Secondary structure and disorder prediction

		1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		I	S	A	L	V	I	C	I	V	V	C	L	S	W	G	V	N	H	Y	R	D	N	A	I	A	Y	K	E	Q	R	D	K	N	A	R	E	L	K	L	A	N	A	A	I	T	E	M	Q	M	R	Q	R	D	V	A	A	L	D	A	K
Secondary structure
SS confidence
Disorder		?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		Y	T	K	E	L	A	D	A	K	A	E	N	D	A	L	R	D	D	V	A	A	G	R	R	R	L	H	I	K	A	V	C	Q	S	V	R	E	A	T	T	A	S	G	V	D	N	A	A	S	P	R	L	A	D	T	A	E	R	D	Y
Secondary structure
SS confidence
Disorder																					?	?		?									?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.
Sequence		F	T	L	R	E	R	L	V	M	M	Q	A	Q	L	E	G	A	Q	Q	Y	I	T	E	Q	C	L	K
Secondary structure
SS confidence
Disorder																										?	?	?
Disorder confidence

Confidence Key
High(9)											Low (0)

?	Disordered
	Alpha helix
	Beta strand

Domain analysis

Hover over an aligned region to see model and summary info

Please note, only up to the top 20 hits are modelled to reduce computer load

Rank Aligned region

PDB 1n73 chain A Region: 111 - 144 Aligned: 34 Modelled: 34 Confidence: 62.8% Identity: 29% PDB header:blood clotting Chain: A: PDB Molecule:fibrin alpha-1 chain; PDBTitle: fibrin d-dimer, lamprey complexed with the peptide ligand: gly-his-2 arg-pro-amide Phyre2 PDB 3ghg chain D Region: 111 - 144 Aligned: 34 Modelled: 34 Confidence: 36.0% Identity: 12% PDB header:blood clotting Chain: D: PDB Molecule:fibrinogen alpha chain; PDBTitle: crystal structure of human fibrinogen Phyre2 PDB 1m1j chain A Region: 111 - 144 Aligned: 34 Modelled: 34 Confidence: 29.9% Identity: 24% PDB header:blood clotting Chain: A: PDB Molecule:fibrinogen alpha subunit; PDBTitle: crystal structure of native chicken fibrinogen with two different2 bound ligands Phyre2 PDB 3m9b chain K Region: 43 - 80 Aligned: 38 Modelled: 38 Confidence: 27.9% Identity: 29% PDB header:chaperone Chain: K: PDB Molecule:proteasome-associated atpase; PDBTitle: crystal structure of the amino terminal coiled coil domain and the2 inter domain of the mycobacterium tuberculosis proteasomal atpase mpa Phyre2 PDB 1w0b chain A Region: 3 - 46 Aligned: 44 Modelled: 44 Confidence: 25.5% Identity: 20% Fold: Spectrin repeat-like Superfamily: Alpha-hemoglobin stabilizing protein AHSP Family: Alpha-hemoglobin stabilizing protein AHSP Phyre2 PDB 2c9l chain Z Region: 60 - 76 Aligned: 17 Modelled: 17 Confidence: 22.4% Identity: 53% PDB header:viral protein Chain: Z: PDB Molecule:bzlf1 trans-activator protein; PDBTitle: structure of the epstein-barr virus zebra protein Phyre2 PDB 1z8u chain A domain 1 Region: 3 - 46 Aligned: 44 Modelled: 44 Confidence: 21.3% Identity: 20% Fold: Spectrin repeat-like Superfamily: Alpha-hemoglobin stabilizing protein AHSP Family: Alpha-hemoglobin stabilizing protein AHSP Phyre2 PDB 2w6b chain A Region: 24 - 67 Aligned: 44 Modelled: 44 Confidence: 19.4% Identity: 23% PDB header:signaling protein Chain: A: PDB Molecule:rho guanine nucleotide exchange factor 7; PDBTitle: crystal structure of the trimeric beta-pix coiled-coil2 domain Phyre2 PDB 1pnb chain A Region: 131 - 144 Aligned: 14 Modelled: 14 Confidence: 17.6% Identity: 36% PDB header:seed storage protein Chain: A: PDB Molecule:napin bnib; PDBTitle: structure of napin bnib, nmr, 10 structures Phyre2 PDB 1hf9 chain B Region: 56 - 81 Aligned: 26 Modelled: 26 Confidence: 14.3% Identity: 19% PDB header:atpase inhibitor Chain: B: PDB Molecule:atpase inhibitor (mitochondrial); PDBTitle: c-terminal coiled-coil domain from bovine if1 Phyre2 PDB 3klt chain B Region: 21 - 81 Aligned: 61 Modelled: 61 Confidence: 13.0% Identity: 18% PDB header:structural protein Chain: B: PDB Molecule:vimentin; PDBTitle: crystal structure of a vimentin fragment Phyre2 PDB 1m45 chain B Region: 132 - 147 Aligned: 16 Modelled: 16 Confidence: 11.4% Identity: 13% PDB header:cell cycle protein Chain: B: PDB Molecule:iq2 motif from myo2p, a class v myosin; PDBTitle: crystal structure of mlc1p bound to iq2 of myo2p, a class v2 myosin Phyre2 PDB 2kl5 chain A Region: 133 - 145 Aligned: 13 Modelled: 13 Confidence: 11.1% Identity: 31% PDB header:structural genomics, unknown function Chain: A: PDB Molecule:uncharacterized protein yutd; PDBTitle: solution nmr structure of protein yutd from b.subtilis, northeast2 structural genomics consortium target sr232 Phyre2 PDB 2a93 chain B Region: 61 - 81 Aligned: 21 Modelled: 21 Confidence: 10.5% Identity: 29% PDB header:leucine zippers Chain: B: PDB Molecule:c-myc-max heterodimeric leucine zipper; PDBTitle: nmr solution structure of the c-myc-max heterodimeric2 leucine zipper, 40 structures Phyre2 PDB 1jb0 chain F Region: 1 - 19 Aligned: 19 Modelled: 19 Confidence: 9.7% Identity: 16% Fold: Single transmembrane helix Superfamily: Subunit III of photosystem I reaction centre, PsaF Family: Subunit III of photosystem I reaction centre, PsaF Phyre2 PDB 2o01 chain F Region: 1 - 19 Aligned: 19 Modelled: 19 Confidence: 9.4% Identity: 11% PDB header:photosynthesis Chain: F: PDB Molecule:photosystem i reaction center subunit iii, PDBTitle: the structure of a plant photosystem i supercomplex at 3.42 angstrom resolution Phyre2 PDB 3h32 chain A Region: 123 - 144 Aligned: 22 Modelled: 22 Confidence: 9.0% Identity: 14% PDB header:blood clotting Chain: A: PDB Molecule:fibrinogen alpha chain; PDBTitle: crystal structure of d-dimer from human fibrin complexed with gly-his-2 arg-pro-tyr-amide Phyre2 PDB 3gxv chain D Region: 132 - 146 Aligned: 15 Modelled: 15 Confidence: 8.8% Identity: 40% PDB header:hydrolase/replication Chain: D: PDB Molecule:replicative dna helicase; PDBTitle: three-dimensional structure of n-terminal domain of dnab2 helicase from helicobacter pylori and its interactions with3 primase Phyre2 PDB 1go4 chain F Region: 54 - 81 Aligned: 28 Modelled: 28 Confidence: 7.8% Identity: 18% PDB header:cell cycle Chain: F: PDB Molecule:mad1 (mitotic arrest deficient)-like 1; PDBTitle: crystal structure of mad1-mad2 reveals a conserved mad22 binding motif in mad1 and cdc20. Phyre2 PDB 1s0p chain A Region: 134 - 143 Aligned: 10 Modelled: 10 Confidence: 7.5% Identity: 30% Fold: N-terminal domain of adenylylcyclase associated protein, CAP Superfamily: N-terminal domain of adenylylcyclase associated protein, CAP Family: N-terminal domain of adenylylcyclase associated protein, CAP Phyre2 1 c1n73A_ 2 c3ghgD_ 3 c1m1jA_ 4 c3m9bK_ 5 d1w0ba_ 6 c2c9lZ_ 7 d1z8ua1 8 c2w6bA_ 9 c1pnbA_ 10 c1hf9B_ 11 c3kltB_ 12 c1m45B_ 13 c2kl5A_ 14 c2a93B_ 15 d1jb0f_ 16 c2o01F_ 17 c3h32A_ 18 c3gxvD_ 19 c1go4F_ 20 d1s0pa_ 21 22 23 24 25

Detailed template information

# Template Alignment Coverage 3D Model Confidence % i.d. Template Information

1 c1n73A_ 62.8 29 PDB header:blood clotting Chain: A: PDB Molecule:fibrin alpha-1 chain; PDBTitle: fibrin d-dimer, lamprey complexed with the peptide ligand: gly-his-2 arg-pro-amide 2 c3ghgD_ 36.0 12 PDB header:blood clotting Chain: D: PDB Molecule:fibrinogen alpha chain; PDBTitle: crystal structure of human fibrinogen 3 c1m1jA_ 29.9 24 PDB header:blood clotting Chain: A: PDB Molecule:fibrinogen alpha subunit; PDBTitle: crystal structure of native chicken fibrinogen with two different2 bound ligands 4 c3m9bK_ 27.9 29 PDB header:chaperone Chain: K: PDB Molecule:proteasome-associated atpase; PDBTitle: crystal structure of the amino terminal coiled coil domain and the2 inter domain of the mycobacterium tuberculosis proteasomal atpase mpa 5 d1w0ba_ 25.5 20 Fold:Spectrin repeat-like Superfamily:Alpha-hemoglobin stabilizing protein AHSP Family:Alpha-hemoglobin stabilizing protein AHSP 6 c2c9lZ_ 22.4 53 PDB header:viral protein Chain: Z: PDB Molecule:bzlf1 trans-activator protein; PDBTitle: structure of the epstein-barr virus zebra protein 7 d1z8ua1 21.3 20 Fold:Spectrin repeat-like Superfamily:Alpha-hemoglobin stabilizing protein AHSP Family:Alpha-hemoglobin stabilizing protein AHSP 8 c2w6bA_ 19.4 23 PDB header:signaling protein Chain: A: PDB Molecule:rho guanine nucleotide exchange factor 7; PDBTitle: crystal structure of the trimeric beta-pix coiled-coil2 domain 9 c1pnbA_ 17.6 36 PDB header:seed storage protein Chain: A: PDB Molecule:napin bnib; PDBTitle: structure of napin bnib, nmr, 10 structures 10 c1hf9B_ 14.3 19 PDB header:atpase inhibitor Chain: B: PDB Molecule:atpase inhibitor (mitochondrial); PDBTitle: c-terminal coiled-coil domain from bovine if1 11 c3kltB_ 13.0 18 PDB header:structural protein Chain: B: PDB Molecule:vimentin; PDBTitle: crystal structure of a vimentin fragment 12 c1m45B_ 11.4 13 PDB header:cell cycle protein Chain: B: PDB Molecule:iq2 motif from myo2p, a class v myosin; PDBTitle: crystal structure of mlc1p bound to iq2 of myo2p, a class v2 myosin 13 c2kl5A_ 11.1 31 PDB header:structural genomics, unknown function Chain: A: PDB Molecule:uncharacterized protein yutd; PDBTitle: solution nmr structure of protein yutd from b.subtilis, northeast2 structural genomics consortium target sr232 14 c2a93B_ 10.5 29 PDB header:leucine zippers Chain: B: PDB Molecule:c-myc-max heterodimeric leucine zipper; PDBTitle: nmr solution structure of the c-myc-max heterodimeric2 leucine zipper, 40 structures 15 d1jb0f_ 9.7 16 Fold:Single transmembrane helix Superfamily:Subunit III of photosystem I reaction centre, PsaF Family:Subunit III of photosystem I reaction centre, PsaF 16 c2o01F_ 9.4 11 PDB header:photosynthesis Chain: F: PDB Molecule:photosystem i reaction center subunit iii, PDBTitle: the structure of a plant photosystem i supercomplex at 3.42 angstrom resolution 17 c3h32A_ 9.0 14 PDB header:blood clotting Chain: A: PDB Molecule:fibrinogen alpha chain; PDBTitle: crystal structure of d-dimer from human fibrin complexed with gly-his-2 arg-pro-tyr-amide 18 c3gxvD_ 8.8 40 PDB header:hydrolase/replication Chain: D: PDB Molecule:replicative dna helicase; PDBTitle: three-dimensional structure of n-terminal domain of dnab2 helicase from helicobacter pylori and its interactions with3 primase 19 c1go4F_ 7.8 18 PDB header:cell cycle Chain: F: PDB Molecule:mad1 (mitotic arrest deficient)-like 1; PDBTitle: crystal structure of mad1-mad2 reveals a conserved mad22 binding motif in mad1 and cdc20. 20 d1s0pa_ 7.5 30 Fold:N-terminal domain of adenylylcyclase associated protein, CAP Superfamily:N-terminal domain of adenylylcyclase associated protein, CAP Family:N-terminal domain of adenylylcyclase associated protein, CAP 21 c3n27A_ not modelled 6.9 19 PDB header:viral protein Chain: A: PDB Molecule:fusion glycoprotein f0, linker, fusion glycoprotein f0; PDBTitle: molecular basis of the inhibition of henipa viruses 22 c1f8aB_ not modelled 6.7 12 PDB header:isomerase Chain: B: PDB Molecule:peptidyl-prolyl cis-trans isomerase nima- PDBTitle: structural basis for the phosphoserine-proline recognition2 by group iv ww domains 23 c3gxvC_ not modelled 5.8 32 PDB header:hydrolase/replication Chain: C: PDB Molecule:replicative dna helicase; PDBTitle: three-dimensional structure of n-terminal domain of dnab2 helicase from helicobacter pylori and its interactions with3 primase 24 c1n2dC_ not modelled 5.5 13 PDB header:cell cycle Chain: C: PDB Molecule:iq2 and iq3 motifs from myo2p, a class v myosin; PDBTitle: ternary complex of mlc1p bound to iq2 and iq3 of myo2p, a2 class v myosin 25 c2v4hA_ not modelled 5.5 15 PDB header:transcription Chain: A: PDB Molecule:nf-kappa-b essential modulator; PDBTitle: nemo cc2-lz domain - 1d5 darpin complex

Binding site prediction

Due to computational demand, binding site predictions are not run for batch jobs

If you want to predict binding sites, please manually submit your model of choice to 3DLigandSite

Transmembrane helix prediction

Transmembrane helices have been predicted in your sequence to adopt the topology shown below

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Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
If you use the binding site predictions from 3DLigandSite, please also cite:
3DLigandSite: predicting ligand-binding sites using similar structures.
Wass MN, Kelley LA and Sternberg MJ Nucleic Acids Research 38, W469-73 (2010) [PubMed]
© Structural Bioinformatics Group Imperial College London Lawrence Kelley, Benjamin Jefferys Disclaimer Terms and Conditions	Component software Template detection: HHpred 1.51 Secondary structure prediction: Psi-pred 2.5 Disorder prediction: Disopred 2.4 Transmembrane prediction: Memsat_SVM Multi-template modelling and ab initio: Poing 1.0