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Job DescriptionP0AFG3
Confidence99.89%DateWed Jan 25 15:20:33 GMT 2012
Rank33Aligned Residues459
% Identity12%Templatec2g28A_
PDB info PDB header:oxidoreductaseChain: A: PDB Molecule:pyruvate dehydrogenase e1 component; PDBTitle: e. coli pyruvate dehydrogenase h407a variant2 phosphonolactylthiamin diphosphate complex
Resolution1.85 Å

  Insertion relative to template
  Deletion relative to template
  Catalytic residue from the CSA
 
Detailed help on interpreting your alignment


   322.......330.........340.. .......350.........360.........370......... 380.........390......
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Query Sequence  HLEIVSPVVIGSVRARLDRLD. . . . EPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYE. VGGTVRIVINNQVGFTT
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Template Sequence  SMGLGPIGAIYQAKFLKYLEHRGLKDTSKQTVYAFL. . GDGEMD. EPESKGAITIATREKLDNL. . . VFVINCNLQRLDG
Template Known Secondary structure  STTTTS


TT

..TGGGG.STTTT
TT...
SB
SSS
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   193......200.........210.........220........ .230.... .....240.........250... ......260......
 
   397..400.........410.........420..
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Query Sequence  SNPLDARSTPYCTDIGKMVQAPIFHV. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
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Template Sequence  PVTGNGKIINELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYVREHFFGKYPETAA
Template Known Secondary structure  BSSTTS
TT

B
GGG

S
S
TTS
TGGGS
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   267..270.........280.........290.........300.........310.........320.........330.........340......
 
   423......430.........440.........450.........460.........470.........480.......
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Query Sequence  . . . . . . . . . . . . . . . NADDPEAVAFVTRLALDFRNTFKRDVFIDLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIY
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Template Sequence  LVADWTDEQIWALNRGGHDPKKIYAAFKKA. . . QETKGKATVILAHTIKGYGMGDAAM. . . . . . DGVRHIRDRFNVP. VS
Template Known Secondary structure  TTTS
T

BGGG
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SS


TTTT
TT


......TT
S.

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   347..350.........360.........370...... ...380.........390.........400. ........410.... ..
 
   488.490.........500.........510.........520.........530.........540.........550.........560.......
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Query Sequence  ADKLEQEKVATLEDATEMVNLYRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPEA
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Template Sequence  DADIEKLPYITFPEGSEEHTYLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLE. . . . . . . . . . . . . . . . . . . . . . .
Template Known Secondary structure  TT






TTSTTS
SS


SS

S





GGGGS.......................
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   430.........440.........450.........460.........470.........480......
 
   568.570.........580.........590.........600........ .610.........620.........630.........640....
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Query Sequence  VEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEG. . . IPVRLSGEDSGRGTFFHRHAVIHNQSNGSTYTPLQH
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Template Sequence  . . . . . . . . . . . . . . . . . . EQSKEISTTIAFVRALNVMLKNKSIKDRLVPIIADEARTF. . . . . . . . . . . GMEGLFRQIGI
Template Known Secondary structure  ..................

SS
B
TT
TTTTTTSS
TGGG...........T

B
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   487..490.........500.........510.........520...... ...530.......
 
   645....650.........660.........670... ......680.........690.........700.........710........
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Query Sequence  IHNGQGAFRVWDSVLSEEAVLAFEYGYAT. . . . . . AEPRTLTIWEAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLL
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Template Sequence  YSPEDEKGQILQEGINELGAGCSWLAAATSYSTNNLPMIPFY. . . IYYSMFGFQR. . . IGDLCWAAGDQQ. . ARGFLIGG
Template Known Secondary structure  B


BTT

B

S
TTS


...GGGS...TT..


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   538.540.........550.........560.........570......... 580......... 590.........600. ........
 
   719720 .........730.........740.........750.........760....... ..770.........780.........790..
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Query Sequence  PH. . . GYEGQGPEHSSARLERYLQLCAEQNMQVCVPSTPAQVYHMLRRQALR. . . GMRRPLVVMSPKSLLRHPLAVSSLE
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Template Sequence  TSGRTTLNGEGLQH. ED. GHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEKQENVYYYITTLNENYHMPAMPE.
Template Known Secondary structure  S
STTTSTTT
TTT.

.S
TTSTT


SSTT





S

B





T.
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   627..630.........640 .. .......650.........660.........670.........680.........690.........700...
 
   793......800.........810.........820.........830... ... ...840.........850.........
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Query Sequence  ELANGTFLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRR. KNN. QHDVAIVRIEQLYPFPHKAMQEV
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Template Sequence  GAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVREAAEILAKDYGVGSDVYSVTSFTELARDGQDCE
Template Known Secondary structure  T
T

SS
GGG
S
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   704.....710.........720.........730.........740.........750.........760.........770..
 
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No model constructed - rank, confidence too low




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Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
 
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