Secondary structure and disorder prediction

		1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	I	S	S	L	W	I	A	K	T	G	L	D	A	Q	Q	T	N	M	D	V	I	A	N	N	L	A	N	V	S	T	N	G	F	K	R	Q	R	A	V	F	E	D	L	L	Y	Q	T	I	R	Q	P	G	A	Q	S	S	E	Q	T
Secondary structure
SS confidence
Disorder		?	?	?															?	?																	?				?									?	?	?	?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		T	L	P	S	G	L	Q	I	G	T	G	V	R	P	V	A	T	E	R	L	H	S	Q	G	N	L	S	Q	T	N	N	S	K	D	V	A	I	K	G	Q	G	F	F	Q	V	M	L	P	D	G	S	S	A	Y	T	R	D	G	S	F
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?	?	?	?		?		?	?	?	?	?	?	?							?		?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		Q	V	D	Q	N	G	Q	L	V	T	A	G	G	F	Q	V	Q	P	A	I	T	I	P	A	N	A	L	S	I	T	I	G	R	D	G	V	V	S	V	T	Q	Q	G	Q	A	A	P	V	Q	V	G	Q	L	N	L	T	T	F	M	N
Secondary structure
SS confidence
Disorder																						?	?	?	?			?	?	?	?	?	?	?	?	?	?			?	?	?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		D	T	G	L	E	S	I	G	E	N	L	Y	T	E	T	Q	S	S	G	A	P	N	E	S	T	P	G	L	N	G	A	G	L	L	Y	Q	G	Y	V	E	T	S	N	V	N	V	A	E	E	L	V	N	M	I	Q	V	Q	R	A	Y
Secondary structure
SS confidence
Disorder						?	?	?	?	?	?	?		?	?	?	?	?		?	?			?	?	?	?	?	?	?	?	?	?			?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260
Sequence		E	I	N	S	K	A	V	S	T	T	D	Q	M	L	Q	K	L	T	Q	L
Secondary structure
SS confidence
Disorder																				?	?
Disorder confidence

Confidence Key
High(9)											Low (0)

?	Disordered
	Alpha helix
	Beta strand

Domain analysis

Hover over an aligned region to see model and summary info

Please note, only up to the top 20 hits are modelled to reduce computer load

Rank Aligned region

PDB 3a69 chain A Region: 4 - 260 Aligned: 184 Modelled: 191 Confidence: 100.0% Identity: 51% PDB header:motor protein Chain: A: PDB Molecule:flagellar hook protein flge; PDBTitle: atomic model of the bacterial flagellar hook based on2 docking an x-ray derived structure and terminal two alpha-3 helices into an 7.1 angstrom resolution cryoem map Phyre2 PDB 1wlg chain A Region: 93 - 222 Aligned: 130 Modelled: 130 Confidence: 100.0% Identity: 46% Fold: Flagellar hook protein flgE Superfamily: Flagellar hook protein flgE Family: Flagellar hook protein flgE Phyre2 PDB 1ucu chain A Region: 223 - 260 Aligned: 38 Modelled: 38 Confidence: 85.1% Identity: 21% Fold: Phase 1 flagellin Superfamily: Phase 1 flagellin Family: Phase 1 flagellin Phyre2 PDB 1jzd chain A Region: 100 - 182 Aligned: 73 Modelled: 83 Confidence: 49.0% Identity: 19% PDB header:oxidoreductase Chain: A: PDB Molecule:thiol:disulfide interchange protein dsbc; PDBTitle: dsbc-dsbdalpha complex Phyre2 PDB 1ory chain B Region: 223 - 260 Aligned: 38 Modelled: 38 Confidence: 35.5% Identity: 21% PDB header:chaperone Chain: B: PDB Molecule:flagellin; PDBTitle: flagellar export chaperone in complex with its cognate binding partner Phyre2 PDB 1zxu chain A Region: 103 - 137 Aligned: 35 Modelled: 35 Confidence: 28.2% Identity: 17% PDB header:structural genomics, unknown function Chain: A: PDB Molecule:at5g01750 protein; PDBTitle: x-ray structure of protein from arabidopsis thaliana2 at5g01750 Phyre2 PDB 2q4m chain A domain 1 Region: 103 - 137 Aligned: 35 Modelled: 35 Confidence: 28.2% Identity: 17% Fold: Tubby C-terminal domain-like Superfamily: Tubby C-terminal domain-like Family: At5g01750-like Phyre2 PDB 1t3b chain A Region: 100 - 182 Aligned: 73 Modelled: 83 Confidence: 28.1% Identity: 15% PDB header:isomerase Chain: A: PDB Molecule:thiol:disulfide interchange protein dsbc; PDBTitle: x-ray structure of dsbc from haemophilus influenzae Phyre2 PDB 3k8v chain B Region: 223 - 244 Aligned: 22 Modelled: 22 Confidence: 27.6% Identity: 18% PDB header:structural protein Chain: B: PDB Molecule:flagellin homolog; PDBTitle: crysatl structure of a bacterial cell-surface flagellin n20c20 Phyre2 PDB 1nig chain A Region: 220 - 236 Aligned: 17 Modelled: 17 Confidence: 17.9% Identity: 24% Fold: Ferritin-like Superfamily: Cobalamin adenosyltransferase-like Family: Hypothetical protein Ta1238 Phyre2 PDB 1lvo chain A Region: 25 - 35 Aligned: 11 Modelled: 11 Confidence: 15.8% Identity: 36% Fold: Trypsin-like serine proteases Superfamily: Trypsin-like serine proteases Family: Viral cysteine protease of trypsin fold Phyre2 PDB 2q6f chain B Region: 25 - 35 Aligned: 11 Modelled: 11 Confidence: 15.2% Identity: 36% PDB header:hydrolase Chain: B: PDB Molecule:infectious bronchitis virus (ibv) main protease; PDBTitle: crystal structure of infectious bronchitis virus (ibv) main2 protease in complex with a michael acceptor inhibitor n3 Phyre2 PDB 3d23 chain A Region: 25 - 35 Aligned: 11 Modelled: 11 Confidence: 12.6% Identity: 27% PDB header:hydrolase Chain: A: PDB Molecule:3c-like proteinase; PDBTitle: main protease of hcov-hku1 Phyre2 PDB 2duc chain A domain 1 Region: 25 - 35 Aligned: 11 Modelled: 11 Confidence: 12.6% Identity: 27% Fold: Trypsin-like serine proteases Superfamily: Trypsin-like serine proteases Family: Viral cysteine protease of trypsin fold Phyre2 PDB 3kgl chain B Region: 74 - 129 Aligned: 56 Modelled: 56 Confidence: 10.6% Identity: 20% PDB header:plant protein Chain: B: PDB Molecule:cruciferin; PDBTitle: crystal structure of procruciferin, 11s globulin from2 brassica napus Phyre2 PDB 1t3b chain A domain 2 Region: 100 - 125 Aligned: 26 Modelled: 26 Confidence: 10.1% Identity: 15% Fold: Cystatin-like Superfamily: DsbC/DsbG N-terminal domain-like Family: DsbC/DsbG N-terminal domain-like Phyre2 PDB 1p9s chain A Region: 25 - 35 Aligned: 11 Modelled: 11 Confidence: 10.1% Identity: 18% Fold: Trypsin-like serine proteases Superfamily: Trypsin-like serine proteases Family: Viral cysteine protease of trypsin fold Phyre2 PDB 2v4j chain C domain 1 Region: 114 - 132 Aligned: 19 Modelled: 19 Confidence: 9.8% Identity: 42% Fold: DsrC, the gamma subunit of dissimilatory sulfite reductase Superfamily: DsrC, the gamma subunit of dissimilatory sulfite reductase Family: DsrC, the gamma subunit of dissimilatory sulfite reductase Phyre2 PDB 2oqk chain A Region: 95 - 116 Aligned: 22 Modelled: 22 Confidence: 9.3% Identity: 18% PDB header:translation Chain: A: PDB Molecule:putative translation initiation factor eif-1a; PDBTitle: crystal structure of putative cryptosporidium parvum translation2 initiation factor eif-1a Phyre2 PDB 1eej chain A domain 2 Region: 100 - 125 Aligned: 26 Modelled: 26 Confidence: 8.3% Identity: 23% Fold: Cystatin-like Superfamily: DsbC/DsbG N-terminal domain-like Family: DsbC/DsbG N-terminal domain-like Phyre2 1 c3a69A_ 2 d1wlga_ 3 d1ucua_ 4 c1jzdA_ 5 c1oryB_ 6 c1zxuA_ 7 d2q4ma1 8 c1t3bA_ 9 c3k8vB_ 10 d1niga_ 11 d1lvoa_ 12 c2q6fB_ 13 c3d23A_ 14 d2duca1 15 c3kglB_ 16 d1t3ba2 17 d1p9sa_ 18 d2v4jc1 19 c2oqkA_ 20 d1eeja2 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35

Detailed template information

# Template Alignment Coverage 3D Model Confidence % i.d. Template Information

1 c3a69A_ 100.0 51 PDB header:motor protein Chain: A: PDB Molecule:flagellar hook protein flge; PDBTitle: atomic model of the bacterial flagellar hook based on2 docking an x-ray derived structure and terminal two alpha-3 helices into an 7.1 angstrom resolution cryoem map 2 d1wlga_ 100.0 46 Fold:Flagellar hook protein flgE Superfamily:Flagellar hook protein flgE Family:Flagellar hook protein flgE 3 d1ucua_ 85.1 21 Fold:Phase 1 flagellin Superfamily:Phase 1 flagellin Family:Phase 1 flagellin 4 c1jzdA_ 49.0 19 PDB header:oxidoreductase Chain: A: PDB Molecule:thiol:disulfide interchange protein dsbc; PDBTitle: dsbc-dsbdalpha complex 5 c1oryB_ 35.5 21 PDB header:chaperone Chain: B: PDB Molecule:flagellin; PDBTitle: flagellar export chaperone in complex with its cognate binding partner 6 c1zxuA_ 28.2 17 PDB header:structural genomics, unknown function Chain: A: PDB Molecule:at5g01750 protein; PDBTitle: x-ray structure of protein from arabidopsis thaliana2 at5g01750 7 d2q4ma1 28.2 17 Fold:Tubby C-terminal domain-like Superfamily:Tubby C-terminal domain-like Family:At5g01750-like 8 c1t3bA_ 28.1 15 PDB header:isomerase Chain: A: PDB Molecule:thiol:disulfide interchange protein dsbc; PDBTitle: x-ray structure of dsbc from haemophilus influenzae 9 c3k8vB_ 27.6 18 PDB header:structural protein Chain: B: PDB Molecule:flagellin homolog; PDBTitle: crysatl structure of a bacterial cell-surface flagellin n20c20 10 d1niga_ 17.9 24 Fold:Ferritin-like Superfamily:Cobalamin adenosyltransferase-like Family:Hypothetical protein Ta1238 11 d1lvoa_ 15.8 36 Fold:Trypsin-like serine proteases Superfamily:Trypsin-like serine proteases Family:Viral cysteine protease of trypsin fold 12 c2q6fB_ 15.2 36 PDB header:hydrolase Chain: B: PDB Molecule:infectious bronchitis virus (ibv) main protease; PDBTitle: crystal structure of infectious bronchitis virus (ibv) main2 protease in complex with a michael acceptor inhibitor n3 13 c3d23A_ 12.6 27 PDB header:hydrolase Chain: A: PDB Molecule:3c-like proteinase; PDBTitle: main protease of hcov-hku1 14 d2duca1 12.6 27 Fold:Trypsin-like serine proteases Superfamily:Trypsin-like serine proteases Family:Viral cysteine protease of trypsin fold 15 c3kglB_ 10.6 20 PDB header:plant protein Chain: B: PDB Molecule:cruciferin; PDBTitle: crystal structure of procruciferin, 11s globulin from2 brassica napus 16 d1t3ba2 10.1 15 Fold:Cystatin-like Superfamily:DsbC/DsbG N-terminal domain-like Family:DsbC/DsbG N-terminal domain-like 17 d1p9sa_ 10.1 18 Fold:Trypsin-like serine proteases Superfamily:Trypsin-like serine proteases Family:Viral cysteine protease of trypsin fold 18 d2v4jc1 9.8 42 Fold:DsrC, the gamma subunit of dissimilatory sulfite reductase Superfamily:DsrC, the gamma subunit of dissimilatory sulfite reductase Family:DsrC, the gamma subunit of dissimilatory sulfite reductase 19 c2oqkA_ 9.3 18 PDB header:translation Chain: A: PDB Molecule:putative translation initiation factor eif-1a; PDBTitle: crystal structure of putative cryptosporidium parvum translation2 initiation factor eif-1a 20 d1eeja2 8.3 23 Fold:Cystatin-like Superfamily:DsbC/DsbG N-terminal domain-like Family:DsbC/DsbG N-terminal domain-like 21 d1ji8a_ not modelled 7.9 21 Fold:DsrC, the gamma subunit of dissimilatory sulfite reductase Superfamily:DsrC, the gamma subunit of dissimilatory sulfite reductase Family:DsrC, the gamma subunit of dissimilatory sulfite reductase 22 d1b2pa_ not modelled 7.8 12 Fold:beta-Prism II Superfamily:alpha-D-mannose-specific plant lectins Family:alpha-D-mannose-specific plant lectins 23 c2iyjA_ not modelled 7.8 23 PDB header:isomerase Chain: A: PDB Molecule:thiol disulfide interchange protein dsbc; PDBTitle: crystal structure of the n-terminal dimer domain of e.coli2 dsbc 24 c2dgyA_ not modelled 7.8 14 PDB header:translation Chain: A: PDB Molecule:mgc11102 protein; PDBTitle: solution structure of the eukaryotic initiation factor 1a2 in mgc11102 protein 25 d2czra1 not modelled 7.4 32 Fold:Restriction endonuclease-like Superfamily:TBP-interacting protein-like Family:TBP-interacting protein-like 26 c3bk2A_ not modelled 7.2 26 PDB header:hydrolase Chain: A: PDB Molecule:metal dependent hydrolase; PDBTitle: crystal structure analysis of the rnase j/ump complex 27 c2cazF_ not modelled 6.2 9 PDB header:protein transport Chain: F: PDB Molecule:protein srn2; PDBTitle: escrt-i core 28 c1fxzC_ not modelled 6.0 18 PDB header:plant protein Chain: C: PDB Molecule:glycinin g1; PDBTitle: crystal structure of soybean proglycinin a1ab1b homotrimer 29 c2a5wC_ not modelled 6.0 19 PDB header:oxidoreductase Chain: C: PDB Molecule:sulfite reductase, desulfoviridin-type subunit gamma PDBTitle: crystal structure of the oxidized gamma-subunit of the dissimilatory2 sulfite reductase (dsrc) from archaeoglobus fulgidus 30 c3kscD_ not modelled 5.9 20 PDB header:plant protein Chain: D: PDB Molecule:lega class; PDBTitle: crystal structure of pea prolegumin, an 11s seed globulin2 from pisum sativum l. 31 d2enga_ not modelled 5.9 35 Fold:Double psi beta-barrel Superfamily:Barwin-like endoglucanases Family:Eng V-like 32 d2c5ra1 not modelled 5.5 33 Fold:Phage replication organizer domain Superfamily:Phage replication organizer domain Family:Phage replication organizer domain 33 d1fxza2 not modelled 5.5 19 Fold:Double-stranded beta-helix Superfamily:RmlC-like cupins Family:Germin/Seed storage 7S protein 34 c1yx3A_ not modelled 5.3 36 PDB header:structural genomics, unknown function Chain: A: PDB Molecule:hypothetical protein dsrc; PDBTitle: nmr structure of allochromatium vinosum dsrc: northeast2 structural genomics consortium target op4 35 c2kjzA_ not modelled 5.2 29 PDB header:unknown function Chain: A: PDB Molecule:atc0852; PDBTitle: solution nmr structure of protein atc0852 from agrobacterium2 tumefaciens. northeast structural genomics consortium (nesg) target3 att2.

Binding site prediction

Due to computational demand, binding site predictions are not run for batch jobs

If you want to predict binding sites, please manually submit your model of choice to 3DLigandSite

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Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
If you use the binding site predictions from 3DLigandSite, please also cite:
3DLigandSite: predicting ligand-binding sites using similar structures.
Wass MN, Kelley LA and Sternberg MJ Nucleic Acids Research 38, W469-73 (2010) [PubMed]
© Structural Bioinformatics Group Imperial College London Lawrence Kelley, Benjamin Jefferys Disclaimer Terms and Conditions	Component software Template detection: HHpred 1.51 Secondary structure prediction: Psi-pred 2.5 Disorder prediction: Disopred 2.4 Transmembrane prediction: Memsat_SVM Multi-template modelling and ab initio: Poing 1.0