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Job DescriptionP15067
Confidence100.00%DateThu Jan 5 11:34:29 GMT 2012
Rank21Aligned Residues528
% Identity20%Templatec2d0gA_
PDB info PDB header:hydrolaseChain: A: PDB Molecule:alpha-amylase i; PDBTitle: crystal structure of thermoactinomyces vulgaris r-47 alpha-2 amylase 1 (tvai) mutant d356n/e396q complexed with p5, a3 pullulan model oligosaccharide
Resolution2.60 Å

  Insertion relative to template
  Deletion relative to template
  Catalytic residue from the CSA
 
Detailed help on interpreting your alignment


   11........20.........30.........40 .........50.... .....60.........70.........80...
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Query Sequence  PLGAHYDGQGVNFTLFSAHAERVELCVFDA. NGQEHRYDLPGHSG. . . . . . DIWHGYLPDARPGLRYGYRVHGPWQPAEG
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Template Sequence  PEPTSTQSVTLKLRTFKGDITSANIKYWDTADNAFHWVPMVWDSNDPTGTFDYWKGTIPASPSIKYYRFQIN. . . . . . . .
Template Known Secondary structure  SS

TT

TT

STTTT

TTSS


SS
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   24.....30.........40.........50.........60.........70.........80.........90.....
 
   84.....90.........100.........110.........120.........130.........140.........150.........160...
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Query Sequence  HRFNPAKLLIDPCARQIDGEFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKGLTY
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Template Sequence  . DGTSTAWYNGNGPSSTEP. NADDFYIIPNFKTPDW. . . . . . . . . . . . . . . . . . . . . . . . . . . . LKNGVMYQIFPDRFYN
Template Known Secondary structure  .TTTTSS

.SS


TT



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GGGT

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   96...100.........110... ......120......... 130.........140.....
 
   164.....170... ......180.........190 .........200.........210
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Query Sequence  LHPEIPVEIR. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . GTYKALGHPVMINYLKQ. LGITALELLPVAQFASEPRL
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Template Sequence  GDSSNDVQTGSYTYNGTPTEKKAWGSSVYADPGYDNSLVFFGGDLAGI. . DQKLGYIKKTLGANILYLNPIFKAPT. . . .
Template Known Secondary structure  S
GGG


TTS
BSSS
B


TTS

S

TTS
GGG



..TTTT


S


SS....
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   146...150.........160.........170.........180.........190... ......200.........210.........
 
   211........220.........230.........240.........250..... ....260.........270......
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Query Sequence  QRMGLSNYWGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAG. . . . IEVILDIVLNHSAELDLDGPL. . . . . . . . . .
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Template Sequence  . . . . . . . NHKYDTQDYMAVDPAFGD. . . . . NSTLQTLINDIHSTANGPKGYLILDGVFNHTGDSHPWFDKYNNFSSQGAY
Template Known Secondary structure  .......SS

S
S
TTT

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SSSSS



SB

TTSTTT
TT

SSS

TT
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   220.........230....... ..240.........250.........260.........270.........280.......
 
   277..280.........290.........300.. .......310 .........320.. .......330.........340.......
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Query Sequence  FSLRGIDNRSYYWIREDGDYHNWTGC. . GNTLNLSH. . PAVVDYASACLR. . . . . YWVETCHVDGFRFDLAAVMGRTPEF
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Template Sequence  ESQSSPWYNYYTFYTWPDSYASFLGFNSLPKLNYGNSGSAVRGVIYNNSNSVAKTYLNPPYSVDGWRLNAAQYVDANGNN
Template Known Secondary structure  T
TT
TTGGGB
TTT
TTBB


STT
TTSTTSSTTT


TTGGG

STT

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   288.290.........300.........310.........320.........330.........340.........350.........360.......
 
   348.350.. ....... 360.........370.........380.........390... ......400....... ..410....
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Query Sequence  RQDAP. . . LFTAIQN. . CPVLSQVKLIAEPWDIAPGGYQVGNFPPLFAEWN. DHFRDAARRFWLHY. . . . . . . DLPLGAF
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Template Sequence  GSDVTNHQIWSEFRNAVKGVNSNAAIIGQYWGNANPWTAQG. . NQWDAATNFDGFTQPVSEWITGKDYQNNSASISTTQF
Template Known Secondary structure 
SS
TT


SS

GGGTTTS..SS
SB
TTTTSB
TTS
B



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   368.370.........380.........390.........400........ .410.........420.........430.........440.....
 
   415....420.........430.........440.........450.........460.........470.........480.........490....
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Query Sequence  AGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDCVCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGSLDLVERRRD
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Template Sequence  DSWLRGTRANYPTNV. . QQSMMNFLSNHDITRFATRSGGD. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . LW
Template Known Secondary structure  TTS
..TT
S

TTS

TTT
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   446...450.........460 .........470.........480... ..
 
   495....500.........510.........520.........530.........540... ......550.........560.........570..
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Query Sequence  SIHALLTTLLLSQGTPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQA. . SSGLTAFTAALIHLRKRIPALVENRWWEE
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Template Sequence  KTYLALIFQMTYVGTPTIYYGDEYGMQGGAD. . . . . . PDNRRSFDWSQATPSNSAVALTQKLITIRNQYPALRTGSFM. .
Template Known Secondary structure  TTSSSTTTTTT



ST......TTTS



TTTSSTTS
S..
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   486...490.........500.........510...... ...520.........530.........540.........550.......
 
   573......580.........590.........600.........610.........620...... ...630.........640....
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Query Sequence  GDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPAGE. . . . . . . . WHAIPPFAGEDNPVITAV
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Template Sequence  . . . . . . . . . . . . . TLITDDTNKIYSYGRFDNVNRIAVVLNNDSVSHTVNVPVWQLSMPNGSTVTDKITGHSYTVQNGMVT
Template Known Secondary structure  .............TTTT
SS

SSS

GGGGT

TT
TTT


BTTB
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   558.560.........570.........580.........590.........600.........610.........620....
 
   645....650.......
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Query Sequence  WQGPAHGLCVFQR
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Template Sequence  VAVDGHYGAVLAQ
Template Known Secondary structure 
TT
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   625....630.......
 
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No model constructed - rank, confidence too low




Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
 
© Structural Bioinformatics Group, Imperial College, London
Lawrence Kelley, Benjamin Jefferys 
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