Secondary structure and disorder prediction

		1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	K	R	R	L	W	L	L	M	L	F	L	F	A	G	H	V	P	A	A	S	A	D	S	A	C	E	G	R	F	V	N	P	I	T	D	I	C	W	S	C	I	F	P	L	S	L	G	S	I	K	V	S	Q	G	K	V	P	D	T
Secondary structure
SS confidence
Disorder		?	?	?												?	?	?	?	?	?	?	?	?	?	?	?	?																					?		?		?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		A	N	P	S	M	P	I	Q	I	C	P	A	P	P	P	L	F	R	R	I	G	L	A	I	G	Y	W	E	P	M	A	L	T	D	V	T	R	S	P	G	C	M	V	N	L	G	F	S	L	P	A	F	G	K	T	A	Q	G	T	A
Secondary structure
SS confidence
Disorder		?	?	?	?										?	?	?				?																							?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		K	K	D	E	K	Q	V	N	G	A	F	Y	H	V	H	W	Y	K	Y	P	L	T	Y	W	L	N	I	I	T	S	L	G	C	L	E	G	G	D	M	D	I	A	Y	L	S	E	I	D	P	T	W	T	D	S	S	L	T	T	I	L
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?	?	?	?	?																							?
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		N	P	E	A	V	I	F	A	N	P	I	A	Q	G	A	C	A	A	D	A	I	A	S	A	F	N	M	P	L	D	V	L	F	W	C	A	G	S	Q	G	S	M	Y	P	F	N	G	W	V	S	N	E	S	S	P	L	Q	S	S	L
Secondary structure
SS confidence
Disorder						?	?																			?	?	?	?																						?	?	?	?	?				?
Disorder confidence
		.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300
Sequence		L	V	S	E	R	M	A	F	K	L	H	R	Q	G	M	I	M	E	T	I	G	K	N	N	A	V	C	N	E	Y	P	S	P	I	L	P	K	E	R	W	R	Y	Q	M	V	N	M	Y	P	D	S	G	Q	C	H	P	F	G	R	S
Secondary structure
SS confidence
Disorder																						?	?		?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	310	.	.	.	.	.	.	.	.	.	320	.	.	.	.	.	.	.	.	.	330
Sequence		V	T	R	W	E	T	G	K	N	P	P	N	T	K	K	N	F	G	Y	L	M	W	R	K	R	N	C	V	F	L
Secondary structure
SS confidence
Disorder				?	?							?																			?
Disorder confidence

Confidence Key
High(9)											Low (0)

?	Disordered
	Alpha helix
	Beta strand

Domain analysis

Hover over an aligned region to see model and summary info

Please note, only up to the top 20 hits are modelled to reduce computer load

Rank Aligned region

PDB 3qr3 chain B Region: 213 - 244 Aligned: 32 Modelled: 32 Confidence: 20.9% Identity: 28% PDB header:hydrolase Chain: B: PDB Molecule:endoglucanase eg-ii; PDBTitle: crystal structure of cel5a (eg2) from hypocrea jecorina (trichoderma2 reesei) Phyre2 PDB 3ifu chain A Region: 221 - 271 Aligned: 47 Modelled: 51 Confidence: 19.7% Identity: 23% PDB header:transcription Chain: A: PDB Molecule:non-structural protein; PDBTitle: the crystal structure of porcine reproductive and2 respiratory syndrome virus (prrsv) leader protease nsp1 Phyre2 PDB 2pnm chain A Region: 299 - 320 Aligned: 22 Modelled: 22 Confidence: 18.2% Identity: 23% PDB header:hydrolase Chain: A: PDB Molecule:protease vp4; PDBTitle: crystal structure of vp4 protease from infectious pancreatic necrosis2 virus (ipnv) in space group p6122 Phyre2 PDB 2go8 chain A domain 1 Region: 80 - 88 Aligned: 9 Modelled: 9 Confidence: 12.4% Identity: 33% Fold: Ferredoxin-like Superfamily: Dimeric alpha+beta barrel Family: PG130-like Phyre2 PDB 1yvu chain A domain 1 Region: 210 - 217 Aligned: 8 Modelled: 8 Confidence: 10.2% Identity: 63% Fold: SH3-like barrel Superfamily: PAZ domain Family: PAZ domain Phyre2 PDB 3bmz chain A Region: 84 - 101 Aligned: 18 Modelled: 18 Confidence: 9.0% Identity: 39% PDB header:biosynthetic protein Chain: A: PDB Molecule:putative uncharacterized protein; PDBTitle: violacein biosynthetic enzyme vioe Phyre2 PDB 1lg4 chain A Region: 112 - 140 Aligned: 29 Modelled: 29 Confidence: 8.7% Identity: 21% Fold: Prion-like Superfamily: Prion-like Family: Prion-like Phyre2 PDB 1rut chain X domain 1 Region: 215 - 225 Aligned: 11 Modelled: 11 Confidence: 8.7% Identity: 36% Fold: Glucocorticoid receptor-like (DNA-binding domain) Superfamily: Glucocorticoid receptor-like (DNA-binding domain) Family: LIM domain Phyre2 PDB 1w62 chain B Region: 241 - 294 Aligned: 54 Modelled: 54 Confidence: 8.2% Identity: 17% PDB header:racemase Chain: B: PDB Molecule:b-cell mitogen; PDBTitle: proline racemase in complex with one molecule of pyrrole-2-2 carboxylic acid (hemi form) Phyre2 PDB 1i17 chain A Region: 112 - 140 Aligned: 29 Modelled: 29 Confidence: 7.4% Identity: 21% Fold: Prion-like Superfamily: Prion-like Family: Prion-like Phyre2 PDB 1skz chain A domain 1 Region: 23 - 38 Aligned: 16 Modelled: 16 Confidence: 7.4% Identity: 50% Fold: Knottins (small inhibitors, toxins, lectins) Superfamily: Leech antihemostatic proteins Family: Huristasin-like Phyre2 PDB 1v47 chain A domain 1 Region: 220 - 233 Aligned: 14 Modelled: 14 Confidence: 7.4% Identity: 29% Fold: PUA domain-like Superfamily: PUA domain-like Family: ATP sulfurylase N-terminal domain Phyre2 PDB 1j2o chain A domain 1 Region: 215 - 222 Aligned: 8 Modelled: 8 Confidence: 7.3% Identity: 38% Fold: Glucocorticoid receptor-like (DNA-binding domain) Superfamily: Glucocorticoid receptor-like (DNA-binding domain) Family: LIM domain Phyre2 PDB 2p0v chain A Region: 277 - 308 Aligned: 32 Modelled: 32 Confidence: 7.1% Identity: 16% PDB header:structural genomics, unknown function Chain: A: PDB Molecule:hypothetical protein bt3781; PDBTitle: crystal structure of bt3781 protein from bacteroides2 thetaiotaomicron, northeast structural genomics target3 btr58 Phyre2 PDB 2p0v chain A domain 1 Region: 277 - 308 Aligned: 32 Modelled: 32 Confidence: 7.1% Identity: 16% Fold: alpha/alpha toroid Superfamily: Six-hairpin glycosidases Family: CPF0428-like Phyre2 PDB 1x6v chain A domain 1 Region: 220 - 233 Aligned: 14 Modelled: 14 Confidence: 7.0% Identity: 29% Fold: PUA domain-like Superfamily: PUA domain-like Family: ATP sulfurylase N-terminal domain Phyre2 PDB 1l5j chain A domain 3 Region: 95 - 142 Aligned: 47 Modelled: 48 Confidence: 6.9% Identity: 21% Fold: Aconitase iron-sulfur domain Superfamily: Aconitase iron-sulfur domain Family: Aconitase iron-sulfur domain Phyre2 PDB 2jb1 chain B Region: 208 - 247 Aligned: 38 Modelled: 40 Confidence: 6.9% Identity: 24% PDB header:oxidoreductase Chain: B: PDB Molecule:l-amino acid oxidase; PDBTitle: the l-amino acid oxidase from rhodococcus opacus in complex2 with l-alanine Phyre2 PDB 1g8f chain A domain 1 Region: 220 - 233 Aligned: 14 Modelled: 14 Confidence: 6.7% Identity: 21% Fold: PUA domain-like Superfamily: PUA domain-like Family: ATP sulfurylase N-terminal domain Phyre2 PDB 1jl0 chain A Region: 135 - 169 Aligned: 27 Modelled: 35 Confidence: 6.7% Identity: 33% Fold: S-adenosylmethionine decarboxylase Superfamily: S-adenosylmethionine decarboxylase Family: S-adenosylmethionine decarboxylase Phyre2 1 c3qr3B_ 2 c3ifuA_ 3 c2pnmA_ 4 d2go8a1 5 d1yvua1 6 c3bmzA_ 7 d1lg4a_ 8 d1rutx1 9 c1w62B_ 10 d1i17a_ 11 d1skza1 12 d1v47a1 13 d1j2oa1 14 c2p0vA_ 15 d2p0va1 16 d1x6va1 17 d1l5ja3 18 c2jb1B_ 19 d1g8fa1 20 d1jl0a_ 21 22 23 24 25 26 27 28 29 30 31 32

Detailed template information

# Template Alignment Coverage 3D Model Confidence % i.d. Template Information

1 c3qr3B_ 20.9 28 PDB header:hydrolase Chain: B: PDB Molecule:endoglucanase eg-ii; PDBTitle: crystal structure of cel5a (eg2) from hypocrea jecorina (trichoderma2 reesei) 2 c3ifuA_ 19.7 23 PDB header:transcription Chain: A: PDB Molecule:non-structural protein; PDBTitle: the crystal structure of porcine reproductive and2 respiratory syndrome virus (prrsv) leader protease nsp1 3 c2pnmA_ 18.2 23 PDB header:hydrolase Chain: A: PDB Molecule:protease vp4; PDBTitle: crystal structure of vp4 protease from infectious pancreatic necrosis2 virus (ipnv) in space group p6122 4 d2go8a1 12.4 33 Fold:Ferredoxin-like Superfamily:Dimeric alpha+beta barrel Family:PG130-like 5 d1yvua1 10.2 63 Fold:SH3-like barrel Superfamily:PAZ domain Family:PAZ domain 6 c3bmzA_ 9.0 39 PDB header:biosynthetic protein Chain: A: PDB Molecule:putative uncharacterized protein; PDBTitle: violacein biosynthetic enzyme vioe 7 d1lg4a_ 8.7 21 Fold:Prion-like Superfamily:Prion-like Family:Prion-like 8 d1rutx1 8.7 36 Fold:Glucocorticoid receptor-like (DNA-binding domain) Superfamily:Glucocorticoid receptor-like (DNA-binding domain) Family:LIM domain 9 c1w62B_ 8.2 17 PDB header:racemase Chain: B: PDB Molecule:b-cell mitogen; PDBTitle: proline racemase in complex with one molecule of pyrrole-2-2 carboxylic acid (hemi form) 10 d1i17a_ 7.4 21 Fold:Prion-like Superfamily:Prion-like Family:Prion-like 11 d1skza1 7.4 50 Fold:Knottins (small inhibitors, toxins, lectins) Superfamily:Leech antihemostatic proteins Family:Huristasin-like 12 d1v47a1 7.4 29 Fold:PUA domain-like Superfamily:PUA domain-like Family:ATP sulfurylase N-terminal domain 13 d1j2oa1 7.3 38 Fold:Glucocorticoid receptor-like (DNA-binding domain) Superfamily:Glucocorticoid receptor-like (DNA-binding domain) Family:LIM domain 14 c2p0vA_ 7.1 16 PDB header:structural genomics, unknown function Chain: A: PDB Molecule:hypothetical protein bt3781; PDBTitle: crystal structure of bt3781 protein from bacteroides2 thetaiotaomicron, northeast structural genomics target3 btr58 15 d2p0va1 7.1 16 Fold:alpha/alpha toroid Superfamily:Six-hairpin glycosidases Family:CPF0428-like 16 d1x6va1 7.0 29 Fold:PUA domain-like Superfamily:PUA domain-like Family:ATP sulfurylase N-terminal domain 17 d1l5ja3 6.9 21 Fold:Aconitase iron-sulfur domain Superfamily:Aconitase iron-sulfur domain Family:Aconitase iron-sulfur domain 18 c2jb1B_ 6.9 24 PDB header:oxidoreductase Chain: B: PDB Molecule:l-amino acid oxidase; PDBTitle: the l-amino acid oxidase from rhodococcus opacus in complex2 with l-alanine 19 d1g8fa1 6.7 21 Fold:PUA domain-like Superfamily:PUA domain-like Family:ATP sulfurylase N-terminal domain 20 d1jl0a_ 6.7 33 Fold:S-adenosylmethionine decarboxylase Superfamily:S-adenosylmethionine decarboxylase Family:S-adenosylmethionine decarboxylase 21 c2zkrg_ not modelled 6.6 36 PDB header:ribosomal protein/rna Chain: G: PDB Molecule:rna expansion segment es9; PDBTitle: structure of a mammalian ribosomal 60s subunit within an2 80s complex obtained by docking homology models of the rna3 and proteins into an 8.7 a cryo-em map 22 d1zata2 not modelled 6.2 0 Fold:L,D-transpeptidase pre-catalytic domain-like Superfamily:L,D-transpeptidase pre-catalytic domain-like Family:L,D-transpeptidase pre-catalytic domain-like 23 d1m8pa1 not modelled 6.1 21 Fold:PUA domain-like Superfamily:PUA domain-like Family:ATP sulfurylase N-terminal domain 24 c2hnhA_ not modelled 6.1 35 PDB header:transferase Chain: A: PDB Molecule:dna polymerase iii alpha subunit; PDBTitle: crystal structure of the catalytic alpha subunit of e. coli2 replicative dna polymerase iii 25 c2ezvA_ not modelled 6.0 18 PDB header:hydrolase/dna Chain: A: PDB Molecule:type ii restriction enzyme sfii; PDBTitle: crystal structure of tetrameric restriction endonuclease2 sfii bound to cognate dna. 26 d1u7ka_ not modelled 6.0 50 Fold:Retrovirus capsid protein, N-terminal core domain Superfamily:Retrovirus capsid protein, N-terminal core domain Family:Retrovirus capsid protein, N-terminal core domain 27 d1yy9a4 not modelled 5.8 33 Fold:Knottins (small inhibitors, toxins, lectins) Superfamily:Growth factor receptor domain Family:Growth factor receptor domain 28 c2k2iB_ not modelled 5.6 36 PDB header:cell cycle Chain: B: PDB Molecule:sfi1 peptide; PDBTitle: nmr solution structure of the c-terminal domain (t94-y172)2 of the human centrin 2 in complex with a repeat sequence of3 human sfi1 (r641-t660) 29 d1uzka3 not modelled 5.3 42 Fold:TB module/8-cys domain Superfamily:TB module/8-cys domain Family:TB module/8-cys domain 30 d1jhda1 not modelled 5.2 21 Fold:PUA domain-like Superfamily:PUA domain-like Family:ATP sulfurylase N-terminal domain 31 d1tm0a_ not modelled 5.2 15 Fold:Diaminopimelate epimerase-like Superfamily:Diaminopimelate epimerase-like Family:Proline racemase 32 c3k4iC_ not modelled 5.0 31 PDB header:structural genomics, unknown function Chain: C: PDB Molecule:uncharacterized protein; PDBTitle: crystal structure of uncharacterized protein pspto_3204 from2 pseudomonas syringae pv. tomato str. dc3000

Binding site prediction

Due to computational demand, binding site predictions are not run for batch jobs

If you want to predict binding sites, please manually submit your model of choice to 3DLigandSite

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Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
If you use the binding site predictions from 3DLigandSite, please also cite:
3DLigandSite: predicting ligand-binding sites using similar structures.
Wass MN, Kelley LA and Sternberg MJ Nucleic Acids Research 38, W469-73 (2010) [PubMed]
© Structural Bioinformatics Group Imperial College London Lawrence Kelley, Benjamin Jefferys Disclaimer Terms and Conditions	Component software Template detection: HHpred 1.51 Secondary structure prediction: Psi-pred 2.5 Disorder prediction: Disopred 2.4 Transmembrane prediction: Memsat_SVM Multi-template modelling and ab initio: Poing 1.0