . . . . . . . . . . NHRYHTHDYYQVDPMLGG. . . . . NEAFKELLDAAHQRNIKVVLDGVFNHSSRGFFFFHDVLENGP
Template Known Secondary structure  ST...........T
TTS
GGGT.....TT


SB

TTSGG
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   80... ......90.........100. ........110.........120.........130.........140...
 
   283......290.........300.. .......310.........320.........330.........340... ....
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Query Sequence  DNRSYYWIREDGDYHNWTGC. . . . . . . . . . . . . . GNTLNLSHPAVVDYASACLRYWVETCHVDGFRFDLAAVMGR. TPEF
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Template Sequence  HSPWVNWFKIEGWPLSPYNGEFPANYVGWAGNRALPEFNHDNPEVREYIMEIAEYWL. KFGIDGWRLDVPFEIKTPGFWQ
Template Known Secondary structure  G
TTGGGB
B
SSS

SS

SS

SB
BGGG
TTSBBB
TTS.T

STTS


TT
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   144.....150.........160.........170.........180.........190.........200 .........210.........220..
 
   348.350.........360.........370.........380.........390.........400....... ..410.
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Query Sequence  RQDAPLFTAIQNCPVLSQVKLIAEPWDIAPGGYQVGNFPPLFAEWNDHFRDAARRFWLHY. . . . . . . . . . . . . . . . DLPL
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Template Sequence  EFRDRTKA. . . . . . INPEAYIVGEVWGDSRQWLDGTQFD. . . GVMNYLFAGPTIAFAAGDRVVLEQVQSRDYQPYPPLFA
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TT


S

GGG
SSSS
S...TGGG

SSS

TTT



S


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   223......230 .........240.........250..... ....260.........270.........280.........290...
 
   412.......420.........430.........440.........450.........460.........470.........480.........490.
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Query Sequence  GAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDCVCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGSLDLVER
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Template Sequence  AEYATKIQEVLQLYPWEI. . QLTQLNLLASHDTARLMTIAGGD. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Template Known Secondary structure  TSS
..TT

S

TTS

TTT
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   294.....300.........310. ........320.........330....
 
   492.......500.........510.........520.........530.........540.........550.........560.........570.
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Query Sequence  RRDSIHALLTTLLLSQGTPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRIPALVENRWWE
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Template Sequence  . IASVELSTLLLLTFPGAPSIYYGDEVGLPGGID. . . . . . PDSRRGFPEANWNQEIFNTHRQLITIRQTYPALRTGD. . .
Template Known Secondary structure  .TTSSSTTGGGT



SS......SSS










TS...
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   335....340.........350.........360....... ..370.........380.........390.........400....
 
   572.......580.........590.........600.........610.........620... ......630.... .....640..
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Query Sequence  EGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLP. . . . AGEWHAIPPFA. . . . . GEDNPVIT
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Template Sequence  . . . . . . . . . . . . . . YQVLYAQGQLYLFARTLGTEELIIAINAGTSSATANVDVASLHTQPNKLLYGTAEAEWNGEEGTQQ
Template Known Secondary structure  ..............TTTT
SSS



S
SS


S




STT

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   406...410.........420.........430.........440.........450.........460.........470.
 
   643......650......
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Query Sequence  AVWQGPAHGLCVFQ
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Template Sequence  LSLTLPARSGCILG
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SS
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   472.......480.....
 
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No model constructed - rank, confidence too low




Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
 
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Lawrence Kelley, Benjamin Jefferys 
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