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Job DescriptionP77488
Confidence100.00%DateThu Jan 5 12:29:48 GMT 2012
Rank5Aligned Residues596
% Identity18%Templatec2g28A_
PDB info PDB header:oxidoreductaseChain: A: PDB Molecule:pyruvate dehydrogenase e1 component; PDBTitle: e. coli pyruvate dehydrogenase h407a variant2 phosphonolactylthiamin diphosphate complex
Resolution1.85 Å

  Insertion relative to template
  Deletion relative to template
  Catalytic residue from the CSA
 
Detailed help on interpreting your alignment


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Query Sequence  PKESLPKLCDELRRYLLDSV. SRS. . . . . . SGHFASGLGTVELTVALHYVY. . . . . NTPFDQLIWDVGHQA. . . YPHKIL
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Template Sequence  NLELERRIRSAIRWNAIMTVLRASKKDLELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFL
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   74.....80.........90.........100.........110.........120.........130.........140.........150...
 
   8990. ........100. ........110..... ....120.........130......... 140.........150.....
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Query Sequence  TGR. . RDKIGTIRQK. . . GGLHPFPWRGESEY. DVLSVGHSSTSISAGIGIAVAAEK. . . . . . . EGKNRRTVCVIGDGAI
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Template Sequence  EGRLTQEQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLKDTSKQTVYAFLGDGEM
Template Known Secondary structure  TTSS
TTBT

TTSS
B
SS

TTTSTTT





STTTTS


TT

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   154.....160.........170.........180.........190.........200.........210.........220.........230...
 
   156...160.........170...... ...180......... 190.........200.........210.........220...
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Query Sequence  TAGMAFEAMNHAGDIRPDMLV. ILNDNEMSISENV. . . GALNNHLAQLLSGKLYSSLREGGKKVFSGVPPIK. . . . . . . .
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Template Sequence  DEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKIINELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQL
Template Known Secondary structure  GSTTTT
TT
SB
SSSBSSTTS
TT

B
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S
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   234.....240.........250.........260.........270.........280.........290.........300.........310...
 
   224.....230.........240.........250.........260.........270... ......280.....
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Query Sequence  . . . . . . . . . . . . . . . . . ELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLK. GPQFLHIMTKKG
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Template Sequence  MNETVDGDYQTFKSKDGAYVREHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTIKG
Template Known Secondary structure  S
TTS
TGGGSTTTS
T

BGGG


SS


TT
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   314.....320.........330.........340.........350.........360.........370.........380.........390...
 
   286...290..... ....300.........310......
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Query Sequence  RGYEPAEKDP. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ITFHAVPKFDPSSGCLPKSSG. . . . . . . . . .
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Template Sequence  YGMGDAAMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHTYLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLE
Template Known Secondary structure  TT
TT


TT
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TT






TTSTTS
SS


SS

S





GGGGS
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   394.....400.........410.........420.........430.........440.........450.........460.........470...
 
   317..320.........330.. .......340.........350........ .360.........370.........380..
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Query Sequence  . . . GLPSYSKIFGDWLCET. . . AAKDNKLMAITPAMREGSGMVEFSRK. . . . . . . . FPDRYFDVAIAEQHAVTFAAGLAI
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Template Sequence  EQSKEISTTIAFVRALNVMLKNKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPEDEKGQILQEGINELGAGCSWLAAAT
Template Known Secondary structure 

SS
B
TT
TTTTTTSS
TGGGT

BB


BTT

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   487..490.........500.........510.........520.........530.........540.........550.........560......
 
   383......390.........400..... ....410.........420... ......430.........440.........450....
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Query Sequence  . . . . . . GGYKPIVAIYSTFLQRAYDQVLH. DVAIQKLPVLFAIDRAGI. VGADGQTHQGAFDLSYLRCIPEMVIMTPSDE
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Template Sequence  SYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFLIGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYA
Template Known Secondary structure  TS


GGGSTT


S
STTTSTTT
TTT

S
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S
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   584.....590.........600.........610.........620.........630.........640.........650.........660...
 
   455....460....... ..470.........480 .........490.........500. ........510.........520...
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Query Sequence  NECRQMLYTGYHY. . . . NDGPSAVRYPRGN. . . . AVGVELTPLEKLPIGKGIVKR. . . RGEKLAILNFGTLMPEAAKVAE
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Template Sequence  YEVAVIMHDGLERMYGEKQENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVREAAE
Template Known Secondary structure  STT





S

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   664.....670.........680.........690.........700.........710.........720.........730.........740...
 
   524.... .530.........540.........550.. .......560... ......570.........580.....
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Query Sequence  SLNAT. LVDMRFVKPLDEALILEMAASHEA. . . . . . . . . . . . . . . LVTVEENAIMG. . GAGSGVNEVLMAHRKPVPVLNI
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Template Sequence  ILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPAVASTDYMKLFAEQVRTYVPADDYRVL
Template Known Secondary structure 
S

TTS






SS

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SS
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   744.....750.........760.........770.........780.........790.........800.........810.........820...
 
   586...590.........600.........610.........620
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Query Sequence  GLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA
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Template Sequence  GT. DGFGRSDSRENLRHHFEVDASYVVVAALGELA
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S
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   824. ....830.........840.........850.......
 
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Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
 
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Lawrence Kelley, Benjamin Jefferys 
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