Secondary structure and disorder prediction

		1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	P	L	P	D	F	H	V	S	E	P	F	T	L	G	I	E	L	E	M	Q	V	V	N	P	P	G	Y	D	L	S	Q	D	S	S	M	L	I	D	A	V	K	N	K	I	T	A	G	E	V	K	H	D	I	T	E	S	M	L	E
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?	?	?	?																		?		?	?	?	?											?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		L	A	T	D	V	C	R	D	I	N	Q	A	A	G	Q	F	S	A	M	Q	K	V	V	L	Q	A	A	T	D	H	H	L	E	I	C	G	G	G	T	H	P	F	Q	K	W	Q	R	Q	E	V	C	D	N	E	R	Y	Q	R	T	L
Secondary structure
SS confidence
Disorder																																																?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		E	N	F	G	Y	L	I	Q	Q	A	T	V	F	G	Q	H	V	H	V	G	C	A	S	G	D	D	A	I	Y	L	L	H	G	L	S	R	F	V	P	H	F	I	A	L	S	A	A	S	P	Y	M	Q	G	T	D	T	R	F	A	S
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		S	R	P	N	I	F	S	A	F	P	D	N	G	P	M	P	W	V	S	N	W	Q	Q	F	E	A	L	F	R	C	L	S	Y	T	T	M	I	D	S	I	K	D	L	H	W	D	I	R	P	S	P	H	F	G	T	V	E	V	R	V
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300
Sequence		M	D	T	P	L	T	L	S	H	A	V	N	M	A	G	L	I	Q	A	T	A	H	W	L	L	T	E	R	P	F	K	H	Q	E	K	D	Y	L	L	Y	K	F	N	R	F	Q	A	C	R	Y	G	L	E	G	V	I	T	D	P	H
Secondary structure
SS confidence
Disorder				?																												?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	310	.	.	.	.	.	.	.	.	.	320	.	.	.	.	.	.	.	.	.	330	.	.	.	.	.	.	.	.	.	340	.	.	.	.	.	.	.	.	.	350	.	.	.	.	.	.	.	.	.	360
Sequence		T	G	D	R	R	P	L	T	E	D	T	L	R	L	L	E	K	I	A	P	S	A	H	K	I	G	A	S	S	A	I	E	A	L	H	R	Q	V	V	S	G	L	N	E	A	Q	L	M	R	D	F	V	A	D	G	G	S	L	I	G
Secondary structure
SS confidence
Disorder																																												?											?
Disorder confidence
		.	.	.	.	.	.	.	.	.	370	.	.
Sequence		L	V	K	K	H	C	E	I	W	A	G	D
Secondary structure
SS confidence
Disorder											?	?	?
Disorder confidence

Confidence Key
High(9)											Low (0)

?	Disordered
	Alpha helix
	Beta strand

Domain analysis

Hover over an aligned region to see model and summary info

Please note, only up to the top 20 hits are modelled to reduce computer load

Rank Aligned region

PDB 1r8g chain A Region: 3 - 370 Aligned: 352 Modelled: 352 Confidence: 100.0% Identity: 97% Fold: Glutamine synthetase/guanido kinase Superfamily: Glutamine synthetase/guanido kinase Family: Glutamate-cysteine ligase family 2 (GCS2) Phyre2 PDB 1tt4 chain A Region: 5 - 370 Aligned: 354 Modelled: 365 Confidence: 100.0% Identity: 84% Fold: Glutamine synthetase/guanido kinase Superfamily: Glutamine synthetase/guanido kinase Family: Glutamate-cysteine ligase family 2 (GCS2) Phyre2 PDB 1tt4 chain B Region: 5 - 370 Aligned: 354 Modelled: 366 Confidence: 100.0% Identity: 84% PDB header:structural genomics, unknown function Chain: B: PDB Molecule:putative cytoplasmic protein; PDBTitle: structure of np459575, a predicted glutathione synthase from2 salmonella typhimurium Phyre2 PDB 2gwc chain E Region: 7 - 366 Aligned: 352 Modelled: 345 Confidence: 100.0% Identity: 15% PDB header:ligase Chain: E: PDB Molecule:glutamate cysteine ligase; PDBTitle: crystal structure of plant glutamate cysteine ligase in complex with a2 transition state analogue Phyre2 PDB 3nzt chain A Region: 5 - 370 Aligned: 358 Modelled: 366 Confidence: 100.0% Identity: 18% PDB header:ligase Chain: A: PDB Molecule:glutamate--cysteine ligase; PDBTitle: 2.0 angstrom crystal structure of glutamate--cysteine ligase (gsha)2 ftom francisella tularensis in complex with amp Phyre2 PDB 2d32 chain A domain 1 Region: 11 - 370 Aligned: 352 Modelled: 360 Confidence: 100.0% Identity: 17% Fold: Glutamine synthetase/guanido kinase Superfamily: Glutamine synthetase/guanido kinase Family: Glutamate-cysteine ligase Phyre2 PDB 3ln7 chain A Region: 13 - 370 Aligned: 339 Modelled: 358 Confidence: 100.0% Identity: 17% PDB header:ligase Chain: A: PDB Molecule:glutathione biosynthesis bifunctional protein gshab; PDBTitle: crystal structure of a bifunctional glutathione synthetase from2 pasteurella multocida Phyre2 PDB 3ln6 chain A Region: 13 - 370 Aligned: 339 Modelled: 349 Confidence: 100.0% Identity: 16% PDB header:ligase Chain: A: PDB Molecule:glutathione biosynthesis bifunctional protein gshab; PDBTitle: crystal structure of a bifunctional glutathione synthetase from2 streptococcus agalactiae Phyre2 PDB 3lvw chain A Region: 7 - 287 Aligned: 278 Modelled: 276 Confidence: 99.8% Identity: 21% PDB header:ligase Chain: A: PDB Molecule:glutamate--cysteine ligase; PDBTitle: glutathione-inhibited scgcl Phyre2 PDB 1f52 chain A domain 2 Region: 12 - 273 Aligned: 201 Modelled: 208 Confidence: 99.2% Identity: 18% Fold: Glutamine synthetase/guanido kinase Superfamily: Glutamine synthetase/guanido kinase Family: Glutamine synthetase catalytic domain Phyre2 PDB 2bvc chain A domain 2 Region: 13 - 273 Aligned: 196 Modelled: 203 Confidence: 99.1% Identity: 18% Fold: Glutamine synthetase/guanido kinase Superfamily: Glutamine synthetase/guanido kinase Family: Glutamine synthetase catalytic domain Phyre2 PDB 1fpy chain E Region: 9 - 273 Aligned: 200 Modelled: 203 Confidence: 99.1% Identity: 19% PDB header:ligase Chain: E: PDB Molecule:glutamine synthetase; PDBTitle: crystal structure of glutamine synthetase from salmonella2 typhimurium with inhibitor phosphinothricin Phyre2 PDB 3ng0 chain A Region: 9 - 273 Aligned: 200 Modelled: 207 Confidence: 99.1% Identity: 18% PDB header:ligase Chain: A: PDB Molecule:glutamine synthetase; PDBTitle: crystal structure of glutamine synthetase from synechocystis sp. pcc2 6803 Phyre2 PDB 1hto chain B Region: 9 - 273 Aligned: 200 Modelled: 207 Confidence: 99.0% Identity: 19% PDB header:ligase Chain: B: PDB Molecule:glutamine synthetase; PDBTitle: crystallographic structure of a relaxed glutamine synthetase from2 mycobacterium tuberculosis Phyre2 PDB 2qc8 chain J Region: 12 - 261 Aligned: 196 Modelled: 215 Confidence: 98.8% Identity: 14% PDB header:ligase Chain: J: PDB Molecule:glutamine synthetase; PDBTitle: crystal structure of human glutamine synthetase in complex with adp2 and methionine sulfoximine phosphate Phyre2 PDB 3o6x chain C Region: 12 - 260 Aligned: 193 Modelled: 209 Confidence: 98.7% Identity: 20% PDB header:ligase Chain: C: PDB Molecule:glutamine synthetase; PDBTitle: crystal structure of the type iii glutamine synthetase from2 bacteroides fragilis Phyre2 PDB 2d3a chain J Region: 12 - 263 Aligned: 190 Modelled: 195 Confidence: 98.6% Identity: 15% PDB header:ligase Chain: J: PDB Molecule:glutamine synthetase; PDBTitle: crystal structure of the maize glutamine synthetase2 complexed with adp and methionine sulfoximine phosphate Phyre2 PDB 3fky chain D Region: 12 - 261 Aligned: 183 Modelled: 193 Confidence: 98.5% Identity: 21% PDB header:ligase Chain: D: PDB Molecule:glutamine synthetase; PDBTitle: crystal structure of the glutamine synthetase gln1deltan182 from the yeast saccharomyces cerevisiae Phyre2 PDB 2j9i chain L Region: 7 - 273 Aligned: 212 Modelled: 227 Confidence: 98.4% Identity: 13% PDB header:ligase Chain: L: PDB Molecule:glutamate-ammonia ligase domain-containing PDBTitle: lengsin is a survivor of an ancient family of class i2 glutamine synthetases in eukaryotes that has undergone3 evolutionary re-engineering for a tissue-specific role4 in the vertebrate eye lens. Phyre2 PDB 3oqh chain B Region: 72 - 181 Aligned: 103 Modelled: 110 Confidence: 44.1% Identity: 14% PDB header:ligase Chain: B: PDB Molecule:putative uncharacterized protein yvmc; PDBTitle: crystal structure of b. licheniformis cdps yvmc-blic Phyre2 1 d1r8ga_ 2 d1tt4a_ 3 c1tt4B_ 4 c2gwcE_ 5 c3nztA_ 6 d2d32a1 7 c3ln7A_ 8 c3ln6A_ 9 c3lvwA_ 10 d1f52a2 11 d2bvca2 12 c1fpyE_ 13 c3ng0A_ 14 c1htoB_ 15 c2qc8J_ 16 c3o6xC_ 17 c2d3aJ_ 18 c3fkyD_ 19 c2j9iL_ 20 c3oqhB_ 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59

Detailed template information

# Template Alignment Coverage 3D Model Confidence % i.d. Template Information

1 d1r8ga_ 100.0 97 Fold:Glutamine synthetase/guanido kinase Superfamily:Glutamine synthetase/guanido kinase Family:Glutamate-cysteine ligase family 2 (GCS2) 2 d1tt4a_ 100.0 84 Fold:Glutamine synthetase/guanido kinase Superfamily:Glutamine synthetase/guanido kinase Family:Glutamate-cysteine ligase family 2 (GCS2) 3 c1tt4B_ 100.0 84 PDB header:structural genomics, unknown function Chain: B: PDB Molecule:putative cytoplasmic protein; PDBTitle: structure of np459575, a predicted glutathione synthase from2 salmonella typhimurium 4 c2gwcE_ 100.0 15 PDB header:ligase Chain: E: PDB Molecule:glutamate cysteine ligase; PDBTitle: crystal structure of plant glutamate cysteine ligase in complex with a2 transition state analogue 5 c3nztA_ 100.0 18 PDB header:ligase Chain: A: PDB Molecule:glutamate--cysteine ligase; PDBTitle: 2.0 angstrom crystal structure of glutamate--cysteine ligase (gsha)2 ftom francisella tularensis in complex with amp 6 d2d32a1 100.0 17 Fold:Glutamine synthetase/guanido kinase Superfamily:Glutamine synthetase/guanido kinase Family:Glutamate-cysteine ligase 7 c3ln7A_ 100.0 17 PDB header:ligase Chain: A: PDB Molecule:glutathione biosynthesis bifunctional protein gshab; PDBTitle: crystal structure of a bifunctional glutathione synthetase from2 pasteurella multocida 8 c3ln6A_ 100.0 16 PDB header:ligase Chain: A: PDB Molecule:glutathione biosynthesis bifunctional protein gshab; PDBTitle: crystal structure of a bifunctional glutathione synthetase from2 streptococcus agalactiae 9 c3lvwA_ 99.8 21 PDB header:ligase Chain: A: PDB Molecule:glutamate--cysteine ligase; PDBTitle: glutathione-inhibited scgcl 10 d1f52a2 99.2 18 Fold:Glutamine synthetase/guanido kinase Superfamily:Glutamine synthetase/guanido kinase Family:Glutamine synthetase catalytic domain 11 d2bvca2 99.1 18 Fold:Glutamine synthetase/guanido kinase Superfamily:Glutamine synthetase/guanido kinase Family:Glutamine synthetase catalytic domain 12 c1fpyE_ 99.1 19 PDB header:ligase Chain: E: PDB Molecule:glutamine synthetase; PDBTitle: crystal structure of glutamine synthetase from salmonella2 typhimurium with inhibitor phosphinothricin 13 c3ng0A_ 99.1 18 PDB header:ligase Chain: A: PDB Molecule:glutamine synthetase; PDBTitle: crystal structure of glutamine synthetase from synechocystis sp. pcc2 6803 14 c1htoB_ 99.0 19 PDB header:ligase Chain: B: PDB Molecule:glutamine synthetase; PDBTitle: crystallographic structure of a relaxed glutamine synthetase from2 mycobacterium tuberculosis 15 c2qc8J_ 98.8 14 PDB header:ligase Chain: J: PDB Molecule:glutamine synthetase; PDBTitle: crystal structure of human glutamine synthetase in complex with adp2 and methionine sulfoximine phosphate 16 c3o6xC_ 98.7 20 PDB header:ligase Chain: C: PDB Molecule:glutamine synthetase; PDBTitle: crystal structure of the type iii glutamine synthetase from2 bacteroides fragilis 17 c2d3aJ_ 98.6 15 PDB header:ligase Chain: J: PDB Molecule:glutamine synthetase; PDBTitle: crystal structure of the maize glutamine synthetase2 complexed with adp and methionine sulfoximine phosphate 18 c3fkyD_ 98.5 21 PDB header:ligase Chain: D: PDB Molecule:glutamine synthetase; PDBTitle: crystal structure of the glutamine synthetase gln1deltan182 from the yeast saccharomyces cerevisiae 19 c2j9iL_ 98.4 13 PDB header:ligase Chain: L: PDB Molecule:glutamate-ammonia ligase domain-containing PDBTitle: lengsin is a survivor of an ancient family of class i2 glutamine synthetases in eukaryotes that has undergone3 evolutionary re-engineering for a tissue-specific role4 in the vertebrate eye lens. 20 c3oqhB_ 44.1 14 PDB header:ligase Chain: B: PDB Molecule:putative uncharacterized protein yvmc; PDBTitle: crystal structure of b. licheniformis cdps yvmc-blic 21 d2qkwa1 not modelled 13.1 11 Fold:immunoglobulin/albumin-binding domain-like Superfamily:Avirulence protein AvrPto Family:Avirulence protein AvrPto 22 c2qkwA_ not modelled 13.1 11 PDB header:transferase Chain: A: PDB Molecule:avirulence protein; PDBTitle: structural basis for activation of plant immunity by2 bacterial effector protein avrpto 23 d1dpsa_ not modelled 12.1 5 Fold:Ferritin-like Superfamily:Ferritin-like Family:Ferritin 24 c2chpC_ not modelled 11.1 7 PDB header:dna-binding protein Chain: C: PDB Molecule:metalloregulation dna-binding stress protein; PDBTitle: crystal structure of the dodecameric ferritin mrga from2 b.subtilis 168 25 c3oqvA_ not modelled 10.9 13 PDB header:protein binding Chain: A: PDB Molecule:albc; PDBTitle: albc, a cyclodipeptide synthase from streptomyces noursei 26 d1p94a_ not modelled 10.8 19 Fold:Ribbon-helix-helix Superfamily:Ribbon-helix-helix Family:CopG-like 27 c2xgwA_ not modelled 10.5 5 PDB header:metal binding protein Chain: A: PDB Molecule:peroxide resistance protein; PDBTitle: zinc-bound crystal structure of streptococcus pyogenes dpr 28 d2eg6a1 not modelled 10.4 21 Fold:TIM beta/alpha-barrel Superfamily:Metallo-dependent hydrolases Family:Dihydroorotase 29 c2c6rA_ not modelled 10.2 7 PDB header:dna-binding protein Chain: A: PDB Molecule:dna-binding stress response protein, dps family; PDBTitle: fe-soaked crystal structure of the dps92 from deinococcus2 radiodurans 30 c2vxxB_ not modelled 9.0 15 PDB header:dna-binding protein Chain: B: PDB Molecule:starvation induced dna binding protein; PDBTitle: x-ray structure of dpsa from thermosynechococcus elongatus 31 c1g5jB_ not modelled 9.0 55 PDB header:apoptosis Chain: B: PDB Molecule:bad protein; PDBTitle: complex of bcl-xl with peptide from bad 32 c3iq1A_ not modelled 8.4 7 PDB header:metal transport Chain: A: PDB Molecule:dps family protein; PDBTitle: crystal structure of dps protein from vibrio cholerae o1, a member of2 a broad superfamily of ferritin-like diiron-carboxylate proteins 33 d1tjoa_ not modelled 8.2 7 Fold:Ferritin-like Superfamily:Ferritin-like Family:Ferritin 34 d1ji4a_ not modelled 8.2 15 Fold:Ferritin-like Superfamily:Ferritin-like Family:Ferritin 35 d2d6fc3 not modelled 7.5 19 Fold:Glutamine synthetase/guanido kinase Superfamily:Glutamine synthetase/guanido kinase Family:GatB/GatE catalytic domain-like 36 c2yjkF_ not modelled 7.4 7 PDB header:metal-binding protein Chain: F: PDB Molecule:afp; PDBTitle: structure of dps from microbacterium arborescens in the2 high iron form 37 c2krkA_ not modelled 7.2 16 PDB header:protein binding Chain: A: PDB Molecule:26s protease regulatory subunit 8; PDBTitle: solution nmr structure of 26s protease regulatory subunit 82 from h.sapiens, northeast structural genomics consortium3 target target hr3102a 38 c2wlaA_ not modelled 6.9 5 PDB header:oxidoreductase Chain: A: PDB Molecule:dps-like peroxide resistance protein; PDBTitle: streptococcus pyogenes dpr 39 c2f7nA_ not modelled 6.9 7 PDB header:dna binding protein Chain: A: PDB Molecule:dna-binding stress response protein, dps family; PDBTitle: structure of d. radiodurans dps-1 40 d2yw6a1 not modelled 6.6 12 Fold:Ferritin-like Superfamily:Ferritin-like Family:Ferritin 41 c2c41K_ not modelled 6.5 7 PDB header:iron-binding/oxidation protein Chain: K: PDB Molecule:dps family dna-binding stress response protein; PDBTitle: x-ray structure of dps from thermosynechococcus elongatus 42 c3rj1G_ not modelled 6.5 38 PDB header:transcription Chain: G: PDB Molecule:mediator of rna polymerase ii transcription subunit 6; PDBTitle: architecture of the mediator head module 43 d1n1qa_ not modelled 6.3 7 Fold:Ferritin-like Superfamily:Ferritin-like Family:Ferritin 44 c2d5kC_ not modelled 6.3 12 PDB header:metal binding protein Chain: C: PDB Molecule:dps family protein; PDBTitle: crystal structure of dps from staphylococcus aureus 45 d1o9ra_ not modelled 6.2 5 Fold:Ferritin-like Superfamily:Ferritin-like Family:Ferritin 46 c2bzwB_ not modelled 6.0 56 PDB header:transcription Chain: B: PDB Molecule:bcl2-antagonist of cell death; PDBTitle: the crystal structure of bcl-xl in complex with full-length2 bad 47 c2z90D_ not modelled 5.9 5 PDB header:dna binding protein Chain: D: PDB Molecule:starvation-inducible dna-binding protein or fine PDBTitle: crystal structure of the second dps from mycobacterium2 smegmatis 48 d1umna_ not modelled 5.9 10 Fold:Ferritin-like Superfamily:Ferritin-like Family:Ferritin 49 d1jiga_ not modelled 5.9 7 Fold:Ferritin-like Superfamily:Ferritin-like Family:Ferritin 50 d2fjca1 not modelled 5.9 10 Fold:Ferritin-like Superfamily:Ferritin-like Family:Ferritin 51 c3hfhB_ not modelled 5.9 23 PDB header:transcription regulator Chain: B: PDB Molecule:transcription elongation regulator 1; PDBTitle: crystal structure of tandem ff domains 52 d1ji5a_ not modelled 5.8 10 Fold:Ferritin-like Superfamily:Ferritin-like Family:Ferritin 53 c2q25C_ not modelled 5.7 12 PDB header:virus,hydrolase/rna Chain: C: PDB Molecule:protein alpha; PDBTitle: flock house virus coat protein d75n mutant 54 d2bk6a1 not modelled 5.7 7 Fold:Ferritin-like Superfamily:Ferritin-like Family:Ferritin 55 c3kwoA_ not modelled 5.7 2 PDB header:oxidoreductase Chain: A: PDB Molecule:putative bacterioferritin; PDBTitle: crystal structure of putative bacterioferritin from2 campylobacter jejuni 56 d1vela_ not modelled 5.5 12 Fold:Ferritin-like Superfamily:Ferritin-like Family:Ferritin 57 d1j5ya2 not modelled 5.3 12 Fold:HPr-like Superfamily:Putative transcriptional regulator TM1602, C-terminal domain Family:Putative transcriptional regulator TM1602, C-terminal domain 58 d2eiaa1 not modelled 5.1 17 Fold:Acyl carrier protein-like Superfamily:Retrovirus capsid dimerization domain-like Family:Retrovirus capsid protein C-terminal domain 59 c3jzeC_ not modelled 5.0 21 PDB header:hydrolase Chain: C: PDB Molecule:dihydroorotase; PDBTitle: 1.8 angstrom resolution crystal structure of dihydroorotase (pyrc)2 from salmonella enterica subsp. enterica serovar typhimurium str. lt2

Binding site prediction

Due to computational demand, binding site predictions are not run for batch jobs

If you want to predict binding sites, please manually submit your model of choice to 3DLigandSite

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Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
If you use the binding site predictions from 3DLigandSite, please also cite:
3DLigandSite: predicting ligand-binding sites using similar structures.
Wass MN, Kelley LA and Sternberg MJ Nucleic Acids Research 38, W469-73 (2010) [PubMed]
© Structural Bioinformatics Group Imperial College London Lawrence Kelley, Benjamin Jefferys Disclaimer Terms and Conditions	Component software Template detection: HHpred 1.51 Secondary structure prediction: Psi-pred 2.5 Disorder prediction: Disopred 2.4 Transmembrane prediction: Memsat_SVM Multi-template modelling and ab initio: Poing 1.0