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Job DescriptionP15067
Confidence100.00%DateThu Jan 5 11:34:29 GMT 2012
Rank4Aligned Residues603
% Identity24%Templatec2fhfA_
PDB info PDB header:hydrolaseChain: A: PDB Molecule:pullulanase; PDBTitle: crystal structure analysis of klebsiella pneumoniae2 pullulanase complexed with maltotetraose
Resolution1.65 Å

  Insertion relative to template
  Deletion relative to template
  Catalytic residue from the CSA
 
Detailed help on interpreting your alignment


   6...10.........20.........30.........40........ .50.........60.........70.........80...
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Query Sequence  IGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQEHRYD. . LPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEG
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Template Sequence  AAEALSYGAQLTDSGVTFRVWAPTAQQVELVIYSADKKVIASHPMTRDSASGAWSWQGGSDLKGAFYRYAMT. VYHPQSR
Template Known Secondary structure  TS

TT
TT
S
TT



TTT

GGGTT
.TTTT
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   291........300.........310.........320.........330.........340.........350.........360.. .......
 
   84.....90.........100.........110.........120.........130.........140.........150.........160...
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Query Sequence  HRFNPAKLLIDPCARQIDGEFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKGLTY
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Template Sequence  KV. . EQYEVTDPYAHSLSTNSEYSQVVDLNDSALKPEGWDGL. . . . . . . . . . . TMPHAQKTKADLAKMTIHESHIRDLSA
Template Known Secondary structure  ..

TT
S
B
GGGSS
B
TT
GGGS
TTGGG
...........

SS

SST

T
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   370. ........380.........390.........400......... 410.........420.........430......
 
   164.....170.........180. ........190.........200.........210.....
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Query Sequence  LHPEIPVEIRGTYKALGH. . . . . PVMINYLKQLGITALELLPVAQFASEPRLQRMGL. . . . . . . . . . . . . . . . . . . . . . .
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Template Sequence  WDQTVPAELRGKYLALTAQESNMVQHLKQLSASGVTHIELLPVFDLATVNEFSDKVADIQQPFSRLCEVNSAVKSSEFAG
Template Known Secondary structure  T
TTS
GGGTTSGGGGG
TTST

S

SSS


GGG


TTSB
TSTTGG
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   437..440.........450.........460.........470.........480.........490.........500.........510......
 
   216...220.........230......... 240.........250... ..
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Query Sequence  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . SNYWGYNPVAMFALHPAYACSPET. . ALDEFRDAIKALHK. AG
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Template Sequence  YCDSGSTVEEVLTQLKQNDSKDNPQVQALNTLVAQTDSYNWGYDPFHYTVPEGSYATDPEGTARIKEFRTMIQAIKQDLG
Template Known Secondary structure  GTTSS
BSTT
BTTB
TTSSS






STTSS

STTTS
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   517..520.........530.........540.........550.........560.........570.........580.........590......
 
   256...260.........270.........280.........290.........300.........310.........320.........330.....
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Query Sequence  IEVILDIVLNHSAELDLDGPLFSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFRF
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Template Sequence  MNVIMDVVYNHTNAAGPTDRTSVLDKIVPWYYQRLNETTGSVESATCCSDSAPEHRMFAKLIADSLAVWTTDYKIDGFRF
Template Known Secondary structure 

TTS

SSS
TT

STTTSB
B
TTT

B

TTSSB
TTS


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   597..600.........610.........620.........630.........640.........650.........660.........670......
 
   336...340.........350.........360.........370......... 380.........390.........400.........410
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Query Sequence  DLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPGG. . . . . YQVGNFPPLFAEWNDHFRDAARRFWLHYDLP
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Template Sequence  DLMLYHPKAQILSAWERIKA. . . . . . LNPDIYFFGEGWDSNQSDRFEIASQINLKGTGIGTFSDRLRDAVRGGGPFDSGD
Template Known Secondary structure  TTGGGSBT......T
TT





S
TTTS
B

TTTTTTSS


S
TT

TT
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   677..680.........690...... ...700.........710.........720.........730.........740.........750
 
   411........420........ .430.........
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Query Sequence  LGAFAGRFAASSDVFKRN. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . GRLPSAAINLV
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Template Sequence  ALRQNQGVGSGAGVLPNELTTLSDDQARHLADLTRLGMAGNLADFVLIDKDGAVKRGSEIDYNGAPGGYAADPTEVVNYV
Template Known Secondary structure 


GGGTTTTS

SS



TT
BTT

TTS
GGGSTTS

BSSGGG
S
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   751........760.........770.........780.........790.........800.........810.........820.........830
 
   440.........450.........460.........470.........480.........490.........500.........510.........
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Query Sequence  TAHDGFTLRDCVCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGSLDLVERRRDSIHALLTTLLLSQGTPMLLAGDEHG
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Template Sequence  SKHDNQTLWDMISYK. . . . . . . . . . . . . . . . . . . . . . . . . . . . AAQEADLDTRVRMQAVSLATVMLGQGIAFDQQGSELL
Template Known Secondary structure 

SSSS
............................S
TT

TSSSTTGGGT
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   831........840..... ....850.........860.........870.........880..
 
   520.........530.........540..... ....550.........560....
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Query Sequence  HSQHGNNNAYCQDNQLTWLDWSQASS. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . GLTAFTAALIHLRKRIPAL
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Template Sequence  RSKSFTRDSYDSGDWFNRVDYSLQDNNYNVGMPRSSDDGSNYDIIARVKDAVATPGETELKQMTAFYQELTALRKSSPLF
Template Known Secondary structure 


TT
S

TTS


TT
S

S
SSS


GGGTTSS



TS
GGG
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   883......890.........900.........910.........920.........930.........940.........950.........960..
 
   565....570.........580.. .......590.........600.........610.........620.........630....
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Query Sequence  VENRWWEEGDGNVRWLNR. . . . . YAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPAGEWHAIPPFA. . . . .
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Template Sequence  TLGD. GATVMKRVDFRNTGADQQTGLLVMTIDDGMQAGASLDSRVDGIVVAINAAPESRTLQDFAGTSLQLSAIQQAAGD
Template Known Secondary structure  G


.S
STT

TT
STTTSS
S
TT
SSS

TTSSS


TGG
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   963... ...970.........980.........990.........1000.........1010.........1020.........1030.........1040.
 
   635....640.........650.......
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Query Sequence  . . . . GEDNPVITAVWQGPAHGLCVFQR
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Template Sequence  RSLASGVQVAADGSVTLPAWSVAVLEL
Template Known Secondary structure  GSTTTT

TTS

TT
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   1042.......1050.........1060........
 
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Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
 
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Lawrence Kelley, Benjamin Jefferys 
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