Email l.a.kelley@imperial.ac.uk Description C6Y4A3 Date Sun Jul 8 11:45:04 BST 2012 Unique Job ID 6ce861c8a4fcff67

Summary

Top model

Image coloured by rainbow N → C terminus Close Jmol

Model (left) based on template d1ej5a_ Top template information Fold:Wiscott-Aldrich syndrome protein, WASP, C-terminal domain Superfamily:Wiscott-Aldrich syndrome protein, WASP, C-terminal domain Family:Wiscott-Aldrich syndrome protein, WASP, C-terminal domain Confidence and coverage Confidence: 28.1% Coverage: 24% 16 residues ( 24% of your sequence) have been modelled with 28.1% confidence by the single highest scoring template. You may wish to submit your sequence to Phyrealarm. This will automatically scan your sequence every week for new potential templates as they appear in the Phyre2 library. Please note: You must be registered and logged in to use Phyrealarm. 3D viewing Interactive 3D view in Jmol

Sequence analysis

Secondary structure and disorder prediction

Confidence Key
High(9)											Low (0)

?	Disordered
	Alpha helix
	Beta strand

Domain analysis

Hover over an aligned region to see model and summary info

Please note, only up to the top 20 hits are modelled to reduce computer load

Rank Aligned region

PDB 1ej5 chain A Region: 52 - 67 Aligned: 16 Modelled: 16 Confidence: 28.1% Identity: 25% Fold: Wiscott-Aldrich syndrome protein, WASP, C-terminal domain Superfamily: Wiscott-Aldrich syndrome protein, WASP, C-terminal domain Family: Wiscott-Aldrich syndrome protein, WASP, C-terminal domain Phyre2 PDB 2p22 chain C Region: 51 - 66 Aligned: 16 Modelled: 16 Confidence: 20.9% Identity: 19% PDB header:transport protein Chain: C: PDB Molecule:protein srn2; PDBTitle: structure of the yeast escrt-i heterotetramer core Phyre2 PDB 2k42 chain A Region: 52 - 67 Aligned: 16 Modelled: 16 Confidence: 16.5% Identity: 25% PDB header:signaling protein Chain: A: PDB Molecule:wiskott-aldrich syndrome protein; PDBTitle: solution structure of the gtpase binding domain of wasp in2 complex with espfu, an ehec effector Phyre2 PDB 1gvd chain A Region: 53 - 67 Aligned: 15 Modelled: 15 Confidence: 15.0% Identity: 27% Fold: DNA/RNA-binding 3-helical bundle Superfamily: Homeodomain-like Family: Myb/SANT domain Phyre2 PDB 1guu chain A Region: 53 - 67 Aligned: 15 Modelled: 15 Confidence: 14.0% Identity: 33% Fold: DNA/RNA-binding 3-helical bundle Superfamily: Homeodomain-like Family: Myb/SANT domain Phyre2 PDB 1guu chain A Region: 53 - 67 Aligned: 15 Modelled: 15 Confidence: 14.0% Identity: 33% PDB header:transcription Chain: A: PDB Molecule:myb proto-oncogene protein; PDBTitle: crystal structure of c-myb r1 Phyre2 PDB 1ea0 chain A domain 1 Region: 52 - 67 Aligned: 16 Modelled: 16 Confidence: 13.3% Identity: 31% Fold: Single-stranded right-handed beta-helix Superfamily: Alpha subunit of glutamate synthase, C-terminal domain Family: Alpha subunit of glutamate synthase, C-terminal domain Phyre2 PDB 2d9a chain A Region: 53 - 67 Aligned: 15 Modelled: 15 Confidence: 12.7% Identity: 20% PDB header:transcription Chain: A: PDB Molecule:myb-related protein b; PDBTitle: solution structure of rsgi ruh-050, a myb dna-binding2 domain in mouse cdna Phyre2 PDB 1fsd chain A Region: 46 - 68 Aligned: 23 Modelled: 23 Confidence: 12.1% Identity: 17% PDB header:novel sequence Chain: A: PDB Molecule:full sequence design 1 of beta beta alpha motif; PDBTitle: full sequence design 1 (fsd-1) of beta beta alpha motif,2 nmr, 41 structures Phyre2 PDB 1fsv chain A Region: 46 - 68 Aligned: 23 Modelled: 23 Confidence: 12.1% Identity: 17% PDB header:beta beta alpha motif Chain: A: PDB Molecule:full sequence design 1 of beta beta alpha motif; PDBTitle: full sequence design 1 (fsd-1) of beta beta alpha motif,2 nmr, minimized average structure Phyre2 PDB 2llk chain A Region: 53 - 67 Aligned: 15 Modelled: 15 Confidence: 10.3% Identity: 27% PDB header:cell cycle, transcription Chain: A: PDB Molecule:cyclin-d-binding myb-like transcription factor 1; PDBTitle: solution nmr structure of the n-terminal myb-like 1 domain of the2 human cyclin-d-binding transcription factor 1 (hdmp1), northeast3 structural genomics consortium (nesg) target id hr8011a Phyre2 PDB 1chk chain A Region: 51 - 66 Aligned: 16 Modelled: 16 Confidence: 10.2% Identity: 13% Fold: Lysozyme-like Superfamily: Lysozyme-like Family: Chitosanase Phyre2 PDB 1gv2 chain A domain 2 Region: 53 - 67 Aligned: 15 Modelled: 15 Confidence: 9.9% Identity: 20% Fold: DNA/RNA-binding 3-helical bundle Superfamily: Homeodomain-like Family: Myb/SANT domain Phyre2 PDB 1w0t chain A Region: 53 - 67 Aligned: 15 Modelled: 15 Confidence: 9.7% Identity: 13% Fold: DNA/RNA-binding 3-helical bundle Superfamily: Homeodomain-like Family: DNA-binding domain of telomeric protein Phyre2 PDB 1a5j chain A domain 1 Region: 53 - 67 Aligned: 15 Modelled: 15 Confidence: 9.5% Identity: 20% Fold: DNA/RNA-binding 3-helical bundle Superfamily: Homeodomain-like Family: Myb/SANT domain Phyre2 PDB 1zrr chain A domain 1 Region: 45 - 67 Aligned: 23 Modelled: 23 Confidence: 9.5% Identity: 35% Fold: Double-stranded beta-helix Superfamily: RmlC-like cupins Family: Acireductone dioxygenase Phyre2 PDB 2caz chain F Region: 51 - 64 Aligned: 14 Modelled: 14 Confidence: 9.1% Identity: 21% PDB header:protein transport Chain: F: PDB Molecule:protein srn2; PDBTitle: escrt-i core Phyre2 PDB 1q0w chain A Region: 55 - 65 Aligned: 11 Modelled: 11 Confidence: 8.2% Identity: 64% PDB header:transport protein Chain: A: PDB Molecule:vacuolar protein sorting-associated protein PDBTitle: solution structure of vps27 amino-terminal uim-ubiquitin2 complex Phyre2 PDB 1h8a chain C domain 1 Region: 53 - 67 Aligned: 15 Modelled: 15 Confidence: 7.7% Identity: 20% Fold: DNA/RNA-binding 3-helical bundle Superfamily: Homeodomain-like Family: Myb/SANT domain Phyre2 PDB 3gh1 chain A Region: 18 - 68 Aligned: 48 Modelled: 51 Confidence: 7.7% Identity: 13% PDB header:structural genomics, unknown function Chain: A: PDB Molecule:predicted nucleotide-binding protein; PDBTitle: crystal structure of predicted nucleotide-binding protein from vibrio2 cholerae Phyre2 1 d1ej5a_ 2 c2p22C_ 3 c2k42A_ 4 d1gvda_ 5 d1guua_ 6 c1guuA_ 7 d1ea0a1 8 c2d9aA_ 9 c1fsdA_ 10 c1fsvA_ 11 c2llkA_ 12 d1chka_ 13 d1gv2a2 14 d1w0ta_ 15 d1a5ja1 16 d1zrra1 17 c2cazF_ 18 c1q0wA_ 19 d1h8ac1 20 c3gh1A_ 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36

Binding site prediction

Due to computational demand, binding site predictions are not run for batch jobs

If you want to predict binding sites, please manually submit your model of choice to 3DLigandSite

Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
If you use the binding site predictions from 3DLigandSite, please also cite:
3DLigandSite: predicting ligand-binding sites using similar structures.
Wass MN, Kelley LA and Sternberg MJ Nucleic Acids Research 38, W469-73 (2010) [PubMed]
© Structural Bioinformatics Group Imperial College London Lawrence Kelley, Benjamin Jefferys Disclaimer Terms and Conditions	Component software Template detection: HHpred 1.51 Secondary structure prediction: Psi-pred 2.5 Disorder prediction: Disopred 2.4 Transmembrane prediction: Memsat_SVM Multi-template modelling and ab initio: Poing 1.0