Secondary structure and disorder prediction

		1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	K	I	R	S	L	S	R	F	V	L	A	S	T	M	F	A	S	F	T	A	S	A	V	P	G	L	W	Q	Q	G	Y	G	Q	G	N	A	E	Y	S	V	T	D	A	S	G	K	M	F	T	I	N	C	T	G	N	P	D	Q	N
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?									?	?	?	?	?			?		?			?																														?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		G	I	Y	Q	H	S	V	F	L	I	L	A	G	D	K	T	V	S	S	H	D	D	S	T	G	I	T	V	V	M	D	H	K	Q	Y	A	I	P	S	T	L	G	W	R	N	G	D	N	A	W	F	S	F	I	M	D	I	R	K	A
Secondary structure
SS confidence
Disorder															?					?	?		?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.
Sequence		R	Q	F	D	V	Y	V	N	D	Q	K	V	G	S	F	N	P	D	V	R	N	A	Q	K	V	L	P	T	L	A	D	C	T	N	D
Secondary structure
SS confidence
Disorder													?	?	?	?	?	?	?	?	?	?									?	?	?	?	?	?
Disorder confidence

Confidence Key
High(9)											Low (0)

?	Disordered
	Alpha helix
	Beta strand

Domain analysis

Hover over an aligned region to see model and summary info

Please note, only up to the top 20 hits are modelled to reduce computer load

Rank Aligned region

PDB 1q56 chain A Region: 33 - 147 Aligned: 112 Modelled: 115 Confidence: 46.5% Identity: 11% Fold: Concanavalin A-like lectins/glucanases Superfamily: Concanavalin A-like lectins/glucanases Family: Laminin G-like module Phyre2 PDB 1wmx chain A Region: 30 - 134 Aligned: 94 Modelled: 105 Confidence: 40.4% Identity: 20% Fold: Galactose-binding domain-like Superfamily: Galactose-binding domain-like Family: Family 30 carbohydrate binding module, CBM30 (PKD repeat) Phyre2 PDB 3pve chain A Region: 33 - 148 Aligned: 112 Modelled: 116 Confidence: 27.6% Identity: 12% PDB header:transcription Chain: A: PDB Molecule:agrin, agrn protein; PDBTitle: crystal structure of the g2 domain of agrin from mus musculus Phyre2 PDB 1uzv chain A Region: 122 - 136 Aligned: 15 Modelled: 15 Confidence: 27.0% Identity: 27% Fold: Calcium-mediated lectin Superfamily: Calcium-mediated lectin Family: Calcium-mediated lectin Phyre2 PDB 2kfw chain A Region: 59 - 139 Aligned: 70 Modelled: 81 Confidence: 25.8% Identity: 13% PDB header:isomerase Chain: A: PDB Molecule:fkbp-type peptidyl-prolyl cis-trans isomerase PDBTitle: solution structure of full-length slyd from e.coli Phyre2 PDB 1pz7 chain A Region: 33 - 141 Aligned: 108 Modelled: 109 Confidence: 22.9% Identity: 10% Fold: Concanavalin A-like lectins/glucanases Superfamily: Concanavalin A-like lectins/glucanases Family: Laminin G-like module Phyre2 PDB 2chh chain A domain 1 Region: 122 - 136 Aligned: 15 Modelled: 15 Confidence: 22.0% Identity: 20% Fold: Calcium-mediated lectin Superfamily: Calcium-mediated lectin Family: Calcium-mediated lectin Phyre2 PDB 1ylx chain A domain 1 Region: 44 - 94 Aligned: 48 Modelled: 51 Confidence: 20.0% Identity: 21% Fold: N domain of copper amine oxidase-like Superfamily: GK1464-like Family: GK1464-like Phyre2 PDB 2boi chain A Region: 122 - 143 Aligned: 20 Modelled: 22 Confidence: 17.9% Identity: 10% PDB header:lectin Chain: A: PDB Molecule:cv-iil lectin; PDBTitle: 1.1a structure of chromobacterium violaceum lectin cv2l in2 complex with alpha-methyl-fucoside Phyre2 PDB 1wmx chain B Region: 34 - 134 Aligned: 87 Modelled: 92 Confidence: 16.6% Identity: 23% Fold: Galactose-binding domain-like Superfamily: Galactose-binding domain-like Family: Family 30 carbohydrate binding module, CBM30 (PKD repeat) Phyre2 PDB 2d3n chain A domain 1 Region: 35 - 99 Aligned: 62 Modelled: 65 Confidence: 16.4% Identity: 16% Fold: Glycosyl hydrolase domain Superfamily: Glycosyl hydrolase domain Family: alpha-Amylases, C-terminal beta-sheet domain Phyre2 PDB 2r0f chain A Region: 119 - 145 Aligned: 27 Modelled: 27 Confidence: 16.0% Identity: 11% PDB header:sugar binding protein Chain: A: PDB Molecule:cgl3 lectin; PDBTitle: ligand free structure of fungal lectin cgl3 Phyre2 PDB 2bos chain A Region: 75 - 96 Aligned: 21 Modelled: 22 Confidence: 15.7% Identity: 24% Fold: OB-fold Superfamily: Bacterial enterotoxins Family: Bacterial AB5 toxins, B-subunits Phyre2 PDB 2khg chain A Region: 109 - 120 Aligned: 12 Modelled: 12 Confidence: 15.1% Identity: 42% PDB header:antimicrobial protein Chain: A: PDB Molecule:plnj; PDBTitle: plantaricin j in tfe Phyre2 PDB 2khf chain A Region: 109 - 120 Aligned: 12 Modelled: 12 Confidence: 14.9% Identity: 42% PDB header:antimicrobial protein Chain: A: PDB Molecule:plnj; PDBTitle: plantaricin j in dpc-micelles Phyre2 PDB 1w9i chain A Region: 50 - 102 Aligned: 53 Modelled: 53 Confidence: 14.0% Identity: 15% PDB header:myosin Chain: A: PDB Molecule:myosin ii heavy chain; PDBTitle: myosin ii dictyostelium discoideum motor domain s456y bound2 with mgadp-befx Phyre2 PDB 2eg5 chain C Region: 91 - 136 Aligned: 46 Modelled: 46 Confidence: 13.7% Identity: 20% PDB header:transferase Chain: C: PDB Molecule:xanthosine methyltransferase; PDBTitle: the structure of xanthosine methyltransferase Phyre2 PDB 1qu0 chain A Region: 33 - 138 Aligned: 103 Modelled: 106 Confidence: 13.5% Identity: 9% PDB header:metal binding protein Chain: A: PDB Molecule:laminin alpha2 chain; PDBTitle: crystal structure of the fifth laminin g-like module of the2 mouse laminin alpha2 chain Phyre2 PDB 1qu0 chain D Region: 33 - 138 Aligned: 103 Modelled: 106 Confidence: 13.5% Identity: 9% PDB header:metal binding protein Chain: D: PDB Molecule:laminin alpha2 chain; PDBTitle: crystal structure of the fifth laminin g-like module of the2 mouse laminin alpha2 chain Phyre2 PDB 1ud2 chain A domain 1 Region: 35 - 99 Aligned: 62 Modelled: 65 Confidence: 13.4% Identity: 11% Fold: Glycosyl hydrolase domain Superfamily: Glycosyl hydrolase domain Family: alpha-Amylases, C-terminal beta-sheet domain Phyre2 1 d1q56a_ 2 d1wmxa_ 3 c3pveA_ 4 d1uzva_ 5 c2kfwA_ 6 d1pz7a_ 7 d2chha1 8 d1ylxa1 9 c2boiA_ 10 d1wmxb_ 11 d2d3na1 12 c2r0fA_ 13 d2bosa_ 14 c2khgA_ 15 c2khfA_ 16 c1w9iA_ 17 c2eg5C_ 18 c1qu0A_ 19 c1qu0D_ 20 d1ud2a1 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62

Detailed template information

# Template Alignment Coverage 3D Model Confidence % i.d. Template Information

1 d1q56a_ 46.5 11 Fold:Concanavalin A-like lectins/glucanases Superfamily:Concanavalin A-like lectins/glucanases Family:Laminin G-like module 2 d1wmxa_ 40.4 20 Fold:Galactose-binding domain-like Superfamily:Galactose-binding domain-like Family:Family 30 carbohydrate binding module, CBM30 (PKD repeat) 3 c3pveA_ 27.6 12 PDB header:transcription Chain: A: PDB Molecule:agrin, agrn protein; PDBTitle: crystal structure of the g2 domain of agrin from mus musculus 4 d1uzva_ 27.0 27 Fold:Calcium-mediated lectin Superfamily:Calcium-mediated lectin Family:Calcium-mediated lectin 5 c2kfwA_ 25.8 13 PDB header:isomerase Chain: A: PDB Molecule:fkbp-type peptidyl-prolyl cis-trans isomerase PDBTitle: solution structure of full-length slyd from e.coli 6 d1pz7a_ 22.9 10 Fold:Concanavalin A-like lectins/glucanases Superfamily:Concanavalin A-like lectins/glucanases Family:Laminin G-like module 7 d2chha1 22.0 20 Fold:Calcium-mediated lectin Superfamily:Calcium-mediated lectin Family:Calcium-mediated lectin 8 d1ylxa1 20.0 21 Fold:N domain of copper amine oxidase-like Superfamily:GK1464-like Family:GK1464-like 9 c2boiA_ 17.9 10 PDB header:lectin Chain: A: PDB Molecule:cv-iil lectin; PDBTitle: 1.1a structure of chromobacterium violaceum lectin cv2l in2 complex with alpha-methyl-fucoside 10 d1wmxb_ 16.6 23 Fold:Galactose-binding domain-like Superfamily:Galactose-binding domain-like Family:Family 30 carbohydrate binding module, CBM30 (PKD repeat) 11 d2d3na1 16.4 16 Fold:Glycosyl hydrolase domain Superfamily:Glycosyl hydrolase domain Family:alpha-Amylases, C-terminal beta-sheet domain 12 c2r0fA_ 16.0 11 PDB header:sugar binding protein Chain: A: PDB Molecule:cgl3 lectin; PDBTitle: ligand free structure of fungal lectin cgl3 13 d2bosa_ 15.7 24 Fold:OB-fold Superfamily:Bacterial enterotoxins Family:Bacterial AB5 toxins, B-subunits 14 c2khgA_ 15.1 42 PDB header:antimicrobial protein Chain: A: PDB Molecule:plnj; PDBTitle: plantaricin j in tfe 15 c2khfA_ 14.9 42 PDB header:antimicrobial protein Chain: A: PDB Molecule:plnj; PDBTitle: plantaricin j in dpc-micelles 16 c1w9iA_ 14.0 15 PDB header:myosin Chain: A: PDB Molecule:myosin ii heavy chain; PDBTitle: myosin ii dictyostelium discoideum motor domain s456y bound2 with mgadp-befx 17 c2eg5C_ 13.7 20 PDB header:transferase Chain: C: PDB Molecule:xanthosine methyltransferase; PDBTitle: the structure of xanthosine methyltransferase 18 c1qu0A_ 13.5 9 PDB header:metal binding protein Chain: A: PDB Molecule:laminin alpha2 chain; PDBTitle: crystal structure of the fifth laminin g-like module of the2 mouse laminin alpha2 chain 19 c1qu0D_ 13.5 9 PDB header:metal binding protein Chain: D: PDB Molecule:laminin alpha2 chain; PDBTitle: crystal structure of the fifth laminin g-like module of the2 mouse laminin alpha2 chain 20 d1ud2a1 13.4 11 Fold:Glycosyl hydrolase domain Superfamily:Glycosyl hydrolase domain Family:alpha-Amylases, C-terminal beta-sheet domain 21 d1ulea_ not modelled 13.4 15 Fold:Concanavalin A-like lectins/glucanases Superfamily:Concanavalin A-like lectins/glucanases Family:Galectin (animal S-lectin) 22 d2anua1 not modelled 13.3 16 Fold:7-stranded beta/alpha barrel Superfamily:PHP domain-like Family:PHP domain 23 c2anuA_ not modelled 13.3 16 PDB header:metal binding protein Chain: A: PDB Molecule:hypothetical protein tm0559; PDBTitle: crystal structure of predicted metal-dependent phosphoesterase (php2 family) (tm0559) from thermotoga maritima at 2.40 a resolution 24 d2aw2a1 not modelled 13.3 14 Fold:Immunoglobulin-like beta-sandwich Superfamily:Immunoglobulin Family:V set domains (antibody variable domain-like) 25 d1hvxa1 not modelled 12.9 22 Fold:Glycosyl hydrolase domain Superfamily:Glycosyl hydrolase domain Family:alpha-Amylases, C-terminal beta-sheet domain 26 c2wjsA_ not modelled 12.8 13 PDB header:cell adhesion Chain: A: PDB Molecule:laminin subunit alpha-2; PDBTitle: crystal structure of the lg1-3 region of the laminin alpha22 chain 27 c2fvnA_ not modelled 12.8 28 PDB header:cell adhesion Chain: A: PDB Molecule:protein afad; PDBTitle: the fibrillar tip complex of the afa/dr adhesins from2 pathogen e. coli displays synergistic binding to 5 1 and v3 3 integrins 28 c3hqxA_ not modelled 12.6 12 PDB header:structural genomics, unknown function Chain: A: PDB Molecule:upf0345 protein aciad0356; PDBTitle: crystal structure of protein of unknown function (duf1255,pf06865)2 from acinetobacter sp. adp1 29 d1fyxa_ not modelled 12.6 25 Fold:Flavodoxin-like Superfamily:Toll/Interleukin receptor TIR domain Family:Toll/Interleukin receptor TIR domain 30 c3eo6B_ not modelled 12.5 14 PDB header:structural genomics, unknown function Chain: B: PDB Molecule:protein of unknown function (duf1255); PDBTitle: crystal structure of protein of unknown function (duf1255)2 (afe_2634) from acidithiobacillus ferrooxidans ncib8455 at3 0.97 a resolution 31 c2zw7A_ not modelled 12.5 25 PDB header:transferase Chain: A: PDB Molecule:bleomycin acetyltransferase; PDBTitle: crystal structure of bleomycin n-acetyltransferase complexed2 with bleomycin a2 and coenzyme a 32 d2gjpa1 not modelled 12.3 16 Fold:Glycosyl hydrolase domain Superfamily:Glycosyl hydrolase domain Family:alpha-Amylases, C-terminal beta-sheet domain 33 d1r4pb_ not modelled 11.7 33 Fold:OB-fold Superfamily:Bacterial enterotoxins Family:Bacterial AB5 toxins, B-subunits 34 d1c4qa_ not modelled 11.7 33 Fold:OB-fold Superfamily:Bacterial enterotoxins Family:Bacterial AB5 toxins, B-subunits 35 d2nn8a1 not modelled 11.7 26 Fold:Concanavalin A-like lectins/glucanases Superfamily:Concanavalin A-like lectins/glucanases Family:Galectin (animal S-lectin) 36 d1dyka2 not modelled 11.5 10 Fold:Concanavalin A-like lectins/glucanases Superfamily:Concanavalin A-like lectins/glucanases Family:Laminin G-like module 37 c3nv4A_ not modelled 11.3 20 PDB header:sugar binding protein Chain: A: PDB Molecule:galectin 9 short isoform variant; PDBTitle: crystal structure of human galectin-9 c-terminal crd in complex with2 sialyllactose 38 c3efyB_ not modelled 10.4 12 PDB header:cell cycle Chain: B: PDB Molecule:cif (cell cycle inhibiting factor); PDBTitle: structure of the cyclomodulin cif from pathogenic2 escherichia coli 39 d2axwa1 not modelled 9.4 19 Fold:Common fold of diphtheria toxin/transcription factors/cytochrome f Superfamily:Bacterial adhesins Family:Pilus subunits 40 c3eitB_ not modelled 9.3 12 PDB header:unknown function Chain: B: PDB Molecule:putative atp/gtp binding protein; PDBTitle: the 2.6 angstrom crystal structure of chbp, the cif homologue from2 burkholderia pseudomallei 41 d1fyva_ not modelled 8.9 25 Fold:Flavodoxin-like Superfamily:Toll/Interleukin receptor TIR domain Family:Toll/Interleukin receptor TIR domain 42 d2oyza1 not modelled 8.9 25 Fold:Double-stranded beta-helix Superfamily:RmlC-like cupins Family:VPA0057-like 43 c3b5iB_ not modelled 8.8 22 PDB header:transferase Chain: B: PDB Molecule:s-adenosyl-l-methionine:salicylic acid carboxyl PDBTitle: crystal structure of indole-3-acetic acid methyltransferase 44 c2eg9B_ not modelled 8.7 13 PDB header:hydrolase Chain: B: PDB Molecule:adp-ribosyl cyclase 1; PDBTitle: crystal structure of the truncated extracellular domain of2 mouse cd38 45 d1wdja_ not modelled 8.7 15 Fold:Restriction endonuclease-like Superfamily:Restriction endonuclease-like Family:Hypothetical protein TT1808 (TTHA1514) 46 d1e43a1 not modelled 8.0 11 Fold:Glycosyl hydrolase domain Superfamily:Glycosyl hydrolase domain Family:alpha-Amylases, C-terminal beta-sheet domain 47 c1qu0C_ not modelled 7.6 9 PDB header:metal binding protein Chain: C: PDB Molecule:laminin alpha2 chain; PDBTitle: crystal structure of the fifth laminin g-like module of the2 mouse laminin alpha2 chain 48 d2ftxb1 not modelled 7.4 13 Fold:Kinetochore globular domain-like Superfamily:Kinetochore globular domain Family:Spc24-like 49 c3b9cB_ not modelled 7.4 16 PDB header:unknown function Chain: B: PDB Molecule:hspc159; PDBTitle: crystal structure of human grp crd 50 c2fv4B_ not modelled 7.3 13 PDB header:structural protein, protein binding Chain: B: PDB Molecule:hypothetical 24.6 kda protein in ilv2-ade17 PDBTitle: nmr solution structure of the yeast kinetochore spc24/spc252 globular domain 51 d2gala_ not modelled 6.5 25 Fold:Concanavalin A-like lectins/glucanases Superfamily:Concanavalin A-like lectins/glucanases Family:Galectin (animal S-lectin) 52 c2yroA_ not modelled 6.5 20 PDB header:sugar binding protein Chain: A: PDB Molecule:galectin-8; PDBTitle: solution structure of the c-terminal gal-bind lectin2 protein from human galectin-8 53 d2uz9a1 not modelled 6.4 36 Fold:Composite domain of metallo-dependent hydrolases Superfamily:Composite domain of metallo-dependent hydrolases Family:SAH/MTA deaminase-like 54 c3sh5A_ not modelled 6.1 11 PDB header:metal binding protein Chain: A: PDB Molecule:lg3 peptide; PDBTitle: calcium-bound laminin g like domain 3 from human perlecan 55 c2c5dA_ not modelled 6.1 9 PDB header:signaling protein/receptor Chain: A: PDB Molecule:growth-arrest-specific protein 6 precursor; PDBTitle: structure of a minimal gas6-axl complex 56 d1wdjb_ not modelled 6.0 16 Fold:Restriction endonuclease-like Superfamily:Restriction endonuclease-like Family:Hypothetical protein TT1808 (TTHA1514) 57 d2zgwa2 not modelled 5.9 36 Fold:Class II aaRS and biotin synthetases Superfamily:Class II aaRS and biotin synthetases Family:Biotin holoenzyme synthetase 58 c2eayB_ not modelled 5.9 45 PDB header:ligase Chain: B: PDB Molecule:biotin [acetyl-coa-carboxylase] ligase; PDBTitle: crystal structure of biotin protein ligase from aquifex2 aeolicus 59 c3dtdI_ not modelled 5.8 13 PDB header:structural genomics, unknown function Chain: I: PDB Molecule:invasion-associated protein b; PDBTitle: crystal structure of invasion associated protein b from bartonella2 henselae 60 c2xr4A_ not modelled 5.8 30 PDB header:sugar binding protein Chain: A: PDB Molecule:lectin; PDBTitle: c-terminal domain of bc2l-c lectin from burkholderia cenocepacia 61 d2h3ka1 not modelled 5.6 26 Fold:Immunoglobulin-like beta-sandwich Superfamily:NEAT domain-like Family:NEAT domain 62 d1xaua_ not modelled 5.5 15 Fold:Immunoglobulin-like beta-sandwich Superfamily:Immunoglobulin Family:I set domains

Binding site prediction

Due to computational demand, binding site predictions are not run for batch jobs

If you want to predict binding sites, please manually submit your model of choice to 3DLigandSite

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Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
If you use the binding site predictions from 3DLigandSite, please also cite:
3DLigandSite: predicting ligand-binding sites using similar structures.
Wass MN, Kelley LA and Sternberg MJ Nucleic Acids Research 38, W469-73 (2010) [PubMed]
© Structural Bioinformatics Group Imperial College London Lawrence Kelley, Benjamin Jefferys Disclaimer Terms and Conditions	Component software Template detection: HHpred 1.51 Secondary structure prediction: Psi-pred 2.5 Disorder prediction: Disopred 2.4 Transmembrane prediction: Memsat_SVM Multi-template modelling and ab initio: Poing 1.0