Secondary structure and disorder prediction

		1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	K	H	V	F	K	Y	L	D	F	A	E	D	R	E	H	A	E	S	V	A	T	K	E	L	K	L	D	H	V	E	K	F	A	I	R	D	L	A	N	D	I	K	E	R	G	C	V	E	L	V	Q	P	G	G	F	D	E	L	V
Secondary structure
SS confidence
Disorder		?	?																		?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		Q	I	Y	E	A	G	G	D	G	I	E	P	L	N	C	G	I	E	S	R	K	V	A	I	A	A	L	L	R	V	M	R	E	P	D	F	Q	C	L	E	M	V	H	E	I	I	R	I	A	R	D	L	E	A	P	V	D	A	P	L
Secondary structure
SS confidence
Disorder																																																				?	?				?	?	?	?	?
Disorder confidence
		.	.
Sequence		D	C
Secondary structure
SS confidence
Disorder		?	?
Disorder confidence

Confidence Key
High(9)											Low (0)

?	Disordered
	Alpha helix
	Beta strand

Domain analysis

Hover over an aligned region to see model and summary info

Please note, only up to the top 20 hits are modelled to reduce computer load

Rank Aligned region

PDB 1giq chain A domain 2 Region: 8 - 41 Aligned: 34 Modelled: 34 Confidence: 15.6% Identity: 24% Fold: ADP-ribosylation Superfamily: ADP-ribosylation Family: ADP-ribosylating toxins Phyre2 PDB 1fov chain A Region: 54 - 67 Aligned: 14 Modelled: 14 Confidence: 11.6% Identity: 43% Fold: Thioredoxin fold Superfamily: Thioredoxin-like Family: Thioltransferase Phyre2 PDB 1jhb chain A Region: 54 - 66 Aligned: 13 Modelled: 13 Confidence: 8.5% Identity: 38% Fold: Thioredoxin fold Superfamily: Thioredoxin-like Family: Thioltransferase Phyre2 PDB 3l4n chain A Region: 54 - 66 Aligned: 13 Modelled: 13 Confidence: 8.0% Identity: 31% PDB header:oxidoreductase Chain: A: PDB Molecule:monothiol glutaredoxin-6; PDBTitle: crystal structure of yeast monothiol glutaredoxin grx6 Phyre2 PDB 1qs1 chain A domain 1 Region: 8 - 41 Aligned: 34 Modelled: 34 Confidence: 8.0% Identity: 35% Fold: ADP-ribosylation Superfamily: ADP-ribosylation Family: ADP-ribosylating toxins Phyre2 PDB 3fve chain A Region: 62 - 89 Aligned: 26 Modelled: 28 Confidence: 7.7% Identity: 38% PDB header:isomerase Chain: A: PDB Molecule:diaminopimelate epimerase; PDBTitle: crystal structure of diaminopimelate epimerase mycobacterium2 tuberculosis dapf Phyre2 PDB 1t1v chain A Region: 54 - 67 Aligned: 14 Modelled: 14 Confidence: 7.5% Identity: 21% Fold: Thioredoxin fold Superfamily: Thioredoxin-like Family: SH3BGR (SH3-binding, glutamic acid-rich protein-like) Phyre2 PDB 1ejx chain C domain 2 Region: 57 - 98 Aligned: 40 Modelled: 42 Confidence: 6.8% Identity: 30% Fold: TIM beta/alpha-barrel Superfamily: Metallo-dependent hydrolases Family: alpha-subunit of urease, catalytic domain Phyre2 PDB 2hy5 chain C domain 1 Region: 38 - 64 Aligned: 27 Modelled: 27 Confidence: 6.6% Identity: 22% Fold: DsrEFH-like Superfamily: DsrEFH-like Family: DsrH-like Phyre2 PDB 3hym chain E Region: 24 - 33 Aligned: 10 Modelled: 10 Confidence: 6.6% Identity: 70% PDB header:cell cycle, ligase Chain: E: PDB Molecule:anaphase-promoting complex subunit cdc26; PDBTitle: insights into anaphase promoting complex tpr subdomain2 assembly from a cdc26-apc6 structure Phyre2 PDB 3ogm chain B Region: 3 - 20 Aligned: 18 Modelled: 18 Confidence: 6.5% Identity: 33% PDB header:protein binding Chain: B: PDB Molecule:coronatine-insensitive protein 1; PDBTitle: structure of coi1-ask1 in complex with coronatine and the jaz1 degron Phyre2 PDB 1kte chain A Region: 54 - 66 Aligned: 13 Modelled: 13 Confidence: 6.4% Identity: 31% Fold: Thioredoxin fold Superfamily: Thioredoxin-like Family: Thioltransferase Phyre2 PDB 3ogl chain D Region: 3 - 20 Aligned: 18 Modelled: 18 Confidence: 6.3% Identity: 33% PDB header:protein binding Chain: D: PDB Molecule:coronatine-insensitive protein 1; PDBTitle: structure of coi1-ask1 in complex with ja-isoleucine and the jaz12 degron Phyre2 PDB 3hym chain K Region: 24 - 33 Aligned: 10 Modelled: 10 Confidence: 6.2% Identity: 70% PDB header:cell cycle, ligase Chain: K: PDB Molecule:anaphase-promoting complex subunit cdc26; PDBTitle: insights into anaphase promoting complex tpr subdomain2 assembly from a cdc26-apc6 structure Phyre2 PDB 3ljb chain A Region: 72 - 112 Aligned: 41 Modelled: 41 Confidence: 6.2% Identity: 12% PDB header:antiviral protein Chain: A: PDB Molecule:interferon-induced gtp-binding protein mx1; PDBTitle: structural basis of oligomerisation in the mxa stalk Phyre2 PDB 1qb5 chain D Region: 78 - 92 Aligned: 15 Modelled: 15 Confidence: 6.1% Identity: 60% Fold: OB-fold Superfamily: Bacterial enterotoxins Family: Bacterial AB5 toxins, B-subunits Phyre2 PDB 2wul chain B Region: 54 - 66 Aligned: 13 Modelled: 13 Confidence: 6.1% Identity: 46% PDB header:oxidoreductase Chain: B: PDB Molecule:glutaredoxin related protein 5; PDBTitle: crystal structure of the human glutaredoxin 5 with bound2 glutathione in an fes cluster Phyre2 PDB 2gke chain A domain 2 Region: 63 - 89 Aligned: 25 Modelled: 27 Confidence: 6.0% Identity: 28% Fold: Diaminopimelate epimerase-like Superfamily: Diaminopimelate epimerase-like Family: Diaminopimelate epimerase Phyre2 PDB 2hzf chain A Region: 54 - 66 Aligned: 13 Modelled: 13 Confidence: 5.9% Identity: 31% PDB header:electron transport, oxidoreductase Chain: A: PDB Molecule:glutaredoxin-1; PDBTitle: crystal structures of a poxviral glutaredoxin in the oxidized and2 reduced states show redox-correlated structural changes Phyre2 PDB 1kny chain A domain 1 Region: 53 - 66 Aligned: 14 Modelled: 14 Confidence: 5.9% Identity: 43% Fold: Four-helical up-and-down bundle Superfamily: Nucleotidyltransferase substrate binding subunit/domain Family: Kanamycin nucleotidyltransferase (KNTase), C-terminal domain Phyre2 1 d1giqa2 2 d1fova_ 3 d1jhba_ 4 c3l4nA_ 5 d1qs1a1 6 c3fveA_ 7 d1t1va_ 8 d1ejxc2 9 d2hy5c1 10 c3hymE_ 11 c3ogmB_ 12 d1ktea_ 13 c3oglD_ 14 c3hymK_ 15 c3ljbA_ 16 d1qb5d_ 17 c2wulB_ 18 d2gkea2 19 c2hzfA_ 20 d1knya1 21 22 23 24 25 26 27 28 29 30 31

Detailed template information

# Template Alignment Coverage 3D Model Confidence % i.d. Template Information

1 d1giqa2 15.6 24 Fold:ADP-ribosylation Superfamily:ADP-ribosylation Family:ADP-ribosylating toxins 2 d1fova_ 11.6 43 Fold:Thioredoxin fold Superfamily:Thioredoxin-like Family:Thioltransferase 3 d1jhba_ 8.5 38 Fold:Thioredoxin fold Superfamily:Thioredoxin-like Family:Thioltransferase 4 c3l4nA_ 8.0 31 PDB header:oxidoreductase Chain: A: PDB Molecule:monothiol glutaredoxin-6; PDBTitle: crystal structure of yeast monothiol glutaredoxin grx6 5 d1qs1a1 8.0 35 Fold:ADP-ribosylation Superfamily:ADP-ribosylation Family:ADP-ribosylating toxins 6 c3fveA_ 7.7 38 PDB header:isomerase Chain: A: PDB Molecule:diaminopimelate epimerase; PDBTitle: crystal structure of diaminopimelate epimerase mycobacterium2 tuberculosis dapf 7 d1t1va_ 7.5 21 Fold:Thioredoxin fold Superfamily:Thioredoxin-like Family:SH3BGR (SH3-binding, glutamic acid-rich protein-like) 8 d1ejxc2 6.8 30 Fold:TIM beta/alpha-barrel Superfamily:Metallo-dependent hydrolases Family:alpha-subunit of urease, catalytic domain 9 d2hy5c1 6.6 22 Fold:DsrEFH-like Superfamily:DsrEFH-like Family:DsrH-like 10 c3hymE_ 6.6 70 PDB header:cell cycle, ligase Chain: E: PDB Molecule:anaphase-promoting complex subunit cdc26; PDBTitle: insights into anaphase promoting complex tpr subdomain2 assembly from a cdc26-apc6 structure 11 c3ogmB_ 6.5 33 PDB header:protein binding Chain: B: PDB Molecule:coronatine-insensitive protein 1; PDBTitle: structure of coi1-ask1 in complex with coronatine and the jaz1 degron 12 d1ktea_ 6.4 31 Fold:Thioredoxin fold Superfamily:Thioredoxin-like Family:Thioltransferase 13 c3oglD_ 6.3 33 PDB header:protein binding Chain: D: PDB Molecule:coronatine-insensitive protein 1; PDBTitle: structure of coi1-ask1 in complex with ja-isoleucine and the jaz12 degron 14 c3hymK_ 6.2 70 PDB header:cell cycle, ligase Chain: K: PDB Molecule:anaphase-promoting complex subunit cdc26; PDBTitle: insights into anaphase promoting complex tpr subdomain2 assembly from a cdc26-apc6 structure 15 c3ljbA_ 6.2 12 PDB header:antiviral protein Chain: A: PDB Molecule:interferon-induced gtp-binding protein mx1; PDBTitle: structural basis of oligomerisation in the mxa stalk 16 d1qb5d_ 6.1 60 Fold:OB-fold Superfamily:Bacterial enterotoxins Family:Bacterial AB5 toxins, B-subunits 17 c2wulB_ 6.1 46 PDB header:oxidoreductase Chain: B: PDB Molecule:glutaredoxin related protein 5; PDBTitle: crystal structure of the human glutaredoxin 5 with bound2 glutathione in an fes cluster 18 d2gkea2 6.0 28 Fold:Diaminopimelate epimerase-like Superfamily:Diaminopimelate epimerase-like Family:Diaminopimelate epimerase 19 c2hzfA_ 5.9 31 PDB header:electron transport, oxidoreductase Chain: A: PDB Molecule:glutaredoxin-1; PDBTitle: crystal structures of a poxviral glutaredoxin in the oxidized and2 reduced states show redox-correlated structural changes 20 d1knya1 5.9 43 Fold:Four-helical up-and-down bundle Superfamily:Nucleotidyltransferase substrate binding subunit/domain Family:Kanamycin nucleotidyltransferase (KNTase), C-terminal domain 21 c3hymA_ not modelled 5.9 70 PDB header:cell cycle, ligase Chain: A: PDB Molecule:anaphase-promoting complex subunit cdc26; PDBTitle: insights into anaphase promoting complex tpr subdomain2 assembly from a cdc26-apc6 structure 22 c3hymI_ not modelled 5.9 70 PDB header:cell cycle, ligase Chain: I: PDB Molecule:anaphase-promoting complex subunit cdc26; PDBTitle: insights into anaphase promoting complex tpr subdomain2 assembly from a cdc26-apc6 structure 23 c2ht9A_ not modelled 5.7 21 PDB header:oxidoreductase Chain: A: PDB Molecule:glutaredoxin-2; PDBTitle: the structure of dimeric human glutaredoxin 2 24 d2f6mb1 not modelled 5.4 29 Fold:Long alpha-hairpin Superfamily:Endosomal sorting complex assembly domain Family:VPS28 N-terminal domain 25 d1p3qq_ not modelled 5.3 18 Fold:RuvA C-terminal domain-like Superfamily:UBA-like Family:CUE domain 26 c3hymC_ not modelled 5.3 70 PDB header:cell cycle, ligase Chain: C: PDB Molecule:anaphase-promoting complex subunit cdc26; PDBTitle: insights into anaphase promoting complex tpr subdomain2 assembly from a cdc26-apc6 structure 27 d1r45a_ not modelled 5.3 29 Fold:ADP-ribosylation Superfamily:ADP-ribosylation Family:ADP-ribosylating toxins 28 c2e7pC_ not modelled 5.2 31 PDB header:electron transport Chain: C: PDB Molecule:glutaredoxin; PDBTitle: crystal structure of the holo form of glutaredoxin c1 from populus2 tremula x tremuloides 29 c2cazB_ not modelled 5.1 29 PDB header:protein transport Chain: B: PDB Molecule:vacuolar protein sorting-associated protein PDBTitle: escrt-i core 30 d2cazb1 not modelled 5.1 29 Fold:Long alpha-hairpin Superfamily:Endosomal sorting complex assembly domain Family:VPS28 N-terminal domain 31 c3hymG_ not modelled 5.1 70 PDB header:cell cycle, ligase Chain: G: PDB Molecule:anaphase-promoting complex subunit cdc26; PDBTitle: insights into anaphase promoting complex tpr subdomain2 assembly from a cdc26-apc6 structure

Binding site prediction

Due to computational demand, binding site predictions are not run for batch jobs

If you want to predict binding sites, please manually submit your model of choice to 3DLigandSite

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Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
If you use the binding site predictions from 3DLigandSite, please also cite:
3DLigandSite: predicting ligand-binding sites using similar structures.
Wass MN, Kelley LA and Sternberg MJ Nucleic Acids Research 38, W469-73 (2010) [PubMed]
© Structural Bioinformatics Group Imperial College London Lawrence Kelley, Benjamin Jefferys Disclaimer Terms and Conditions	Component software Template detection: HHpred 1.51 Secondary structure prediction: Psi-pred 2.5 Disorder prediction: Disopred 2.4 Transmembrane prediction: Memsat_SVM Multi-template modelling and ab initio: Poing 1.0