Secondary structure and disorder prediction

		1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	K	T	I	F	R	Y	I	L	F	L	A	L	Y	S	C	C	N	T	V	S	A	Y	T	S	F	I	V	G	N	N	A	G	V	D	N	Y	R	G	P	S	T	A	A	Q	M	T	F	N	Y	T	S	T	A	S	N	L	V	F	Y
Secondary structure
SS confidence
Disorder		?	?	?																														?	?	?	?	?	?	?	?	?	?																		?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		K	P	T	Q	L	G	P	T	G	V	K	M	Y	W	S	Y	L	D	T	G	T	G	G	G	I	L	Y	C	N	T	S	G	R	A	N	P	G	P	I	T	I	E	N	A	M	V	Y	S	G	K	D	Y	G	G	H	K	L	F	N	T
Secondary structure
SS confidence
Disorder		?	?	?									?							?		?	?											?	?	?	?											?	?	?			?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		S	V	P	G	L	Y	Y	T	M	L	I	S	R	V	W	S	A	Y	D	T	I	T	D	I	Q	S	P	G	I	Y	I	G	D	P	S	N	Q	E	F	F	F	S	V	T	D	S	D	L	Q	T	K	G	C	N	K	A	D	D	Y	D
Secondary structure
SS confidence
Disorder																?	?	?	?		?	?	?	?	?	?	?	?	?			?														?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		K	F	W	A	I	G	G	I	V	H	N	I	T	V	E	F	Y	T	D	T	N	F	D	P	T	L	N	Q	Q	V	Q	L	S	S	S	S	N	Y	L	Y	S	F	K	A	Y	S	P	G	T	K	V	V	D	H	S	N	H	I	Y	V
Secondary structure
SS confidence
Disorder																									?		?	?	?	?	?	?	?	?	?	?	?													?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300
Sequence		N	F	T	L	N	N	V	K	L	T	L	P	T	C	F	T	S	I	L	T	G	P	S	V	N	G	S	T	V	R	M	G	E	Y	S	S	G	T	I	K	N	G	A	S	P	V	P	F	D	I	S	L	Q	N	C	I	R	V	R	N
Secondary structure
SS confidence
Disorder						?	?	?					?		?	?					?	?	?	?	?	?	?	?													?	?	?	?												?
Disorder confidence
		.	.	.	.	.	.	.	.	.	310	.	.	.	.	.	.	.	.	.	320	.	.	.	.	.	.	.	.	.	330	.	.	.	.	.	.	.	.	.	340	.	.	.	.	.	.	.	.	.	350	.	.	.	.	.	.	.	.	.	360
Sequence		I	E	T	K	L	V	T	G	K	V	G	T	Q	N	T	Q	L	L	G	N	T	L	T	G	S	T	A	A	K	G	V	G	V	L	I	E	G	L	A	T	S	K	N	P	L	M	T	L	K	P	N	D	T	N	S	V	Y	I	D	Y
Secondary structure
SS confidence
Disorder							?	?	?	?	?			?	?					?	?	?	?	?	?	?	?	?	?	?								?	?	?	?	?	?	?	?	?				?				?		?
Disorder confidence
		.	.	.	.	.	.	.	.	.	370	.	.	.	.	.	.	.	.	.	380	.	.	.	.	.	.	.	.	.	390	.	.	.	.	.	.	.	.	.	400	.	.	.	.	.	.	.	.	.	410	.	.
Sequence		E	T	E	D	D	T	S	D	G	V	Y	P	N	Q	G	N	G	T	S	Q	P	L	H	F	Q	A	T	L	K	Q	D	G	N	I	A	I	E	P	G	E	F	K	A	T	S	T	F	Q	V	T	Y	P
Secondary structure
SS confidence
Disorder				?		?	?	?	?	?	?	?	?	?	?	?	?	?		?												?	?	?						?	?											?	?
Disorder confidence

Confidence Key
High(9)											Low (0)

?	Disordered
	Alpha helix
	Beta strand

Domain analysis

Hover over an aligned region to see model and summary info

Please note, only up to the top 20 hits are modelled to reduce computer load

Rank Aligned region

PDB 1klf chain P Region: 118 - 411 Aligned: 208 Modelled: 234 Confidence: 99.8% Identity: 21% PDB header:chaperone/adhesin complex Chain: P: PDB Molecule:fimh protein; PDBTitle: fimh adhesin-fimc chaperone complex with d-mannose Phyre2 PDB 2jty chain A Region: 245 - 411 Aligned: 142 Modelled: 148 Confidence: 99.8% Identity: 25% PDB header:structural protein Chain: A: PDB Molecule:type-1 fimbrial protein, a chain; PDBTitle: self-complemented variant of fima, the main subunit of type 1 pilus Phyre2 PDB 1pdk chain B Region: 249 - 411 Aligned: 140 Modelled: 146 Confidence: 99.8% Identity: 20% Fold: Common fold of diphtheria toxin/transcription factors/cytochrome f Superfamily: Bacterial adhesins Family: Pilus subunits Phyre2 PDB 3jwn chain K Region: 244 - 411 Aligned: 145 Modelled: 151 Confidence: 99.8% Identity: 21% PDB header:protein binding/cell adhesion Chain: K: PDB Molecule:protein fimf; PDBTitle: complex of fimc, fimf, fimg and fimh Phyre2 PDB 3jwn chain L Region: 244 - 411 Aligned: 145 Modelled: 151 Confidence: 99.8% Identity: 21% PDB header:protein binding/cell adhesion Chain: L: PDB Molecule:protein fimf; PDBTitle: complex of fimc, fimf, fimg and fimh Phyre2 PDB 3jwn chain E Region: 244 - 411 Aligned: 145 Modelled: 151 Confidence: 99.8% Identity: 21% PDB header:protein binding/cell adhesion Chain: E: PDB Molecule:protein fimf; PDBTitle: complex of fimc, fimf, fimg and fimh Phyre2 PDB 3jwn chain F Region: 244 - 411 Aligned: 145 Modelled: 151 Confidence: 99.8% Identity: 21% PDB header:protein binding/cell adhesion Chain: F: PDB Molecule:protein fimf; PDBTitle: complex of fimc, fimf, fimg and fimh Phyre2 PDB 3bfw chain A Region: 251 - 411 Aligned: 128 Modelled: 141 Confidence: 99.8% Identity: 17% PDB header:structural protein/structural protein Chain: A: PDB Molecule:protein fimg; PDBTitle: crystal structure of truncated fimg (fimgt) in complex with the donor2 strand peptide of fimf (dsf) Phyre2 PDB 3bwu chain F Region: 260 - 411 Aligned: 126 Modelled: 135 Confidence: 99.8% Identity: 25% PDB header:chaperone, structural, membrane protein Chain: F: PDB Molecule:protein fimf; PDBTitle: crystal structure of the ternary complex of fimd (n-terminal domain,2 fimdn) with fimc and the n-terminally truncated pilus subunit fimf3 (fimft) Phyre2 PDB 2j2z chain B domain 1 Region: 244 - 411 Aligned: 142 Modelled: 147 Confidence: 99.8% Identity: 18% Fold: Common fold of diphtheria toxin/transcription factors/cytochrome f Superfamily: Bacterial adhesins Family: Pilus subunits Phyre2 PDB 1ze3 chain H domain 1 Region: 252 - 411 Aligned: 120 Modelled: 138 Confidence: 99.7% Identity: 18% Fold: Common fold of diphtheria toxin/transcription factors/cytochrome f Superfamily: Bacterial adhesins Family: Pilus subunits Phyre2 PDB 2jmr chain A Region: 245 - 411 Aligned: 144 Modelled: 150 Confidence: 99.7% Identity: 22% PDB header:cell adhesion Chain: A: PDB Molecule:fimf; PDBTitle: nmr structure of the e. coli type 1 pilus subunit fimf Phyre2 PDB 2uy6 chain B domain 1 Region: 244 - 411 Aligned: 143 Modelled: 143 Confidence: 99.7% Identity: 21% Fold: Common fold of diphtheria toxin/transcription factors/cytochrome f Superfamily: Bacterial adhesins Family: Pilus subunits Phyre2 PDB 2w07 chain B Region: 248 - 411 Aligned: 120 Modelled: 133 Confidence: 99.7% Identity: 20% PDB header:cell adhesion Chain: B: PDB Molecule:minor pilin subunit papf; PDBTitle: structural determinants of polymerization reactivity of the2 p pilus adaptor subunit papf Phyre2 PDB 1n12 chain A Region: 252 - 411 Aligned: 135 Modelled: 146 Confidence: 99.3% Identity: 16% Fold: Common fold of diphtheria toxin/transcription factors/cytochrome f Superfamily: Bacterial adhesins Family: Pilus subunits Phyre2 PDB 2wmp chain B Region: 268 - 412 Aligned: 112 Modelled: 116 Confidence: 51.6% Identity: 17% PDB header:chaperone Chain: B: PDB Molecule:papg protein; PDBTitle: structure of the e. coli chaperone papd in complex with the pilin2 domain of the papgii adhesin Phyre2 PDB 3cl5 chain A Region: 45 - 68 Aligned: 23 Modelled: 23 Confidence: 15.2% Identity: 26% PDB header:hydrolase Chain: A: PDB Molecule:hemagglutinin-esterase; PDBTitle: structure of coronavirus hemagglutinin-esterase in complex with 4,9-o-2 diacetyl sialic acid Phyre2 PDB 3nqn chain B Region: 378 - 400 Aligned: 23 Modelled: 23 Confidence: 7.6% Identity: 13% PDB header:structural genomics, unknown function Chain: B: PDB Molecule:uncharacterized protein; PDBTitle: crystal structure of a protein with unknown function. (dr_2006) from2 deinococcus radiodurans at 1.88 a resolution Phyre2 PDB 3c7b chain B domain 3 Region: 253 - 298 Aligned: 43 Modelled: 46 Confidence: 7.3% Identity: 21% Fold: Nitrite and sulphite reductase 4Fe-4S domain-like Superfamily: Nitrite and sulphite reductase 4Fe-4S domain-like Family: Nitrite and sulphite reductase 4Fe-4S domain-like Phyre2 PDB 2akj chain A domain 4 Region: 285 - 298 Aligned: 14 Modelled: 14 Confidence: 6.6% Identity: 7% Fold: Nitrite and sulphite reductase 4Fe-4S domain-like Superfamily: Nitrite and sulphite reductase 4Fe-4S domain-like Family: Nitrite and sulphite reductase 4Fe-4S domain-like Phyre2 1 c1klfP_ 2 c2jtyA_ 3 d1pdkb_ 4 c3jwnK_ 5 c3jwnL_ 6 c3jwnE_ 7 c3jwnF_ 8 c3bfwA_ 9 c3bwuF_ 10 d2j2zb1 11 d1ze3h1 12 c2jmrA_ 13 d2uy6b1 14 c2w07B_ 15 d1n12a_ 16 c2wmpB_ 17 c3cl5A_ 18 c3nqnB_ 19 d3c7bb3 20 d2akja4 21 22 23 24

Detailed template information

# Template Alignment Coverage 3D Model Confidence % i.d. Template Information

1 c1klfP_ 99.8 21 PDB header:chaperone/adhesin complex Chain: P: PDB Molecule:fimh protein; PDBTitle: fimh adhesin-fimc chaperone complex with d-mannose 2 c2jtyA_ 99.8 25 PDB header:structural protein Chain: A: PDB Molecule:type-1 fimbrial protein, a chain; PDBTitle: self-complemented variant of fima, the main subunit of type 1 pilus 3 d1pdkb_ 99.8 20 Fold:Common fold of diphtheria toxin/transcription factors/cytochrome f Superfamily:Bacterial adhesins Family:Pilus subunits 4 c3jwnK_ 99.8 21 PDB header:protein binding/cell adhesion Chain: K: PDB Molecule:protein fimf; PDBTitle: complex of fimc, fimf, fimg and fimh 5 c3jwnL_ 99.8 21 PDB header:protein binding/cell adhesion Chain: L: PDB Molecule:protein fimf; PDBTitle: complex of fimc, fimf, fimg and fimh 6 c3jwnE_ 99.8 21 PDB header:protein binding/cell adhesion Chain: E: PDB Molecule:protein fimf; PDBTitle: complex of fimc, fimf, fimg and fimh 7 c3jwnF_ 99.8 21 PDB header:protein binding/cell adhesion Chain: F: PDB Molecule:protein fimf; PDBTitle: complex of fimc, fimf, fimg and fimh 8 c3bfwA_ 99.8 17 PDB header:structural protein/structural protein Chain: A: PDB Molecule:protein fimg; PDBTitle: crystal structure of truncated fimg (fimgt) in complex with the donor2 strand peptide of fimf (dsf) 9 c3bwuF_ 99.8 25 PDB header:chaperone, structural, membrane protein Chain: F: PDB Molecule:protein fimf; PDBTitle: crystal structure of the ternary complex of fimd (n-terminal domain,2 fimdn) with fimc and the n-terminally truncated pilus subunit fimf3 (fimft) 10 d2j2zb1 99.8 18 Fold:Common fold of diphtheria toxin/transcription factors/cytochrome f Superfamily:Bacterial adhesins Family:Pilus subunits 11 d1ze3h1 99.7 18 Fold:Common fold of diphtheria toxin/transcription factors/cytochrome f Superfamily:Bacterial adhesins Family:Pilus subunits 12 c2jmrA_ 99.7 22 PDB header:cell adhesion Chain: A: PDB Molecule:fimf; PDBTitle: nmr structure of the e. coli type 1 pilus subunit fimf 13 d2uy6b1 99.7 21 Fold:Common fold of diphtheria toxin/transcription factors/cytochrome f Superfamily:Bacterial adhesins Family:Pilus subunits 14 c2w07B_ 99.7 20 PDB header:cell adhesion Chain: B: PDB Molecule:minor pilin subunit papf; PDBTitle: structural determinants of polymerization reactivity of the2 p pilus adaptor subunit papf 15 d1n12a_ 99.3 16 Fold:Common fold of diphtheria toxin/transcription factors/cytochrome f Superfamily:Bacterial adhesins Family:Pilus subunits 16 c2wmpB_ 51.6 17 PDB header:chaperone Chain: B: PDB Molecule:papg protein; PDBTitle: structure of the e. coli chaperone papd in complex with the pilin2 domain of the papgii adhesin 17 c3cl5A_ 15.2 26 PDB header:hydrolase Chain: A: PDB Molecule:hemagglutinin-esterase; PDBTitle: structure of coronavirus hemagglutinin-esterase in complex with 4,9-o-2 diacetyl sialic acid 18 c3nqnB_ 7.6 13 PDB header:structural genomics, unknown function Chain: B: PDB Molecule:uncharacterized protein; PDBTitle: crystal structure of a protein with unknown function. (dr_2006) from2 deinococcus radiodurans at 1.88 a resolution 19 d3c7bb3 7.3 21 Fold:Nitrite and sulphite reductase 4Fe-4S domain-like Superfamily:Nitrite and sulphite reductase 4Fe-4S domain-like Family:Nitrite and sulphite reductase 4Fe-4S domain-like 20 d2akja4 6.6 7 Fold:Nitrite and sulphite reductase 4Fe-4S domain-like Superfamily:Nitrite and sulphite reductase 4Fe-4S domain-like Family:Nitrite and sulphite reductase 4Fe-4S domain-like 21 c2i9lL_ not modelled 6.5 21 PDB header:immune system/viral protein Chain: L: PDB Molecule:virion membrane protein m25; PDBTitle: structure of fab 7d11 from a neutralizing antibody against the2 poxvirus l1 protein 22 d2v4jb3 not modelled 5.9 54 Fold:Nitrite and sulphite reductase 4Fe-4S domain-like Superfamily:Nitrite and sulphite reductase 4Fe-4S domain-like Family:Nitrite and sulphite reductase 4Fe-4S domain-like 23 d3c7ba3 not modelled 5.7 31 Fold:Nitrite and sulphite reductase 4Fe-4S domain-like Superfamily:Nitrite and sulphite reductase 4Fe-4S domain-like Family:Nitrite and sulphite reductase 4Fe-4S domain-like 24 d1lvla1 not modelled 5.3 22 Fold:FAD/NAD(P)-binding domain Superfamily:FAD/NAD(P)-binding domain Family:FAD/NAD-linked reductases, N-terminal and central domains

Binding site prediction

Due to computational demand, binding site predictions are not run for batch jobs

If you want to predict binding sites, please manually submit your model of choice to 3DLigandSite

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Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
If you use the binding site predictions from 3DLigandSite, please also cite:
3DLigandSite: predicting ligand-binding sites using similar structures.
Wass MN, Kelley LA and Sternberg MJ Nucleic Acids Research 38, W469-73 (2010) [PubMed]
© Structural Bioinformatics Group Imperial College London Lawrence Kelley, Benjamin Jefferys Disclaimer Terms and Conditions	Component software Template detection: HHpred 1.51 Secondary structure prediction: Psi-pred 2.5 Disorder prediction: Disopred 2.4 Transmembrane prediction: Memsat_SVM Multi-template modelling and ab initio: Poing 1.0