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Job DescriptionP33937
Confidence100.00%DateThu Jan 5 11:52:56 GMT 2012
Rank3Aligned Residues785
% Identity22%Templatec1kqgA_
PDB info PDB header:oxidoreductaseChain: A: PDB Molecule:formate dehydrogenase, nitrate-inducible, major subunit; PDBTitle: formate dehydrogenase n from e. coli
Resolution2.80 Å

  Insertion relative to template
  Deletion relative to template
  Catalytic residue from the CSA
 
Detailed help on interpreting your alignment


   30.........40.........50.........60. ........70.........80.........90.........100..
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Query Sequence  RAVVGQQEAIKWDKAPCRFCGTGCGVLVGTQQ. . . . . . . GRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
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Template Conservation            
 
 
 
  
   

  
 
   
     
 

 
 


  
 
 
  
 

 
     
 
 

  


Template Sequence  QARNYKLLRAKEIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYVNSENRLRYPEY
Template Known Secondary structure 




TTTTS
SSBTT

S
S
TTS


TT
TTTTT


TTT
TTB

S
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   34.....40.........50.........60.........70.........80.........90.........100.........110...
 
   103......110.........120.........130.........140.. .......150.........160.........170
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Query Sequence  RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPES. . . . . . . . . . . . IGMFGSGQWTIWEGYAASKLFKAGFRSN
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Template Sequence  RAP. . . . . GSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLSTGMLCASGASNETGMLTQKFARS. LGML
Template Known Secondary structure 
T.....T
SS




TTS
B
S
TTS
.TTB
Template Predicted Secondary structure 
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   114.. ...120.........130.........140.........150.........160.........170.........180... ....
 
   171........180.........190.........200.........210.........220.........230.........240.........250
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Query Sequence  NIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAEMHPILWSRITNRRLSNQNVTVAVLSTYQHRSF
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Template Conservation 
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Template Sequence  AVDNQAR. VHGPTVASLAPTFGRGAMTNHWVDIKNANVVMVMGGNAAEAHPVGFRWAMEA. KNNNDATLIVVDPRFTRTA
Template Known Secondary structure 
GGG.

S

S
SS
TGGGGG
SS

S.S


SS

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   188.190.... .....200.........210.........220.........230.........240...... ...250.........260.....
 
   251........260.........270.........280.........290.........300....... ..310.
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Query Sequence  ELADNGIIFTPQSDLVILNYIANYIIQNNAINQDFFSKHVNLRKGATDIGYGLRPTH. . . . . . . . . . . . . . . . . . . PLEK
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Template Sequence  SVADIYAPIRSGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYDKSSWNYQLDENG
Template Known Secondary structure  T
S


TT
TT
S
BSTTBBB
TT

TTTTTTTT

TTSSB
B
TTS
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   267..270.........280.........290.........300.........310.........320.........330.........340......
 
   312.......320.........330.........340.........350.........360... ......370.........380........
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Query Sequence  AAKNPGSDASEPMSFEDYKAFVAEYTLEKTAEMTGVPKDQLEQLAQLYADPN. . . KKVISYWTMGFNQHTRGVWANNLVY
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Template Conservation              
 
  
      



 

 






 
  

   
          
    
     

     
  
Template Sequence  YAKRDETLTHPRCVWNLLKEHVSRYTPDVVENICGTPKADFLKVCEVLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMA
Template Known Secondary structure  SB
B
TTS
STTBTT




GGGSTT

SSSTT
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   347..350.........360.........370.........380.........390.........400.........410.........420......
 
   389390.........400.........410.........420.........430.........440.........450.......
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Query Sequence  NLHLLTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPSGTIPAKI. . . . . . . . . . .
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Template Conservation   
 

 


  



     
  
  
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Template Sequence  MIQLLLGNMGMAGGGVNALRGHSNIQGL. TDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFF
Template Known Secondary structure  TT
TTSTT
S


BTT.TT
STTGGG

BTT

SS


SSSS


GGGG
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   427..430.........440.........450.... .....460.........470.........480.........490.........500.....
 
   458.460.........470.........480.........490.........500.........
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Query Sequence  . . . . . . . . . . . . . . . . . . . . . . . . . . . . GLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVSD
Query Conservation  ............................             
 

       

                 

   
  
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Template Conservation                                             

       

  
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Template Sequence  VSLMKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDKNKVV. . SCLSKLKYMVVID
Template Known Secondary structure  GGG

GGGGGGGGGS

BSS


TT


S

SS
..TT
S
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   506...510.........520.........530.........540.........550.........560.........570 .........580...
 
   510.........520 .........530.........540.........550.........560.........570
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Query Sequence  PYPTVSALAAD. . . . . . . . . . . . . . . . . LILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFSRRFK. .
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Template Sequence  PLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDGSIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLREL
Template Known Secondary structure  SB

TGGGTTS

GGGG


GGG


B

GGGS

TTS


S

STT
B
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   584.....590.........600.........610.........620.........630.........640.........650.........660...
 
   571........580.........590.........600.........610.........620......
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Query Sequence  . . . . . . TEEVWPEDLLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFYLQ. . . . . . . . . . . . . . . . . .
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Template Conservation                                                                    
             
Template Sequence  YQSEGGKGVEPLMKMSWNYKQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYTGSW
Template Known Secondary structure 

SSS



SSTTS


SS

TTS

TT
B
S
GGG

SSSS
S
GGGTTS
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   664.....670.........680.........690.........700.........710.........720.........730.........740...
 
   627..630.........640.........650.........660...
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Query Sequence  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . KGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNG
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Template Conservation                                                                              

 
Template Sequence  TEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLIQWNGSKWTGNDIPDFGNAAPGTPTG
Template Known Secondary structure  TTGGG
B



TTSSS

TTBSTTTT
SSTTGGGGB
TTS
BSSGGG


SSSSS

SS
S

TTSS

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   744.....750.........760.........770.........780.........790.........800.........810.........820...
 
   664.....670 .........680.........690.........700.........710.........720.......
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Query Sequence  KETQWRY. . . . . . . . . . . . . . . . SEGNDPYVKAGEGYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHT
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Template Conservation 
                      
   

   
                               


 
 
 
     

Template Sequence  PFIMQPEGMGRLFAINKMAEGPFPEHYEPIETPLGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHT
Template Known Secondary structure  S
TTSTTSSB
SS
TT
STT
SS





SS
TTSS
TT
TT

S
TT



TT


TTT



TT

TT
Template Predicted Secondary structure 



































































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   824.....830.........840.........850.........860.........870.........880.........890.........900...
 
   728.730.........740.........750.........760.........770.........780....... ..790.......
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Query Sequence  GSMTRRVPELHRAFPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQ. . . . . . GLVYMPFFDA. . . .
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Template Sequence  . . WTKHALLNAIAQPEQFVEISETLAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGIPIHWGFEGV
Template Known Secondary structure  ..TGGGSS
S

T

TT
TT
TTS

TT




SSSS
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   904.....910.........920.........930.........940.........950.........960.........970.........980.
 
   798.800.........810.........820.......
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Query Sequence  . . . AQLVNKLTLDATDPLSKETDFKKCAVKLEK
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Template Conservation     
   
 

    

 

        
 
 
Template Sequence  ARKGYIANTLTPNVGDANSQTPEYKAFLVNIEK
Template Known Secondary structure  S




GGGS

S
B
TTT

B
TTS
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   982.......990.........1000.........1010....
 
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Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
 
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Lawrence Kelley, Benjamin Jefferys 
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