Secondary structure and disorder prediction

		1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	S	Q	N	K	A	F	S	T	P	F	I	L	A	V	L	C	I	Y	F	S	Y	F	L	H	G	I	S	V	I	T	L	A	Q	N	M	S	S	L	A	E	K	F	S	T	D	N	A	G	I	A	Y	L	I	S	G	I	G	L	G
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?		?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		R	L	I	S	I	L	F	F	G	V	I	S	D	K	F	G	R	R	A	V	I	L	M	A	V	I	M	Y	L	L	F	F	F	G	I	P	A	C	P	N	L	T	L	A	Y	G	L	A	V	C	V	G	I	A	N	S	A	L	D	T
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		G	G	Y	P	A	L	M	E	C	F	P	K	A	S	G	S	A	V	I	L	V	K	A	M	V	S	F	G	Q	M	F	Y	P	M	L	V	S	Y	M	L	L	N	N	I	W	Y	G	Y	G	L	I	I	P	G	I	L	F	V	L	I
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		T	L	M	L	L	K	S	K	F	P	S	Q	L	V	D	A	S	V	T	N	E	L	P	Q	M	N	S	K	P	L	V	W	L	E	G	V	S	S	V	L	F	G	V	A	A	F	S	T	F	Y	V	I	V	V	W	M	P	K	Y	A
Secondary structure
SS confidence
Disorder							?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?		?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300
Sequence		M	A	F	A	G	M	S	E	A	E	A	L	K	T	I	S	Y	Y	S	M	G	S	L	V	C	V	F	I	F	A	A	L	L	K	K	M	V	R	P	I	W	A	N	V	F	N	S	A	L	A	T	I	T	A	A	I	I	Y	L	Y
Secondary structure
SS confidence
Disorder						?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	310	.	.	.	.	.	.	.	.	.	320	.	.	.	.	.	.	.	.	.	330	.	.	.	.	.	.	.	.	.	340	.	.	.	.	.	.	.	.	.	350	.	.	.	.	.	.	.	.	.	360
Sequence		P	S	P	L	V	C	N	A	G	A	F	V	I	G	F	S	A	A	G	G	I	L	Q	L	G	V	S	V	M	S	E	F	F	P	K	S	K	A	K	V	T	S	I	Y	M	M	M	G	G	L	A	N	F	V	I	P	L	I	T	G
Secondary structure
SS confidence
Disorder																																	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	370	.	.	.	.	.	.	.	.	.	380	.	.	.	.	.	.	.	.	.	390	.	.	.	.	.	.	.	.	.	400	.	.	.	.	.	.	.	.	.	410	.	.	.	.	.	.	.	.	.	420
Sequence		Y	L	S	N	I	G	L	Q	Y	I	I	V	L	D	F	T	F	A	L	L	A	L	I	T	A	I	I	V	F	I	R	Y	Y	R	V	F	I	I	P	E	N	D	V	R	F	G	E	R	K	F	C	T	R	L	N	T	I	K	H	R
Secondary structure
SS confidence
Disorder																																			?	?	?	?	?	?	?																			?	?
Disorder confidence
		.
Sequence		G
Secondary structure
SS confidence
Disorder		?
Disorder confidence

Confidence Key
High(9)											Low (0)

?	Disordered
	Alpha helix
	Beta strand

Domain analysis

Hover over an aligned region to see model and summary info

Please note, only up to the top 20 hits are modelled to reduce computer load

Rank Aligned region

PDB 1pw4 chain A Region: 2 - 403 Aligned: 401 Modelled: 402 Confidence: 100.0% Identity: 14% Fold: MFS general substrate transporter Superfamily: MFS general substrate transporter Family: Glycerol-3-phosphate transporter Phyre2 PDB 2gfp chain A Region: 15 - 384 Aligned: 370 Modelled: 370 Confidence: 100.0% Identity: 13% PDB header:membrane protein Chain: A: PDB Molecule:multidrug resistance protein d; PDBTitle: structure of the multidrug transporter emrd from2 escherichia coli Phyre2 PDB 3o7p chain A Region: 9 - 389 Aligned: 373 Modelled: 381 Confidence: 100.0% Identity: 12% PDB header:transport protein Chain: A: PDB Molecule:l-fucose-proton symporter; PDBTitle: crystal structure of the e.coli fucose:proton symporter, fucp (n162a) Phyre2 PDB 1pv7 chain A Region: 13 - 406 Aligned: 393 Modelled: 394 Confidence: 100.0% Identity: 10% Fold: MFS general substrate transporter Superfamily: MFS general substrate transporter Family: LacY-like proton/sugar symporter Phyre2 PDB 2xut chain C Region: 16 - 388 Aligned: 373 Modelled: 373 Confidence: 99.9% Identity: 15% PDB header:transport protein Chain: C: PDB Molecule:proton/peptide symporter family protein; PDBTitle: crystal structure of a proton dependent oligopeptide (pot)2 family transporter. Phyre2 PDB 3b9y chain A Region: 245 - 420 Aligned: 174 Modelled: 176 Confidence: 65.2% Identity: 11% PDB header:transport protein Chain: A: PDB Molecule:ammonium transporter family rh-like protein; PDBTitle: crystal structure of the nitrosomonas europaea rh protein Phyre2 PDB 3rko chain N Region: 7 - 415 Aligned: 396 Modelled: 396 Confidence: 54.5% Identity: 9% PDB header:oxidoreductase Chain: N: PDB Molecule:nadh-quinone oxidoreductase subunit n; PDBTitle: crystal structure of the membrane domain of respiratory complex i from2 e. coli at 3.0 angstrom resolution Phyre2 PDB 3qnq chain D Region: 356 - 407 Aligned: 52 Modelled: 52 Confidence: 29.0% Identity: 21% PDB header:membrane protein, transport protein Chain: D: PDB Molecule:pts system, cellobiose-specific iic component; PDBTitle: crystal structure of the transporter chbc, the iic component from the2 n,n'-diacetylchitobiose-specific phosphotransferase system Phyre2 PDB 2g9p chain A Region: 67 - 80 Aligned: 14 Modelled: 14 Confidence: 16.7% Identity: 50% PDB header:antimicrobial protein Chain: A: PDB Molecule:antimicrobial peptide latarcin 2a; PDBTitle: nmr structure of a novel antimicrobial peptide, latarcin 2a,2 from spider (lachesana tarabaevi) venom Phyre2 PDB 1zxa chain B Region: 394 - 419 Aligned: 26 Modelled: 26 Confidence: 8.9% Identity: 12% PDB header:transferase Chain: B: PDB Molecule:cgmp-dependent protein kinase 1, alpha isozyme; PDBTitle: solution structure of the coiled-coil domain of cgmp-2 dependent protein kinase ia Phyre2 PDB 3hd6 chain A Region: 198 - 418 Aligned: 217 Modelled: 221 Confidence: 8.0% Identity: 14% PDB header:membrane protein, transport protein Chain: A: PDB Molecule:ammonium transporter rh type c; PDBTitle: crystal structure of the human rhesus glycoprotein rhcg Phyre2 PDB 1odr chain A Region: 404 - 421 Aligned: 18 Modelled: 18 Confidence: 7.7% Identity: 22% PDB header:lipid transport Chain: A: PDB Molecule:apoa-i peptide; PDBTitle: peptide of human apoa-i residues 166-185. nmr, 5 structures2 at ph 6.0, 37 degrees celsius and peptide:dpc mole ratio3 of 1:40 Phyre2 PDB 1odq chain A Region: 404 - 421 Aligned: 18 Modelled: 18 Confidence: 7.7% Identity: 22% PDB header:lipid transport Chain: A: PDB Molecule:apoa-i peptide; PDBTitle: peptide of human apoa-i residues 166-185. nmr, 5 structures2 at ph 3.7, 37 degrees celsius and peptide:sds mole ratio3 of 1:40 Phyre2 PDB 1odp chain A Region: 404 - 421 Aligned: 18 Modelled: 18 Confidence: 7.7% Identity: 22% PDB header:lipid transport Chain: A: PDB Molecule:apoa-i peptide; PDBTitle: peptide of human apoa-i residues 166-185. nmr, 5 structures2 at ph 6.6, 37 degrees celsius and peptide:sds mole ratio3 of 1:40 Phyre2 PDB 1vkz chain A domain 1 Region: 399 - 421 Aligned: 23 Modelled: 23 Confidence: 6.0% Identity: 13% Fold: Barrel-sandwich hybrid Superfamily: Rudiment single hybrid motif Family: BC C-terminal domain-like Phyre2 PDB 1rh1 chain A domain 2 Region: 9 - 77 Aligned: 67 Modelled: 69 Confidence: 5.5% Identity: 13% Fold: Toxins' membrane translocation domains Superfamily: Colicin Family: Colicin Phyre2 PDB 2knc chain B Region: 369 - 413 Aligned: 45 Modelled: 45 Confidence: 5.3% Identity: 13% PDB header:cell adhesion Chain: B: PDB Molecule:integrin beta-3; PDBTitle: platelet integrin alfaiib-beta3 transmembrane-cytoplasmic2 heterocomplex Phyre2 1 d1pw4a_ 2 c2gfpA_ 3 c3o7pA_ 4 d1pv7a_ 5 c2xutC_ 6 c3b9yA_ 7 c3rkoN_ 8 c3qnqD_ 9 c2g9pA_ 10 c1zxaB_ 11 c3hd6A_ 12 c1odrA_ 13 c1odqA_ 14 c1odpA_ 15 d1vkza1 16 d1rh1a2 17 c2kncB_

Detailed template information

# Template Alignment Coverage 3D Model Confidence % i.d. Template Information

1 d1pw4a_ 100.0 14 Fold:MFS general substrate transporter Superfamily:MFS general substrate transporter Family:Glycerol-3-phosphate transporter 2 c2gfpA_ 100.0 13 PDB header:membrane protein Chain: A: PDB Molecule:multidrug resistance protein d; PDBTitle: structure of the multidrug transporter emrd from2 escherichia coli 3 c3o7pA_ 100.0 12 PDB header:transport protein Chain: A: PDB Molecule:l-fucose-proton symporter; PDBTitle: crystal structure of the e.coli fucose:proton symporter, fucp (n162a) 4 d1pv7a_ 100.0 10 Fold:MFS general substrate transporter Superfamily:MFS general substrate transporter Family:LacY-like proton/sugar symporter 5 c2xutC_ 99.9 15 PDB header:transport protein Chain: C: PDB Molecule:proton/peptide symporter family protein; PDBTitle: crystal structure of a proton dependent oligopeptide (pot)2 family transporter. 6 c3b9yA_ 65.2 11 PDB header:transport protein Chain: A: PDB Molecule:ammonium transporter family rh-like protein; PDBTitle: crystal structure of the nitrosomonas europaea rh protein 7 c3rkoN_ 54.5 9 PDB header:oxidoreductase Chain: N: PDB Molecule:nadh-quinone oxidoreductase subunit n; PDBTitle: crystal structure of the membrane domain of respiratory complex i from2 e. coli at 3.0 angstrom resolution 8 c3qnqD_ 29.0 21 PDB header:membrane protein, transport protein Chain: D: PDB Molecule:pts system, cellobiose-specific iic component; PDBTitle: crystal structure of the transporter chbc, the iic component from the2 n,n'-diacetylchitobiose-specific phosphotransferase system 9 c2g9pA_ 16.7 50 PDB header:antimicrobial protein Chain: A: PDB Molecule:antimicrobial peptide latarcin 2a; PDBTitle: nmr structure of a novel antimicrobial peptide, latarcin 2a,2 from spider (lachesana tarabaevi) venom 10 c1zxaB_ 8.9 12 PDB header:transferase Chain: B: PDB Molecule:cgmp-dependent protein kinase 1, alpha isozyme; PDBTitle: solution structure of the coiled-coil domain of cgmp-2 dependent protein kinase ia 11 c3hd6A_ 8.0 14 PDB header:membrane protein, transport protein Chain: A: PDB Molecule:ammonium transporter rh type c; PDBTitle: crystal structure of the human rhesus glycoprotein rhcg 12 c1odrA_ 7.7 22 PDB header:lipid transport Chain: A: PDB Molecule:apoa-i peptide; PDBTitle: peptide of human apoa-i residues 166-185. nmr, 5 structures2 at ph 6.0, 37 degrees celsius and peptide:dpc mole ratio3 of 1:40 13 c1odqA_ 7.7 22 PDB header:lipid transport Chain: A: PDB Molecule:apoa-i peptide; PDBTitle: peptide of human apoa-i residues 166-185. nmr, 5 structures2 at ph 3.7, 37 degrees celsius and peptide:sds mole ratio3 of 1:40 14 c1odpA_ 7.7 22 PDB header:lipid transport Chain: A: PDB Molecule:apoa-i peptide; PDBTitle: peptide of human apoa-i residues 166-185. nmr, 5 structures2 at ph 6.6, 37 degrees celsius and peptide:sds mole ratio3 of 1:40 15 d1vkza1 6.0 13 Fold:Barrel-sandwich hybrid Superfamily:Rudiment single hybrid motif Family:BC C-terminal domain-like 16 d1rh1a2 5.5 13 Fold:Toxins' membrane translocation domains Superfamily:Colicin Family:Colicin 17 c2kncB_ 5.3 13 PDB header:cell adhesion Chain: B: PDB Molecule:integrin beta-3; PDBTitle: platelet integrin alfaiib-beta3 transmembrane-cytoplasmic2 heterocomplex

Binding site prediction

Due to computational demand, binding site predictions are not run for batch jobs

If you want to predict binding sites, please manually submit your model of choice to 3DLigandSite

Transmembrane helix prediction

Transmembrane helices have been predicted in your sequence to adopt the topology shown below

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Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
If you use the binding site predictions from 3DLigandSite, please also cite:
3DLigandSite: predicting ligand-binding sites using similar structures.
Wass MN, Kelley LA and Sternberg MJ Nucleic Acids Research 38, W469-73 (2010) [PubMed]
© Structural Bioinformatics Group Imperial College London Lawrence Kelley, Benjamin Jefferys Disclaimer Terms and Conditions	Component software Template detection: HHpred 1.51 Secondary structure prediction: Psi-pred 2.5 Disorder prediction: Disopred 2.4 Transmembrane prediction: Memsat_SVM Multi-template modelling and ab initio: Poing 1.0