Email l.a.kelley@imperial.ac.uk Description C6Y4A9 Date Sun Jul 8 11:45:06 BST 2012 Unique Job ID cb512630c2bffacc

Summary

Top model

Image coloured by rainbow N → C terminus Close Jmol

Model (left) based on template c1tinA_ Top template information PDB header:serine protease inhibitor Chain: A: PDB Molecule:trypsin inhibitor v; PDBTitle: three-dimensional structure in solution of cucurbita maxima2 trypsin inhibitor-v determined by nmr spectroscopy Confidence and coverage Confidence: 97.8% Coverage: 68% 44 residues ( 68% of your sequence) have been modelled with 97.8% confidence by the single highest scoring template. You may wish to submit your sequence to Phyrealarm. This will automatically scan your sequence every week for new potential templates as they appear in the Phyre2 library. 3D viewing Interactive 3D view in Jmol

Sequence analysis

Secondary structure and disorder prediction

Confidence Key
High(9)											Low (0)

?	Disordered
	Alpha helix
	Beta strand

Domain analysis

Hover over an aligned region to see model and summary info

Please note, only up to the top 20 hits are modelled to reduce computer load

Rank Aligned region

PDB 1tin chain A Region: 19 - 62 Aligned: 44 Modelled: 44 Confidence: 97.8% Identity: 18% PDB header:serine protease inhibitor Chain: A: PDB Molecule:trypsin inhibitor v; PDBTitle: three-dimensional structure in solution of cucurbita maxima2 trypsin inhibitor-v determined by nmr spectroscopy Phyre2 PDB 1dwm chain A Region: 19 - 62 Aligned: 44 Modelled: 44 Confidence: 97.8% Identity: 34% Fold: CI-2 family of serine protease inhibitors Superfamily: CI-2 family of serine protease inhibitors Family: CI-2 family of serine protease inhibitors Phyre2 PDB 1vbw chain A Region: 19 - 62 Aligned: 44 Modelled: 44 Confidence: 97.8% Identity: 20% PDB header:protein binding Chain: A: PDB Molecule:trypsin inhibitor bgit; PDBTitle: crystal structure of bitter gourd trypsin inhibitor Phyre2 PDB 2ci2 chain I Region: 19 - 62 Aligned: 44 Modelled: 44 Confidence: 97.7% Identity: 20% PDB header:proteinase inhibitor (chymotrypsin) Chain: I: PDB Molecule:chymotrypsin inhibitor 2; PDBTitle: crystal and molecular structure of the serine proteinase2 inhibitor ci-2 from barley seeds Phyre2 PDB 3rdy chain A Region: 19 - 62 Aligned: 44 Modelled: 44 Confidence: 97.7% Identity: 25% PDB header:hydrolase inhibitor Chain: A: PDB Molecule:bwi-1=protease inhibitor/trypsin inhibitor; PDBTitle: crystal structure of buckwheat trypsin inhibitor rbti at 1.84 angstrom2 resolution Phyre2 PDB 1ypc chain I Region: 19 - 62 Aligned: 44 Modelled: 44 Confidence: 97.7% Identity: 20% Fold: CI-2 family of serine protease inhibitors Superfamily: CI-2 family of serine protease inhibitors Family: CI-2 family of serine protease inhibitors Phyre2 PDB 1to2 chain I Region: 19 - 62 Aligned: 44 Modelled: 44 Confidence: 97.7% Identity: 20% Fold: CI-2 family of serine protease inhibitors Superfamily: CI-2 family of serine protease inhibitors Family: CI-2 family of serine protease inhibitors Phyre2 PDB 2sni chain I Region: 19 - 62 Aligned: 44 Modelled: 44 Confidence: 97.7% Identity: 20% Fold: CI-2 family of serine protease inhibitors Superfamily: CI-2 family of serine protease inhibitors Family: CI-2 family of serine protease inhibitors Phyre2 PDB 1cse chain I Region: 19 - 62 Aligned: 44 Modelled: 44 Confidence: 97.6% Identity: 20% Fold: CI-2 family of serine protease inhibitors Superfamily: CI-2 family of serine protease inhibitors Family: CI-2 family of serine protease inhibitors Phyre2 PDB 1egl chain A Region: 19 - 62 Aligned: 44 Modelled: 44 Confidence: 97.6% Identity: 20% Fold: CI-2 family of serine protease inhibitors Superfamily: CI-2 family of serine protease inhibitors Family: CI-2 family of serine protease inhibitors Phyre2 PDB 1ciq chain B Region: 43 - 62 Aligned: 20 Modelled: 20 Confidence: 32.9% Identity: 30% PDB header:serine protease inhibitor Chain: B: PDB Molecule:chymotrypsin inhibitor 2; PDBTitle: complex of two fragments of ci2, residues 1-40 and 41-64 Phyre2 PDB 1cq4 chain B Region: 44 - 62 Aligned: 19 Modelled: 19 Confidence: 19.5% Identity: 32% PDB header:hydrolase inhibitor Chain: B: PDB Molecule:protein (serine proteinase inhibitor 2); PDBTitle: ci2 mutant with tetraglutamine (mgqqqqgm) replacing met59 Phyre2 PDB 3h3h chain A Region: 51 - 64 Aligned: 14 Modelled: 14 Confidence: 14.2% Identity: 14% PDB header:unknown function Chain: A: PDB Molecule:uncharacterized snoal-like protein; PDBTitle: crystal structure of a snoal-like protein of unknown function2 (bth_ii0226) from burkholderia thailandensis e264 at 1.60 a3 resolution Phyre2 PDB 2l55 chain A Region: 39 - 63 Aligned: 25 Modelled: 25 Confidence: 12.5% Identity: 28% PDB header:metal binding protein Chain: A: PDB Molecule:silb,silver efflux protein, mfp component of the three PDBTitle: solution structure of the c-terminal domain of silb from cupriavidus2 metallidurans Phyre2 PDB 1fxz chain A domain 2 Region: 10 - 65 Aligned: 56 Modelled: 56 Confidence: 11.6% Identity: 14% Fold: Double-stranded beta-helix Superfamily: RmlC-like cupins Family: Germin/Seed storage 7S protein Phyre2 PDB 3dxo chain A domain 1 Region: 51 - 63 Aligned: 13 Modelled: 13 Confidence: 10.6% Identity: 23% Fold: Cystatin-like Superfamily: NTF2-like Family: Atu0744-like Phyre2 PDB 1hym chain B Region: 43 - 61 Aligned: 19 Modelled: 19 Confidence: 9.3% Identity: 26% PDB header:hydrolase (serine proteinase) Chain: B: PDB Molecule:hydrolyzed cucurbita maxima trypsin inhibitor v; PDBTitle: hydrolyzed trypsin inhibitor (cmti-v, minimized average nmr2 structure) Phyre2 PDB 3lti chain A Region: 13 - 48 Aligned: 36 Modelled: 36 Confidence: 8.0% Identity: 31% PDB header:transferase Chain: A: PDB Molecule:dna-directed rna polymerase subunit beta; PDBTitle: crystal structure of the escherichia coli rna polymerase beta subunit2 beta2-betai4 domains Phyre2 PDB 1qcs chain A domain 2 Region: 33 - 53 Aligned: 21 Modelled: 21 Confidence: 7.2% Identity: 5% Fold: Cdc48 domain 2-like Superfamily: Cdc48 domain 2-like Family: Cdc48 domain 2-like Phyre2 PDB 2hx6 chain A Region: 43 - 51 Aligned: 9 Modelled: 9 Confidence: 6.6% Identity: 22% PDB header:hydrolase Chain: A: PDB Molecule:ribonuclease; PDBTitle: solution structure analysis of the phage t42 endoribonuclease regb Phyre2 1 c1tinA_ 2 d1dwma_ 3 c1vbwA_ 4 c2ci2I_ 5 c3rdyA_ 6 d1ypci_ 7 d1to2i_ 8 d2snii_ 9 d1csei_ 10 d1egla_ 11 c1ciqB_ 12 c1cq4B_ 13 c3h3hA_ 14 c2l55A_ 15 d1fxza2 16 d3dxoa1 17 c1hymB_ 18 c3ltiA_ 19 d1qcsa2 20 c2hx6A_ 21 22 23 24 25 26 27 28 29 30 31 32

Binding site prediction

Due to computational demand, binding site predictions are not run for batch jobs

If you want to predict binding sites, please manually submit your model of choice to 3DLigandSite

Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
If you use the binding site predictions from 3DLigandSite, please also cite:
3DLigandSite: predicting ligand-binding sites using similar structures.
Wass MN, Kelley LA and Sternberg MJ Nucleic Acids Research 38, W469-73 (2010) [PubMed]
© Structural Bioinformatics Group Imperial College London Lawrence Kelley, Benjamin Jefferys Disclaimer Terms and Conditions	Component software Template detection: HHpred 1.51 Secondary structure prediction: Psi-pred 2.5 Disorder prediction: Disopred 2.4 Transmembrane prediction: Memsat_SVM Multi-template modelling and ab initio: Poing 1.0