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Job DescriptionP32138
Confidence99.96%DateThu Jan 5 11:49:24 GMT 2012
Rank14Aligned Residues592
% Identity12%Templatec2xn1B_
PDB info PDB header:hydrolaseChain: B: PDB Molecule:alpha-galactosidase; PDBTitle: structure of alpha-galactosidase from lactobacillus acidophilus ncfm2 with tris
Resolution2.30 Å

  Insertion relative to template
  Deletion relative to template
  Catalytic residue from the CSA
 
Detailed help on interpreting your alignment


   36...40... ......50.........60.........70.........80..... ....90.........100......
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Query Sequence  NPCLWIGS. . GIADIDMFRGNFSIKDKLQEKIALTDAIVSQSPDGWLIHFSR. . . GSDISATLNISADDQGRLLLE. . . .
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Template Sequence  TPATIVRNPDGSNALFLAYKSYKIEDGKPDLKGLPHSWTKEDDEAQTLIVTLEDKVSKLEYDLLYTIYRDRPVIVRSVQV
Template Known Secondary structure 

S
TTS





S



TTS




SSGGGTTTTTTSS
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   90.........100.........110.........120.........130.........140.........150.........160.........
 
   107..110........ .120....... ..130.........140.........150.........160.........170.........
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Query Sequence  . . LQNDNLNHNRIW. LRLAAQPED. . . . HIYGCGEQFSYFDLRGKPFPLWTSEQGVGRNKQTYVTWQADCKENAGGDYYW
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Template Conservation   
 
   
 
    
  
             
 
  
       
  
     
  
 

    
   
         
 
Template Sequence  HNHGEEAVYLEKVASMQMDYVDKDFEVITLPGAHANERRVQRENIGQGIKVFSSYRGTSSHQMNPFMALVDHDTNEFMGE
Template Known Secondary structure 
SSS
SS



BTTB





S

SSSS
SS
TT

SSSB
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   170.........180.........190.........200.........210.........220.........230.........240.........
 
   180.........190.........200........ .210.........220.. .......230.........240.........250...
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Query Sequence  TFFPQPTFVSTQKYYCHVDNSCYMNFDFS. . APEYHELALWEDKA. . . . TLRFECADTYISLLEKLTALLGRQPELPDWI
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Template Sequence  AYGFALAYSGNHKFEVERDQFGQIHVNTGINDYNFKWKLNPNEEFQTPEVLMVYSDQGLNKMSQAFHSLIHERIMRSKFK
Template Known Secondary structure 

SS

TTS


TT


TT



SB
T


TTT
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   250.........260.........270.........280.........290.........300.........310.........320.........
 
   254.....260... ......270.........280.........290.........300.........310..... ....320....
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Query Sequence  YDGVTLGIQG. . . . . . . . GTEVCQKKLDTMRNAGVKVNGIWAQDWSGIRMTSFGKRVMWNWKWNSENYPQ. LDSRIKQWN
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Template Sequence  DQIRPVLVNNWEATYFDFNEDKLKTIVDKAKKLGLEM. . FVLDDGWF. GHRDDDNSSLGDWKVYKKKFPNGLGHFADYVH
Template Known Secondary structure  TS




TT


T

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TTSB.TT
SSTTS
TT

SB
TTT
TT
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   330.........340.........350.........360...... ...370.... .....380.........390.........400......
 
   325....330...... ...340.........350.........360.........370.........380.........390.........400...
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Query Sequence  QEGVQFLAYINP. YVASDKDLCEEAAQHGYLAKDASGGDYLVEFGEFYGGVVDLTNPEAYAWFKEVIKKNMIELGCGGWM
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Template Sequence  EQGLKFGLWFEPEMISYESNLYKEHP. . DYLMHVPGRKP. . . . CPSRNQYVLELGRKEVRDNIFEQMVKILDSKKIDYIK
Template Known Secondary structure  TT

TT
SSS
G..GGB


TTS

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BTTBB
TTSTTS


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   407..410.........420.........430.. .......440... ......450.........460.........470.........480
 
   404.....410 .........420.........430.........440.........450.........460.. .......470......
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Query Sequence  ADFGEYL. . PTDTYLHNGVSAEIMHNAWPALWAKCNYEALEETGKLGEILFFMRAGSTGSQ. . . . . KYSTMMWAGDQNVD
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Template Sequence  WDMNRSLSDIYESDLPADQQGEAYHRYVLGYYD. . LLNKLVTRYPDI. . LFEGCSGGGGRFDVGQAYYTPQIWASDNTD.
Template Known Secondary structure 


S


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TTS
GGGGGG..
TTS..
BTBBTT
STT
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BS


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   481........490.........500.........510... ......520..... ....530.........540.........550.....
 
   477..480.........490.........500.........510.........520.........530.........540.........550......
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Query Sequence  WSLDDGLASVVPAALSLAMTGHGLHHSDIGGYTTLFEMKRSKELLLRWCDFSAFTPMMRTHEGNRPGDNWQFDGDAETIA
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Template Sequence  . . . . . . AIERLKIQYGTSLVYPQSMMTSHVSVSPNEQNGRITPFNTRG. . AVAMWGDLGYE. . . . . LDLTKM. . SDEESD
Template Known Secondary structure  ......TTTS
GGG
SS
TTT




..TTS.....S
GGG
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   556...560.........570.........580.........590....... ..600........ .610.... .....620
 
   557..560.........570.........580.........590.........600.........610.........620.........630 .....
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Query Sequence  HFARMTTVFTTLKPYLKEAVALNAKSGLPVMRPLFLHYEDDAHTYTLKYQYLLGRDILVAPVHEEGRSDWTLYL. PEDNW
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Template Sequence  QVVKQVTEYKKIREVTQFG. . TLYRLKASASNQCAWMMVDSN. . . . . KNEAVVTVVNVMAHAQPYCTKTKLAGLDPDKRY
Template Known Secondary structure  S..
GGGT
TT.....S

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SS






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   621........630......... 640.........650.........660 .........670.........680.........690...
 
   636...640........ .650.........660...
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Query Sequence  VHAWTGEAFRGGE. . . VTVNAPIGKPPVFYR
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Template Conservation   
       

  

  

 

    
  
 
Template Sequence  KNLETDEVFGGDELMHLGFYDPIERGDFKAK
Template Known Secondary structure  TTT

T





SSS
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   694.....700.........710.........720....
 
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Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
 
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Lawrence Kelley, Benjamin Jefferys 
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