PHYRE Protein Fold Recognition Server

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Protein Homology/analogY Recognition Engine V 2.2

Fold library entry viewer: c5axwA_

Fold library id	PDB Header	Molecule	Title
c5axwA_	PDB header: hydrolase/rna/dna	Chain: A: PDB Molecule: crispr-associated endonuclease cas9;	PDBTitle: crystal structure of staphylococcus aureus cas9 in complex with sgrna2 and target dna (ttgggt pam)

Links to external resources
Added to library: Sat Sep 5 08:28:47 2015

1 . . . . . . . . 10 . . . . . . . . . 20 . . . . . . . . . 30 . . . . . . . . . 40 . . . . . . . . . 50 . . . . . . . . . 60 . . . . . . . . . 70

Sequence R N Y I L G L D I G I T S V G Y G I I D Y E T R D V I D A G V R L F K E A N V E N N E G R R S K R G A R R L K R R R R H R I Q R V K K L L F

Predicted secondary structure

SS confidence

Known secondary structure (DSSP)
S S T T T

. . . . . . . . . 80 . . . . . . . . . 90 . . . . . . . . . 100 . . . . . . . . . 110 . . . . . . . . . 120 . . . . . . . . . 130 . . . . . . . . . 140

Sequence D Y N L L T D H S E L S G I N P Y E A R V K G L S Q K L S E E E F S A A L L H L A K R R G V H N V N E V E E D T G N E L S T K E Q I S R N S

Predicted secondary structure

SS confidence

Known secondary structure (DSSP) T T S
S S
S S
T T T S
B S S G G G
S

. . . . . . . . . 150 . . . . . . . . . 160 . . . . . . . . . 170 . . . . . . . . . 180 . . . . . . . . . 190 . . . . . . . . . 200 . . . . . . . . . 210

Sequence K A L E E K Y V A E L Q L E R L K K D G E V R G S I N R F K T S D Y V K E A K Q L L K V Q K A Y H Q L D Q S F I D T Y I D L L E T R R T Y Y

Predicted secondary structure

SS confidence

Known secondary structure (DSSP) T T S S S
S
S S S
B T T T S
T T

. . . . . . . . . 220 . . . . . . . . . 230 . . . . . . . . . 240 . . . . . . . . . 250 . . . . . . . . . 260 . . . . . . . . . 270 . . . . . . . . . 280

Sequence E G P G E G S P F G W K D I K E W Y E M L M G H C T Y F P E E L R S V K Y A Y N A D L Y N A L N D L N N L V I T R D E N E K L E Y Y E K F Q

Predicted secondary structure

SS confidence

Known secondary structure (DSSP) T
S
S S
T T
S S S
B
S S
T T S B
B T T
T
S S S
S S

. . . . . . . . . 290 . . . . . . . . . 300 . . . . . . . . . 310 . . . . . . . . . 320 . . . . . . . . . 330 . . . . . . . . . 340 . . . . . . . . . 350

Sequence I I E N V F K Q K K K P T L K Q I A K E I L V N E E D I K G Y R V T S T G K P E F T N L K V Y H D I K D I T A R K E I I E N A E L L D Q I A

Predicted secondary structure

SS confidence

Known secondary structure (DSSP) T T T T
S S
T S
G G G
B
T T S
B
T

. . . . . . . . . 360 . . . . . . . . . 370 . . . . . . . . . 380 . . . . . . . . . 390 . . . . . . . . . 400 . . . . . . . . . 410 . . . . . . . . . 420

Sequence K I L T I Y Q S S E D I Q E E L T N L N S E L T Q E E I E Q I S N L K G Y T G T H N L S L K A I N L I L D E L W H T N D N Q I A I F N R L K

Predicted secondary structure

SS confidence

Known secondary structure (DSSP)
S T T
S
T T
S B
S S
T S
T T

. . . . . . . . . 430 . . . . . . . . . 440 . . . . . . . . . 450 . . . . . . . . . 460 . . . . . . . . . 470 . . . . . . . . . 480 . . . . . . . . . 490

Sequence L V P K K V D L S Q Q K E I P T T L V D D F I L S P V V K R S F I Q S I K V I N A I I K K Y G L P N D I I I E L A R E K N S K D A Q K M I N

Predicted secondary structure

SS confidence

Known secondary structure (DSSP)
S S
S S
S G G G G G G
S
S
S S

. . . . . . . . . 500 . . . . . . . . . 510 . . . . . . . . . 520 . . . . . . . . . 530 . . . . . . . . . 540 . . . . . . . . . 550 . . . . . . . . . 560

Sequence E M Q K R N R Q T N E R I E E I I R T T G K E N A K Y L I E K I K L H D M Q E G K C L Y S L E A I P L E D L L N N P F N Y E V D H I I P R S

Predicted secondary structure

SS confidence

Known secondary structure (DSSP) T
G G G G G
T T T B
T T T
B
G G G S S

. . . . . . . . . 570 . . . . . . . . . 580 . . . . . . . . . 590 . . . . . . . . . 600 . . . . . . . . . 610 . . . . . . . . . 620 . . . . . . . . . 630

Sequence V S F D N S F N N K V L V K Q E E A S K K G N R T P F Q Y L S S S D S K I S Y E T F K K H I L N L A K G K G R I S K T K K E Y L L E E R D I

Predicted secondary structure

SS confidence

Known secondary structure (DSSP)
S G G G
S S
S T T
S S
T S S T T
T
S
T

. . . . . . . . . 640 . . . . . . . . . 650 . . . . . . . . . 660 . . . . . . . . . 670 . . . . . . . . . 680 . . . . . . . . . 690 . . . . . . . . . 700

Sequence N R F S V Q K D F I N R N L V D T R Y A T R G L M N L L R S Y F R V N N L D V K V K S I N G G F T S F L R R K W K F K K E R N K G Y K H H A

Predicted secondary structure

SS confidence

Known secondary structure (DSSP) T
T S
T T
T T
S S
S
T

. . . . . . . . . 710 . . . . . . . . . 720 . . . . . . . . . 730 . . . . . . . . . 740 . . . . . . . . . 750 . . . . . . . . . 760 . . . . . . . . . 770

Sequence E D A L I I A N A D F I F K E W K K L D K A K K V M E N Q M F M P E I E T E Q E Y K E I F I T P H Q I K H I K D F K D Y K Y S H R V D K K P

Predicted secondary structure

SS confidence

Known secondary structure (DSSP)
S T T T T
S
G G G
B
S

. . . . . . . . . 780 . . . . . . . . . 790 . . . . . . . . . 800 . . . . . . . . . 810 . . . . . . . . . 820 . . . . . . . . . 830 . . . . . . . . . 840

Sequence N R E L I N D T L Y S T R K D D K G N T L I V N N L N G L Y D K D N D K L K K L I N K S P E K L L M Y H H D P Q T Y Q K L K L I M E Q Y G D

Predicted secondary structure

SS confidence

Known secondary structure (DSSP) S S
B
T T S
T T
T T
G G G B G G G T S
T T T

. . . . . . . . . 850 . . . . . . . . . 860 . . . . . . . . . 870 . . . . . . . . . 880 . . . . . . . . . 890 . . . . . . . . . 900 . . . . . . . . . 910

Sequence E K N P L Y K Y Y E E T G N Y L T K Y S K K D N G P V I K K I K Y Y G N K L N A H L D I T D D Y P N S R N K V V K L S L K P Y R F D V Y L D

Predicted secondary structure

SS confidence

Known secondary structure (DSSP) S S
S
B
T T S
B
S
G G G S T T
S S
B

. . . . . . . . . 920 . . . . . . . . . 930 . . . . . . . . . 940 . . . . . . . . . 950 . . . . . . . . . 960 . . . . . . . . . 970 . . . . . . . . . 980

Sequence N G V Y K F V T V K N L D V I K K E N Y Y E V N S K A Y E E A K K L K K I S N Q A E F I A S F Y N N D L I K I N G E L Y R V I G V N N D L L

Predicted secondary structure

SS confidence

Known secondary structure (DSSP) T T G G G
S S
T T
S S
B T
T T T T T

. . . . . . . . . 990 . . . . . . . . . 1000 . . . . . . . . . 1010 . . . . . . . . . 1020 . . . . . . . . . 1030 . . . . . . . . . 1040 . . .

Sequence N R I E V N M I D I T Y R E Y L E N M N D K R P P R I I K T I A S K T Q S I K K Y S T D I L G N L Y E V K S K K H P Q I I K K

Predicted secondary structure

SS confidence

Known secondary structure (DSSP) T
S S S
T T
S
S
S T T
T T
S S

Download: PDB structure FASTA sequence
Hidden Markov model

Image coloured by rainbow N → C terminus

Interactive 3D view in JSmol

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All images and data generated by Phyre2 are free to use in any publication with acknowledgement
Accessibility Statement

Please cite: Phyre2.2: A Community Resource for Template-based Protein Structure Prediction

Powell HR et al. Journal of Molecular Biology (2025) in press DOI: https://doi.org/10.1016/j.jmb.2025.168960

© Structural Bioinformatics Group, Imperial College, London

Michael Sternberg

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Phyre2 is part of Genome3D

	1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60	.	.	.	.	.	.	.	.	.	70
Sequence	R	N	Y	I	L	G	L	D	I	G	I	T	S	V	G	Y	G	I	I	D	Y	E	T	R	D	V	I	D	A	G	V	R	L	F	K	E	A	N	V	E	N	N	E	G	R	R	S	K	R	G	A	R	R	L	K	R	R	R	R	H	R	I	Q	R	V	K	K	L	L	F
Predicted secondary structure
SS confidence
Known secondary structure (DSSP)											S	S									T	T	T
	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120	.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140
Sequence	D	Y	N	L	L	T	D	H	S	E	L	S	G	I	N	P	Y	E	A	R	V	K	G	L	S	Q	K	L	S	E	E	E	F	S	A	A	L	L	H	L	A	K	R	R	G	V	H	N	V	N	E	V	E	E	D	T	G	N	E	L	S	T	K	E	Q	I	S	R	N	S
Predicted secondary structure
SS confidence
Known secondary structure (DSSP)		T	T	S			S	S				S	S										T	T	T	S																				B	S	S	G	G	G							S
	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180	.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210
Sequence	K	A	L	E	E	K	Y	V	A	E	L	Q	L	E	R	L	K	K	D	G	E	V	R	G	S	I	N	R	F	K	T	S	D	Y	V	K	E	A	K	Q	L	L	K	V	Q	K	A	Y	H	Q	L	D	Q	S	F	I	D	T	Y	I	D	L	L	E	T	R	R	T	Y	Y
Predicted secondary structure
SS confidence
Known secondary structure (DSSP)		T	T	S	S	S														S				S	S	S				B																T	T	T	S																				T	T
	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240	.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280
Sequence	E	G	P	G	E	G	S	P	F	G	W	K	D	I	K	E	W	Y	E	M	L	M	G	H	C	T	Y	F	P	E	E	L	R	S	V	K	Y	A	Y	N	A	D	L	Y	N	A	L	N	D	L	N	N	L	V	I	T	R	D	E	N	E	K	L	E	Y	Y	E	K	F	Q
Predicted secondary structure
SS confidence
Known secondary structure (DSSP)	T		S		S	S		T	T			S	S									S		B		S	S		T	T	S	B			B	T	T															T					S	S	S		S	S
	.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300	.	.	.	.	.	.	.	.	.	310	.	.	.	.	.	.	.	.	.	320	.	.	.	.	.	.	.	.	.	330	.	.	.	.	.	.	.	.	.	340	.	.	.	.	.	.	.	.	.	350
Sequence	I	I	E	N	V	F	K	Q	K	K	K	P	T	L	K	Q	I	A	K	E	I	L	V	N	E	E	D	I	K	G	Y	R	V	T	S	T	G	K	P	E	F	T	N	L	K	V	Y	H	D	I	K	D	I	T	A	R	K	E	I	I	E	N	A	E	L	L	D	Q	I	A
Predicted secondary structure
SS confidence
Known secondary structure (DSSP)					T	T	T	T		S	S											T	S		G	G	G						B		T	T	S		B																						T
	.	.	.	.	.	.	.	.	.	360	.	.	.	.	.	.	.	.	.	370	.	.	.	.	.	.	.	.	.	380	.	.	.	.	.	.	.	.	.	390	.	.	.	.	.	.	.	.	.	400	.	.	.	.	.	.	.	.	.	410	.	.	.	.	.	.	.	.	.	420
Sequence	K	I	L	T	I	Y	Q	S	S	E	D	I	Q	E	E	L	T	N	L	N	S	E	L	T	Q	E	E	I	E	Q	I	S	N	L	K	G	Y	T	G	T	H	N	L	S	L	K	A	I	N	L	I	L	D	E	L	W	H	T	N	D	N	Q	I	A	I	F	N	R	L	K
Predicted secondary structure
SS confidence
Known secondary structure (DSSP)								S										T	T			S										T	T						S	B		S	S								T							S											T	T
	.	.	.	.	.	.	.	.	.	430	.	.	.	.	.	.	.	.	.	440	.	.	.	.	.	.	.	.	.	450	.	.	.	.	.	.	.	.	.	460	.	.	.	.	.	.	.	.	.	470	.	.	.	.	.	.	.	.	.	480	.	.	.	.	.	.	.	.	.	490
Sequence	L	V	P	K	K	V	D	L	S	Q	Q	K	E	I	P	T	T	L	V	D	D	F	I	L	S	P	V	V	K	R	S	F	I	Q	S	I	K	V	I	N	A	I	I	K	K	Y	G	L	P	N	D	I	I	I	E	L	A	R	E	K	N	S	K	D	A	Q	K	M	I	N
Predicted secondary structure
SS confidence
Known secondary structure (DSSP)									S	S		S	S			S	G	G	G	G	G	G																												S								S		S	S
	.	.	.	.	.	.	.	.	.	500	.	.	.	.	.	.	.	.	.	510	.	.	.	.	.	.	.	.	.	520	.	.	.	.	.	.	.	.	.	530	.	.	.	.	.	.	.	.	.	540	.	.	.	.	.	.	.	.	.	550	.	.	.	.	.	.	.	.	.	560
Sequence	E	M	Q	K	R	N	R	Q	T	N	E	R	I	E	E	I	I	R	T	T	G	K	E	N	A	K	Y	L	I	E	K	I	K	L	H	D	M	Q	E	G	K	C	L	Y	S	L	E	A	I	P	L	E	D	L	L	N	N	P	F	N	Y	E	V	D	H	I	I	P	R	S
Predicted secondary structure
SS confidence
Known secondary structure (DSSP)																				T			G	G	G	G	G											T	T	T	B		T	T	T			B										G	G	G						S	S
	.	.	.	.	.	.	.	.	.	570	.	.	.	.	.	.	.	.	.	580	.	.	.	.	.	.	.	.	.	590	.	.	.	.	.	.	.	.	.	600	.	.	.	.	.	.	.	.	.	610	.	.	.	.	.	.	.	.	.	620	.	.	.	.	.	.	.	.	.	630
Sequence	V	S	F	D	N	S	F	N	N	K	V	L	V	K	Q	E	E	A	S	K	K	G	N	R	T	P	F	Q	Y	L	S	S	S	D	S	K	I	S	Y	E	T	F	K	K	H	I	L	N	L	A	K	G	K	G	R	I	S	K	T	K	K	E	Y	L	L	E	E	R	D	I
Predicted secondary structure
SS confidence
Known secondary structure (DSSP)						S	G	G	G														S	S								S	T	T		S	S													T	S	S	T	T											T			S		T
	.	.	.	.	.	.	.	.	.	640	.	.	.	.	.	.	.	.	.	650	.	.	.	.	.	.	.	.	.	660	.	.	.	.	.	.	.	.	.	670	.	.	.	.	.	.	.	.	.	680	.	.	.	.	.	.	.	.	.	690	.	.	.	.	.	.	.	.	.	700
Sequence	N	R	F	S	V	Q	K	D	F	I	N	R	N	L	V	D	T	R	Y	A	T	R	G	L	M	N	L	L	R	S	Y	F	R	V	N	N	L	D	V	K	V	K	S	I	N	G	G	F	T	S	F	L	R	R	K	W	K	F	K	K	E	R	N	K	G	Y	K	H	H	A
Predicted secondary structure
SS confidence
Known secondary structure (DSSP)	T													T	S																				T	T																				T	T			S	S			S		T
	.	.	.	.	.	.	.	.	.	710	.	.	.	.	.	.	.	.	.	720	.	.	.	.	.	.	.	.	.	730	.	.	.	.	.	.	.	.	.	740	.	.	.	.	.	.	.	.	.	750	.	.	.	.	.	.	.	.	.	760	.	.	.	.	.	.	.	.	.	770
Sequence	E	D	A	L	I	I	A	N	A	D	F	I	F	K	E	W	K	K	L	D	K	A	K	K	V	M	E	N	Q	M	F	M	P	E	I	E	T	E	Q	E	Y	K	E	I	F	I	T	P	H	Q	I	K	H	I	K	D	F	K	D	Y	K	Y	S	H	R	V	D	K	K	P
Predicted secondary structure
SS confidence
Known secondary structure (DSSP)																	S	T	T								T	T									S											G	G	G																	B			S
	.	.	.	.	.	.	.	.	.	780	.	.	.	.	.	.	.	.	.	790	.	.	.	.	.	.	.	.	.	800	.	.	.	.	.	.	.	.	.	810	.	.	.	.	.	.	.	.	.	820	.	.	.	.	.	.	.	.	.	830	.	.	.	.	.	.	.	.	.	840
Sequence	N	R	E	L	I	N	D	T	L	Y	S	T	R	K	D	D	K	G	N	T	L	I	V	N	N	L	N	G	L	Y	D	K	D	N	D	K	L	K	K	L	I	N	K	S	P	E	K	L	L	M	Y	H	H	D	P	Q	T	Y	Q	K	L	K	L	I	M	E	Q	Y	G	D
Predicted secondary structure
SS confidence
Known secondary structure (DSSP)	S	S			B											T	T	S											T	T		T	T												G	G	G	B	G	G	G	T	S															T	T	T
	.	.	.	.	.	.	.	.	.	850	.	.	.	.	.	.	.	.	.	860	.	.	.	.	.	.	.	.	.	870	.	.	.	.	.	.	.	.	.	880	.	.	.	.	.	.	.	.	.	890	.	.	.	.	.	.	.	.	.	900	.	.	.	.	.	.	.	.	.	910
Sequence	E	K	N	P	L	Y	K	Y	Y	E	E	T	G	N	Y	L	T	K	Y	S	K	K	D	N	G	P	V	I	K	K	I	K	Y	Y	G	N	K	L	N	A	H	L	D	I	T	D	D	Y	P	N	S	R	N	K	V	V	K	L	S	L	K	P	Y	R	F	D	V	Y	L	D
Predicted secondary structure
SS confidence
Known secondary structure (DSSP)	S	S											S				B				T	T	S				B													S					G	G	G	S	T	T		S	S							B
	.	.	.	.	.	.	.	.	.	920	.	.	.	.	.	.	.	.	.	930	.	.	.	.	.	.	.	.	.	940	.	.	.	.	.	.	.	.	.	950	.	.	.	.	.	.	.	.	.	960	.	.	.	.	.	.	.	.	.	970	.	.	.	.	.	.	.	.	.	980
Sequence	N	G	V	Y	K	F	V	T	V	K	N	L	D	V	I	K	K	E	N	Y	Y	E	V	N	S	K	A	Y	E	E	A	K	K	L	K	K	I	S	N	Q	A	E	F	I	A	S	F	Y	N	N	D	L	I	K	I	N	G	E	L	Y	R	V	I	G	V	N	N	D	L	L
Predicted secondary structure
SS confidence
Known secondary structure (DSSP)	T	T									G	G	G					S	S																T	T			S	S									B	T						T	T											T	T	T
	.	.	.	.	.	.	.	.	.	990	.	.	.	.	.	.	.	.	.	1000	.	.	.	.	.	.	.	.	.	1010	.	.	.	.	.	.	.	.	.	1020	.	.	.	.	.	.	.	.	.	1030	.	.	.	.	.	.	.	.	.	1040	.	.	.
Sequence	N	R	I	E	V	N	M	I	D	I	T	Y	R	E	Y	L	E	N	M	N	D	K	R	P	P	R	I	I	K	T	I	A	S	K	T	Q	S	I	K	K	Y	S	T	D	I	L	G	N	L	Y	E	V	K	S	K	K	H	P	Q	I	I	K	K
Predicted secondary structure
SS confidence
Known secondary structure (DSSP)	T							S	S	S									T	T			S		S							S	T	T											T	T									S	S