Secondary structure and disorder prediction

		1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	K	K	I	L	E	A	I	L	A	I	N	L	A	V	L	S	C	I	V	F	I	N	I	I	L	R	Y	G	F	Q	T	S	I	L	S	V	D	E	L	S	R	Y	L	F	V	W	L	T	F	I	G	A	I	V	A	F	M	D	N
Secondary structure
SS confidence
Disorder		?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		A	H	V	Q	V	T	F	L	V	E	K	L	S	P	A	W	Q	R	R	V	A	L	V	T	H	S	L	I	L	F	I	C	G	A	L	A	W	G	A	T	L	K	T	I	Q	D	W	S	D	Y	S	P	I	L	G	L	P	I	G	L
Secondary structure
SS confidence
Disorder																																																?	?	?	?	?	?	?		?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.
Sequence		M	Y	A	A	C	L	P	T	S	L	V	I	A	F	F	E	L	R	H	L	Y	Q	L	I	T	R	S	N	S	L	T	S	P	P	Q	G	A
Secondary structure
SS confidence
Disorder																													?	?	?	?	?	?	?	?	?	?
Disorder confidence

Confidence Key
High(9)											Low (0)

?	Disordered
	Alpha helix
	Beta strand

Domain analysis

Hover over an aligned region to see model and summary info

Please note, only up to the top 20 hits are modelled to reduce computer load

Rank Aligned region

PDB 2knc chain A Region: 108 - 157 Aligned: 50 Modelled: 50 Confidence: 47.7% Identity: 14% PDB header:cell adhesion Chain: A: PDB Molecule:integrin alpha-iib; PDBTitle: platelet integrin alfaiib-beta3 transmembrane-cytoplasmic2 heterocomplex Phyre2 PDB 1yle chain A domain 1 Region: 21 - 80 Aligned: 41 Modelled: 40 Confidence: 22.7% Identity: 27% Fold: Acyl-CoA N-acyltransferases (Nat) Superfamily: Acyl-CoA N-acyltransferases (Nat) Family: AstA-like Phyre2 PDB 2k42 chain A Region: 52 - 84 Aligned: 33 Modelled: 33 Confidence: 8.5% Identity: 18% PDB header:signaling protein Chain: A: PDB Molecule:wiskott-aldrich syndrome protein; PDBTitle: solution structure of the gtpase binding domain of wasp in2 complex with espfu, an ehec effector Phyre2 PDB 2odb chain B Region: 52 - 81 Aligned: 30 Modelled: 30 Confidence: 7.3% Identity: 23% PDB header:protein binding Chain: B: PDB Molecule:serine/threonine-protein kinase pak 6; PDBTitle: the crystal structure of human cdc42 in complex with the crib domain2 of human p21-activated kinase 6 (pak6) Phyre2 PDB 3k6g chain A Region: 22 - 39 Aligned: 17 Modelled: 18 Confidence: 6.7% Identity: 0% PDB header:protein binding Chain: A: PDB Molecule:telomeric repeat-binding factor 2-interacting protein 1; PDBTitle: crystal structure of rap1 and trf2 complex Phyre2 PDB 2kpa chain A Region: 63 - 79 Aligned: 17 Modelled: 17 Confidence: 6.1% Identity: 29% PDB header:hydrolase Chain: A: PDB Molecule:arno(375-400); PDBTitle: specific motifs of the v-atpase a2-subunit isoform interact2 with catalytic and regulatory domains of arno Phyre2 1 c2kncA_ 2 d1ylea1 3 c2k42A_ 4 c2odbB_ 5 c3k6gA_ 6 c2kpaA_

Detailed template information

# Template Alignment Coverage 3D Model Confidence % i.d. Template Information

1 c2kncA_ 47.7 14 PDB header:cell adhesion Chain: A: PDB Molecule:integrin alpha-iib; PDBTitle: platelet integrin alfaiib-beta3 transmembrane-cytoplasmic2 heterocomplex 2 d1ylea1 22.7 27 Fold:Acyl-CoA N-acyltransferases (Nat) Superfamily:Acyl-CoA N-acyltransferases (Nat) Family:AstA-like 3 c2k42A_ 8.5 18 PDB header:signaling protein Chain: A: PDB Molecule:wiskott-aldrich syndrome protein; PDBTitle: solution structure of the gtpase binding domain of wasp in2 complex with espfu, an ehec effector 4 c2odbB_ 7.3 23 PDB header:protein binding Chain: B: PDB Molecule:serine/threonine-protein kinase pak 6; PDBTitle: the crystal structure of human cdc42 in complex with the crib domain2 of human p21-activated kinase 6 (pak6) 5 c3k6gA_ 6.7 0 PDB header:protein binding Chain: A: PDB Molecule:telomeric repeat-binding factor 2-interacting protein 1; PDBTitle: crystal structure of rap1 and trf2 complex 6 c2kpaA_ 6.1 29 PDB header:hydrolase Chain: A: PDB Molecule:arno(375-400); PDBTitle: specific motifs of the v-atpase a2-subunit isoform interact2 with catalytic and regulatory domains of arno

Binding site prediction

Due to computational demand, binding site predictions are not run for batch jobs

If you want to predict binding sites, please manually submit your model of choice to 3DLigandSite

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Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
If you use the binding site predictions from 3DLigandSite, please also cite:
3DLigandSite: predicting ligand-binding sites using similar structures.
Wass MN, Kelley LA and Sternberg MJ Nucleic Acids Research 38, W469-73 (2010) [PubMed]
© Structural Bioinformatics Group Imperial College London Lawrence Kelley, Benjamin Jefferys Disclaimer Terms and Conditions	Component software Template detection: HHpred 1.51 Secondary structure prediction: Psi-pred 2.5 Disorder prediction: Disopred 2.4 Transmembrane prediction: Memsat_SVM Multi-template modelling and ab initio: Poing 1.0