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Job DescriptionP76108
Confidence100.00%DateThu Jan 5 12:19:01 GMT 2012
Rank1Aligned Residues336
% Identity15%Templatec3osqA_
PDB info PDB header:fluorescent protein, transport proteinChain: A: PDB Molecule:maltose-binding periplasmic protein, green fluorescent PDBTitle: maltose-bound maltose sensor engineered by insertion of circularly2 permuted green fluorescent protein into e. coli maltose binding3 protein at position 175
Resolution1.90 Å

  Insertion relative to template
  Deletion relative to template
  Catalytic residue from the CSA
 
Detailed help on interpreting your alignment


   37..40.........50.........60.........70....... ..80.........90.........100.........110....
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Query Sequence  DIIAWPGYIERGQTDKQYDWVTQFEKETGCAVNVKTAATSD. . EMVSLMTKGGYDLVTASGDASLRLIMGKRVQPINTAL
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Template Sequence  KLVIWIN. . GDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITP. D
Template Known Secondary structure  S

..TTS





TTTT
S
SGGGTT
B




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   6...10.. .......20.........30.........40.........50.........60.........70.........80. .
 
   115....120.........130.........140.........150.........160.........170.........180.........190....
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Query Sequence  IPNWKTLDPRVVKGDWFNVGGKVYGTPYQWGPNLLMYNTKTFPTPPDSWQVVFVEQNLPDGKSNKGRVQAYDGPIYIADA
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Template Sequence  KAFQDKLYPFTWDA. . VRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPL
Template Known Secondary structure  TTB
..TTT
S

TTT
SS

SBSTTTTT




SS
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   83......90...... ...100.........110.........120.........130.........140.........150.........160
 
   195....200
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Query Sequence  ALFVKA. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
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Template Sequence  IAADGGYAFKYENHLSHNVYIMADKQKNGIKANFKIRHNIEDGGVQLAYHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDP
Template Known Secondary structure  TT

S


GGGTBTTS
SSSS





S

T
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   161........170.........180.........190.........200.........210.........220.........230.........240
 
  
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Query Sequence  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Query Conservation  ................................................................................
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Template Sequence  NEKRDHMVLLEFVTAAGITLGMDELYKGVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGK
Template Known Secondary structure  T

SS




TT


TGGGGGSSTTTTTTTTSS
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   241........250.........260.........270.........280.........290.........300.........310.........320
 
  
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Query Sequence  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
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Template Sequence  LPVPWPTLVTTLVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGN
Template Known Secondary structure 
SS
GGGGTTT

GGG
B

GGGGGG
TTTT
TTS
TTS

TTST
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   327..330.........340.........350.........360.........370.........380.........390.........400......
 
   201........210.........220.........230....... ..240.........250.........260.........270
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Query Sequence  . . . . . . . . . TQPQLGISDPYQLTEEQYQAVLKVLRAQHSLIHRYWH. DTTVQMSDFKNEGVVASSAWPYQANALKAEGQP
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Template Sequence  ILGHKLEYNFNGGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVN
Template Known Secondary structure  TTTT
B
SSSTTTTSSSTTSS
TT

TTS
GGGT

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   410.........420.........430.........440.........450.........460.........470.........480.........
 
   271........ 280.........290.........300.........310.........320.........330.........340....
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Query Sequence  VATVFPKEG. . . . . . VTGWADTTMLHSEAKHPVCAYKWMNWSLTPKVQGDVAAWFGSLPVVPEGCKASPLLGEKGCETNG
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Template Sequence  YGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYEEELVKDPRIAA. .
Template Known Secondary structure 


BBTTB



BTT
S
TTSSS


SBTTS..
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   490.........500.........510.........520.........530.........540.........550.........560.......
 
   345....350.........360.........370.........380.
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Query Sequence  FNYFDKIAFWKTPIAEGGKFVPYSRWTQDYIAIMGGR
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Template Sequence  . . TMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGR
Template Known Secondary structure  ..S



TTTTS
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   568.570.........580.........590.........600..
 
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Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
 
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Lawrence Kelley, Benjamin Jefferys 
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