Secondary structure and disorder prediction

		1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	K	T	E	R	I	L	G	A	L	Y	G	Q	A	L	G	D	A	M	G	M	P	S	E	L	W	P	R	S	R	V	K	A	H	F	G	W	I	D	R	F	L	P	G	P	K	E	N	N	A	A	C	Y	F	N	R	A	E	F	T
Secondary structure
SS confidence
Disorder		?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		D	D	T	S	M	A	L	C	L	A	D	A	L	L	E	R	E	G	K	I	D	P	D	L	I	G	R	N	I	L	D	W	A	L	R	F	D	A	F	N	K	N	V	L	G	P	T	S	K	I	A	L	N	A	I	R	D	G	K	P
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		V	A	E	L	E	N	N	G	V	T	N	G	A	A	M	R	V	S	P	L	G	C	L	L	P	A	R	D	V	D	S	F	I	D	D	V	A	L	A	S	S	P	T	H	K	S	D	L	A	V	A	G	A	V	V	I	A	W	A	I
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		S	R	A	I	D	G	E	S	W	S	A	I	V	D	S	L	P	S	I	A	R	H	A	Q	Q	K	R	I	T	T	F	S	A	S	L	A	A	R	L	E	I	A	L	K	I	V	R	N	A	D	G	T	E	S	A	S	E	Q	L	Y
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300
Sequence		Q	V	V	G	A	G	T	S	T	I	E	S	V	P	C	A	I	A	L	V	E	L	A	Q	T	D	P	N	R	C	A	V	L	C	A	N	L	G	G	D	T	D	T	I	G	A	M	A	T	A	I	C	G	A	L	H	G	V	N	A
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	310	.	.	.	.	.	.	.	.	.	320	.	.	.	.	.	.	.	.	.	330	.	.	.	.
Sequence		I	D	P	A	L	K	A	E	L	D	A	V	N	Q	L	D	F	N	R	Y	A	T	A	L	A	K	Y	R	Q	Q	R	E	A	V
Secondary structure
SS confidence
Disorder																																?	?	?	?
Disorder confidence

Confidence Key
High(9)											Low (0)

?	Disordered
	Alpha helix
	Beta strand

Domain analysis

Hover over an aligned region to see model and summary info

Please note, only up to the top 20 hits are modelled to reduce computer load

Rank Aligned region

PDB 3g9d chain B Region: 3 - 326 Aligned: 282 Modelled: 289 Confidence: 100.0% Identity: 23% PDB header:hydrolase Chain: B: PDB Molecule:dinitrogenase reductase activacting PDBTitle: crystal structure glycohydrolase Phyre2 PDB 2woc chain A Region: 3 - 328 Aligned: 284 Modelled: 284 Confidence: 100.0% Identity: 23% PDB header:hydrolase Chain: A: PDB Molecule:adp-ribosyl-[dinitrogen reductase] glycohydrolase; PDBTitle: crystal structure of the dinitrogenase reductase-activating2 glycohydrolase (drag) from rhodospirillum rubrum Phyre2 PDB 1t5j chain A Region: 3 - 330 Aligned: 297 Modelled: 312 Confidence: 100.0% Identity: 29% Fold: ADP-ribosylglycohydrolase Superfamily: ADP-ribosylglycohydrolase Family: ADP-ribosylglycohydrolase Phyre2 PDB 3hfw chain A Region: 4 - 334 Aligned: 321 Modelled: 331 Confidence: 100.0% Identity: 20% PDB header:hydrolase Chain: A: PDB Molecule:protein adp-ribosylarginine hydrolase; PDBTitle: crystal structure of human adp-ribosylhydrolase 1 (harh1) Phyre2 PDB 2qty chain B Region: 4 - 330 Aligned: 308 Modelled: 325 Confidence: 100.0% Identity: 25% PDB header:hydrolase Chain: B: PDB Molecule:poly(adp-ribose) glycohydrolase arh3; PDBTitle: crystal structure of mouse adp-ribosylhydrolase 3 (marh3) Phyre2 PDB 2yzw chain A Region: 2 - 328 Aligned: 279 Modelled: 304 Confidence: 100.0% Identity: 26% PDB header:hydrolase Chain: A: PDB Molecule:adp-ribosylglycohydrolase; PDBTitle: adp-ribosylglycohydrolase-related protein complex Phyre2 PDB 2i7n chain A domain 2 Region: 246 - 304 Aligned: 57 Modelled: 59 Confidence: 19.1% Identity: 14% Fold: Ribonuclease H-like motif Superfamily: Actin-like ATPase domain Family: Fumble-like Phyre2 PDB 1j9j chain A Region: 276 - 330 Aligned: 51 Modelled: 55 Confidence: 16.8% Identity: 24% Fold: SurE-like Superfamily: SurE-like Family: SurE-like Phyre2 PDB 2odb chain B Region: 297 - 310 Aligned: 14 Modelled: 14 Confidence: 15.8% Identity: 29% PDB header:protein binding Chain: B: PDB Molecule:serine/threonine-protein kinase pak 6; PDBTitle: the crystal structure of human cdc42 in complex with the crib domain2 of human p21-activated kinase 6 (pak6) Phyre2 PDB 1mw7 chain A Region: 267 - 308 Aligned: 40 Modelled: 42 Confidence: 14.3% Identity: 25% Fold: YebC-like Superfamily: YebC-like Family: YebC-like Phyre2 PDB 1e0a chain B Region: 297 - 314 Aligned: 18 Modelled: 18 Confidence: 13.1% Identity: 17% PDB header:signalling protein Chain: B: PDB Molecule:serine/threonine-protein kinase pak-alpha; PDBTitle: cdc42 complexed with the gtpase binding domain of p212 activated kinase Phyre2 PDB 3smp chain A Region: 246 - 304 Aligned: 57 Modelled: 59 Confidence: 11.8% Identity: 14% PDB header:transferase Chain: A: PDB Molecule:pantothenate kinase 1; PDBTitle: monoclinic crystal structure of human pantothenate kinase 1 alpha Phyre2 PDB 2i7p chain A domain 1 Region: 246 - 304 Aligned: 57 Modelled: 59 Confidence: 11.4% Identity: 12% Fold: Ribonuclease H-like motif Superfamily: Actin-like ATPase domain Family: Fumble-like Phyre2 PDB 1f3m chain B Region: 297 - 314 Aligned: 18 Modelled: 18 Confidence: 10.1% Identity: 17% PDB header:transferase Chain: B: PDB Molecule:serine/threonine-protein kinase pak-alpha; PDBTitle: crystal structure of human serine/threonine kinase pak1 Phyre2 PDB 1cee chain B Region: 297 - 316 Aligned: 20 Modelled: 20 Confidence: 9.5% Identity: 30% PDB header:structural protein regulation Chain: B: PDB Molecule:wiskott-aldrich syndrome protein wasp; PDBTitle: solution structure of cdc42 in complex with the gtpase2 binding domain of wasp Phyre2 PDB 2phj chain A Region: 276 - 301 Aligned: 26 Modelled: 26 Confidence: 9.2% Identity: 35% PDB header:hydrolase Chain: A: PDB Molecule:5'-nucleotidase sure; PDBTitle: crystal structure of sure protein from aquifex aeolicus Phyre2 PDB 2i7p chain A Region: 246 - 304 Aligned: 57 Modelled: 59 Confidence: 8.6% Identity: 12% PDB header:transferase Chain: A: PDB Molecule:pantothenate kinase 3; PDBTitle: crystal structure of human pank3 in complex with accoa Phyre2 PDB 2j0w chain A Region: 279 - 294 Aligned: 16 Modelled: 16 Confidence: 8.3% Identity: 25% PDB header:transferase Chain: A: PDB Molecule:lysine-sensitive aspartokinase 3; PDBTitle: crystal structure of e. coli aspartokinase iii in complex2 with aspartate and adp (r-state) Phyre2 PDB 2e6g chain I Region: 276 - 301 Aligned: 26 Modelled: 26 Confidence: 8.3% Identity: 38% PDB header:hydrolase Chain: I: PDB Molecule:5'-nucleotidase sure; PDBTitle: crystal structure of the stationary phase survival protein sure from2 thermus thermophilus hb8 in complex with phosphate Phyre2 PDB 3c9p chain A Region: 254 - 326 Aligned: 63 Modelled: 68 Confidence: 8.2% Identity: 17% PDB header:structural genomics, unknown function Chain: A: PDB Molecule:uncharacterized protein sp1917; PDBTitle: crystal structure of uncharacterized protein sp1917 Phyre2 1 c3g9dB_ 2 c2wocA_ 3 d1t5ja_ 4 c3hfwA_ 5 c2qtyB_ 6 c2yzwA_ 7 d2i7na2 8 d1j9ja_ 9 c2odbB_ 10 d1mw7a_ 11 c1e0aB_ 12 c3smpA_ 13 d2i7pa1 14 c1f3mB_ 15 c1ceeB_ 16 c2phjA_ 17 c2i7pA_ 18 c2j0wA_ 19 c2e6gI_ 20 c3c9pA_ 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38

Detailed template information

# Template Alignment Coverage 3D Model Confidence % i.d. Template Information

1 c3g9dB_ 100.0 23 PDB header:hydrolase Chain: B: PDB Molecule:dinitrogenase reductase activacting PDBTitle: crystal structure glycohydrolase 2 c2wocA_ 100.0 23 PDB header:hydrolase Chain: A: PDB Molecule:adp-ribosyl-[dinitrogen reductase] glycohydrolase; PDBTitle: crystal structure of the dinitrogenase reductase-activating2 glycohydrolase (drag) from rhodospirillum rubrum 3 d1t5ja_ 100.0 29 Fold:ADP-ribosylglycohydrolase Superfamily:ADP-ribosylglycohydrolase Family:ADP-ribosylglycohydrolase 4 c3hfwA_ 100.0 20 PDB header:hydrolase Chain: A: PDB Molecule:protein adp-ribosylarginine hydrolase; PDBTitle: crystal structure of human adp-ribosylhydrolase 1 (harh1) 5 c2qtyB_ 100.0 25 PDB header:hydrolase Chain: B: PDB Molecule:poly(adp-ribose) glycohydrolase arh3; PDBTitle: crystal structure of mouse adp-ribosylhydrolase 3 (marh3) 6 c2yzwA_ 100.0 26 PDB header:hydrolase Chain: A: PDB Molecule:adp-ribosylglycohydrolase; PDBTitle: adp-ribosylglycohydrolase-related protein complex 7 d2i7na2 19.1 14 Fold:Ribonuclease H-like motif Superfamily:Actin-like ATPase domain Family:Fumble-like 8 d1j9ja_ 16.8 24 Fold:SurE-like Superfamily:SurE-like Family:SurE-like 9 c2odbB_ 15.8 29 PDB header:protein binding Chain: B: PDB Molecule:serine/threonine-protein kinase pak 6; PDBTitle: the crystal structure of human cdc42 in complex with the crib domain2 of human p21-activated kinase 6 (pak6) 10 d1mw7a_ 14.3 25 Fold:YebC-like Superfamily:YebC-like Family:YebC-like 11 c1e0aB_ 13.1 17 PDB header:signalling protein Chain: B: PDB Molecule:serine/threonine-protein kinase pak-alpha; PDBTitle: cdc42 complexed with the gtpase binding domain of p212 activated kinase 12 c3smpA_ 11.8 14 PDB header:transferase Chain: A: PDB Molecule:pantothenate kinase 1; PDBTitle: monoclinic crystal structure of human pantothenate kinase 1 alpha 13 d2i7pa1 11.4 12 Fold:Ribonuclease H-like motif Superfamily:Actin-like ATPase domain Family:Fumble-like 14 c1f3mB_ 10.1 17 PDB header:transferase Chain: B: PDB Molecule:serine/threonine-protein kinase pak-alpha; PDBTitle: crystal structure of human serine/threonine kinase pak1 15 c1ceeB_ 9.5 30 PDB header:structural protein regulation Chain: B: PDB Molecule:wiskott-aldrich syndrome protein wasp; PDBTitle: solution structure of cdc42 in complex with the gtpase2 binding domain of wasp 16 c2phjA_ 9.2 35 PDB header:hydrolase Chain: A: PDB Molecule:5'-nucleotidase sure; PDBTitle: crystal structure of sure protein from aquifex aeolicus 17 c2i7pA_ 8.6 12 PDB header:transferase Chain: A: PDB Molecule:pantothenate kinase 3; PDBTitle: crystal structure of human pank3 in complex with accoa 18 c2j0wA_ 8.3 25 PDB header:transferase Chain: A: PDB Molecule:lysine-sensitive aspartokinase 3; PDBTitle: crystal structure of e. coli aspartokinase iii in complex2 with aspartate and adp (r-state) 19 c2e6gI_ 8.3 38 PDB header:hydrolase Chain: I: PDB Molecule:5'-nucleotidase sure; PDBTitle: crystal structure of the stationary phase survival protein sure from2 thermus thermophilus hb8 in complex with phosphate 20 c3c9pA_ 8.2 17 PDB header:structural genomics, unknown function Chain: A: PDB Molecule:uncharacterized protein sp1917; PDBTitle: crystal structure of uncharacterized protein sp1917 21 c1ir6A_ not modelled 8.0 13 PDB header:hydrolase Chain: A: PDB Molecule:exonuclease recj; PDBTitle: crystal structure of exonuclease recj bound to manganese 22 d1ir6a_ not modelled 8.0 13 Fold:DHH phosphoesterases Superfamily:DHH phosphoesterases Family:Exonuclease RecJ 23 c3bowC_ not modelled 7.8 25 PDB header:hydrolase/hydrolase inhibitor Chain: C: PDB Molecule:calpastatin; PDBTitle: structure of m-calpain in complex with calpastatin 24 d2j0wa1 not modelled 7.7 25 Fold:Carbamate kinase-like Superfamily:Carbamate kinase-like Family:PyrH-like 25 c3devB_ not modelled 7.5 9 PDB header:structural genomics, unknown function Chain: B: PDB Molecule:sh1221; PDBTitle: crystal structure of sh1221 protein from staphylococcus haemolyticus,2 northeast structural genomics consortium target shr87 26 c1w6kA_ not modelled 6.8 13 PDB header:isomerase Chain: A: PDB Molecule:lanosterol synthase; PDBTitle: structure of human osc in complex with lanosterol 27 c2ogxB_ not modelled 6.7 18 PDB header:metal binding protein Chain: B: PDB Molecule:molybdenum storage protein subunit beta; PDBTitle: the crystal structure of the molybdenum storage protein from2 azotobacter vinelandii loaded with polyoxotungstates (wsto) 28 d1ykwa1 not modelled 6.6 21 Fold:TIM beta/alpha-barrel Superfamily:RuBisCo, C-terminal domain Family:RuBisCo, large subunit, C-terminal domain 29 c2k42A_ not modelled 5.8 14 PDB header:signaling protein Chain: A: PDB Molecule:wiskott-aldrich syndrome protein; PDBTitle: solution structure of the gtpase binding domain of wasp in2 complex with espfu, an ehec effector 30 c3ab4K_ not modelled 5.7 31 PDB header:transferase Chain: K: PDB Molecule:aspartokinase; PDBTitle: crystal structure of feedback inhibition resistant mutant of aspartate2 kinase from corynebacterium glutamicum in complex with lysine and3 threonine 31 c2ap7A_ not modelled 5.6 43 PDB header:antibiotic Chain: A: PDB Molecule:bombinin h2; PDBTitle: solution structure of bombinin h2 in dpc micelles 32 d2cdqa1 not modelled 5.6 19 Fold:Carbamate kinase-like Superfamily:Carbamate kinase-like Family:PyrH-like 33 c2v4oB_ not modelled 5.6 38 PDB header:hydrolase Chain: B: PDB Molecule:multifunctional protein sur e; PDBTitle: crystal structure of salmonella typhimurium sure at 2.752 angstrom resolution in monoclinic form 34 d1w6ka1 not modelled 5.5 10 Fold:alpha/alpha toroid Superfamily:Terpenoid cyclases/Protein prenyltransferases Family:Terpene synthases 35 d2gpia1 not modelled 5.4 10 Fold:Shew3726-like Superfamily:Shew3726-like Family:Shew3726-like 36 d2hmfa1 not modelled 5.3 25 Fold:Carbamate kinase-like Superfamily:Carbamate kinase-like Family:PyrH-like 37 d1ekqa_ not modelled 5.3 19 Fold:Ribokinase-like Superfamily:Ribokinase-like Family:Thiamin biosynthesis kinases 38 c3ll9A_ not modelled 5.0 6 PDB header:transferase Chain: A: PDB Molecule:isopentenyl phosphate kinase; PDBTitle: x-ray structures of isopentenyl phosphate kinase

Binding site prediction

Due to computational demand, binding site predictions are not run for batch jobs

If you want to predict binding sites, please manually submit your model of choice to 3DLigandSite

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Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
If you use the binding site predictions from 3DLigandSite, please also cite:
3DLigandSite: predicting ligand-binding sites using similar structures.
Wass MN, Kelley LA and Sternberg MJ Nucleic Acids Research 38, W469-73 (2010) [PubMed]
© Structural Bioinformatics Group Imperial College London Lawrence Kelley, Benjamin Jefferys Disclaimer Terms and Conditions	Component software Template detection: HHpred 1.51 Secondary structure prediction: Psi-pred 2.5 Disorder prediction: Disopred 2.4 Transmembrane prediction: Memsat_SVM Multi-template modelling and ab initio: Poing 1.0