Secondary structure and disorder prediction

		1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	M	A	R	R	S	N	A	V	R	P	L	T	I	I	S	H	Q	H	Q	T	G	G	I	N	I	Q	P	P	R	R	M	Q	F	P	R	Y	R	F	I	K	K	I	E	H	R	W	V	I	R	V	V	R	G	A	N	I	A	L	R
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?								?	?					?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		L	I	E	H	E	V	T	W	T	V	L	L	R	Q	R	V	A	I	V	S	D	I	M	F	R	K	Q	F	E	R	C	I	T	D	D	F	A	I	D	G	D	T	I	A	A	D	F	T	P	G	N	S	T	A	N	A	E	L	L	C
Secondary structure
SS confidence
Disorder																																													?				?	?	?		?				?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.
Sequence		D	K	F	I	K	S	H	V	C	D	F	A	C	K	N	G	G	R	A	L	T	R	K	S	E	L	L	S	A	Q	Y	S	G	L	I	T	S	L	K	G	K	R	S	M	A	Y	Y
Secondary structure
SS confidence
Disorder																?	?	?	?	?	?	?	?																?			?	?	?	?	?	?	?
Disorder confidence

Confidence Key
High(9)											Low (0)

?	Disordered
	Alpha helix
	Beta strand

Domain analysis

Hover over an aligned region to see model and summary info

Please note, only up to the top 20 hits are modelled to reduce computer load

Rank Aligned region

PDB 3jru chain B Region: 21 - 37 Aligned: 17 Modelled: 17 Confidence: 30.5% Identity: 29% PDB header:hydrolase Chain: B: PDB Molecule:probable cytosol aminopeptidase; PDBTitle: crystal structure of leucyl aminopeptidase (pepa) from xoo0834,2 xanthomonas oryzae pv. oryzae kacc10331 Phyre2 PDB 3ij3 chain A Region: 5 - 37 Aligned: 33 Modelled: 33 Confidence: 29.6% Identity: 18% PDB header:hydrolase Chain: A: PDB Molecule:cytosol aminopeptidase; PDBTitle: 1.8 angstrom resolution crystal structure of cytosol aminopeptidase2 from coxiella burnetii Phyre2 PDB 3h8g chain C Region: 5 - 37 Aligned: 33 Modelled: 33 Confidence: 28.4% Identity: 27% PDB header:hydrolase Chain: C: PDB Molecule:cytosol aminopeptidase; PDBTitle: bestatin complex structure of leucine aminopeptidase from pseudomonas2 putida Phyre2 PDB 1lan chain A Region: 10 - 37 Aligned: 28 Modelled: 28 Confidence: 28.1% Identity: 29% PDB header:hydrolase (alpha-aminoacylpeptide) Chain: A: PDB Molecule:leucine aminopeptidase; PDBTitle: leucine aminopeptidase complex with l-leucinal Phyre2 PDB 1v9v chain A domain 1 Region: 29 - 66 Aligned: 38 Modelled: 38 Confidence: 28.0% Identity: 16% Fold: Bromodomain-like Superfamily: MAST3 pre-PK domain-like Family: MAST3 pre-PK domain-like Phyre2 PDB 1lam chain A domain 1 Region: 21 - 37 Aligned: 17 Modelled: 17 Confidence: 25.9% Identity: 35% Fold: Phosphorylase/hydrolase-like Superfamily: Zn-dependent exopeptidases Family: Leucine aminopeptidase, C-terminal domain Phyre2 PDB 3bj4 chain B Region: 59 - 86 Aligned: 25 Modelled: 28 Confidence: 22.1% Identity: 36% PDB header:signaling protein Chain: B: PDB Molecule:potassium voltage-gated channel subfamily kqt PDBTitle: the kcnq1 (kv7.1) c-terminus, a multi-tiered scaffold for2 subunit assembly and protein interaction Phyre2 PDB 1gyt chain A domain 2 Region: 21 - 37 Aligned: 17 Modelled: 17 Confidence: 20.0% Identity: 41% Fold: Phosphorylase/hydrolase-like Superfamily: Zn-dependent exopeptidases Family: Leucine aminopeptidase, C-terminal domain Phyre2 PDB 2hc9 chain A Region: 21 - 37 Aligned: 17 Modelled: 17 Confidence: 18.1% Identity: 29% PDB header:hydrolase Chain: A: PDB Molecule:leucine aminopeptidase 1; PDBTitle: structure of caenorhabditis elegans leucine aminopeptidase-zinc2 complex (lap1) Phyre2 PDB 1gyt chain G Region: 21 - 37 Aligned: 17 Modelled: 17 Confidence: 17.7% Identity: 41% PDB header:hydrolase Chain: G: PDB Molecule:cytosol aminopeptidase; PDBTitle: e. coli aminopeptidase a (pepa) Phyre2 PDB 3hfe chain C Region: 59 - 81 Aligned: 20 Modelled: 23 Confidence: 16.9% Identity: 45% PDB header:transport protein Chain: C: PDB Molecule:potassium voltage-gated channel subfamily kqt member 1; PDBTitle: a trimeric form of the kv7.1 a domain tail Phyre2 PDB 3h7h chain A Region: 122 - 163 Aligned: 42 Modelled: 42 Confidence: 13.4% Identity: 19% PDB header:transcription Chain: A: PDB Molecule:transcription elongation factor spt4; PDBTitle: crystal structure of the human transcription elongation factor dsif,2 hspt4/hspt5 (176-273) Phyre2 PDB 3kzw chain D Region: 21 - 37 Aligned: 17 Modelled: 17 Confidence: 13.0% Identity: 24% PDB header:hydrolase Chain: D: PDB Molecule:cytosol aminopeptidase; PDBTitle: crystal structure of cytosol aminopeptidase from staphylococcus aureus2 col Phyre2 PDB 3iwc chain D Region: 3 - 24 Aligned: 21 Modelled: 22 Confidence: 12.8% Identity: 29% PDB header:lyase Chain: D: PDB Molecule:s-adenosylmethionine decarboxylase; PDBTitle: t. maritima adometdc complex with s-adenosylmethionine2 methyl ester Phyre2 PDB 2obv chain A Region: 75 - 137 Aligned: 62 Modelled: 63 Confidence: 12.6% Identity: 16% PDB header:transferase Chain: A: PDB Molecule:s-adenosylmethionine synthetase isoform type-1; PDBTitle: crystal structure of the human s-adenosylmethionine synthetase 1 in2 complex with the product Phyre2 PDB 3kr5 chain E Region: 21 - 37 Aligned: 17 Modelled: 17 Confidence: 12.5% Identity: 24% PDB header:hydrolase Chain: E: PDB Molecule:m17 leucyl aminopeptidase; PDBTitle: structure of a protease 4 Phyre2 PDB 2kzt chain A Region: 116 - 153 Aligned: 38 Modelled: 38 Confidence: 12.1% Identity: 29% PDB header:apoptosis Chain: A: PDB Molecule:programmed cell death protein 4; PDBTitle: structure of the tandem ma-3 region of pdcd4 Phyre2 PDB 2d8x chain A domain 1 Region: 133 - 143 Aligned: 11 Modelled: 11 Confidence: 10.4% Identity: 55% Fold: Glucocorticoid receptor-like (DNA-binding domain) Superfamily: Glucocorticoid receptor-like (DNA-binding domain) Family: LIM domain Phyre2 PDB 3iml chain B Region: 71 - 137 Aligned: 66 Modelled: 67 Confidence: 9.9% Identity: 20% PDB header:transferase Chain: B: PDB Molecule:s-adenosylmethionine synthetase; PDBTitle: crystal structure of s-adenosylmethionine synthetase from burkholderia2 pseudomallei Phyre2 PDB 1sr9 chain A domain 1 Region: 25 - 44 Aligned: 20 Modelled: 20 Confidence: 8.5% Identity: 40% Fold: RuvA C-terminal domain-like Superfamily: post-HMGL domain-like Family: DmpG/LeuA communication domain-like Phyre2 1 c3jruB_ 2 c3ij3A_ 3 c3h8gC_ 4 c1lanA_ 5 d1v9va1 6 d1lama1 7 c3bj4B_ 8 d1gyta2 9 c2hc9A_ 10 c1gytG_ 11 c3hfeC_ 12 c3h7hA_ 13 c3kzwD_ 14 c3iwcD_ 15 c2obvA_ 16 c3kr5E_ 17 c2kztA_ 18 d2d8xa1 19 c3imlB_ 20 d1sr9a1 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40

Detailed template information

# Template Alignment Coverage 3D Model Confidence % i.d. Template Information

1 c3jruB_ 30.5 29 PDB header:hydrolase Chain: B: PDB Molecule:probable cytosol aminopeptidase; PDBTitle: crystal structure of leucyl aminopeptidase (pepa) from xoo0834,2 xanthomonas oryzae pv. oryzae kacc10331 2 c3ij3A_ 29.6 18 PDB header:hydrolase Chain: A: PDB Molecule:cytosol aminopeptidase; PDBTitle: 1.8 angstrom resolution crystal structure of cytosol aminopeptidase2 from coxiella burnetii 3 c3h8gC_ 28.4 27 PDB header:hydrolase Chain: C: PDB Molecule:cytosol aminopeptidase; PDBTitle: bestatin complex structure of leucine aminopeptidase from pseudomonas2 putida 4 c1lanA_ 28.1 29 PDB header:hydrolase (alpha-aminoacylpeptide) Chain: A: PDB Molecule:leucine aminopeptidase; PDBTitle: leucine aminopeptidase complex with l-leucinal 5 d1v9va1 28.0 16 Fold:Bromodomain-like Superfamily:MAST3 pre-PK domain-like Family:MAST3 pre-PK domain-like 6 d1lama1 25.9 35 Fold:Phosphorylase/hydrolase-like Superfamily:Zn-dependent exopeptidases Family:Leucine aminopeptidase, C-terminal domain 7 c3bj4B_ 22.1 36 PDB header:signaling protein Chain: B: PDB Molecule:potassium voltage-gated channel subfamily kqt PDBTitle: the kcnq1 (kv7.1) c-terminus, a multi-tiered scaffold for2 subunit assembly and protein interaction 8 d1gyta2 20.0 41 Fold:Phosphorylase/hydrolase-like Superfamily:Zn-dependent exopeptidases Family:Leucine aminopeptidase, C-terminal domain 9 c2hc9A_ 18.1 29 PDB header:hydrolase Chain: A: PDB Molecule:leucine aminopeptidase 1; PDBTitle: structure of caenorhabditis elegans leucine aminopeptidase-zinc2 complex (lap1) 10 c1gytG_ 17.7 41 PDB header:hydrolase Chain: G: PDB Molecule:cytosol aminopeptidase; PDBTitle: e. coli aminopeptidase a (pepa) 11 c3hfeC_ 16.9 45 PDB header:transport protein Chain: C: PDB Molecule:potassium voltage-gated channel subfamily kqt member 1; PDBTitle: a trimeric form of the kv7.1 a domain tail 12 c3h7hA_ 13.4 19 PDB header:transcription Chain: A: PDB Molecule:transcription elongation factor spt4; PDBTitle: crystal structure of the human transcription elongation factor dsif,2 hspt4/hspt5 (176-273) 13 c3kzwD_ 13.0 24 PDB header:hydrolase Chain: D: PDB Molecule:cytosol aminopeptidase; PDBTitle: crystal structure of cytosol aminopeptidase from staphylococcus aureus2 col 14 c3iwcD_ 12.8 29 PDB header:lyase Chain: D: PDB Molecule:s-adenosylmethionine decarboxylase; PDBTitle: t. maritima adometdc complex with s-adenosylmethionine2 methyl ester 15 c2obvA_ 12.6 16 PDB header:transferase Chain: A: PDB Molecule:s-adenosylmethionine synthetase isoform type-1; PDBTitle: crystal structure of the human s-adenosylmethionine synthetase 1 in2 complex with the product 16 c3kr5E_ 12.5 24 PDB header:hydrolase Chain: E: PDB Molecule:m17 leucyl aminopeptidase; PDBTitle: structure of a protease 4 17 c2kztA_ 12.1 29 PDB header:apoptosis Chain: A: PDB Molecule:programmed cell death protein 4; PDBTitle: structure of the tandem ma-3 region of pdcd4 18 d2d8xa1 10.4 55 Fold:Glucocorticoid receptor-like (DNA-binding domain) Superfamily:Glucocorticoid receptor-like (DNA-binding domain) Family:LIM domain 19 c3imlB_ 9.9 20 PDB header:transferase Chain: B: PDB Molecule:s-adenosylmethionine synthetase; PDBTitle: crystal structure of s-adenosylmethionine synthetase from burkholderia2 pseudomallei 20 d1sr9a1 8.5 40 Fold:RuvA C-terminal domain-like Superfamily:post-HMGL domain-like Family:DmpG/LeuA communication domain-like 21 c1mtpB_ not modelled 8.5 33 PDB header:structural genomics Chain: B: PDB Molecule:serine proteinase inhibitor (serpin), chain b; PDBTitle: the x-ray crystal structure of a serpin from a thermophilic2 prokaryote 22 d2q49a2 not modelled 8.2 11 Fold:FwdE/GAPDH domain-like Superfamily:Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain Family:GAPDH-like 23 d1edla_ not modelled 8.1 44 Fold:immunoglobulin/albumin-binding domain-like Superfamily:Bacterial immunoglobulin/albumin-binding domains Family:Immunoglobulin-binding protein A modules 24 c2wshC_ not modelled 7.8 23 PDB header:hydrolase Chain: C: PDB Molecule:endonuclease ii; PDBTitle: structure of bacteriophage t4 endoii e118a mutant 25 d1uc2a_ not modelled 7.8 16 Fold:Hypothetical protein PH1602 Superfamily:Hypothetical protein PH1602 Family:Hypothetical protein PH1602 26 c2eloA_ not modelled 7.2 71 PDB header:transcription Chain: A: PDB Molecule:zinc finger protein 406; PDBTitle: solution structure of the 12th c2h2 zinc finger of human2 zinc finger protein 406 27 d1vjpa1 not modelled 7.0 9 Fold:NAD(P)-binding Rossmann-fold domains Superfamily:NAD(P)-binding Rossmann-fold domains Family:Glyceraldehyde-3-phosphate dehydrogenase-like, N-terminal domain 28 d1fc2c_ not modelled 6.7 41 Fold:immunoglobulin/albumin-binding domain-like Superfamily:Bacterial immunoglobulin/albumin-binding domains Family:Immunoglobulin-binding protein A modules 29 d1ryqa_ not modelled 6.7 28 Fold:Rubredoxin-like Superfamily:RNA polymerase subunits Family:RpoE2-like 30 d2db7a1 not modelled 6.5 18 Fold:Orange domain-like Superfamily:Orange domain-like Family:Hairy Orange domain 31 c2zbtB_ not modelled 6.4 33 PDB header:lyase Chain: B: PDB Molecule:pyridoxal biosynthesis lyase pdxs; PDBTitle: crystal structure of pyridoxine biosynthesis protein from thermus2 thermophilus hb8 32 c3so4C_ not modelled 6.3 16 PDB header:transferase Chain: C: PDB Molecule:methionine-adenosyltransferase; PDBTitle: methionine-adenosyltransferase from entamoeba histolytica 33 c3femB_ not modelled 6.3 40 PDB header:biosynthetic protein, transferase Chain: B: PDB Molecule:pyridoxine biosynthesis protein snz1; PDBTitle: structure of the synthase subunit pdx1.1 (snz1) of plp synthase from2 saccharomyces cerevisiae 34 d2p02a2 not modelled 6.1 47 Fold:S-adenosylmethionine synthetase Superfamily:S-adenosylmethionine synthetase Family:S-adenosylmethionine synthetase 35 d1prtc2 not modelled 6.1 78 Fold:C-type lectin-like Superfamily:C-type lectin-like Family:Aerolysin/Pertussis toxin (APT) domain 36 c2epgB_ not modelled 6.1 18 PDB header:structural genomics, unknown function Chain: B: PDB Molecule:hypothetical protein ttha1785; PDBTitle: crystal structure of ttha1785 37 c2nv2U_ not modelled 5.9 33 PDB header:lyase/transferase Chain: U: PDB Molecule:pyridoxal biosynthesis lyase pdxs; PDBTitle: structure of the plp synthase complex pdx1/2 (yaad/e) from bacillus2 subtilis 38 c3mtvA_ not modelled 5.8 25 PDB header:hydrolase Chain: A: PDB Molecule:papain-like cysteine protease; PDBTitle: the crystal structure of the prrsv nonstructural protein nsp1 39 d1vkna2 not modelled 5.8 24 Fold:FwdE/GAPDH domain-like Superfamily:Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain Family:GAPDH-like 40 c3rv2B_ not modelled 5.8 16 PDB header:transferase Chain: B: PDB Molecule:s-adenosylmethionine synthase; PDBTitle: crystal structure of s-adenosylmethionine synthetase from2 mycobacterium marinum

Binding site prediction

Due to computational demand, binding site predictions are not run for batch jobs

If you want to predict binding sites, please manually submit your model of choice to 3DLigandSite

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Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
If you use the binding site predictions from 3DLigandSite, please also cite:
3DLigandSite: predicting ligand-binding sites using similar structures.
Wass MN, Kelley LA and Sternberg MJ Nucleic Acids Research 38, W469-73 (2010) [PubMed]
© Structural Bioinformatics Group Imperial College London Lawrence Kelley, Benjamin Jefferys Disclaimer Terms and Conditions	Component software Template detection: HHpred 1.51 Secondary structure prediction: Psi-pred 2.5 Disorder prediction: Disopred 2.4 Transmembrane prediction: Memsat_SVM Multi-template modelling and ab initio: Poing 1.0