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Job DescriptionP0AFG8
Confidence100.00%DateThu Jan 5 11:26:11 GMT 2012
Rank1Aligned Residues801
% Identity100%Templatec2g28A_
PDB info PDB header:oxidoreductaseChain: A: PDB Molecule:pyruvate dehydrogenase e1 component; PDBTitle: e. coli pyruvate dehydrogenase h407a variant2 phosphonolactylthiamin diphosphate complex
Resolution1.85 Å

  Insertion relative to template
  Deletion relative to template
  Catalytic residue from the CSA
 
Detailed help on interpreting your alignment


   57..60.........70.........80.........90.........100.........110.........120.........130......
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Query Sequence  ISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDLELGGHMASFQSSATIYDVCFNHFFRARNEQDGGD
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Template Conservation                                
      
          


 
  
    
   
             
 
Template Sequence  ISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDLELGGHMASFQSSATIYDVCFNHFFRARNEQDGGD
Template Known Secondary structure 


SS
SS
GGG




S
TT
S




ST




SSS


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   56...60.........70.........80.........90.........100.........110.........120.........130.....
 
   137..140.........150.........160.........170.........180.........190.........200.........210......
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Query Sequence  LVYFQGHISPGVYARAFLEGRLTQEQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRG
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Template Conservation   
   

 
  


 
 
 
      
   

     
  


        
   

 

 


 
 
 


        
Template Sequence  LVYFQGHISPGVYARAFLEGRLTQEQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRG
Template Known Secondary structure 

GGGTTSS
TTBT

TTSS
B
SS

TTTSTTT





STTTT
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   136...140.........150.........160.........170.........180.........190.........200.........210.....
 
   217..220.........230.........240.........250.........260.........270.........280.........290......
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Query Sequence  LKDTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKIINELEGIFEGAGWNVIKVMWG
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Template Conservation       
  
  






 

   

   

   
 


 
 
 
  


  
         
   
 
 

 
  
  
Template Sequence  LKDTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKIINELEGIFEGAGWNVIKVMWG
Template Known Secondary structure  S


TT

TGGGGSTTTT
TT
SB
SSSBSSTTS
TT

B
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   216...220.........230.........240.........250.........260.........270.........280.........290.....
 
   297..300.........310.........320.........330.........340.........350.........360.........370......
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Query Sequence  SRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYVREHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKK
Query Conservation     
 
       

        
   
     
    
       
    

    
  
     



   
  
   
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Template Conservation               

                                                   



   
  
   
Template Sequence  SRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYVREHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKK
Template Known Secondary structure  GGG

S
S
TTS
TGGGSTTTS
T

BGGG
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   296...300.........310.........320.........330.........340.........350.........360.........370.....
 
   377..380.........390.........400.........410.........420.........430.........440.........450......
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Query Sequence  AQETKGKATVILAHTIKGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHTYLHAQR
Query Conservation 
      

 
 
 
 

 
 
   
      

 

  


   
  

 
   
 
             
        
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Template Conservation 
      



   
 

 
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Template Sequence  AQETKGKATVILAHTIKGYGMG. . . . . . . . . . . . . DAAMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHTYLHAQR
Template Known Secondary structure 

SS


TTTT
T.............T


TT
S

TT






TTS
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   376...380.........390....... ..400.........410.........420.........430.........440..
 
   457..460.........470.........480.........490.........500.........510.........520.........530......
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Query Sequence  QKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLKNKSIKDRLVPIIADEARTFGMEGLFRQI
Query Conservation    
    
            
                


 
 
  
  
 
  
    

  




 

 
       
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Template Conservation         
                             


 
 
  
  
         

  



  

 
       
Template Sequence  QKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLKNKSIKDRLVPIIADEARTFGMEGLFRQI
Template Known Secondary structure  TTS
SS


SS

S





GGGGS

SS
B
TT
TTTTTTSS
TGGGT
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   456...460.........470.........480.........490.........500.........510.........520.........530.....
 
   537..540.........550.........560.........570.........580.........590.........600.........610......
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Query Sequence  GIYSPNGQQYTPQDREQVAYYKEDEKGQILQEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAG
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Template Conservation      .................     

 
  

 
      
 
 
      
 

 
    
  
             
Template Sequence  GIYS. . . . . . . . . . . . . . . . . PEDEKGQILQEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAG
Template Known Secondary structure 
BB
.................

BTT

B

S
TTS


GGGS
Template Predicted Secondary structure 



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   536... 540.........550.........560.........570.........580.........590........
 
   617..620.........630.........640.........650.........660.........670.........680.........690......
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Query Sequence  DQQARGFLIGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEKQENVYYYITTLNEN
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Template Conservation        
  
 
  

  
 

 


 

 

 

 



 
  



 
       

            
        
Template Sequence  DQQARGFLIGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEKQENVYYYITTLNEN
Template Known Secondary structure  TT


S
STTTSTTT
TTT

S
TTSTT


SSTT





S

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   616...620.........630.........640.........650.........660.........670.........680.........690.....
 
   697..700.........710.........720.........730.........740.........750.........760.........770......
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Query Sequence  YHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVREAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWN
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Template Conservation                  
 
          





 

 
  

 

  
  
 

   

   
   

          
Template Sequence  YHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVREAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWN
Template Known Secondary structure  B





TT
T

SS
GGG
S
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   696...700.........710.........720.........730.........740.........750.........760.........770.....
 
   777..780.........790.........800.........810.........820.........830.........840.........850......
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Query Sequence  MLHPLETPRVPYIAQVMNDAPAVASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
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Template Conservation                  

          
  
 
 
           

 
 

 

    
    

    

  

  
Template Sequence  MLHPLETPRVPYIAQVMNDAPAVASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
Template Known Secondary structure 
TTS






SS

SS
TTTGGG

SS



S



S
TT
S
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   776...780.........790.........800.........810.........820.........830.........840.........850.....
 
   857..860.........870.........880.......
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Query Sequence  LAKRGEIDKKVVADAIAKFNIDADKVNPRLA
Query Conservation 

  
      
  
   
            
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Template Conservation 

                             
Template Sequence  LAKRGEIDKKVVADAIAKFNIDADKVNPRLA
Template Known Secondary structure  TSS
TT

TTS

TTT
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   856...860.........870.........880......
 
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Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
 
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Lawrence Kelley, Benjamin Jefferys 
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