Secondary structure and disorder prediction

		1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	K	T	I	L	P	A	V	L	F	A	A	F	A	T	T	S	A	W	A	A	E	S	V	Q	P	L	E	K	I	A	P	Y	P	Q	A	E	K	G	M	K	R	Q	V	I	Q	L	T	P	Q	E	D	E	S	T	L	K	V	E	L
Secondary structure
SS confidence
Disorder		?	?	?	?									?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?			?																	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		L	I	G	Q	T	L	E	V	D	C	N	L	H	R	L	G	G	K	L	E	N	K	T	L	E	G	W	G	Y	D	Y	Y	V	F	D	K	V	S	S	P	V	S	T	M	M	A	C	P	D	G	K	K	E	K	K	F	V	T	A	Y
Secondary structure
SS confidence
Disorder																						?	?														?	?	?	?	?	?	?	?	?	?	?	?	?	?	?		?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.
Sequence		L	G	D	A	G	M	L	R	Y	N	S	K	L	P	I	V	V	Y	T	P	D	N	V	D	V	K	Y	R	V	W	K	A	E	E	K	I	D	N	A	V	V	R
Secondary structure
SS confidence
Disorder									?																								?	?	?	?					?	?	?
Disorder confidence

Confidence Key
High(9)											Low (0)

?	Disordered
	Alpha helix
	Beta strand

Domain analysis

Hover over an aligned region to see model and summary info

Please note, only up to the top 20 hits are modelled to reduce computer load

Rank Aligned region

PDB 1xxf chain C Region: 25 - 162 Aligned: 138 Modelled: 138 Confidence: 100.0% Identity: 96% Fold: Ecotin, trypsin inhibitor Superfamily: Ecotin, trypsin inhibitor Family: Ecotin, trypsin inhibitor Phyre2 PDB 2y6t chain E Region: 24 - 162 Aligned: 139 Modelled: 139 Confidence: 100.0% Identity: 70% PDB header:hydrolase/inhibitor Chain: E: PDB Molecule:ecotin; PDBTitle: molecular recognition of chymotrypsin by the serine2 protease inhibitor ecotin from yersinia pestis Phyre2 PDB 1slu chain A Region: 30 - 162 Aligned: 131 Modelled: 133 Confidence: 100.0% Identity: 94% Fold: Ecotin, trypsin inhibitor Superfamily: Ecotin, trypsin inhibitor Family: Ecotin, trypsin inhibitor Phyre2 PDB 1ezs chain A Region: 32 - 162 Aligned: 125 Modelled: 131 Confidence: 100.0% Identity: 98% Fold: Ecotin, trypsin inhibitor Superfamily: Ecotin, trypsin inhibitor Family: Ecotin, trypsin inhibitor Phyre2 PDB 1fi8 chain E Region: 26 - 101 Aligned: 75 Modelled: 76 Confidence: 100.0% Identity: 92% PDB header:hydrolase/hydrolase inhibitor Chain: E: PDB Molecule:ecotin; PDBTitle: rat granzyme b [n66q] complexed to ecotin [81-84 iepd] Phyre2 PDB 1fi8 chain F Region: 115 - 162 Aligned: 48 Modelled: 48 Confidence: 99.7% Identity: 100% PDB header:hydrolase/hydrolase inhibitor Chain: F: PDB Molecule:ecotin; PDBTitle: rat granzyme b [n66q] complexed to ecotin [81-84 iepd] Phyre2 PDB 2la7 chain A Region: 70 - 110 Aligned: 30 Modelled: 41 Confidence: 90.2% Identity: 27% PDB header:structural genomics, unknown function Chain: A: PDB Molecule:uncharacterized protein; PDBTitle: nmr structure of the protein yp_557733.1 from burkholderia xenovorans Phyre2 PDB 2kts chain A Region: 70 - 109 Aligned: 29 Modelled: 40 Confidence: 82.4% Identity: 34% PDB header:chaperone Chain: A: PDB Molecule:heat shock protein hslj; PDBTitle: nmr structure of the protein np_415897.1 Phyre2 PDB 1uw7 chain A Region: 59 - 147 Aligned: 84 Modelled: 84 Confidence: 29.8% Identity: 19% PDB header:replicase protein Chain: A: PDB Molecule:nsp9; PDBTitle: nsp9 protein from sars-coronavirus. Phyre2 PDB 2bhu chain A domain 1 Region: 137 - 155 Aligned: 19 Modelled: 19 Confidence: 25.5% Identity: 16% Fold: Immunoglobulin-like beta-sandwich Superfamily: E set domains Family: E-set domains of sugar-utilizing enzymes Phyre2 PDB 1qz8 chain A Region: 59 - 147 Aligned: 84 Modelled: 89 Confidence: 22.2% Identity: 19% Fold: Replicase NSP9 Superfamily: Replicase NSP9 Family: Replicase NSP9 Phyre2 PDB 3ff6 chain D Region: 130 - 146 Aligned: 17 Modelled: 17 Confidence: 19.0% Identity: 29% PDB header:ligase Chain: D: PDB Molecule:acetyl-coa carboxylase 2; PDBTitle: human acc2 ct domain with cp-640186 Phyre2 PDB 1od4 chain C Region: 130 - 146 Aligned: 17 Modelled: 17 Confidence: 18.4% Identity: 29% PDB header:ligase Chain: C: PDB Molecule:acetyl-coenzyme a carboxylase; PDBTitle: acetyl-coa carboxylase carboxyltransferase domain Phyre2 PDB 1gl0 chain I Region: 62 - 74 Aligned: 13 Modelled: 13 Confidence: 18.4% Identity: 38% Fold: PMP inhibitors Superfamily: PMP inhibitors Family: PMP inhibitors Phyre2 PDB 1wo9 chain A Region: 62 - 74 Aligned: 13 Modelled: 13 Confidence: 16.6% Identity: 46% Fold: PMP inhibitors Superfamily: PMP inhibitors Family: PMP inhibitors Phyre2 PDB 2qyq chain A domain 1 Region: 28 - 47 Aligned: 20 Modelled: 20 Confidence: 16.6% Identity: 20% Fold: PEBP-like Superfamily: PEBP-like Family: Phosphatidylethanolamine binding protein Phyre2 PDB 2gzq chain A Region: 30 - 47 Aligned: 18 Modelled: 18 Confidence: 16.4% Identity: 22% PDB header:lipid binding protein Chain: A: PDB Molecule:phosphatidylethanolamine-binding protein; PDBTitle: phosphatidylethanolamine-binding protein from plasmodium vivax Phyre2 PDB 2f91 chain B Region: 62 - 74 Aligned: 13 Modelled: 13 Confidence: 16.3% Identity: 46% PDB header:hydrolase/hydrolase inhibitor Chain: B: PDB Molecule:serine protease inhibitor i/ii; PDBTitle: 1.2a resolution structure of a crayfish trypsin complexed2 with a peptide inhibitor, sgti Phyre2 PDB 1uyr chain A domain 2 Region: 130 - 146 Aligned: 17 Modelled: 17 Confidence: 15.7% Identity: 29% Fold: ClpP/crotonase Superfamily: ClpP/crotonase Family: Biotin dependent carboxylase carboxyltransferase domain Phyre2 PDB 1wkp chain A Region: 34 - 47 Aligned: 14 Modelled: 14 Confidence: 15.2% Identity: 36% PDB header:signaling protein Chain: A: PDB Molecule:flowering locus t protein; PDBTitle: flowering locus t (ft) from arabidopsis thaliana Phyre2 1 d1xxfc_ 2 c2y6tE_ 3 d1slua_ 4 d1ezsa_ 5 c1fi8E_ 6 c1fi8F_ 7 c2la7A_ 8 c2ktsA_ 9 c1uw7A_ 10 d2bhua1 11 d1qz8a_ 12 c3ff6D_ 13 c1od4C_ 14 d1gl0i_ 15 d1wo9a_ 16 d2qyqa1 17 c2gzqA_ 18 c2f91B_ 19 d1uyra2 20 c1wkpA_ 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52

Detailed template information

# Template Alignment Coverage 3D Model Confidence % i.d. Template Information

1 d1xxfc_ 100.0 96 Fold:Ecotin, trypsin inhibitor Superfamily:Ecotin, trypsin inhibitor Family:Ecotin, trypsin inhibitor 2 c2y6tE_ 100.0 70 PDB header:hydrolase/inhibitor Chain: E: PDB Molecule:ecotin; PDBTitle: molecular recognition of chymotrypsin by the serine2 protease inhibitor ecotin from yersinia pestis 3 d1slua_ 100.0 94 Fold:Ecotin, trypsin inhibitor Superfamily:Ecotin, trypsin inhibitor Family:Ecotin, trypsin inhibitor 4 d1ezsa_ 100.0 98 Fold:Ecotin, trypsin inhibitor Superfamily:Ecotin, trypsin inhibitor Family:Ecotin, trypsin inhibitor 5 c1fi8E_ 100.0 92 PDB header:hydrolase/hydrolase inhibitor Chain: E: PDB Molecule:ecotin; PDBTitle: rat granzyme b [n66q] complexed to ecotin [81-84 iepd] 6 c1fi8F_ 99.7 100 PDB header:hydrolase/hydrolase inhibitor Chain: F: PDB Molecule:ecotin; PDBTitle: rat granzyme b [n66q] complexed to ecotin [81-84 iepd] 7 c2la7A_ 90.2 27 PDB header:structural genomics, unknown function Chain: A: PDB Molecule:uncharacterized protein; PDBTitle: nmr structure of the protein yp_557733.1 from burkholderia xenovorans 8 c2ktsA_ 82.4 34 PDB header:chaperone Chain: A: PDB Molecule:heat shock protein hslj; PDBTitle: nmr structure of the protein np_415897.1 9 c1uw7A_ 29.8 19 PDB header:replicase protein Chain: A: PDB Molecule:nsp9; PDBTitle: nsp9 protein from sars-coronavirus. 10 d2bhua1 25.5 16 Fold:Immunoglobulin-like beta-sandwich Superfamily:E set domains Family:E-set domains of sugar-utilizing enzymes 11 d1qz8a_ 22.2 19 Fold:Replicase NSP9 Superfamily:Replicase NSP9 Family:Replicase NSP9 12 c3ff6D_ 19.0 29 PDB header:ligase Chain: D: PDB Molecule:acetyl-coa carboxylase 2; PDBTitle: human acc2 ct domain with cp-640186 13 c1od4C_ 18.4 29 PDB header:ligase Chain: C: PDB Molecule:acetyl-coenzyme a carboxylase; PDBTitle: acetyl-coa carboxylase carboxyltransferase domain 14 d1gl0i_ 18.4 38 Fold:PMP inhibitors Superfamily:PMP inhibitors Family:PMP inhibitors 15 d1wo9a_ 16.6 46 Fold:PMP inhibitors Superfamily:PMP inhibitors Family:PMP inhibitors 16 d2qyqa1 16.6 20 Fold:PEBP-like Superfamily:PEBP-like Family:Phosphatidylethanolamine binding protein 17 c2gzqA_ 16.4 22 PDB header:lipid binding protein Chain: A: PDB Molecule:phosphatidylethanolamine-binding protein; PDBTitle: phosphatidylethanolamine-binding protein from plasmodium vivax 18 c2f91B_ 16.3 46 PDB header:hydrolase/hydrolase inhibitor Chain: B: PDB Molecule:serine protease inhibitor i/ii; PDBTitle: 1.2a resolution structure of a crayfish trypsin complexed2 with a peptide inhibitor, sgti 19 d1uyra2 15.7 29 Fold:ClpP/crotonase Superfamily:ClpP/crotonase Family:Biotin dependent carboxylase carboxyltransferase domain 20 c1wkpA_ 15.2 36 PDB header:signaling protein Chain: A: PDB Molecule:flowering locus t protein; PDBTitle: flowering locus t (ft) from arabidopsis thaliana 21 c3h0jA_ not modelled 13.9 29 PDB header:transferase Chain: A: PDB Molecule:acetyl-coa carboxylase; PDBTitle: crystal structure of the carboxyltransferase domain of2 acetyl-coenzyme a carboxylase in complex with compound 2 22 c3c5pF_ not modelled 13.7 17 PDB header:structural genomics, unknown function Chain: F: PDB Molecule:protein bas0735 of unknown function; PDBTitle: crystal structure of bas0735, a protein of unknown function from2 bacillus anthracis str. sterne 23 c2jyzA_ not modelled 12.6 22 PDB header:unknown function Chain: A: PDB Molecule:cg7054-pa; PDBTitle: cg7054 solution structure 24 c2xu8B_ not modelled 12.1 30 PDB header:structural genomics Chain: B: PDB Molecule:pa1645; PDBTitle: structure of pa1645 25 d1igqa_ not modelled 11.7 28 Fold:SH3-like barrel Superfamily:C-terminal domain of transcriptional repressors Family:Transcriptional repressor protein KorB 26 d1igub_ not modelled 11.6 28 Fold:SH3-like barrel Superfamily:C-terminal domain of transcriptional repressors Family:Transcriptional repressor protein KorB 27 c2dreA_ not modelled 10.4 14 PDB header:plant protein Chain: A: PDB Molecule:water-soluble chlorophyll protein; PDBTitle: crystal structure of water-soluble chlorophyll protein from2 lepidium virginicum at 2.00 angstrom resolution 28 c1uytC_ not modelled 10.3 29 PDB header:transferase Chain: C: PDB Molecule:acetyl-coa carboxylase; PDBTitle: acetyl-coa carboxylase carboxyltransferase domain 29 d1kn3a_ not modelled 10.2 22 Fold:PEBP-like Superfamily:PEBP-like Family:Phosphatidylethanolamine binding protein 30 d1qoua_ not modelled 10.0 36 Fold:PEBP-like Superfamily:PEBP-like Family:Phosphatidylethanolamine binding protein 31 d1khua_ not modelled 9.7 35 Fold:SMAD/FHA domain Superfamily:SMAD/FHA domain Family:SMAD domain 32 c2r77A_ not modelled 9.6 17 PDB header:lipid binding protein Chain: A: PDB Molecule:phosphatidylethanolamine-binding protein, putative; PDBTitle: crystal structure of phosphatidylethanolamine-binding protein,2 pfl0955c, from plasmodium falciparum 33 d1wpxb1 not modelled 9.6 26 Fold:PEBP-like Superfamily:PEBP-like Family:Phosphatidylethanolamine binding protein 34 d1dd1a_ not modelled 9.2 47 Fold:SMAD/FHA domain Superfamily:SMAD/FHA domain Family:SMAD domain 35 d1zxoa2 not modelled 9.1 21 Fold:Ribonuclease H-like motif Superfamily:Actin-like ATPase domain Family:BadF/BadG/BcrA/BcrD-like 36 d1a44a_ not modelled 9.1 22 Fold:PEBP-like Superfamily:PEBP-like Family:Phosphatidylethanolamine binding protein 37 c4a1bB_ not modelled 8.3 38 PDB header:ribosome Chain: B: PDB Molecule:rpl39; PDBTitle: t.thermophila 60s ribosomal subunit in complex with2 initiation factor 6. this file contains 26s rrna and3 proteins of molecule 3. 38 c2l72A_ not modelled 8.0 33 PDB header:protein binding Chain: A: PDB Molecule:actin depolymerizing factor, putative; PDBTitle: solution structure and dynamics of adf from toxoplasma gondii (tgadf) 39 d1o5ua_ not modelled 7.6 21 Fold:Double-stranded beta-helix Superfamily:RmlC-like cupins Family:Hypothetical protein TM1112 40 d1ygsa_ not modelled 7.6 47 Fold:SMAD/FHA domain Superfamily:SMAD/FHA domain Family:SMAD domain 41 d1zbsa1 not modelled 7.5 21 Fold:Ribonuclease H-like motif Superfamily:Actin-like ATPase domain Family:BadF/BadG/BcrA/BcrD-like 42 d1xwva_ not modelled 7.3 16 Fold:Immunoglobulin-like beta-sandwich Superfamily:E set domains Family:ML domain 43 d1u3em2 not modelled 7.1 43 Fold:DNA-binding domain of intron-encoded endonucleases Superfamily:DNA-binding domain of intron-encoded endonucleases Family:DNA-binding domain of intron-encoded endonucleases 44 c3a9lB_ not modelled 6.9 33 PDB header:hydrolase Chain: B: PDB Molecule:poly-gamma-glutamate hydrolase; PDBTitle: structure of bacteriophage poly-gamma-glutamate hydrolase 45 d1khxa_ not modelled 6.9 41 Fold:SMAD/FHA domain Superfamily:SMAD/FHA domain Family:SMAD domain 46 c1khxA_ not modelled 6.9 41 PDB header:transcription Chain: A: PDB Molecule:smad2; PDBTitle: crystal structure of a phosphorylated smad2 47 c2jv4A_ not modelled 6.5 22 PDB header:isomerase Chain: A: PDB Molecule:peptidyl-prolyl cis/trans isomerase; PDBTitle: structure characterisation of pina ww domain and comparison2 with other group iv ww domains, pin1 and ess1 48 d1jjcb3 not modelled 6.3 21 Fold:OB-fold Superfamily:Nucleic acid-binding proteins Family:Myf domain 49 d2jnaa1 not modelled 6.1 46 Fold:Dodecin subunit-like Superfamily:YdgH-like Family:YdgH-like 50 c3q1jA_ not modelled 6.0 21 PDB header:transcription Chain: A: PDB Molecule:phd finger protein 20; PDBTitle: crystal structure of tudor domain 1 of human phd finger protein 20 51 c2zkr3_ not modelled 5.8 83 PDB header:ribosomal protein/rna Chain: 3: PDB Molecule:60s ribosomal protein l39e; PDBTitle: structure of a mammalian ribosomal 60s subunit within an2 80s complex obtained by docking homology models of the rna3 and proteins into an 8.7 a cryo-em map 52 c2x24B_ not modelled 5.3 24 PDB header:ligase Chain: B: PDB Molecule:acetyl-coa carboxylase; PDBTitle: bovine acc2 ct domain in complex with inhibitor

Binding site prediction

Due to computational demand, binding site predictions are not run for batch jobs

If you want to predict binding sites, please manually submit your model of choice to 3DLigandSite

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Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
If you use the binding site predictions from 3DLigandSite, please also cite:
3DLigandSite: predicting ligand-binding sites using similar structures.
Wass MN, Kelley LA and Sternberg MJ Nucleic Acids Research 38, W469-73 (2010) [PubMed]
© Structural Bioinformatics Group Imperial College London Lawrence Kelley, Benjamin Jefferys Disclaimer Terms and Conditions	Component software Template detection: HHpred 1.51 Secondary structure prediction: Psi-pred 2.5 Disorder prediction: Disopred 2.4 Transmembrane prediction: Memsat_SVM Multi-template modelling and ab initio: Poing 1.0