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Job DescriptionP33570
Confidence100.00%DateThu Jan 5 11:52:13 GMT 2012
Rank11Aligned Residues635
% Identity24%Templatec2g28A_
PDB info PDB header:oxidoreductaseChain: A: PDB Molecule:pyruvate dehydrogenase e1 component; PDBTitle: e. coli pyruvate dehydrogenase h407a variant2 phosphonolactylthiamin diphosphate complex
Resolution1.85 Å

  Insertion relative to template
  Deletion relative to template
  Catalytic residue from the CSA
 
Detailed help on interpreting your alignment


   4.....10.........20.. .......30.........40.........50.........60.........70.......
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Query Sequence  KDLANAIRALSMDAVQKAN. . . . . . SGHPGAPMGMADIAEVLWNDFLKHNPTDPTWYDRDRFILSNGHASMLLYSLLHLT
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Template Sequence  RRIRSAIRWNAIMTVLRASKKDLELGGHMASFQSSATIYDVCFNHFFR. . . . ARNEQDGGDLVYFQGHISPGVYARAFLE
Template Known Secondary structure  TT
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   7980.........90.........100.........110.........120...... ...130.........140.........150....
 
   78.80.........90.. .......100....... ..110.........120.........130.........140.........150....
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Query Sequence  GYDLPLEELKNFRQL. . HSKTPGHPEIGYTPG. VETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGD
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Template Sequence  GR. LTQEQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK. . . DTSKQTVYAFLGD
Template Known Secondary structure  TS.S
TTBT

TTSS
B
SS

TTTSTTT





STTTTS
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TT

T
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   155. ...160.........170.........180.........190.........200.........210....... ..220.........230
 
   155....160.........170.........180.........190....... ..200.........210....
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Query Sequence  GCLMEGISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFT. . DDTAKRFEAYHWHVIHE. . . . . . . . . . . . . . . . . .
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Template Sequence  GEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKIINELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKL
Template Known Secondary structure  GGGGSTTTT
TT
SB
SSSBSSTTS
TT

B
GGG

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   231........240.........250.........260.........270.........280.........290.........300.........310
 
   215....220.........230.........240....
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Query Sequence  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . IDGHDPQAVKEAILEAQSVKDKPSLIICRT
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Template Sequence  IQLMNETVDGDYQTFKSKDGAYVREHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHT
Template Known Secondary structure  S
TTS
TGGGSTTTS
T

BGGG


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   311........320.........330.........340.........350.........360.........370.........380.........390
 
   245....250.........260.........270.........280.........290.........300.........310.........320....
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Query Sequence  VIGFGSPNKAGKEEAHGAPLGEEEVALARQKLGWHHPPFEIPKEIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEF
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Template Sequence  IKGYGMGDA. . . . . . . . . . . AMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEE. . . HTYLHAQRQKLHGYLPSRQPNF
Template Known Secondary structure 
TTTT
TT
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TT
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TT






TTS...TTS
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   391........ 400.........410.........420.........430.... .....440.........450......
 
   325....330.........340.........350.........360....... ..370.........380....... ..390........
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Query Sequence  TRRMSGGLPKDWEKTTQKYINELQANPAKIATRKASQNTLNAY. . . GPMLPELLGGSADLAPSNLT. . . IWKGSVSLKED
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Template Sequence  TEKLELPSLQDFGALLEE. . . . . . . QSKEISTTIAFVRALNVMLKNKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPED
Template Known Secondary structure 
S





GGGGS
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SS
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TT
TTTTTTSS
TGGGT

BB


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   470.........480....... ..490.........500.........510.........520.........530.........540..
 
   399400.........410.........420.... .....430.........440....... ..450.........460.........470.
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Query Sequence  PAGNYIHYGVREFGMTAIANGIAHHG. . . . . GFVPYTATFLMFVEYARNAARMA. . ALMKARQIMVYTHDSIGLGEDGPT
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Template Sequence  EKGQILQEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFLIGGTSGRTTLNGEGLQ
Template Known Secondary structure  TT

B

S
TTS


GGGSTT


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STTTSTTT
TT
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   560.........570.........580.........590.........600.........610.........620.........630.........
 
   472.......480.........490.........500........ .510.........520........ .530.........540.....
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Query Sequence  HQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER. . . . HNGPTALILSRQNLAQVERT. . PDQVKEIARGGYVLKDS
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Template Sequence  HEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEKQENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETI
Template Known Secondary structure  T

S
TTSTT


SSTT





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TT
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   640.........650.........660.........670.........680.........690.........700.........710.........
 
   546...550.........560.........570... ......580.........590.........600 .........610..
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Query Sequence  . GGKPDIILIATGSEMEITLQAAEKLAGE. GRNVRVVSLPSTDIFDAQDEEYRESVL. . . . . . . . . . . PSNVAARVAVEA
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Template Sequence  EGSKGKVQLLGSGSILRHVREAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPAVA
Template Known Secondary structure 

SS
GGG
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SS
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   720.........730.........740.........750.........760.........770.........780.........790.........
 
   613......620.... .....630.........640.........650.........660.......
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Query Sequence  . . GIADYWYKYVGL. . . . . KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA
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Template Sequence  STDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGELAKRGE
Template Known Secondary structure 
SS
TTTGGG

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S



S
TT
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   800.........810.........820.........830.........840.........850.........860.
 
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Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
 
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Lawrence Kelley, Benjamin Jefferys 
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