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Job DescriptionP08956
Confidence100.00%DateThu Jan 5 11:01:43 GMT 2012
Rank3Aligned Residues609
% Identity16%Templatec2w74B_
PDB info PDB header:hydrolaseChain: B: PDB Molecule:type i restriction enzyme ecor124ii r protein; PDBTitle: mutant (k220r) of the hsdr subunit of the ecor124i2 restriction enzyme in complex with atp
Resolution2.74 Å

  Insertion relative to template
  Deletion relative to template
  Catalytic residue from the CSA
 
Detailed help on interpreting your alignment


   163......170.........180.........190.........200.........210.........220.........230.........240..
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Query Sequence  QTQAEVEAQQQKLVALNGYIAILEGKQQETEAQTQARLAALEAQLAEKNAELAKQTEQERKAYHKEITDQAIKRTLNLSE
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Template Conservation    
   
   
  
   
           
       
   
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Template Sequence  QSESDLERELIQDLRNQGYEFISVKSQSAMLANVREQLQNLNGVV. . . . . . . . . . . . FNDSEWRRFTEQYLDNPSDGILD
Template Known Secondary structure  SSTT




ST

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TT

TT

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   31........40.........50.........60.........70..... ....80.........90........
 
   243......250.........260.........270.........280.........290.........300.........310.........320..
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Query Sequence  EESRFLIDAQLRKAGWQADSKTLRFSKGARPEPGVNKAIAEWPTGKDETGNQGFADYVLFVGLKPIAVVEAKRNNIDVPA
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Template Sequence  KTRKIHIDYICDFIFDDERLENIYLIDKKNLMRNKVQIIQQF. . . . . . . . . . . RYDVTILVNGLPLVQIELKKRGVAIRE
Template Known Secondary structure  T


TT

S

SSSGGGS


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TT

TTS
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   99100.........110.........120.........130.........140 .........150.........160.......
 
   323......330.........340.........350.........360.........370.........380.........390.........400..
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Query Sequence  RLNESYRYSKCFDNGFLRETLLEHYSPDEVHEAVPEYETSWQDTSGKQRFKIPFCYSTNGREYRATMKTKSGIWYRDVRD
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Template Sequence  AFNQISEN. . . . . SLFKYLQLFVISNGTDTRYFAN. . . . . . . . . . . . . . . . . TTKRDRNSFDFTMNWAKSDNTLIKD. . .
Template Known Secondary structure 


S.....GGGGG


SS

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S
STT
SGGG
B

B
TT

B

S...
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   176...180... ......190.........200..... ....210.........220.........230
 
   403......410.........420.........430.........440.........450.........460... ......470...
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Query Sequence  TRNMSKALPEWHRPEELLEMLGSEPQKQNQWFADNPGMSELGLRYYQEDAVRAVEKAIVKG. . . . . . . . . QQEILLAMAT
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Template Sequence  . . . LKDFTATCFQKHTLLNVLVNYSVF. . . . . . . DSSQTLLVMRPYQIAATERILWKIKSSFTAKNWSKPESGGYIWHTT
Template Known Secondary structure  ...TTSS.......
TTS


TT

SSGGGS
T
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   240.........250.........260... ......270.........280.........290.........300.........
 
   474.....480.........490.........500.........510.........520.........530.........540.........550...
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Query Sequence  GTGKTRTAIAMMFRLIQSQRFKRILFLVDRRSLGEQALGAFEDTRINGDTFNSIFDIKGLTDKFPEDSTKIHVATVQSLV
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Template Sequence  GSGKTLTSFKAARLATELDFIDKVFFVVDRKDLDYQTMKEYQRFSPD. . SVNGSENTAGLKRNLDKDDNKIIVTTIQKLN
Template Known Secondary structure  TSSTT
TT


GGG

TTSTT..

SSS


TT
TT

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   310.........320.........330.........340.........350...... ...360.........370.........380.......
 
   554.....560.........570.........580.........590.........600.........610.........620. ....
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Query Sequence  KRTLQSDEPMPVARYDCIVVDEAHRGYILDKEQTEGELQFRSQLDYVSAYRRILDHFDAVKIALTATP. . . . . . . . ALHT
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Template Sequence  NLMKAESDLPVYNQQVVFIFDECHRSQFGEAQKNLKKKFK. . . . . . . . . . . . . . . . . RYYQFGFTGTPIFPENALGSETT
Template Known Secondary structure 
S

GGGGSS


S.................SS


GGG
TTS

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   388.390.........400.........410.........420....... ..430.........440.........450
 
   626...630.........640.........650.........660.........670.........680.........690.........700.....
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Query Sequence  VQIFGEPVYRYTYRTAVIDGFLIDQDPPIQIITRNAQEGVYLSKGEQVERISPQGEVINDTLEDDQDFEVADFNRGLVIP
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Template Sequence  ASVFGRELHSYVITDAIRDEKVL. . . . KFKVDYNDVRPQFKSLETERIQEITQYILNNFRQKTHRTFPGSKGFNAMLAVS
Template Known Secondary structure 
S
TTSS
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TTTTTTT


T

STT




S
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   451........460.........470... ......480.........490.........500.........510.........520......
 
   706...710.........720.........730.........740.........750.........760.........770.........780.....
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Query Sequence  AFNRAVCNELTNYLDPTGSQKTLVFCVTNAHADMVVEELRAAFKKKYPQLEHDAIIKITGDADKDARKVQTMITRFNKER
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Template Sequence  SVDAAKA. . . . YYATFKPLRIATIFSFAGEISDETFDTAKEFLDAAIREYNSHFKTNFSTDSNGFQNYYRDLAQRVKNQD
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T



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   546...550.. .......560.........570.........580.........590.........600.........610.........620.
 
   786...790.........800.........810.........820.........830.........840.........850.........
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Query Sequence  LPNIVVTVDLLTTGVDIPSICNIVFLRKVRSRILYEQMKGRATRLCPEVNKTSFKIFDCVDIYSTLESVDTMRP
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Template Sequence  . IDLLIVVGMFLTGFDAPTLNTLFVDKNLRYHGL. MQAFSRTNRIYDA. TKTFGNIVTFRDLERSTIDAITLFG
Template Known Secondary structure  .SSSSTTSSS


TT
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.GGG




T.T

SSS

TS
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   650.........660.........670.........680.. .......690..... ....700.........710.........720
 
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Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
 
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Lawrence Kelley, Benjamin Jefferys 
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