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Job DescriptionP33570
Confidence100.00%DateThu Jan 5 11:52:13 GMT 2012
Rank8Aligned Residues650
% Identity48%Templatec1tkcA_
PDB info PDB header:transferaseChain: A: PDB Molecule:transketolase; PDBTitle: specificity of coenzyme binding in thiamin diphosphate2 dependent enzymes: crystal structures of yeast3 transketolase in complex with analogs of thiamin4 diphosphate
Resolution2.70 Å

  Insertion relative to template
  Deletion relative to template
  Catalytic residue from the CSA
 
Detailed help on interpreting your alignment


   4... ..10.........20.........30.........40.........50.........60.........70.........80..
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Query Sequence  KDLA. NAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFLKHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLP
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Template Conservation    
    

      
     

 
  

  

   
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Template Sequence  DKLAVSTIRILAVDTVSKANSGHPGAPLGMAPAAHVLWSQ. MRMNPTNPDWINRDRFVLSNGHAVALLYSMLHLTGYDLS
Template Known Secondary structure  T
S

T.



TT
TT
TT

SSGGGTTSS

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   8.10.........20.........30.........40....... ..50.........60.........70.........80......
 
   83......90.........100.........110.........120.........130.........140.........150.........160..
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Query Sequence  LEELKNFRQLHSKTPGHPEIGYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEGIS
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Template Sequence  IEDLKQFRQLGSRTPGHPEFE. LPGVEVTTGPLGQGISNAVGMAMAQANLAATYNKPGFTLSDNYTYVFLGDGCLQEGIS
Template Known Secondary structure  TTTSTT
S

SS

TT.STT

S


STT
BTTB






S
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   87..90.........100....... ..110.........120.........130.........140.........150.........160.....
 
   163......170.........180.........190.........200.........210...... ...220.........230.........240.
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Query Sequence  HEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEID. GHDPQAVKEAILEAQSVKDKPSLII
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Template Sequence  SEASSLAGHLKLGNLIAIYDDNKITIDGATSISFDEDVAKRYEAYGWEVLYVENGNEDLAGIAKAIAQAKLSKDKPTLIK
Template Known Secondary structure  TT
TT
SB
SSSB

S
T
TTTTT
TT
TTS
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   166...170.........180.........190.........200.........210.........220.........230.........240.....
 
   242.......250.........260.........270........ .280.........290...... ...300.........310........
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Query Sequence  CRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKLGW. HHPPFEIPKEIYHAWDAR. . EKGEKAQQSWNEKFAAYKKAHP
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Template Sequence  MTTTIGYGSLH. AGSHSVHGAPLKADDVKQLKSKFGFNPDKSFVVPQEVYDHYQKTILKPGVEANNKWNKLFSEYQKKFP
Template Known Secondary structure 

TTTTSTT.TTSGGGGSS


TS
TT

S


TS
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   246...250...... ...260.........270.........280.........290.........300.........310.........320....
 
   319320.........330.........340.........350.........360.........370.........380..... ....390..
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Query Sequence  QLAEEFTRRMSGGLPKDWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSN. . . . . . LTIWKGS
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Template Conservation                
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Template Sequence  ELGAELARRLSGQLPAN. . . . WESKLPTYTAKDSAVATRKLSETVLEDVYNQLPELIGGSADLTPSNLTRWKEALDFQPP
Template Known Secondary structure  T


TT....GGGGS



TTS

TT
TTSS
T


TT

BB

G
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   325....330.........340. ........350.........360.........370.........380.........390.........400
 
   393......400.........410.........420..... ....430.........440.........450.........460.........470.
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Query Sequence  VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGG. FVPYTATFLMFVEYARNAARMAALMKARQIMVYTHDSIGLGEDGPT
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Template Sequence  SSGSGNYSGRYIRYGIREHAMGAIMNGISAFGANYKPYGGTFLNFVSYAAGAVRLSALSGHPVIWVATHDSIGVGEDGPT
Template Known Secondary structure  GGSS
BTT



S

TT
T




SGGG
TT
TT
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   401........410.........420.........430.........440.........450.........460.........470.........480
 
   472.......480.........490.........500.........510.........520.........530.........540.........550.
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Query Sequence  HQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVERHNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDI
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Template Sequence  HQPIETLAHFRSLPNIQVWRPADGNEVSAAYKNSLESKHTPSIIALSRQNLPQLE. . GSSIESASKGGYVLQD. VANPDI
Template Known Secondary structure  SS
SSTTSTT



S
SS



SS


T..T

TTS

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S
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   481........490.........500.........510.........520.........530..... ....540.........550. ......
 
   552.......560.........570.........580.........590.........600.........610.........620.........630.
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Query Sequence  ILIATGSEMEITLQAAEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGLKGAIVGM
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Template Sequence  ILVATGSEVSLSVEAAKTLAAKNIKARVVSLPDFFTFDKQPLEYRLSVLPDNVP. IMSVEVLATTCWG. . . KYAHQSFGI
Template Known Secondary structure 
TTT


S
TS
S
SSS
.SS
GGGGG...GT
S
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   558.560.........570.........580.........590.........600.........610. ........620.... .....630...
 
   632.......640.........650.........660.......
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Query Sequence  TGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA
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Template Conservation 
 
   
    
    


   
   
   
   
 
Template Sequence  DRFGASGKAPEVFKFFGFTPEGVAERAQKTIAFYKG
Template Known Secondary structure 
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STT
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   634.....640.........650.........660.........
 
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Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
 
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Lawrence Kelley, Benjamin Jefferys 
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