Job Description P08201 Confidence 100.00% Date Thu Jan 5 11:00:55 GMT 2012 Rank 50 Aligned Residues 390 % Identity 19% Template c1geuA_ PDB info PDB header:oxidoreductase(flavoenzyme) Chain: A: PDB Molecule:glutathione reductase; PDBTitle: anatomy of an engineered nad-binding site Resolution 2.20 Å

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		2	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.																											.	.	.	.	.	.	.	50	.	.	.	.	.
Predicted Secondary structure																																											.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.
Query SS confidence																																											.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.
Query Sequence		S	K	V	R	L	A	I	I	G	N	G	M	V	G	H	R	F	I	E	D	L	L	D	K	S	D	A	A	N	F	D	I	T	V	F	C	E	E	P	R	I	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	A	Y	D	R	V	H	L	S	S	Y	F	S	H
Query Conservation																																											.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.
Alig confidence																											.	.	.	.													.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.
Template Conservation																											.	.	.	.
Template Sequence		K	H	Y	D	Y	I	A	I	G	G	G	S	G	G	I	A	S	I	N	R	A	A	M	Y	G	.	.	.	.	Q	K	C	A	L	I	E	A	K	E	L	G	G	T	C	V	N	V	G	C	V	P	K	K	V	M	W	H	A	A	Q	I	R	E	A	I	H	M	Y	G	P	D	Y	G	F	D	T	T	I	N	K
Template Known Secondary structure												S												T	T	T	.	.	.	.								S	S		T	T							S																			T	T	T	G	G	G	T
Template Predicted Secondary structure																											.	.	.	.
Template SS confidence
		3	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.					.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60	.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.
		56	.	.	.	60	.	.	.	.												.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120	.	.	.	.
Predicted Secondary structure											.	.	.	.	.	.	.	.	.	.	.
Query SS confidence											.	.	.	.	.	.	.	.	.	.	.
Query Sequence		H	T	A	E	E	L	S	L	V	.	.	.	.	.	.	.	.	.	.	.	R	E	G	F	Y	E	K	H	G	I	K	V	L	V	G	E	R	A	I	T	I	N	R	Q	E	K	V	I	H	S	S	A	G	R	T	V	F	Y	D	K	L	I	M	A	T	G	S	Y	P	W	I	P	P	I	K	G	S	D	T	Q
Query Conservation											.	.	.	.	.	.	.	.	.	.	.
Alig confidence											.	.	.	.	.	.	.	.	.	.	.																					.	.	.	.																																				.
Template Conservation																																										.	.	.	.																																				.
Template Sequence		F	N	W	E	T	L	I	A	S	R	T	A	Y	I	D	R	I	H	T	S	Y	E	N	V	L	G	K	N	N	V	D	V	I	K	G	F	A	R	F	V	.	.	.	.	D	A	K	T	L	E	V	N	G	E	T	I	T	A	D	H	I	L	I	A	T	G	G	R	P	S	H	P	D	I	P	G	V	E	Y	.
Template Known Secondary structure																													T	T						S						.	.	.	.		T	T					T	T																					S	T	T	G	G	G	.
Template Predicted Secondary structure																																										.	.	.	.																																				.
Template SS confidence
		79	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.					.	120	.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.
		125	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180	.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.
Predicted Secondary structure
Query SS confidence
Query Sequence		D	C	F	V	Y	R	T	I	E	D	L	N	A	I	E	S	C	A	R	R	S	K	R	G	A	V	V	G	G	G	L	L	G	L	E	A	A	G	A	L	K	N	L	G	I	E	T	H	V	I	E	F	A	P	M	L	M	A	E	Q	L	D	Q	M	G	G	E	Q	L	R	R	K	I	E	S	M	G	V	R	V
Query Conservation
Alig confidence		.	.	.	.	.	.	.																																																					.
Template Conservation		.	.	.	.	.	.	.																																																					.
Template Sequence		.	.	.	.	.	.	.	G	I	D	S	D	G	F	F	A	L	P	A	L	P	E	R	V	A	V	V	G	A	G	Y	I	G	V	E	L	G	G	V	I	N	G	L	G	A	K	T	H	L	F	E	M	F	D	A	P	L	P	S	.	F	D	P	M	I	S	E	T	L	V	E	V	M	N	A	E	G	P	Q	L
Template Known Secondary structure		.	.	.	.	.	.	.	S	B							T		S	S			S								S													T	T								S	S	S	S	S	S	T	T	.	S
Template Predicted Secondary structure		.	.	.	.	.	.	.																																																					.
Template SS confidence
									154	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180	.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.		.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.
		205	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240	.	.	.	.	.	.	.	.	.			250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.
Predicted Secondary structure																																															.	.
Query SS confidence																																															.	.
Query Sequence		H	T	S	K	N	T	L	E	I	V	Q	E	G	V	E	A	R	K	T	M	R	F	A	D	G	S	E	L	E	V	D	F	I	V	F	S	T	G	I	R	P	R	D	K	L	.	.	A	T	Q	C	G	L	D	V	A	P	R	G	G	I	V	I	N	D	S	C	Q	T	S	D	P	D	I	Y	A	I	G	E	C
Query Conservation																																															.	.
Alig confidence																				.																											.	.
Template Conservation																				.
Template Sequence		H	T	N	A	I	P	K	A	V	V	K	N	T	D	G	S	L	T	.	L	E	L	E	D	G	R	S	E	T	V	D	C	L	I	W	A	I	G	R	E	P	A	N	D	N	I	N	L	E	A	A	G	V	K	T	N	E	K	G	Y	I	V	V	D	K	Y	Q	N	T	N	I	E	G	I	Y	A	V	G	D	N
Template Known Secondary structure				S										T	T	S				.				T	T	S						S						S							S	T	T						T			B		T	T	S		B				T	T	S	B		S	S	T	T					G	G	G
Template Predicted Secondary structure																				.
Template SS confidence
		226	.	.	.	230	.	.	.	.	.	.	.	.	.	240	.	.	.		.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300	.	.	.	.
		283	.	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300	.	.	.	.	.	.	.	.	.	310	.	.	.	.	.	.	.	.	.	320	.		.	.	.	.	.	.	.	.	330	.	.	.	.	.	.	.	.	.					340	.	.	.	.	.	.	.	.	.	350	.	.	.	.	.	.	.
Predicted Secondary structure																																									.																			.	.	.	.
Query SS confidence																																									.																			.	.	.	.
Query Sequence		A	S	W	N	N	R	V	F	G	L	V	A	P	G	Y	K	M	A	Q	V	A	V	D	H	I	L	G	S	E	N	A	F	E	G	A	D	L	S	A	.	K	L	K	L	L	G	V	D	V	G	G	I	G	D	A	H	G	R	.	.	.	.	T	P	G	A	R	S	Y	V	Y	L	D	E	S	K	E	I	Y	K
Query Conservation																																									.																			.	.	.	.
Alig confidence				.	.	.	.																																		.																			.	.	.	.
Template Conservation				.	.	.	.
Template Sequence		T	G	.	.	.	.	A	V	E	L	T	P	V	A	V	A	A	G	R	R	L	S	E	R	L	F	N	N	K	P	D	E	H	L	D	Y	S	N	I	P	T	V	V	F	S	H	P	P	I	G	T	V	G	L	T	E	P	Q	A	R	E	Q	Y	G	D	D	Q	V	K	V	Y	K	S	S	F	T	A	M	Y	T
Template Known Secondary structure		G	T	.	.	.	.	S																				T	T		T	T						S	S								S	S																		G	G	G
Template Predicted Secondary structure				.	.	.	.
Template SS confidence
		305	.					.	.	.	310	.	.	.	.	.	.	.	.	.	320	.	.	.	.	.	.	.	.	.	330	.	.	.	.	.	.	.	.	.	340	.	.	.	.	.	.	.	.	.	350	.	.	.	.	.	.	.	.	.	360	.	.	.	.	.	.	.	.	.	370	.	.	.	.	.	.	.	.	.	380
													358	.	360	.	.	.	.	.	.	.	.	.	370	.	.	.	.	.	.			.	.	.	380	.	.	.	.	.	.	.	.	.	390	.	.	.	.	.	.	.	.	.	400	.	.	.	.	.	.	.	.	.	410	.	.	.
Predicted Secondary structure		.	.	.	.	.	.	.	.	.	.	.																				.	.
Query SS confidence		.	.	.	.	.	.	.	.	.	.	.																				.	.
Query Sequence		.	.	.	.	.	.	.	.	.	.	.	R	L	I	V	S	E	D	N	K	T	L	L	G	A	V	L	V	G	D	.	.	T	S	D	Y	G	N	L	L	Q	L	V	L	N	A	I	E	L	P	E	N	P	D	S	L	I	L	P	A	H	S	G	S	G	K	P	S	I
Query Conservation		.	.	.	.	.	.	.	.	.	.	.																				.	.
Alig confidence		.	.	.	.	.	.	.	.	.	.	.																				.	.
Template Conservation
Template Sequence		A	V	T	T	H	R	Q	P	C	R	M	K	L	V	C	V	G	S	E	E	K	I	V	G	I	H	G	I	G	F	G	M	D	E	M	L	Q	G	F	A	V	A	L	K	M	G	A	T	K	K	D	F	D	N	T	V	A	I	H	P	T	A	A	E	E	F	V	T	M
Template Known Secondary structure			T	S	S	S												T	T	T	T									S	T	T														T	T		B						T	S				S	S		S	G	G	G	G	G	T
Template Predicted Secondary structure
Template SS confidence
		381	.	.	.	.	.	.	.	.	390	.	.	.	.	.	.	.	.	.	400	.	.	.	.	.	.	.	.	.	410	.	.	.	.	.	.	.	.	.	420	.	.	.	.	.	.	.	.	.	430	.	.	.	.	.	.	.	.	.	440	.	.	.	.	.	.	.	.	.