Secondary structure and disorder prediction

		1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	K	T	T	H	T	S	L	P	F	A	G	H	T	L	H	F	V	E	F	D	P	A	N	F	C	E	Q	D	L	L	W	L	P	H	Y	A	Q	L	Q	H	A	G	R	K	R	K	T	E	H	L	A	G	R	I	A	A	V	Y	A
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?																																		?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		L	R	E	Y	G	Y	K	C	V	P	A	I	G	E	L	R	Q	P	V	W	P	A	E	V	Y	G	S	I	S	H	C	G	T	T	A	L	A	V	V	S	R	Q	P	I	G	I	D	I	E	E	I	F	S	V	Q	T	A	R	E	L
Secondary structure
SS confidence
Disorder												?																																										?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		T	D	N	I	I	T	P	A	E	H	E	R	L	A	D	C	G	L	A	F	S	L	A	L	T	L	A	F	S	A	K	E	S	A	F	K	A	S	E	I	Q	T	D	A	G	F	L	D	Y	Q	I	I	S	W	N	K	Q	Q	V	I
Secondary structure
SS confidence
Disorder																																																									?
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.
Sequence		I	H	R	E	N	E	M	F	A	V	H	W	Q	I	K	E	K	I	V	I	T	L	C	Q	H	D
Secondary structure
SS confidence
Disorder																										?	?
Disorder confidence

Confidence Key
High(9)											Low (0)

?	Disordered
	Alpha helix
	Beta strand

Domain analysis

Hover over an aligned region to see model and summary info

Please note, only up to the top 20 hits are modelled to reduce computer load

Rank Aligned region

PDB 1qr0 chain A Region: 13 - 204 Aligned: 190 Modelled: 192 Confidence: 100.0% Identity: 16% PDB header:transferase Chain: A: PDB Molecule:4'-phosphopantetheinyl transferase sfp; PDBTitle: crystal structure of the 4'-phosphopantetheinyl transferase sfp-2 coenzyme a complex Phyre2 PDB 2cg5 chain A Region: 29 - 206 Aligned: 178 Modelled: 178 Confidence: 100.0% Identity: 14% PDB header:transferase/hydrolase Chain: A: PDB Molecule:l-aminoadipate-semialdehyde dehydrogenase- PDBTitle: structure of aminoadipate-semialdehyde dehydrogenase-2 phosphopantetheinyl transferase in complex with cytosolic3 acyl carrier protein and coenzyme a Phyre2 PDB 1qr0 chain A domain 2 Region: 102 - 206 Aligned: 103 Modelled: 105 Confidence: 99.8% Identity: 19% Fold: 4'-phosphopantetheinyl transferase Superfamily: 4'-phosphopantetheinyl transferase Family: 4'-Phosphopantetheinyl transferase SFP Phyre2 PDB 2was chain A Region: 103 - 203 Aligned: 96 Modelled: 101 Confidence: 99.4% Identity: 23% PDB header:transferase Chain: A: PDB Molecule:3-oxoacyl-[acyl-carrier-protein] synthase; PDBTitle: structure of the fungal type i fas ppt domain Phyre2 PDB 3h88 chain A Region: 103 - 203 Aligned: 100 Modelled: 97 Confidence: 99.4% Identity: 22% PDB header:transferase Chain: A: PDB Molecule:holo-[acyl-carrier-protein] synthase; PDBTitle: crystal structure of 4'-phosphopantetheinyl transferase acps from2 vibrio cholerae o1 biovar eltor Phyre2 PDB 3hyk chain B Region: 103 - 203 Aligned: 101 Modelled: 101 Confidence: 99.4% Identity: 21% PDB header:transferase Chain: B: PDB Molecule:holo-[acyl-carrier-protein] synthase; PDBTitle: 2.31 angstrom resolution crystal structure of a holo-(acyl-carrier-2 protein) synthase from bacillus anthracis str. ames in complex with3 coa (3',5'-adp) Phyre2 PDB 1fth chain A Region: 103 - 203 Aligned: 101 Modelled: 101 Confidence: 99.4% Identity: 24% Fold: 4'-phosphopantetheinyl transferase Superfamily: 4'-phosphopantetheinyl transferase Family: Holo-(acyl carrier protein) synthase ACPS Phyre2 PDB 2jbz chain A Region: 103 - 203 Aligned: 100 Modelled: 101 Confidence: 99.4% Identity: 18% PDB header:transferase Chain: A: PDB Molecule:holo-[acyl-carrier-protein] synthase; PDBTitle: crystal structure of the streptomyces coelicolor holo-2 [acyl-carrier-protein] synthase (acps) in complex with3 coenzyme a at 1.6 a Phyre2 PDB 1f7l chain A Region: 103 - 203 Aligned: 101 Modelled: 101 Confidence: 99.3% Identity: 22% Fold: 4'-phosphopantetheinyl transferase Superfamily: 4'-phosphopantetheinyl transferase Family: Holo-(acyl carrier protein) synthase ACPS Phyre2 PDB 3ne9 chain B Region: 103 - 203 Aligned: 101 Modelled: 101 Confidence: 99.3% Identity: 19% PDB header:transferase Chain: B: PDB Molecule:phosphopantethiene protein transferase, ppt1p; PDBTitle: mycobacterium tuberculosis acyl carrier protein synthase adopts two2 different structural conformations: ph driven conformational changes3 of acps enzyme Phyre2 PDB 1qr0 chain A domain 1 Region: 13 - 101 Aligned: 89 Modelled: 89 Confidence: 99.3% Identity: 12% Fold: 4'-phosphopantetheinyl transferase Superfamily: 4'-phosphopantetheinyl transferase Family: 4'-Phosphopantetheinyl transferase SFP Phyre2 PDB 3f09 chain B Region: 103 - 203 Aligned: 96 Modelled: 101 Confidence: 99.3% Identity: 24% PDB header:transferase Chain: B: PDB Molecule:holo-[acyl-carrier-protein] synthase; PDBTitle: 1.82 angstrom resolution crystal structure of holo-(acyl-carrier-2 protein) synthase (acps) from staphylococcus aureus Phyre2 PDB 2qg8 chain A Region: 104 - 203 Aligned: 100 Modelled: 100 Confidence: 99.1% Identity: 14% PDB header:transferase Chain: A: PDB Molecule:acyl carrier protein synthase py06285; PDBTitle: plasmodium yoelii acyl carrier protein synthase py06285 with adp bound Phyre2 PDB 3hqj chain A Region: 104 - 203 Aligned: 94 Modelled: 100 Confidence: 98.7% Identity: 17% PDB header:transferase Chain: A: PDB Molecule:holo-[acyl-carrier-protein] synthase; PDBTitle: structure-function analysis of mycobacterium tuberculosis2 acyl carrier protein synthase (acps). Phyre2 PDB 2ch4 chain A Region: 54 - 127 Aligned: 72 Modelled: 74 Confidence: 41.9% Identity: 22% PDB header:transferase/chemotaxis Chain: A: PDB Molecule:chemotaxis protein chea; PDBTitle: complex between bacterial chemotaxis histidine kinase chea2 domains p4 and p5 and receptor-adaptor protein chew Phyre2 PDB 1y8o chain A Region: 81 - 126 Aligned: 40 Modelled: 46 Confidence: 36.0% Identity: 10% PDB header:transferase Chain: A: PDB Molecule:[pyruvate dehydrogenase [lipoamide]] kinase isozyme 3; PDBTitle: crystal structure of the pdk3-l2 complex Phyre2 PDB 2q8f chain A Region: 81 - 126 Aligned: 40 Modelled: 46 Confidence: 29.9% Identity: 8% PDB header:transferase Chain: A: PDB Molecule:[pyruvate dehydrogenase [lipoamide]] kinase isozyme 1; PDBTitle: structure of pyruvate dehydrogenase kinase isoform 1 Phyre2 PDB 3d2r chain B Region: 81 - 126 Aligned: 40 Modelled: 46 Confidence: 23.5% Identity: 8% PDB header:transferase Chain: B: PDB Molecule:[pyruvate dehydrogenase [lipoamide]] kinase isozyme 4; PDBTitle: crystal structure of pyruvate dehydrogenase kinase isoform 4 in2 complex with adp Phyre2 PDB 2bu8 chain A Region: 81 - 126 Aligned: 40 Modelled: 46 Confidence: 21.6% Identity: 8% PDB header:transferase Chain: A: PDB Molecule:pyruvate dehydrogenase kinase isoenzyme 2; PDBTitle: crystal structures of human pyruvate dehydrogenase kinase 22 containing physiological and synthetic ligands Phyre2 PDB 1hq0 chain A Region: 195 - 206 Aligned: 12 Modelled: 12 Confidence: 16.1% Identity: 25% Fold: CNF1/YfiH-like putative cysteine hydrolases Superfamily: CNF1/YfiH-like putative cysteine hydrolases Family: Type 1 cytotoxic necrotizing factor, catalytic domain Phyre2 1 c1qr0A_ 2 c2cg5A_ 3 d1qr0a2 4 c2wasA_ 5 c3h88A_ 6 c3hykB_ 7 d1ftha_ 8 c2jbzA_ 9 d1f7la_ 10 c3ne9B_ 11 d1qr0a1 12 c3f09B_ 13 c2qg8A_ 14 c3hqjA_ 15 c2ch4A_ 16 c1y8oA_ 17 c2q8fA_ 18 c3d2rB_ 19 c2bu8A_ 20 d1hq0a_ 21 22 23 24 25 26 27 28 29

Detailed template information

# Template Alignment Coverage 3D Model Confidence % i.d. Template Information

1 c1qr0A_ 100.0 16 PDB header:transferase Chain: A: PDB Molecule:4'-phosphopantetheinyl transferase sfp; PDBTitle: crystal structure of the 4'-phosphopantetheinyl transferase sfp-2 coenzyme a complex 2 c2cg5A_ 100.0 14 PDB header:transferase/hydrolase Chain: A: PDB Molecule:l-aminoadipate-semialdehyde dehydrogenase- PDBTitle: structure of aminoadipate-semialdehyde dehydrogenase-2 phosphopantetheinyl transferase in complex with cytosolic3 acyl carrier protein and coenzyme a 3 d1qr0a2 99.8 19 Fold:4'-phosphopantetheinyl transferase Superfamily:4'-phosphopantetheinyl transferase Family:4'-Phosphopantetheinyl transferase SFP 4 c2wasA_ 99.4 23 PDB header:transferase Chain: A: PDB Molecule:3-oxoacyl-[acyl-carrier-protein] synthase; PDBTitle: structure of the fungal type i fas ppt domain 5 c3h88A_ 99.4 22 PDB header:transferase Chain: A: PDB Molecule:holo-[acyl-carrier-protein] synthase; PDBTitle: crystal structure of 4'-phosphopantetheinyl transferase acps from2 vibrio cholerae o1 biovar eltor 6 c3hykB_ 99.4 21 PDB header:transferase Chain: B: PDB Molecule:holo-[acyl-carrier-protein] synthase; PDBTitle: 2.31 angstrom resolution crystal structure of a holo-(acyl-carrier-2 protein) synthase from bacillus anthracis str. ames in complex with3 coa (3',5'-adp) 7 d1ftha_ 99.4 24 Fold:4'-phosphopantetheinyl transferase Superfamily:4'-phosphopantetheinyl transferase Family:Holo-(acyl carrier protein) synthase ACPS 8 c2jbzA_ 99.4 18 PDB header:transferase Chain: A: PDB Molecule:holo-[acyl-carrier-protein] synthase; PDBTitle: crystal structure of the streptomyces coelicolor holo-2 [acyl-carrier-protein] synthase (acps) in complex with3 coenzyme a at 1.6 a 9 d1f7la_ 99.3 22 Fold:4'-phosphopantetheinyl transferase Superfamily:4'-phosphopantetheinyl transferase Family:Holo-(acyl carrier protein) synthase ACPS 10 c3ne9B_ 99.3 19 PDB header:transferase Chain: B: PDB Molecule:phosphopantethiene protein transferase, ppt1p; PDBTitle: mycobacterium tuberculosis acyl carrier protein synthase adopts two2 different structural conformations: ph driven conformational changes3 of acps enzyme 11 d1qr0a1 99.3 12 Fold:4'-phosphopantetheinyl transferase Superfamily:4'-phosphopantetheinyl transferase Family:4'-Phosphopantetheinyl transferase SFP 12 c3f09B_ 99.3 24 PDB header:transferase Chain: B: PDB Molecule:holo-[acyl-carrier-protein] synthase; PDBTitle: 1.82 angstrom resolution crystal structure of holo-(acyl-carrier-2 protein) synthase (acps) from staphylococcus aureus 13 c2qg8A_ 99.1 14 PDB header:transferase Chain: A: PDB Molecule:acyl carrier protein synthase py06285; PDBTitle: plasmodium yoelii acyl carrier protein synthase py06285 with adp bound 14 c3hqjA_ 98.7 17 PDB header:transferase Chain: A: PDB Molecule:holo-[acyl-carrier-protein] synthase; PDBTitle: structure-function analysis of mycobacterium tuberculosis2 acyl carrier protein synthase (acps). 15 c2ch4A_ 41.9 22 PDB header:transferase/chemotaxis Chain: A: PDB Molecule:chemotaxis protein chea; PDBTitle: complex between bacterial chemotaxis histidine kinase chea2 domains p4 and p5 and receptor-adaptor protein chew 16 c1y8oA_ 36.0 10 PDB header:transferase Chain: A: PDB Molecule:[pyruvate dehydrogenase [lipoamide]] kinase isozyme 3; PDBTitle: crystal structure of the pdk3-l2 complex 17 c2q8fA_ 29.9 8 PDB header:transferase Chain: A: PDB Molecule:[pyruvate dehydrogenase [lipoamide]] kinase isozyme 1; PDBTitle: structure of pyruvate dehydrogenase kinase isoform 1 18 c3d2rB_ 23.5 8 PDB header:transferase Chain: B: PDB Molecule:[pyruvate dehydrogenase [lipoamide]] kinase isozyme 4; PDBTitle: crystal structure of pyruvate dehydrogenase kinase isoform 4 in2 complex with adp 19 c2bu8A_ 21.6 8 PDB header:transferase Chain: A: PDB Molecule:pyruvate dehydrogenase kinase isoenzyme 2; PDBTitle: crystal structures of human pyruvate dehydrogenase kinase 22 containing physiological and synthetic ligands 20 d1hq0a_ 16.1 25 Fold:CNF1/YfiH-like putative cysteine hydrolases Superfamily:CNF1/YfiH-like putative cysteine hydrolases Family:Type 1 cytotoxic necrotizing factor, catalytic domain 21 d1xhna1 not modelled 8.2 22 Fold:Split barrel-like Superfamily:FMN-binding split barrel Family:PNP-oxidase like 22 d1pv0a_ not modelled 6.3 47 Fold:Long alpha-hairpin Superfamily:Sporulation inhibitor Sda Family:Sporulation inhibitor Sda 23 c3pmiC_ not modelled 6.3 33 PDB header:protein binding Chain: C: PDB Molecule:pwwp domain-containing protein mum1; PDBTitle: pwwp domain of human mutated melanoma-associated antigen 1 24 d1jmxa3 not modelled 6.0 17 Fold:Immunoglobulin-like beta-sandwich Superfamily:E set domains Family:Quinohemoprotein amine dehydrogenase A chain, domains 4 and 5 25 d1i58a_ not modelled 5.9 15 Fold:ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase Superfamily:ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase Family:Histidine kinase 26 d2f1da2 not modelled 5.9 50 Fold:Ribosomal protein S5 domain 2-like Superfamily:Ribosomal protein S5 domain 2-like Family:Imidazole glycerol phosphate dehydratase 27 d2ae8a2 not modelled 5.6 50 Fold:Ribosomal protein S5 domain 2-like Superfamily:Ribosomal protein S5 domain 2-like Family:Imidazole glycerol phosphate dehydratase 28 c1wnmA_ not modelled 5.5 50 PDB header:transcription Chain: A: PDB Molecule:fibroin-modulator binding-protein-1; PDBTitle: nmr structure of fmbp-1 tandem repeat 2 in 30%(v/v) tfe2 solution 29 d1pbya3 not modelled 5.4 20 Fold:Immunoglobulin-like beta-sandwich Superfamily:E set domains Family:Quinohemoprotein amine dehydrogenase A chain, domains 4 and 5

Binding site prediction

Due to computational demand, binding site predictions are not run for batch jobs

If you want to predict binding sites, please manually submit your model of choice to 3DLigandSite

Transmembrane helix prediction

Transmembrane helices have been predicted in your sequence to adopt the topology shown below

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Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
If you use the binding site predictions from 3DLigandSite, please also cite:
3DLigandSite: predicting ligand-binding sites using similar structures.
Wass MN, Kelley LA and Sternberg MJ Nucleic Acids Research 38, W469-73 (2010) [PubMed]
© Structural Bioinformatics Group Imperial College London Lawrence Kelley, Benjamin Jefferys Disclaimer Terms and Conditions	Component software Template detection: HHpred 1.51 Secondary structure prediction: Psi-pred 2.5 Disorder prediction: Disopred 2.4 Transmembrane prediction: Memsat_SVM Multi-template modelling and ab initio: Poing 1.0