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Job DescriptionP31447
Confidence100.00%DateThu Jan 5 11:47:37 GMT 2012
Rank13Aligned Residues313
% Identity18%Templatec3q3qA_
PDB info PDB header:hydrolaseChain: A: PDB Molecule:alkaline phosphatase; PDBTitle: crystal structure of spap: an novel alkaline phosphatase from2 bacterium sphingomonas sp. strain bsar-1
Resolution1.95 Å

  Insertion relative to template
  Deletion relative to template
  Catalytic residue from the CSA
 
Detailed help on interpreting your alignment


   2... ....10.........20.........30.........40.........50 .........60.........70....
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Query Sequence  KRPN. FLFVMTDTQATNMVGCYSGKPLNTQNIDSLAAEGIRFNSAYTCSP. . VCTPARAGLFTGIYANQSGPWTNN. . . .
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Template Sequence  TPPKLIVAISVDQFSADLFSEYRQYY. . TGGLKRLTSEGAVFPRGYQSHAATETCPGHSTILTGSRPSRTGIIANNWFDL
Template Known Secondary structure  S

STT

GGG
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SS

SS

STTS




SS
T
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   38.40.........50.........60... ......70.........80.........90.........100.........110.....
 
   75....80.........90.........100.........110.......
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Query Sequence  . . . . . . . . . . . . . . . . . . . . . . . . . . . VAPGKNISTMGRYFKDAGYHTCYIGKWHLDGHDYFGTGECPPE. . . . . . . . . .
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Template Sequence  DAKREDKNLYCAEDESQPGSSSDKYEASPLHLKVPTLGGRMKAANPATRVVSVAGKDRAAIMMGGATADQVWWLGGPQGY
Template Known Secondary structure  T
SSS

TTB
TTSTT
BTTB

B
STT

S


TT

SS
TT
S
STT
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   116...120.........130.........140.........150.........160.........170.........180.........190.....
 
   118.120.........130.........140.........150.........160.........170.
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Query Sequence  . . . . . . . . . . . . . . . . . . . . . . . . . . WDADYWFDGANYLSELTEKEISLWRNGLNSVEDLQANHIDETFTWAHRISNRAV
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Template Sequence  VSYKGVAPTPLVTQVNQAFAQRLAQPNPGFELPAQCVSKDFPVQAGNRTVGTGRFARDAGDYKGFRISPEQDAMTLAFAA
Template Known Secondary structure 
STT



TS
B





GGGGGG


TTS




B
TT
SS
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   196...200.........210.........220.........230.........240.........250.........260.........270.....
 
   172.... ...180.........190.........200.........210.........220.........230.........240.........
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Query Sequence  DFLQQ. . PARADEPFLMVVSYDEPHHPFTCPVEYLEKYADFYYELGEKAQDDLANKPEHHRLWAQAMPSPVGDDGLYHHP
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Template Conservation               
          
   
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Template Sequence  AAIENMQLGKQAQTDIISIGLSATDYVGH. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . TFGTEGT
Template Known Secondary structure  TTTTSSSS

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SSS
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   276...280.........290.........300.... .....310.
 
   250.........260.........270.........280......
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Query Sequence  LYFACNDFVDDQIGRVINALTPEQRENTWVIYTSDHG. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
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Template Sequence  ESCIQVDRLDTELGAFFDKLDKDG. IDYVVVLTADHGGHDLPERHRMNAMPMEQRVDMALTPKALNATIAEKAGLPGKKV
Template Known Secondary structure  TT.


S





TTTT
TT


GGGST
TTS

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   312.......320.........330..... ....340.........350.........360.........370.........380.........390
 
  
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Query Sequence  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Query Conservation  ................................................................................
Template Conservation           
                                                                 


  
Template Sequence  IWSDGPSGDIYYDKGLTAAQRARVETEALKYLRAHPQVQTVFTKAEIAATPSPSGPPESWSLIQEARASFYPSRSGDLLL
Template Known Secondary structure  SSSS
TT

STTTS



S
GGG

T

TTTS
S
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   391........400.........410.........420.........430.........440.........450.........460.........470
 
   287..290.........300.........310.........320.........330.........340...... ...350......
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Query Sequence  . . . . . . . . . EMMGAHKLISKGAAMYDDITRIPLIIRSPQGERRQVDTPVSHIDLLPTMMALADIEKPEI. LPGENILAVK
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Template Sequence  LLKPRVMSIPEQAVMGSVATHGSPWDTDRRVPILFWRKGMQHFEQPLGVETVDILPSLAALIKLPVPKDQIDGRCLDLVA
Template Known Secondary structure 
TTSS


SS
S






SSTT




S
GGGTT



GGG
S





S
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   471........480.........490.........500.........510.........520.........530.........540.........550
 
   357..360.
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Query Sequence  EPRGV
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Template Sequence  GKDDS
Template Known Secondary structure  SS


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   551....
 
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Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
 
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Lawrence Kelley, Benjamin Jefferys 
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