Return to main results Retrieve Phyre Job Id

Job DescriptionP27302
Confidence100.00%DateThu Jan 5 11:43:48 GMT 2012
Rank11Aligned Residues629
% Identity24%Templatec2g28A_
PDB info PDB header:oxidoreductaseChain: A: PDB Molecule:pyruvate dehydrogenase e1 component; PDBTitle: e. coli pyruvate dehydrogenase h407a variant2 phosphonolactylthiamin diphosphate complex
Resolution1.85 Å

  Insertion relative to template
  Deletion relative to template
  Catalytic residue from the CSA
 
Detailed help on interpreting your alignment


   1........10.........20... ......30.........40.........50.........60.........70....
Predicted Secondary structure 


......

























Query SS confidence 






















. . . . . .


















































Query Sequence  MSSRKELANAIRALSMDAVQKAK. . . . . . SGHPGAPMGMADIAEVLWRDFLKHNPQNPSWADRDRFVLSNGHGSMLIYSL
Query Conservation        

  

         
 ...... 

 
  
   

  

           
    


 
   

     

 
Alig confidence 






















......






















....























Template Conservation             
      
          


 
  
    
   
      ....       
  
   

 
  


 
Template Sequence  LELERRIRSAIRWNAIMTVLRASKKDLELGGHMASFQSSATIYDVCFNHFFR. . . . ARNEQDGGDLVYFQGHISPGVYAR
Template Known Secondary structure  TT
S




ST

....


SSS




GGG
Template Predicted Secondary structure 















....












Template SS confidence 















































































   75....80.........90.........100.........110.........120...... ...130.........140.........150
 
   75....80.........90... ......100........ .110.........120.........130.........140.........150.
Predicted Secondary structure 







..














.















Query SS confidence 


















. .














.










































Query Sequence  LHLTGYDLPMEELKNFRQL. . HSKTPGHPEVGYTAG. VETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYA
Query Conservation    
 
       
   
  ..     
 
     
 .    

 

 


 
 
 
 
                   
  
Alig confidence 





.











..














.






























...








Template Conservation 
 
 
 .     
   

     
  


        
   

 

 


 
 
 


          ...   
  
  
Template Sequence  AFLEGR. LTQEQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK. . . DTSKQTVYA
Template Known Secondary structure  TTS.S
TTBT

TTSS
B
SS

TTTSTTT





STTTTS
...

TT

Template Predicted Secondary structure 


.


































...




Template SS confidence 















































































   151..... ...160.........170.........180.........190.........200.........210....... ..220......
 
   152.......160.........170.........180.........190........ .200.........210.....
Predicted Secondary structure 





















..



..............
Query SS confidence 














































. .
















. . . . . . . . . . . . . .
Query Sequence  FMGDGCMMEGISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFT. . DDTAMRFEAYGWHVIRD. . . . . . . . . . . . . .
Query Conservation 




 
 

   



 
    
  

 
 


  


        ..        
 
      ..............
Alig confidence 














































..
















..............
Template Conservation 






 

   

   

   
 


 
 
 
  


  
         
   
 
 

 
  
  
           
Template Sequence  FLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKIINELEGIFEGAGWNVIKVMWGSRWDELLRKDT
Template Known Secondary structure  TGGGGSTTTT
TT
SB
SSSBSSTTS
TT

B
GGG

Template Predicted Secondary structure 




































Template SS confidence 















































































   227..230.........240.........250.........260.........270.........280.........290.........300......
 
   216...220.........230.........240.
Predicted Secondary structure  ......................................................








Query SS confidence  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

























Query Sequence  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . IDGHDAASIKRAVEEARAVTDKPSLL
Query Conservation  ...................................................... 

 
   
  
   
      
  
Alig confidence  ......................................................

























Template Conservation    

                                                   



   
  
   
      



Template Sequence  SGKLIQLMNETVDGDYQTFKSKDGAYVREHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVI
Template Known Secondary structure  S
S
TTS
TGGGSTTTS
T

BGGG


SS
Template Predicted Secondary structure 















































Template SS confidence 















































































   307..310.........320.........330.........340.........350.........360.........370.........380......
 
   242.......250.........260.........270.........280.........290.........300.........310.........320.
Predicted Secondary structure 































Query SS confidence 















































































Query Sequence  MCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQLGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQE
Query Conservation     
 

 
          
             
 
                                         
  
Alig confidence 









...........























































...
Template Conservation     
 

 
 ...........               
                                     
  ...
Template Sequence  LAHTIKGYGM. . . . . . . . . . . GDAAMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHTYLHAQRQKLHGYLPSR. . .
Template Known Secondary structure 

TTTT
...........TT


TT
S

TT






TTSTTS
SS

...
Template Predicted Secondary structure 





...........























...
Template SS confidence 















































































   387..390...... ...400.........410.........420.........430.........440.........450..
 
   322.......330.........340.........350.........360........ .370.........380.........390........
Predicted Secondary structure 


























...

















Query SS confidence 














































. . .





























Query Sequence  AAEFTRRMKGEMPSDFDAKAKEFIAKLQANPAKIASRKASQNAIEAF. . . GPLLPEFLGGSADLAPSNLTLWSGSKAINE
Query Conservation                                    
        
   ...           

   
             
Alig confidence  .......







































...

















.....






Template Conservation  .......                           


 
 
  
  
         

  



  

..... 
     
Template Sequence  . . . . . . . QPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLKNKSIKDRLVPIIADEARTF. . . . . GMEGLFR
Template Known Secondary structure  .......
SS

S





GGGGS

SS
B
TT
TTTTTTSS
TGGG.....T
Template Predicted Secondary structure  .......


































.....
Template SS confidence 















































































   466...470.........480.........490.........500.........510.........520...... ...530...
 
   399 400.........410.........420..... ....430.........440........ .450.........460...
Predicted Secondary structure  ........








.....

..






Query SS confidence 
. . . . . . . .

























. . . . .






















. .














Query Sequence  D. . . . . . . . AAGNYIHYGVREFGMTAIANGISLHG. . . . . GFLPYTSTFLMFVEYARNAVRMA. . ALMKQRQVMVYTHDS
Query Conservation   ........ 
 
    



   

 
 


  
.....
  
       
  
  



  ..      
  
    
Alig confidence 
........

























.....






















..














Template Conservation             

 
  

 
      
 
 
      
 

 
    
  
                   
  
 
  
Template Sequence  QIGIYSPEDEKGQILQEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFLIGGTSGR
Template Known Secondary structure 
BB


BTT

B

S
TTS


GGGSTT


S
ST
Template Predicted Secondary structure 





























Template SS confidence 















































































   534.....540.........550.........560.........570.........580.........590.........600.........610...
 
   464.....470.........480.........490.........500......... 510.........520........ .530.......
Predicted Secondary structure 



















....













..








Query SS confidence 













































. . . .


















. .








Query Sequence  IGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVER. . . . QDGPTALILSRQNLAQQER. . TEEQLANIA
Query Conservation     
 

 


   


 

 

   
  
 
  
    
  
   ....   
  

  
        ..         
Alig confidence 













































....


















..








Template Conservation 
  
 

 


 

 

 

 



 
  



 
       

            
                       
Template Sequence  TTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEKQENVYYYITTLNENYHMPAMPEGAEEGIR
Template Known Secondary structure  TTSTTT
TTT

S
TTSTT


SSTT





S

B





TT
Template Predicted Secondary structure 














































Template SS confidence 















































































   631........640.........650.........660.........670.........680.........690.........700.........710
 
   538.540...... ...550.........560.........570.... .....580.........590.........600. ...
Predicted Secondary structure 


.







.











...........

Query SS confidence 








.



























.


























. . . . . . . . . . .


Query Sequence  RGGYVLKDC. AGQPELIFIATGSEVELAVAAYEKLTAE. GVKARVVSMPSTDAFDKQDAAYRESVL. . . . . . . . . . . PKA
Query Conservation   
       .    

 

  
  
  

 

  
   .

   

      
 
           ...........   
Alig confidence 








.



























.


























...........


Template Conservation   
 
          





 

 
  

 

  
  
 

   

   
   

                         
Template Sequence  KGIYKLETIEGSKGKVQLLGSGSILRHVREAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQ
Template Known Secondary structure  T

SS
GGG
S

TTS




Template Predicted Secondary structure 




























Template SS confidence 















































































   711........720.........730.........740.........750.........760.........770.........780.........790
 
   605....610... ......620........ .630.........640.........650.........660...
Predicted Secondary structure 


..




.....













Query SS confidence 








. .














. . . . .


































Query Sequence  VTARVAVEA. . GIADYWYKYVGLNGA. . . . . IVGMTTFGESAPAELLFEEFGFTVDNVVAKAKELL
Query Conservation 
  
    
..
              .....   
  
   
    
    

    
   
   
Alig confidence 








..














.....


































Template Conservation   

          
  
 
 
           

 
 

 

    
    

    

  

  
Template Sequence  VMNDAPAVASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGEL
Template Known Secondary structure 

SS

SS
TTTGGG

SS



S



S
TT
S
Template Predicted Secondary structure 


























Template SS confidence 

































































   791........800.........810.........820.........830.........840.........850......
 
Download:Text version FASTA pairwise alignment 3D Model in PDB format

View in Jmol

Send structure to FirstGlance for more viewing options


Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
 
© Structural Bioinformatics Group, Imperial College, London
Lawrence Kelley, Benjamin Jefferys 
Disclaimer
Terms and Conditions